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Original Article | Artculo Original
Resumen
pharmacognostical
macromorfologa,
ARTICLE INFO
Received | Recibido: February 2, 2016.
Received in revised form | Recibido en forma corregida: April 7, 2016.
Accepted | Aceptado: April 9, 2016.
Available Online | Publicado en Lnea: April 13, 2016.
Declaration of interests | Declaracin de Intereses: The authors declare no conflict of interest.
Funding | Financiacin: This work was financially supported by the Scientific Research Funds for High Calibre Researchers of Shehezi
University (Project No. RCZX201440) and sponsored by the 12th Five-Year Grand support of Ministry of Science and Technology of the
Peoples Republic of China (2012BAI30B02).
Academic Editor | Editor Acadmico: Gabino Garrido.
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Zhu et al.
INTRODUCTION
Ancient traditional remedies, notably traditional
Chinese medicine and Ayurveda, have been passed
down and refined over their long history of clinical
use (Williamson et al., 2015). The use of herbal medicinal products and supplements, principally for primary health care, has increased tremendously over
the past three decades with not less than 80% of
people worldwide (Ekor, 2014). Viola is the largest genus in the family Violaceae, with 525 - 600 species
in the world, and most species distributed in the
temperate Northern hemisphere (Zhou et al., 2008;
Ning et al., 2012). Many Viola species contain anthocyanins, which have strong antioxidant activities.
Most violas tested and many other plants of the
family Violaceae contain cyclotides, which have a
diverse range of in vitro biological activities, including uterotonic, anti-HIV, antimicrobial, and insecticidal activities (Tang et al., 2010; He et al., 2011; Zhang et al.,
2012). Viola canescens, a species from India, exhibited anti-malarial activity and in vitro activity against
Trypanosoma cruzi (Dua et al., 2011; Verma et al., 2011).
Viola has been evaluated for different clinical indications in human studies. Some clinical trial
showed that the Viola odorata can improve the
cough suppression in children with asthma
(Qasemzadeh et al., 2015), and extract oil showed to be
effective in patients with insomnia (Feyzabadi et al.,
2014).
There are about 120 species of Viola L. genus distributed widely in China, among them Viola tianschanica Maxim. mainly distributed in Xinjiang,
Gansu, Qinghai, Sichuan provinces and Tibet (Chen
et al., 1999; Xinjiangensis, 2011). It was contained in Medicinal Flora of Uygur (Volume I) (Yongmin, 1999).
The whole herb, with taste light bitter and pungent,
cool in nature, is widely used in clearing away heat
and eliminating toxin, reducing fever and inflammation, anti-swelling, moistening lungs to stop a
cough, helping to defecate and so on (Yongmin, 1999).
It was as a substitute of herba Violae (V. yedoensis
Makino) in Xinjiang, mainly in Southern Xinjiang
(Qin et al., 2014).
It was also documented in Chinese Pharmacopoeia Uigur Pharmacopoeia Fascicule, mainly used
in the treatment fever, pyrexia, headache and influenza, acute pleurisy, pneumonia, dry pharynx,
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It smelled slightly aromatic and tasted slightly pungent and bitter (Fig. 2).
Microscopic Identification
Transverse section of V. tianschanica leaves
Through the midrib showed the following tissue
systems:
Epidermal cells, one layer, tightly packed, round
the outside wall thickening. The outside wall of
lower epidermis wave-shaped bending, outside
of which have cuticle thickness.
There were two columns of main veins vascular
bundles, clusters of calcium oxalate were common besides vascular bundles.
Palisade tissue cells were almost broken, without
going through the main vein, individual crystals
were occasionally found in palisade tissue cells
(Fig. 3).
Transverse section of the root
Macroscopical identification
The morphological appearance of herbs was not
complete. It was usually 3 - 7 cm long and focused
between 2 - 4 cm. Taproot was thick. The shape was
conical, and the color was brown to grayish white.
It had irregular longitudinal wrinkles. Its rhizome
was 2 - 4 mm long, and the diameter was 3 mm with
the ring of decorative pattern and petiole residues.
The leaves were shrinking, broken clustered in a
short and thick stems. Complete wet opened leaf
was spoon-shaped or oval. The color was graygreen. Its top was obtusely rounded, and its basic
part was cuneate and decurrent, with a long handle.
The flower was pale blue with five lanceolate sepals
and five obviate symmetrical petals. Capsules were
usually split in a triangle and there were many light
yellow seeds in it. Its quality of material was loose,
and the section was light brown with sparse pores.
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Oval:
Cork layer cells 1-2 layers, rectangle or irregular,
with thickened cell wall. The phelloderm was
composed of 3-4 columns flat parenchyma cells,
tightly packed.
The cortex was very narrow, flat cells, tightly
packed. There were few clusters of calcium oxalate in cells of the cortex. The cracks exist between cortex and phelloderm.
The primary xylem was diarch, centrally located.
Vessels were small and secondary xylem extends
to both sides. Lignified tracheas two layers, in
which there were xylem parenchyma cells. The
diameter of outer layer vessels was big and xylogen is not lignified.
Vascular cambium was not evident. Phloem was
wide, composed of tightly packed cells.
Clusters of calcium oxalate can be found in
phloem and parenchyma cells (Fig. 4).
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Powder microscopy
Yellow-green powder
The anomocytic stoma or anisocytic stoma, subsidiary cells 3 - 4, whose long axis was about 40
m and short axis was about 30 m.
Fibres were long-shuttle shape, mostly broken,
of which completed ones were 400 - 900 m in
length, 65 - 110 m in diameter, 2 - 7 m in
thickness of wall, cavity was not obvious.
Clusters of calcium oxalate were common,
whose edges and corners were broad and obtuse.
Clusters of calcium oxalate, which were sporadic,
have bigger diameters, up to 18 - 60 m. In parenchyma cells, clusters of calcium oxalate were
generally small.
Single cells non-glandular hairs were conical,
200 - 400 m in length, 15 - 20 m in diameter,
with thin walls about 2 - 4 m.
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ing their quality control profile. As per the guidelines of WHO, pharmacognostical standards should
be proposed as a protocol for the diagnosis and authentication of the herbal drugs (Pramanick, 2016).
Moisture content was less than the limited index, explaining dry weather in Xinjiang, it can reach
the standard. Total ash and water soluble ash were
all lower than the standard of Pharmacopoeia of the
People's Republic of China. Acid insoluble ash was
far higher than the standard for the whole herb
medicine in China pharmacopoeia, the possible reason is that herbal medicine is not clean, containing
soil and another inorganic impurity (Table 2).
Physicochemical characteristics
TLC check
Physicochemical studies
Scan of the ultraviolet spectrum
There were three absorption peaks at 204 nm,
266 nm and 351 nm. There may be the presence of
flavonoids in 70% ethanol extracts (Qin et al., 2015)
(Fig. 8).
Phytochemical screening
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Zhu et al.
0.8
0.6
A
B 0.4
S
0.2
0
200
250
300
350
400
Wavelength(nm
12
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Zhu et al.
HCl-Mg reaction
AlCl3 reaction
Microsublimation-vanillic
Libermann-Burchard test
Foam test
Foam 1.5 cm
Tannin
Amino acid
Ninhydrin
Flavanoid
Volatile oil
Alkaloid
Saponin
Total ash
12.0
15.0
3.8
1.5
4.0
8.0
Moisture content
6.5
10.0
Rf 1-Rf 7 = 0.98,
Rf 2 = 0.78,
Rf 3-Rf 8 = 0.74,
Rf 4 = 0.66,
Rf 5 = 0.60,
Rf 6 = 0.42
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CONCLUSIONS
Physicochemical parameters like ash values,
moisture content are all indicators of quality herbal
medicine, which help to determinate the physiological and non-physiological ash, possibility of microbial growth or contamination and presence of impurities respectively. The relative high acid insoluble ash value (3.8%) and a high ratio of water soluble ash content (4.0%) of V. tianschanica Maxim.
indicates that the crude drug contains plenty of
physiological ash and the non-physiological contentit will affect the clinical efficacy of drugs, so it
should pay attention to the control quality of medicinal materials in herbs production.
This research showed that, V. tianschanica Maxim. can be identified by structural features or characteristics of flowers, rhizome and roots, specific
thickening of endothecium cells of clinandrium can
be regarded as distinctive identification character.
In identification of ultraviolet spectrum, there are
three obvious absorption peaks. There are obvious
spots on thin-layer chromatography. All above have
the significance of the identification in pharmacognosy.
Efforts have been made by the authors to bring
out every detail on the macroscopical and microscopical characters of V. tianschanica Maxim. The
study of pharmacognostical features had shown the
standards, which will be useful for the detection of
its identity and authenticity.
It provides reference basis for formulating quality standard of V. tianschanica Maxim., authenticity
of medicinal herbs and resource utilization.
CONFLICT OF INTEREST
The authors declare no conflict of interest.
ACKNOWLEDGEMENT
This work was financially supported by the Scientific Research Funds for High Calibre Researchers of Shehezi University (Project No. RCZX201440). This work was sponsored by the
12th Five-Year Grand support of Ministry of Science and Technology of the Peoples Republic of China (2012BAI30B02).
REFERENCES
Chen J, Wu ZY, Raven PH (1999) Flora of China. Science Press
Beijing. pp. 93-1008
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