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For this research using upstream citarum river for sampling station. Sampling stations were
determined as reference site with condition that have not been disturbed. Conditions surrounding
the sampling station still crowded with riparian vegetation such as spring water and upstream.
Nanti ibu langsung aja sambung ke kalimat berikutnya, tidak perlu pakai kata sambung karena
sudah dimasukkan ke dalamnya.
Untuk gambar agricultural activity dan domestic activity
Next for the deteriorating of river quality because change of land use and pollution still becomes
a problem. Because of that problem the other sampling station determined based on the
utilization of the different areas around the river, such as agricultural activity and domestic
activity.
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One of the biotic components waters often studied the impact of anthropogenic activity in the
river is benthic macroinvertebrates. Aquatic biota is an important component in the biological
assay (bioassessment) in order to evaluate the overall quality of water resources, ecological
functions, as well as the specific influence of anthropogenic activities. The benthic
macroinvertebrates have been found as the most common fauna assemblages for bioassessment.
So this research using.(kata yang ada dalam slide bu).
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Benthic macroinvertebrates community structures and physicochemical parameters were
assessed in seven sampling sites located in Citarum upstream region, West Java, Indonesia.
The study area is on the figure. Reference site (SR) represented as unimpaired or least impaired a
condition as possible, whereas test site (ST) were known to be influenced by varying levels of
anthropogenic stressors.
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There are four (4) reference site on the figure. Spring on the Wayang mountain is one of the
Citarum upstream that flows near protected forest. While spring on Guha mountain, Puncak Cae
mountain, and Halimun mountain locations near shrubs flows directly to the Citarum upstream.
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And then for test site there are three (3) on the figure. Site test known to be influenced by
varying levels. Farm, livestock, and traditional dairy cow are the dominant activity in Cikitu

Village. While plantation and settlements are the dominant activity in Babakan Village and then
rice fields, residential and sand mining are the dominant activity in Wangisagara.
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Major threats on this river are household wastes, agricultural activities, gravel and
sand quarrying, farming and habitat degradation by human activities.

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For sampling design need chemical and physical data collection and benthic macroinvertebrates
sampling.
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For sampling method and analysis using water quality chemical and physical parameter. First
doing sampling and laboratory analysis based on government regulation No. 82 Year 2001 class
II and dominant parameter. And then analysis parameter for subsequent measurement based on
selection.
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And then for benthic macroinvertebrates sampling.(sudah ada dalam slide tulisannya)
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Based on the figure can be seen that the linear regression between pollution index and family
biotic index show the most significant relationship with R = 0,7265.

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