Professional Documents
Culture Documents
Facultad de Medicina
Unidad de Postgrado
Maestra de Fisiologa
MONOGRAFA:
EQUILIBRIO CIDO BASE
Lima
2015
INTRODUCCIN
It is essential for the body to control the concentration of free protons (hydrogen
ions) in the ECF. Although most substances regulated by renal processes exist
at plasma levels in the millimolar range or greater, the normal hydrogen ion
concentration
is a seemingly miniscule 40 nanomolar (1 nanomole is 1 millionth of a
millimole). Even though very small, this level is crucial for body function. Proteins
contain titratable groups that reversibly bind hydrogen ions. As sites on membrane
proteins protonate and deprotonate in response to changes in extracellular pH,
the resulting alteration of local charge density affects the shape, and therefore
the behavior of those proteins. The plasma levels of hydrogen ions are constantly
being altered by a number of processes, including (1) metabolism of ingested food,
(2) secretions of the gastrointestinal (GI) tract, (3) de novo generation of acids and
bases from metabolism of stored fat and glycogen, and (4) changes in the production
of carbon dioxide.
The essence of the physiological response to these changes comes down to
2 processes: (1) matching the excretion of acid-base equivalents to their input, that
is, maintaining balance, and (2) regulating the ratio of weak acids to their conjugate
bases in buffer systems. Buffer systems limit changes in pH to a small range.
The 2 processes of excreting acids and bases, and regulating physiological buffer
concentrations are intimately related, but they are not identical. It is possible to be
in balance even though buffer ratios are inappropriate.
This simple formula provides the basis for measurement of acidity, that
is, the amount of free H+ released from HA at equilibrium in any given setting,
and avoids the confusion that arose with other approaches to describing the
acidbase status of the body fluids such as defining cations as bases and anions
as acids (6,7). The relative ease with which a given molecule in a given milieu
dissociates H+ under equilibrium conditions is a measure of acid strength. In
the mixture of weak acids that comprise biological fluids, the concentration of
H+ ([H+]) is vanishingly small in comparison to HA and A_, and its measurement
requires the use of the pH electrode. The technique for measuring [H+] in
biological solutions, and its nomenclature, is described below.
the range of values of interest is shown in Fig. 1 and Table 1. This conversion
has two main virtues. The first is to make manipulation of the Henderson
Hasselbalch equation (see later) simpler by removing logarithms, and
the second is to call attention to the impact of changes in acidity on mass
balance in any quantitative analysis (1). The two modes of assessing acidity
are used interchangeably in this book.
Hydrogen Ion Activity in Water: Law of Mass Action
In his discussion of the milieu interieur or inner environment of the
body fluids, Claude Bernard (12) stated, Water is the first indispensable
condition of every vital manifestation . . . . The properties of this remarkable
molecule are therefore worthy of consideration. Because of its structure,
water behaves as a dipole with a weak positive charge in the region of
the hydrogen atoms and a weak negative charge in the region of the
oxygen atom. This property of water causes substances whose atoms are
kept together primarily by electrostatic bonds to dissociate into their
component ions when they dissolve in it. In addition, this property of water
causes it to dissociate slightly into H and OH_. Although this dissociation
is minute, it can be measured with the pH electrode. Water dissociation
follows the law of mass action. According to this principle, the cycle
of dissociation and recombination is a continuous and reversible process:
HOH $ H OH_ 5
The H in Eq. (5) actually combines with an undissociated HOH to
produce a hydronium ion, H3O, but by convention the symbol H is used
to designate hydrogen ions in solution (9). At any given moment, a particular
molecule is either dissociated or recombined. The fraction of all such
molecules in one or the other state is stable at equilibrium because the more
molecules dissociate [movement to the right-hand side of Eq. (5)], the stronger
the drive for recombination (movement to the left-hand side), and vice
versa. At equilibrium, conditions are defined as follows:
H_ _ OH__=HOH_ K
0
6
This equation states that at equilibrium the ratio of the concentration
products of the opposing reactions is a predictable value (K0
HOH). K0
HOH is
not a constant, but varies with temperature. Because the high concentration
of undissociated water ([HOH] 55.5 M) varies negligibly in dilute
solutions, the equation simplifies to
H_ _ OH__ K
HOH
10_14 7
The prime symbol indicates that concentrations rather than activities
HOH
Sistema buffer
1HA1_=A1_K
0
2HA2_=A2_K
0
3HA3_=A3_K
0
nHAn_=An_
15
In such a complex solution, the equilibrium value for [H] is determined
by the composite effect of the concentrations of the buffer pairs
and their respective dissociation constants. The beauty of this formulation
is that one can quickly and easily determine the value for [H] in a given
solution from a knowledge of the K0 and concentrations of just one acid
and its conjugate base in the system. Thus, regardless of the complexity of
Describe el pH de los compartimientos corporales y explica los principales sistemas amortiguadores y el equilibrio
cido-bsico