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Mikroskop Elektron
Sutiman B. Sumitro
Guru Besar Biologi Sel
Universitas Brawijaya
Understanding Size
1 metre
Understanding Size
10 centimetres
4/13/2016
Understanding Size
1 centimetre
Understanding Size
100 micrometres
Understanding Size
10 micrometres
4/13/2016
Understanding Size
1 micrometre
Understanding Size
100 nanometres
Understanding Size
10 nanometres
4/13/2016
Understanding Size
1 nanometre
Tahun 2013
4/13/2016
Modern
Physics
Newtonian
Nano Science
Quantum
Physics
Gravitation,
speed
Space and time
Atom and
Subatomic,
particless
Time and
space less
Measurable
dimension,
weigh, and
speed
1 nm
100 nm
4/13/2016
Sample preparation
Tissue Sectioning
4/13/2016
Imaging methods
Contrast formation
Contrast formation in the TEM depends greatly on the mode of operation.
Complex imaging techniques, which utilize the unique ability to change lens
strength or to deactivate a lens, allow for many operating modes
Bright field The most common mode of operation for a TEM is the bright
field imaging mode
Diffraction contrast known as a dark-field image.
Scaning
Transmision
Electon
Microscope
STEM)
TEM can be modified into a
Scanning Transmission
Electron Microscope (STEM)
by the addition of a system
that rasters the beam across
the sample to form the image
4/13/2016
100 nanometres
1 micrometre
10 micrometres
4/13/2016
SEM
NORMAL
OSTEOPOROSIS
25
XRD PATTERN
XRD PATTERN
4/13/2016
200
(nm)
150
100
50
0
HA-Synthetic
Skull Bone
Normal
Cortical Bone
Osteoporosis
28
Crystal Size
Data (15)
D (15)
Crystal size
(nm)
HAp std
1899,15
189,92
S-16
263,31
26,33
S-15
197,43
19,74
S-2
263,85
26,39
S-4
716,83
71,68
M5
790,46
79,05
Bone Skull
88,99
8,90
Normal
Cortical Bone
350,73
35,07
Al
Fe
Ca
Cu
Zn
Mg
30 Ti
10
4/13/2016
Ca
Cu
Zn
Al
Ti
Fe
Mg
31
Osteoporosis
Normal
Periodic
Ca, P
Ca, K, Ti
IV
Cu, Zn
Cu, Zn, Fe
IV
Al, P, Mg, S
III
32
11