Professional Documents
Culture Documents
DOI: 10.1093/jac/dkg293
Advance Access publication 12 June 2003
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5
2003 The British Society for Antimicrobial Chemotherapy
16 200 tons were produced in 20008 of which 70% was used in livestock farming. This is eight times the amount used in human medicine.
Wise estimated total antibiotic market consumption world-wide
to lie between 100 000 and 200 000 tons.9
Unused therapeutic drugs are sometimes disposed of into the
sewage system. If the drugs are not degraded or eliminated during
sewage treatment, in soil or in other environmental compartments,
they will reach surface water and ground water, and, potentially,
drinking water. Unmetabolized antibiotic substances are often
passed into the aquatic environment in wastewater. Antibiotics used
for veterinary purposes or as growth promoters are excreted by the
animals and end up in manure. Manure is used as an agricultural
fertilizer; thus, the antibiotics seep through the soil and enter ground
water (Figure 1). However, very little is known about the occurrence,
fate and risks associated with antibiotics entering the environment
after being used in human and veterinary medicine and as growth
promoters; 95% community use is reported for the UK.4 The figure
for the USA is 75%.9 In Germany, 75% of antimicrobials are used in
the community and 25% in hospitals.
Ciprofloxacin, for example, was found in concentrations of
between 0.7 and 124.5 g/L in hospital effluent.10 Ampicillin was
found in concentrations of between 20 and 80 g/L in the effluent of
a large German hospital.11 Antibiotic concentrations calculated and
measured in hospital effluents are of the same order of magnitude as
the minimum inhibitory concentrations for susceptible pathogenic
bacteria.12 The dilution of hospital effluents by municipal sewage
will lower the concentration of antibiotics only moderately, because
municipal waste water also contains antibiotic substances and disinfectants from households, veterinary sources and to a minor extent
from livestock. Antibiotics have been detected in the g/L range in
municipal sewage, in the effluent of sewage treatment plants (STPs),
in surface water and in ground water.1316 These included quinolones
such as ciprofloxacin, sulphonamides, roxythromycin, dehydrated
erythromycin and others. If antibiotics are used in animal husbandry,
they pass into the soil from manure. Tetracyclines have been detected
in concentrations of up to 0.2 g per kg in soil17 whereas others have
been found in the sediment under fish farms.
Background
Leading article
is used for irrigation, and sewage sludge serves as a fertilizer. This
allows resistant bacteria to enter the food chain directly.
Concentrations below therapeutic levels may play a role in the
selection of resistance and its genetic transfer in certain bacteria.
Exposure of bacteria to sub-therapeutic antimicrobial concentrations
is thought to increase the speed at which resistant strains of bacteria
are selected. Resistance can be transferred to other bacteria living
in other environments such as ground water or drinking water. In
general, knowledge of subinhibitory concentrations and their effects
against environmental bacteria is poor, especially with respect to
resistance. There are a number of recent and older publications about
the mechanisms of very low antibiotic concentrations on the expression of bacterial virulence factors. As for the fate and effects of
antibiotics against bacteria and other organisms in the environment, it
is not clear whether the standardized tests used for risk assessment of
chemicals are appropriate for antibiotics and other pharmaceuticals.
Studies using test systems indicate that various antibiotics remain
active against different groups of bacteria present in waste water.11,12
Effects against algae and daphnids have been reported at surprisingly
low concentrations (5100 g/L).2628
Effects
Antimicrobials may have qualitative and quantitative effects upon
the resident microbial community of sediments, as summarized by
Nygaard et al.22 Resistant bacteria may be selected by antibiotic
substances in hospital effluent, municipal sewage, aeration tanks, the
anaerobic digestion process of STPs or in soil. Furthermore, resistant
bacteria are excreted and discharged into sewage or soil and other
environmental compartments. Resistant and even multi-resistant
pathogenic bacteria have been detected in wastewater and STPs, as
well as in other environmental compartments.2325 Furthermore, in
arid regions, wastewater containing resistant bacteria and antibiotics
Conclusions
Only little is known about the occurrence, fate, effects and risks
associated with the release of antibiotics and other drugs into the
environment. There is still a lack of fundamental data on the occur-
Leading article
References
1. Kmmerer, K. (Ed.). (2001). Pharmaceuticals in the Environment.
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2. The Council of the European Union. (2002). Council Recommendation of 15 November 2001 on the Prudent Use of Antimicrobial Agents
in Human Medicine (Text with EEA relevance). 2002/77/EC. 5 February,
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3. Wise, R., Hart, T., Cars, O. et al. (1998). Antimicrobial resistance
is a major threat to public health. British Medical Journal 317, 60910.
4. House of Lords Select Committee on Science and Technology.
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MA, USA.
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solid-phase extraction and liquid chromatography with fluorescence detection. Analytical Chemistry 73, 36328.