Professional Documents
Culture Documents
2120 A. Introduction
Color in surface and ground waters results primarily from the
presence of natural organic matter, particularly aquatic humic
matter. Humic matter consists of humic and fulvic acids; both
cause a yellow-brown color. Humic acids give a more intense
color, and the presence of iron intensifies the color through the
formation of soluble ferric humates. Suspended particles, especially colloidal-size particles such as clays, algae, iron and
manganese oxides, give waters an appearance of color; they
should be removed before measurement. Industrial wastewaters
can contain lignins, tannins, dyes, and other organic and inorganic chemicals that cause color. Humic materials and the color
caused by these materials are removed from potable water supplies for aesthetic reasons and for health reasons because they
are precursors in the formation of disinfection by-products.
Color also is removed to make water suitable for industrial
applications. Colored industrial wastewaters may require color
removal before discharge into watercourses.
1. Definitions
The term color is used here to mean true color, that is, the
color of water from which turbidity has been removed. Colloidal
and larger suspended particles scatter light interfering with the
determination of true color measurements in Method B and in
the spectrophotometric procedures of Methods C through F. The
term apparent color includes not only color due to substances
3. Bibliography
BLACK, A.P. & R.F. CHRISTMAN. 1963. Characteristics of colored surface
waters. J. Amer. Water Works Assoc. 55:753.
CHRISTMAN, R.F. & M. GHASSEMI. 1966. Chemical nature of organic
color in water. J. Amer. Water Works Assoc. 58:723.
THURMAN, E.M. 1985. Organic Geochemistry of Natural Waters. MartinusNijhoff/Dr. W. Junk Publishers, Dordrecht, Netherlands.
SAWYER, C.N., P.O. MCCARTY & G.F. PARKIN. 1994. Chemistry for
Environmental Engineering, 4th ed. McGraw-Hill, Inc., New York,
N.Y.
2. Apparatus
6. Calculation
A 50
B
where:
A estimated color of a diluted sample and
B mL sample taken for dilution.
b. The correct units for true color are CU. One CU is equivalent to one Hazen unit and to one Pt-Co unit. If samples are not
filtered, report data as Apparent CU. Report color results in
whole numbers and record as follows:
3. Reagents
CU
Record to Nearest
150
51100
101250
251500
1
5
10
20
Dissolve 1.246 g potassium chloroplatinate and 1.00 g crystallized cobaltous chloride in water with 100 mL conc HCl and
dilute to 1000 mL. This stock solution has a color of 500 color
units (CU). Platinum-cobalt standards of 500 CU are available
commercially, and are suitable for use as the primary standard.
Prepare standards having CU of 5, 10, 15, 20, 25, 30, 40, 50,
and 100 by diluting 1.0, 2.0, 3.0, 4.0, 6.0, 8.0, 10.0, and 20.0 mL
stock color standard with water in 100-mL volumetric flasks.
Transfer to nessler tubes for use as standards. Protect standards
against evaporation and contamination when not in use. Keep in
the dark when not in use, and keep only for 1 month.
7. Quality Control
5. Procedure
8. Reference
1. BLACK, A.P. & R.F. CHRISTMAN. 1963. Characteristics of colored
surface waters. J. Amer. Water Works Assoc. 55:753.
9. Bibliography
SAWYER, C.N., P.L. MCCARTY & G.F. PARKIN. 1994. Color. In Chemistry
for Environmental Engineering, 4th ed., Chap. 14. McGraw Hill,
New York, N.Y.
a. Principle: Color is determined spectrophotometrically at a wavelength between 450 and 465 nm, with platinum-cobalt solutions as
standards.13 True color of real samples and platinum-cobalt standards
follows Beers Law.
b. Application: The spectrophotometric platinum-cobalt method is
applicable to natural waters, potable waters, and wastewaters, both
domestic and industrial.
c. Interference: The primary interference is from the presence of
colloidal and suspended particles that absorb or scatter light at the
wavelength of the spectrophotometric method. While in Section 2120B
color measurements can be made without removal of particulate matter
as long as they are reported as Apparent CU, Method C requires
removal of particulate matter before color determination.
Light absorbance of organic matter depends on pH; however, the
variation in absorbance is small for the pH range of most waters.
Because color measurements are made for aesthetic reasons, preferably do not adjust sample pH as long as it is between 4 and 10. If
pH is adjusted, adjust to 7, and note. Further, pH can affect the
solubility of substances, which can then interfere with the color
measurement if particulate matter is formed.
d. Method detection level: The minimum detectable color depends
on the cell path length. Choose a cell size that provides an absorbance
within the range that results in good accuracy and linearity of response.
This range depends on the quality of the spectrophotometer. If a 50-mm
cell is used in the wavelength range of 450 to 465 nm, then an
absorbance of 0.005 yields a minimum detectable color of 1 CU. With
newer spectrophotometers, a method detection level of 2 CU can be
obtained with a path length of 25 mm. Dilute samples with high color
to fall within the range of the standard curve. Absorbance readings
should fall within the range of 0.005 to 0.8.
2. Apparatus
7. Quality Control
See 2120B.7.
3. Reagents
8. References
See 2120B.3.
4. Preparation of Standards
c. Interference: The primary interference is from the presence of colloidal and suspended particles that absorb or
scatter light.
3. Procedure
Wavelength
nm
424.4
435.5*
443.9
452.1
461.2*
474.0
531.2
544.3*
552.4
558.7
564.1*
568.9
573.2
577.4*
581.3
585.0
588.7*
592.4
596.0
599.6*
603.3
607.0
610.9*
615.0
619.4
624.2*
629.8
636.6
645.9*
663.0
465.9
489.5*
500.4
508.7
515.2*
520.6
525.4
529.8*
533.9
537.7
541.4*
544.9
548.4
551.8*
555.1
558.5
561.9*
565.3
568.9
572.5*
576.4
580.4
584.8*
589.6
594.8
600.8*
607.7
616.1
627.3*
647.4
414.1
422.2*
426.3
429.4
432.0*
434.3
436.5
438.6*
440.6
442.5
444.41
446.3
448.2
450.1
452.1
454.0
455.9*
457.9
459.9
462.0*
464.1
466.3
468.7*
471.4
474.3
477.7*
481.8
487.2
495.2*
511.2
4. Calculation
a. Tabulate transmittance values corresponding to wavelengths shown in Columns X, Y, and Z in Table 2120:I. Total
each transmittance column and multiply totals by the appropriate
factors (for 10 or 30 ordinates) shown at the bottom of the table,
to obtain tristimulus values X, Y, and Z. The tristimulus value Y
is percent luminance.
b. Calculate the trichromatic coefficients x and y from the
tristimulus values X, Y, and Z by the following equations:
0.033 33
0.100 00
X
X Y Z
Y
X Y Z
0.039 38
0.118 14
* Insert in each column the transmittance value (%) corresponding to the wavelength shown. Where limited accuracy is sufficient, use only the ordinates marked
with an asterisk.
FOR
Determine hue from the dominant-wavelength value, according to the ranges in Table 2120:II.
Wavelength Range
nm
Hue
400465
465482
482497
497530
530575
575580
580587
587598
598620
620700
400530c*
530c700*
Violet
Blue
Blue-green
Green
Greenish yellow
Yellow
Yellowish orange
Orange
Orange-red
Red
Blue-purple
Red-purple
5. Expression of Results
3. Procedure
2. Apparatus
See 2120B.7.
a. Spectrophotometer with narrow spectral band width (10 nm
or less), an effective operating range of 380 to 780 nm, and a
tungsten lamp light source. Instrument must be able to obtain
transmittance values at a multitude of wavelengths and calculate
tristimulus values X, Y, and Z1 to produce a final color result.*
Calibrate calculation algorithm software against platinum-cobalt
standard reference.
b. Spectrophotometer cells, 1 cm.
c. Filtering apparatus and filter: See 2120 B.2c.
5. Reference
1. AMERICAN SOCIETY FOR TESTING AND MATERIALS. 1995. Standard practice for computing the colors of objects by using the CIE system.
E308-95, ASTM Standards on Color and Appearance Measurement,
5th ed. American Soc. Testing & Materials, West Conshohocken, Pa.
6. Bibliography
HACH COMPANY. 1999. Hach DR/4000 Spectrophotometer Procedures
Manual, 9th ed. Hach Co., Loveland, Colo.
3. Procedure
See 2120B.7.
2. Apparatus
5. References
1. MCLAREN, K. 1970. The Adams-Nickerson colour-difference formula. J. Soc. Dyers Colorists 86:354.
2. ALLEN, W., W.B. PRESCOTT, R.E. DERBY, C.E. GARLAND, J.M. PERET &
M. SALTZMAN. 1973. Determination of color of water and wastewater
by means of ADMI color values. Proc. 28th Ind. Waste Conf., Purdue
Univ., Eng. Ext. Ser. No. 142:661.
6. Bibliography
HACH COMPANY. 1999. Hach DR/4000 Spectrophotometer Procedures
Manual, 9th ed. Hach Co., Loveland, Colo.