Oxaprozin

Molecular formula: C18H15NO3

Molecular weight: 293.3
CAS Registry No.: 21256-18-8

SAMPLE
Matrix: blood
Sample preparation: 1 mL Plasma + 50 \g ketoprofen + 200 (xL 1 M HCl + 4-5 mL ethyl
acetate, vortex for 1.5-2 min, centrifuge at 400 g for 10 min. Remove the organic layer
and evaporate it to dryness under reduced pressure at 40-50, reconstitute the residue in
200 |xL MeOH, inject a 20 jxL aliquot.
HPLCVARIABLES
Column: 100 X 5 10 |xm spherical C18 radial compression (Waters)
Mobile phase: MeCN-.water 45:55 containing 2.5 mL/L acetic acid
FIo1W rate: 1
Injection volume: 20
Detector: UV 280
CHROMATOGRAM
Retention time: 8
Internal standard: ketoprofen (5)
Limit of detection: 500 ng/mL
OTHER SUBSTANCES
Simultaneous: acetaminophen, indomethacin, phenylbutazone, salicylic acid
Noninterfering: ibuprofen, piroxicam
Interfering: fenoprofen, flurbiprofen
KEYWORDS
plasma; pharmacokinetics
REFERENCE
Matlis, R.; Greenblatt, D.J. Rapid high-performance liquid chromatographic analysis of oxaprozin, a
non-steroidal anti-inflammatory agent. J.Chromatogr., 1984, 310, 445-449

SAMPLE
Matrix: blood
Sample preparation: 500 fxL Plasma + 2.5 mL water + 1 mL 20-60 \xg/mL ketoprofen in
100 mM pH 4.8 acetate buffer, adjust to pH 2 with about 200 \xL 1 M HCl, add 8 mL
ether, shake mechanically for 10 min, centrifuge at 2000 rpm for 5 min. Remove 7 mL of
the organic layer and evaporate it to dryness under reduced pressure at room temperature, reconstitute the residue in 500 jxL mobile phase, inject a 10 jxL aliquot.
HPLCVARIABLES
Column: 300 X 4.6 10 |xm Chromegabond C18 (E.S. Industries)
Mobile phase: MeCN: buffer 62:38 (Buffer was 50 mM NaH2PO4 adjusted to pH 3.9 with
phosphoric acid.)
Flow rate: 1.5
Injection volume: 10
Detector: UV 290

CHROMATOGRAM
Retention time: 5.5
Internal standard: ketoprofen (3.5)
Limit of detection: 500 ng/mL
KEYWORDS
plasma; human; pharmacokinetics
REFERENCE
McHugh, S.L.; Kirkman, S.K.; Knowles, J.A. Macro- and micromethods for high-performance liquid
chromatographic analysis of oxaprozin in plasma. J.Pharm.ScL, 1980, 69, 794796

SAMPLE
Matrix: blood, urine
Sample preparation: 50 (xL Plasma or urine + 50 |xL 100 mM pH 7 phosphate buffer +
50 |xL 5-20 |xg/mL ketoprofen in 100 mM pH 7 phosphate buffer + 200 |xL ether, vortex
for 5 s, centrifuge, repeat extraction twice. Combine the organic layers and evaporate
them to dryness under a stream of nitrogen, reconstitute the residue in 50 |xL mobile
phase, inject a 20 jxL aliquot.
HPLCVARIABLES
Guard column: 50 X 2.1 37-45 \xm Co:Pell ODS
Column: 300 X 4.6 10 |xm Chromegabond C18 (E.S. Industries)
Mobile phase: MeCN.buffer 60:40 (Buffer was 50 mM NaH2PO4 adjusted to pH 3.9 with
phosphoric acid.)
Flow rate: 1.5
Injection volume: 20
Detector: UV 280
CHROMATOGRAM
Retention time: 4.5
Internal standard: ketoprofen (3.5)
Limit of detection: 100 ng/mL
OTHER SUBSTANCES
Extracted: metabolites
KEYWORDS
plasma; rat; pharmacokinetics
REFERENCE
McHugh, S.L.; Kirkman, S.K.; Knowles, J.A. Macro- and micromethods for high-performance liquid
chromatographic analysis of oxaprozin in plasma. J.Pharm.Sci., 1980, 69, 794-796

SAMPLE
Matrix: bulk
Sample preparation: Prepare a 400 |xg/mL solution in mobile phase, inject a 10 JXL
aliquot.
HPLCVARIABLES
Column: 300 X 3.9 ixBondapak C18
Mobile phase: MeCN: MeOH: buffer 25:25:50, adjusted to pH 4.2 with 85% phosphoric
acid (Buffer was 10 mM KH2PO4 containing 5 mM sodium 1-decanesulfonate.)
Flow rate: 1

Injection volume: 10
Detector: UV 254
CHROMATOGRAM
Retention time: 19.8
Limit of detection: 54 ng/mL
OTHER SUBSTANCES
Simultaneous: impurities
KEYWORDS
stability-indicating
REFERENCE
Ibrahim, RB. Quantitative determination of oxaprozin and several of its related compounds by highperformance reversed-phase liquid chromatography J.Liq.Chromatogr., 1995, 18, 2621-2633

SAMPLE
Matrix: solutions
HPLCVARIABLES
Column: 100 X 2.1 C-8 (Brownlee)
Mobile phase: MeCN: buffer 28:72 (Buffer was 500 mM pH 4.6 sodium phosphate buffer
containing 5 mM tetrabutylammonium phosphate.)
Flow rate: 0.5
Detector: UV 254
CHROMATOGRAM
Retention time: 15.71
OTHER SUBSTANCES
Simultaneous: metabolites, glucuronides
REFERENCE
Wells, D.S.; Janssen, RW.; Ruelius, H.W Interactions between oxaprozin glucuronide and human serum
albumin. Xenobiotica, 1987, 17, 1437-1449