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PLANT HORMONES
Biochemistry and
Metabolism
Plants Hormones :
Means intercellular comunication within plants is mediated by
the action of chemical messengers
(i) AUXIN
Natural auxins :
Indole-3-acetic acid (IAA)
4-chloroindole-3-acetic asid
Phenylacetic acid
Indole-3-butyric acid (IBA)
Synthetic auxins:
Naphthaleneacetic acid (NAA)
2-Methoxy-3,6-dichloro-benzoic acid (dicamba)
2,4-Dichlorophenoxyacetic acid (2,4-D)
2,4,5-Trichlorophenoxy-acetic acid (2,4,5-T)
Amount of IAA present depend on a number of
factors :
Wents discovery :
Growth promoting substance from excised coleoptile
tips would diffuse into agar block
Blocks could the used to restore growth in decapitated
coleoptile
If agar block containing auxin was placed on one side
of coleoptile stump coleoptile bent away from the
side containing the block
Curvature occurred because:
i. the increase in auxin on one side stimulated cell
elongation; and
ii. decrease in auxin on the other side caused a
descrease in the growth rate
Refer to fig 16.1 page 400 (Taiz /Zieger)- Plant
Physiology
Transport of auxins
Depend on the developmental stage of
theorgan/tissue of plant
Transport of hormone into or out of tissue/organ
influence level of active hormone within
tissue/organ
Hormone (Auxin) transport of plant is the polarity
of movement
Polar transport expressed as movement in one
direction
This indicate that polarity is not driven by external
forces (gravity etc.) but the property of cells
themselves
14
C-IAA
14C-IAA
A
B
Receiver
block
B
A
A
B
B
A
14C-IAA
14C-IAA
Normal
orientation
Inverted
sections
1. Cell elongation/enlargement
Stimulate cell elongation in excised tissue
(coleoptile)
Auxin concentration response curve show
increasing response with increasing
concentration of auxin an optimum
concentration is reached
Concentration exceeding the optimum
rduced growth
Concentration high growth inhibited
GIBBERELLINS
Gibberellins members of terpenoids
Gibberellins; assigned as GA
most important in higher plants
GA3 extracted from fungal culture
3 principal sites of gibberellin biosynthesis :
(i) Developing seeds (developing endosperm,
cotyledon, scutellum)
(ii) Developing fruits
(iii) Young leaves of developing apical bud and
elongating
shoots
(iv) Apical region of root
PHYSIOLOGICAL ACTION OF
GIBERELLINS
1.Stimulate hyperelongation of
intact stems
Occurs especially in dwarf and rosette plants
Promote elongation in intact plants rather than
excised tissues
Study on dwarf mutants of Oryza sativa, Zea
mays, Phaseolus vulgaris
These mutants exhibit the normal phenotype
when treated with GA3
GA treated enhanced internode elongation
2. Seed germination
Cereal grain seeds like Hordeum vulgare
consists of embryo and non embryonic
regions
Embryo region synthesized GA and induced
release of -amylase to hydrolyze endosperm
(starch)
Non embryonic region if treated with GA will
stimulate to produce -amylase at high
concentration
-amylase
(inactive)
aleurone
endosperm
-amylase
(active)
starch
-amylase
protease
glucose
scutellum
coleoptile
GA
plumule
radical
embryo
4. Flowering
Flowering is induced by gibberellins
Eg. Pharbitis nil, Chenopodium rubrum will
flower immediately
Many perennial plants capable of flowering
after pass through a juvenile phase
Gibberellins overcome juvenile phase in many
conifers and stimulate precocious flowering
Gibberellins promote maleness in unisexual
flowers while auxin promote femaleness
5. Inhibition of gibberellins
biosynthesis
Growth of stem can be inhibited or reduced by synthetic
chemical that block gibberellin biosynthesis i.e growth
retardant or anti gibberellins (AMO-1618, cycocel,
Phosphan-D, ancymidol)
These compound has commercial application in production
of ornamental plants
Effects:
To reduce stem elongation
Results in shorter and compact plants with darker
green foliage
Spraying the plant (Wheat) with antigibberellin produce a
shorter, stiffer stem and preventing lodging
CYTOKININS
Cytokinin- adenine derivatives
Kinetin- first compound found with cytokinin
activity
Synthetic cytokinin prepared by heating DNA
Zeatin- first natural cytokinin discovered and
most widespread
Isopentenyl adenine (iP)
Dihydrozeatin (diHZ) : leass active than
zeatin
Benzyl adenine (BAP)
Crown gall
2. Organogenesis
Cytokinin and auxin stimulate organogenesis:
organ formation
Development of shoot and root
Cell culture growth required cytokinin and
auxin
High Cytokinin/auxin ration stimulate root
formation
Low cytokinin/auxin ration stimulate shoot
formation
3. Senescence
Mature leaves and fruits express senescence
Senescence: breakdown of protein, nucleic acids,
other macromolecules, loss of chlorophyll,
accumulation of amino acids
Cytokinins will delay senescence while ethylene
promote senescence
Cytokinins direct nutrient mobilization and retention
by stimulating metabolism in the area of application
Creates a new sink: area that attract metabolites
from region of application
senescence
kinetin
radioactive
C
A
Radioactive spreads
into vascular tissue
for export through
petiole
B
Radioactive
accumulates in the
area treated with
kinetin
Radioactive
retain near poin
of application
Abscisic Acid
2. Bud/seed dormancy
Woody plants in temperate zone
ABA concentration maximum in early winter and
low end of winter
ABA prevents bud development and seed
germination
ETHYLENE
Simple hydrocarbon gaseous : H2C=CH2
Not required for normal vegetative growth
Synthesized primarily in response to stress
Produced in large amounts by tissues
undergoing senescence or ripening
Occurs in all plant organs
Fruit Ripening
Stimulate fruit ripening: banana, apple avocado
etc..
Ethylene is autocatalytic
release of ethylene gas by ripening fruits
will in turn stimulate premature climacteric
and ethylene production by other fruits stored
near
Number of qualitative metabolic changes are
initiated in fruit
Brassinosteroids
Steroid hormones
Chemical structure similar to animal steroid
hormones
Brassinolides most biologically actve
Functions: brassinosteroids promote stem
elongation in mutant plants, shoot elongation
and ethylene production
Inhibits root growth and development