Hema Star II

Technical information

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Summary
SUMMARY

ELECTRICITY SUPPLY

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GENERAL REMARKS
FIRST POWER SUPPLY
SECOND POWER SUPPLY
SCREEN

DESCRIPTION OF ELECTRONIC BOARDS

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SYSTEM BOARD
PREAMPLIFIER BOARD L10-CI-PRA20
PROCESSING BOARD L1O-PR-FIL3O
MECHANICAL / FLUIDIC MANAGEMENT BOARD L10-CI-DIL30
INTERFACE BOARD L1O-CI-INT-AA

SEQUENCING OF MAIN FLUIDIC CYCLES

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DILUTION SEQUENCING
COUNTING CYCLE
RINSING AND DRAINAGE AT THE END OF COUNTING CYCLE (DILUTER)
RINSING AND DRAINAGE AT THE END OF CYCLE (FLUIDIC)
SEQUENCING FIRST RINSING CYCLE
SEQUENCING OF RINSING CYCLE EXCEPT THE FIRST
SEQUENCING OF RINSING CYCLES AFTER 5 MINUTES

LIST OF ELECTRONIC SCHEMES

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LIST OF FLUIDIC SCHEMES

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Electricity supply
1. General remarks
The device works on 10w voltage power from two sources in ventilated casing. The
main supply element is Iocated at the back of the device preventing any risk of
electrocution.
Important:
These partitioned power supplies do not have any accessible fuses. Contact your
technical support.
N.B: the presence of the different voltages displayed by leds on the interface
board (appendix E3).
2. First power supply
PC type power supply giving all boards the following voltages:
+12V
-12V
+5V
Power supply linked to the floppy disk driver and the system board, then to all other
boards via the expansion slots and cable layers.
It works on 50 or 60 Hz and there is a switch between the two EEC jacks for
changing to 115 or 230V.
3. Second power supply
Works on 30 VI 2 A, feeding the interface board.
Accepts from 47 to 440 Hz and 2 voltage ranges, switched over by internal strap or
automatic according to the version:
- 95 to 132V
-187 to 265V
4. Screen
Works on fixed voltage 220 V, 50 to 60 Hz. On request, it may be supplied with
different power supply voltages.

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Description of electronic boards

Refer to block diagram : appendix E1.
1. System board
Board of 80386 or 80486 type. The program, written in an advanced language,
loaded in the core memory after power on.
The dialog with the peripheral boards L10-CI-FIL-30 and L10-CI-DIL30 is in mode 1/0
by reciprocal interruption.
2. Preamplifier board L10-CI-PRA20
It polarizes the two counting probes and amplifies the signals. The hemoglobin
determination system is on the preamplifier board.
3. Processing board L1O-PR-FIL3O
Made up of three structures with independent Z80 microprocessors. Each structure
has a specific task :
counting of red corpuscles
platelets
white corpuscles / hemoglobin.
The different signals are sorted out on peak width and height.
4. Mechanical / Fluidic management board L10-CI-DIL30
Two Z80 structures deal with the mechanical and fluidic management of the
device. They control all the fluidic functions and all the motors following the
program of the different cycles.
5. Interface board L1O-CI-INT-AA
Power interface board which is linked to the management board by a 60 lead
cable layer.
Diluter Inputs / Outputs :
needle mechanism
liquid syringe
from electrovalve EV1 to EV3
start button
red and green leds to start the system up.
Fluidics Inputs / Outputs :
volumetric counting sensor and drainage optic sensor
liquid micro-pump
from electrovalve EV4 to EVI 2
air syringe

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Sequencing of main fluidic cycles

1. Dilution sequencing

PHASE

SYRINGE
BLOOD
Sample 32 l
Amount 11 ml
SAMPLE
EXT. RINSING 3 ml ext. needle
OF NEEDLE
FIRST
DILUTION

NEEDLE
Total ascent
Descent 40 mm

HOLDER

Init rinsing

EV1 EV2 EV3

Ascent 15 mm
Rinsing WBC
Descent 15 mm

2 ml ext. needle

8
8
8
8

Ascent 5 mm

2 ml int. needle
1.5 ml ext. needle
1.5 ml int. needle to init
DROP
EXTRACTOR

Ascent 5 mm

8
8
8
8

Descent 15 mm
Ascent 15 mm
Flush elimination

SAMPLE IN
DILUTION 1

Descent 23 mm
Sample 43.7 l
Ascent 29 mm

Amount 10 ml
EXT. RINSING
1.5 ml ext. needle
OF NEEDLE

WBC rinsing

SECOND
DILUTION

Rinsing RBC

Descent 9 mm

REQUEST TRANSFER CHAMBER WBC + LYSIS

8 8
8 8 8

6.5 ml ext. needle

1.5 ml int. needle
RINSING
LIQUID IN
CHAMBER
WC

Ascent 5 mm
Amount 7 ml
7 ml ext. needle

RBC WBC

REQUEST TRANSFER CHAMBER RBC

RINSING
Amount 7 ml
6 ml ext. needle
LIQUID IN
CHAMBER RC 1 ml int. needle

WBC RBC

8 8
8 8

REQUEST COUNTING CYCLE

POSITION
AWAIT OF
COUNTING

To switch
RBC rinsing
Descent 20 mm

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2. Counting cycle
PHASE
FIRST COUNTING CHART

SECOND COUNTING CHART

THIRD COUNTING CHART

FOURTH COUNTING CHART

ACTION
Pressure lowering with air syringe
Injection of a bubble into the capillary
Bubble passes in front of the start sensor
Bubble passes in front of the end sensor
Control of counting time
Transfer of results WC, RC, PLT to PC
Counting of duration determined by first chart
Transfer of results WBC, RBC, PLT to PC
Pressure lowering with air syringe
Injection of a bubble into the capillary
Bubble passes in front of the start sensor
Bubble passes in front of the end sensor
Control of counting time
Transfer of results WC, RC, PLT to PC
Counting of duration determined by third chart
Hemoglobin measure
Transfer of results WBC, RBC, PLT to PC

3. Rinsing and drainage at the end of counting cycle (diluter)

PHASE
AFTER THE
END OF
COUNTING

SYRINGE

NEEDLE

HOLDER
To slide
Slide return

EV1 EV2 EV3

Backlash elimination
Total ascent
Descent 75 mm

Complete return

READY FOR ANOTHER CYCLE

4. Rinsing and drainage at the end of cycle (fluidic)
CHAMBER DIL. WBC

CHAMBER DIL. RBC

CHAMBER WBC
Drainage

CHAMBER RBC

Transfer
CHAMBER READY
Drainage
Drainage
Transfer
CHAMBER READY
Drainage
Drainage control
Drainage control

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5. Sequencing first rinsing cycle

PHASE

SYRINGE

NEEDLE

HOLDER

EV1 EV2 EV3

REQUEST CAPILLARY RINSING

REQUEST DRAINAGE OF CHAMBERS WBC AND RBC

Ascent 50 mm

1st DISTRIBUTION
TO RINSING
CHAMBER

Init rinsing

Descent 50 mm
Amount 9 ml
4 ml ext. needle

8 8

REQUEST DRAINAGE OF NEEDLE RINSING CHAMBER

8 8

4 ml int. needle
REQUEST DRAINAGE OF NEEDLE RINSING CHAMBER

2nd DISTRIBUTION TO Amount 9 ml

RINSING CHAMBER 4 ml ext. needle

8 8

REQUEST DRAINAGE OF NEEDLE RINSING CHAMBER

8 8 8

4 ml int. needle
REQUEST DRAINAGE OF RINSING CHAMBER
REQUEST DRAINAGE OF COUNTING CHAMBER WBC

1st DISTRIBUTION
Amount 8 ml
TO CHAMBER WBC

Ascent 30 mm

Rinsing WBC

Descent 30 mm

8
8 8

5 ml ext. needle
2 ml int. needle
REQUEST TRANSFER CHAMBER WBC
REQUEST DRAINAGE OF COUNTING CHAMBER WBC

2nd DISTRIBUTION
Amount 8 ml
TO CHAMBER WBC 5 ml ext. needle
2 ml int. needle

8 8
8 8

Ascent 30 mm
Descent 30 mm
1st DISTRIBUTION
TO CHAMBER RBC

WBC RBC

Amount 9 ml
6 ml ext. needle
2 ml int. needle

8 8
8 8

REQUEST TRANSFER CHAMBER RBC

REQUEST DRAINAGE OF COUNTING CHAMBER RBC

2nd DISTRIBUTION
TO CHAMBER RBC

Amount 9 ml
6 ml ext. needle
2 ml int. needle

8 8
8 8

REQUEST TRANSFER CHAMBER RBC

REQUEST DRAINAGE OF COUNTING CHAMBER RBC

3rd DISTRIBUTION
Amount 9 ml
TO CHAMBER WBC
REQUEST TRANSFER CHAMBER WBC + LYSIS

6 ml ext. needle
2 ml int. needle

8 8 8
8 8 8

REQUEST TRANSFER CHAMBER RBC

REQUEST COUNTING CYCLE

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6. Sequencing of rinsing cycle except the first

PHASE

SYRINGE

NEEDLE

HOLDER

EV1 EV2 EV3

REQUEST CAPILLARY RINSING

REQUEST DRAINAGE OF CHAMBERS WBC AND RBC

1st DISTRIBUTION
TO RINSING
CHAMBER

Amount 5 ml

Total ascent
Init rinsing
Descent 50 mm

8 8
8 8

2 ml ext. needle
2 ml ext. needle
REQUEST DRAINAGE OF NEEDLE RINSING CHAMBER

Ascent 30 mm

DISTRIBUTION TO
CHAMBER WBC

Descent 30 mm

Rinsing WBC

Amount 9 ml
6 ml ext. needle
2 ml int. needle
DISTRIBUTION TO
CHAMBER RBC

8 8
8 8
Ascent 30 mm

Amount 9 ml

Descent 30 mm

WBC RBC

REQUEST TRANSFER CHAMBER WBC

6 ml ext. needle
2 ml int. needle

8 8 8
8 8 8

REQUEST TRANSFER CHAMBER RBC

REQUEST COUNTING CYCLE

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7. Sequencing of rinsing cycles after 5 minutes

PHASE

SYRINGE

NEEDLE

HOLDER

EV1 EV2 EV3

REQUEST CAPILLARY RINSING

REQUEST DRAINAGE OF CHAMBERS WBC AND RBC

Total ascent

1st DISTRIBUTION
TO CHAMBER WBC

Descent 50 mm

Amount 8 ml

Init WBC

8 8
8 8

5 ml ext. needle
2 ml ext. needle
REQUEST TRANSFER CHAMBER WBC

2nd DISTRIBUTION
Amount 8 ml
TO CHAMBER WBC
REQUEST DRAINAGE OF CHAMBER WBC

8 8
8 8

5 ml ext. needle
2 ml int. needle
DISTRIBUTION TO
CHAMBER RBC

Amount 8 ml
Ascent 30 mm
Descent 30 mm

WBC RBC

REQUEST TRANSFER CHAMBER WBC

5 ml ext. needle
2 ml int. needle

8 8 8
8 8 8

REQUEST TRANSFER CHAMBER RBC

REQUEST CAPILLARY RINSING
REQUEST CAPILLARY RINSING
MECHANICAL INITIALIZATIONS

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List of electronic schemes

Appendix D1 :
Appendix D2 :
Appendix D21 :
Appendix D3 :
Appendix D31 :
Appendix D4 :
Appendix D41 :

Preamplifier board L10-CI-PRA20

A/D conversion board L10-ANA20
Component location on conversion board
Processing board L10-CI-FIL30
Component location on processing board
Mechanical / Fluidic management board L10-CI-DIL30
Component location on mechanical / fluidic management
board
Appendix D5 :
Interface board L10-CI-INT-AA
Appendix D51 : Component location on interface board

Appendix E1 :
Appendix E22 :
Appendix E3 :
Appendix E44 :

Machine block diagram

Trimmer location on preamplifier board
Trimmer location on interface board
Trimmer location on A/D conversion board

List of fluidic schemes

Appendix F1 :
Appendix F2 :
Appendix F21 :
Appendix F22 :
Appendix F23 :
Appendix F24 :
Appendix F25 :
Appendix F26 :
Appendix F3 :
Appendix F4 :

Fluidic cabling
Fluidic skeleton diagram
WBC dilution transfer
RBC dilution transfer
Volumetric sensor rinsing (detergent circuit)
Counting
Drainage of counting chambers
Diluter sequences
Dismounting of ruby apertures
Air and liquid syringe greasing

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Appendix D1
PREAMPLIFIER BOARD L10-CI-PRA20

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Appendix D2
A/D CONVERSION BOARD L10-ANA20

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Appendix D21
COMPONENT LOCATION ON CONVERSION BOARD

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Appendix D3
PROCESSING BOARD L10-CI-FIL30

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Appendix D31
COMPONENT LOCATION ON PROCESSING BOARD

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Appendix D4
MECHANICAL / FLUIDIC MANAGEMENT BOARD L10-CI-DIL30

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Appendix D41
COMPONENT LOCATION ON MECHANICAL / FLUIDIC MANAGEMENT BOARD

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Appendix D5
INTERFACE BOARD L10-CI-INT-AA

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Appendix D51
COMPONENT LOCATION ON INTERFACE BOARD

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Appendix E1
MACHINE BLOCK DIAGRAM

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Appendix E22
TRIMMER LOCATION ON PREAMPLIFIER BOARD

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Appendix E3
TRIMMER LOCATION ON INTERFACE BOARD

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Appendix E44
TRIMMER LOCATION ON A/D CONVERSION BOARD

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Appendix F1
FLUIDIC CABLING

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Appendix F2
FLUIDIC SKELETON DIAGRAM

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Appendix F21
WBC DILUTION TRANSFER

Tubes concerns

EV contrles

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Appendix F22
RBC DILUTION TRANSFER

Tubes concerns

EV contrles

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Appendix F23
VOLUMETRIC SENSOR RINSING (DETERGENT CIRCUIT)

Tubes concerns

EV contrles

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Appendix F24
COUNTING

Tubes concerns

EV contrles

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Appendix F25
DRAINAGE OF COUNTING CHAMBERS

Tubes concerns

EV contrles

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Appendix F26
DILUTER SEQUENCES

Blood or 2nd
dilution extraction

Needle external distribution

Diluent loading

Needle internal distribution

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Appendix F3
DISMOUNTING OF RUBY APERTURES

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Appendix F4
AIR AND LIQUID SYRINGE GREASING

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