.

. . / / .
. . / / .
. . / / .

129 ,
, , , 18
Trichophyton ) (%51.57 ) Microsporum (27.36%
) (%64.21 ) .(%35.79 )
(%31.57 , )(%38.95
.
18
) (%100
ketoconazole ) Griseofulvin, Miconazole .(Nystatin

Summary
A total of, 129 Samples were collected to isolate and identify the dermatophytes
and These samples included skin scrapping, hair fragments, nail clippings from
human were examined directly by wet and stained preparation then cultured using the
selective media for growing and isolation of fungi.
The results revealed that more.
In respect with human samples, there were 18 fungal species isolated and
identified and the genus Trichophyton was the more frequent (51.57%), than
Microsporum (27.36%) and the females were the more infected (64.21%) other than
males (35.79%). In addition to that, the nails region were the more infected (31.57%)
when compared with other body regions, and the ages < 10 years were more infected
(38.95%) than other ages.
The antifungal susceptibility test for 18 fungal isolated against some antifungal
drugs using well diffusion method was done,
showed a high sensitivity against

in vitro, where all the isolates

ketoconazole (100%) than other

antifungal (Nystatin, Miconazole, and Griseofulvin).

 .
,
Normal flora
) (Candida ) .(1
).(2
) (Mycoses
) .(2

) . (3 )
(Dermatophytes
,
) ,(4) (Keratinophilic fungi


)(5



) (

).(6


) (Narro spectrum )Broad
(spectrum
) .(7
) .(8

E. floccosum M. canis

M. gypseum T. mentagrophytes T. rubrum T. tonsurans

Clotrimazole Itraconazole ,Terbinafine
. Terbinafine




:


.

:
.
129) (
. 74
55 ,

) (.

.
)(SDA
65 1000 0.05
Chloramphenicol
0,5 / Cycloheximide
, %30

. ). (9
)(ESDA
20 10 20 , 1000
0.05 Chloramphenicol
.
10 . ). (10

 .
) (
KOH %10

.
28
,
) (SDA ) (Slant
25 )(6-4

) .(Lactophenol cotton blue
.(11121314151617) :

Drug Sensitivity test for fungi

) (Agar-well- diffusion method
)Miconazole,
. (18) (Griseofulvin, Ketoconazole, Nystatin

Fungal inoculation
) (10 )(SDA
) (Vail 5
) (Normal saline . 105

Antifungal preparation
) (10000/
) (10 5 ) Dimethylsufloxide (DMSO %100
) (Vail ) (50 )Vortex
,(mixer 10000/ 1000

/ 10 ) (DMSO
%100 1 10000/ ,
30 , :
.1 0.2 ) (Micropipette )
(ESDA ) (Spreader ) (L
. 30 .
.2 5 ).(19
.3 0.1 )
,(Micropipette 28 )(5-2 , )
(Inhibition zone ) . ( 20

Statistical analysis
) Qi-square (X2 ) (21
.0.01

Results :
129 , 74 55 ,
95 18 ) ,(1 Trichophyton
Microsporum ) ,(2
) ,(0.01 (%64.21) 61
) (%35.79) 34 (2 ) ( Tinea unguium
) (%31.57 ) (24.21%) ( Tinea pedis
) Tinea capitis ) ) (20% )) (0.01 3 ,(4
) (0.01
20-11 40-21 ). (5
) ( 6
) ( . Ketoconazole
Miconazole Microsporum
Trichophyton ) (16,17 ) (13,15
Aspergillus ) (0,5 .

: :(1)
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
`17
18

Aspergillus flavus Link ex Gray

A. fumigatus Fresenius
Candida albicans (Robin) Berkhout
C. parapsilosis (Ashford) Lengeron and Talice
C. tropicalis (Castell) Berk
Chrysosporium keratinophilium (Frey) Cormichael
C. tropicum Carmichael
Epidermophyton floccosum (Harz) Langeron and Milocheritch
Microsporum canis Bodin
M. gypseum (Bodin) Guiart and Grigorakis
M. nanum Fuents Guiart and Grigorakis
M. persicolor (Sabouraud) Guiart and Grigorakis
Trichophyton kanei Summer bell
T. mentagrophytes (Robin)Blanchard
T. mentagrophytes var mentagrophytes (Robin)Blanchard
T. rubrum (Castellani) Sabouraud
T. verrucosum Bodin
T. violaceum Bodin

. :(2)

1
1
2
4

1
0
2
2

0
1
0
2

Aspergillus flavus
A. fumigatus
Candida albicans
C. parapsilosis

2
2
1
7
7
10
6
3
2
3
3
19
11
11
95

2
2
0
5
4
6
5
1
2
0
3
11
7
8
61

0
C. tropicalis
0
Chrysosporium keratinophilium
1
C. tropicum
2
Epidermophyton floccosum
3
Microsporum canis
4
M. gypseum
1
M. nanum
2
M. persicolor
0 Trichophyton kanei
3
T. mentagrophytes
0 T. mentagrophytes var mentagrophytes
8
T. rubrum
4
T. verrucosum
3
T. violaceum
34

35.79%

64.21%

7.840= (2)
1 =
0.01

 ) :(3 .

Aspergillus flavus
A. fumigatus
Candida albicans
C. parapsilosis
C. tropicalis
Chrysosporium keratinophilium
C. tropicum

1/

Epidermophyton floccosum

2/

1/

1/
2/

1/
1/
1/
1/
1/
2/

Microsporum canis
M. gypseum
M. nanum

1/1/
2/
2/
2/
2/
1/
2/
1/
2/
1/
1/
4/
1/
2/
1/

1/
2/
M. persicolor

2/

Trichophyton kanei
T. mentagrophytes

2/
1/
2/

T. mentagrophytes var. mentagrophytes

T. rubrum

T. verrucosum
T.violaceum

1/

2/
1/
3/
2/
1/
2/
1/
2/
1/

1/
2/
4/
3/
2/
2/
2/
2/
3/
2/
3/
3/

 ) :(4

30
23
19
14
5
2
1
1
95

%
31.57
24.21
20
14.73
5.26
2.11
1.06
1.06
100

) , 80.48= (2 = , 7 0.01

. :(5)

21-35

11-20

4-10

21-40

11-20

4-10

1
1
1

1
1
2

1
1

1
1
2
2
1

2
2
3
2

2
1
1
1
1
1

2
2
1

1
1

1
2

5
3
3
3
1
2
4
1
2
3
3
1
18
19 24
10

2
1

Aspergillus flavus
A. fumigatus
Candida albicans
C. parapsilosis
C. tropicalis
Chrysosporium keratinophilium
C. tropicum
Epidermophyton floccosum
Microsporum canis
M. gypseum
M. nanum
M. persicolor
Trichophyton kanei
T. mentagrophytes
T. mentagrophytes var. mentagrophytes

2
3
2
1
1
1
11
13
,5 =

T. rubrum
T. verrucosum
T. violaceum

, 11.240= (2)
.0.01

 ) :(6

)(
Miconazol Ketoconazol Nystati

Griseofulvi

Microsporum nanum

n
6

e
17

e
18

Trichophyton verrucosum

15

13

0 Trichophyton kanei
4 Trichophyton rubrum
0 Aspergillus fumigatus

16
14
5

15
14
0

7
9
3

3
4
5

. 0.01 )13,232= (2

 :
Trichophyton
Microsporum T. rubrum %14.72
) . ( 22 ) (23
) . (Tinea unguium
Lipase
Phospholipase . T.
verrucosum T. violaceum %8.52
T. verrucosum ) (Zoophilic
) (3 T. violaceum
) (Anthropophilic
) (Tinea ) ,(Tinea unguium ) ,(Tinea corporis )Tinea
(capitis ) .(3) (Tinea barbae
Candida C. parapsilosis
) ,(1 ) ( 24
)
(Fungemia ,C. parapsilosis ( ( 24
) .(25

, ) ( 24 T. rubrum T.
mentagrophytes var. erinacei ) (Tinea
) (Tinea capitis
) (Tinea pedis
) .(26
,
) (10



.


)(
,



). ( 6 ) (5

Ketoconazole ) ( 18
,
) (Tinea ),(18) (Tinea capitis
Nystatin ,
,
) (Local candidiosis .

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