UNL44 Biology Microscope Lab

Student Name: 4x, 10x, 40x and 100x.

(Please type in the grey boxes. Instructions for submitting this document will be
given at the end of the online lab)
Part I Name that part:
Look at the image on the page and type the name of each part in the
corresponding area below
(please refer to previous Name That Part Slide for naming)
A) Eyepeices
J) abbe condenser locking screw
B) Diopter adjustment
K) abbe condenser 1.25 na
C) Viewing head
L) iris diaphragm lever
D) mark on the side indexing
dioptor reading

M) coarse focus knob

E) revolving nosepiece

N) fine focus knob

F) objective lenses

O) iluminator condenser

G) arm of microscope stand

P) knob controlling x and y

movement of mechanical stage

H) specimen holder
Q) base
I) stage
R) light intensity control knob

Microscope Lab

Page 1

Part 2 Total Microscope Magnification

Read the questions online and type your answers below.
Question 1 Total magnification = 50
Question 2 Total magnification = 600
Question 3 Total magnification = 100
Question 4 Objective Lens = 40 Ocular Lens = 10
Question 5 Objective Lens = 60 Ocular Lens = 25
Question 6 Objective Lens = 60 Ocular Lens = 10
Question 7 Objective Lens = 20 Ocular Lens = 25
Question 8 Objective Lens = 20 Ocular Lens = 10
Question 9 Objective Lens = Ocular Lens =
Part 3 Pre Lab Questions
Question 1 coarse adjustment knob
Question 2 low, medium and high have different ways to distinguish them;
one being the length, the highest power which is 40X is the longest in
size.
Question 3
Question 4 use to fine-tune the focus of the specimen after using the
coarse adjustment knob, makes it more clear
Question 5 used for focusing the specimen, just so you can see it
Question 6 Coarse adjustment is used to focus the image on the
microscope under low or medium power by using larger motions of the
lens.
Fine adjustment is used to focus the image on the microscope of only high
power by using very tiny motions of the lens.
Question 7 You would use 100X.
Question 8 diaphragm Regulates the amount of light entering the
microscope.

Part 4 Post Lab Questions

Question 1 oarse adjustment- to bring the specimen into focus from all the
other mess in the slide
Question 2 to bring a part/layer of the specimen into focus
Question 3 as the power of the objective lens increases, the depth of field
decreases
Question 4
Question 5
Question 6 High powered allows you to "zoom in" closer and see more
detail, and a low power allows a larger field of view with less detail.
Question 7 4X, 10X, 40X,100X
Question 8
Question 9 he high power is zoomed in more on the slide, so it has a
smaller viewing area. With the Low Power, you can see a large area, and
find you specimen, and then zoom in on it with the High Power Lens.
Question 10 Each time light passes through a lens, it inverts. Two lenses in
a row results in two inversions, and a net "normal image." Compound light
microscopes, the ones you likely use in Lab, have multiple lenses in the
path of light, resulting in the inversion phenomenon.