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Research Article
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1.
Introduction
Gauchan et al
NAA(mg/l)
1.0
2.0
1.0
2.0
3.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
1.0
1.5
1.0
1.5
0.0
0.0
0.0
0.0
0.0
IBA(mg/l)
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.5
1.0
1.5
2.0
2.5
BAP: Benzyl amino purine; NAA: Naphthalene acetic acid; KN: Kinetin; 2, 4- D: 2, 4-Dichlorophenoxy acetic acid;
IBA: Indole-3-butyric acid.
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Gauchan et al
Ethanol (70%)
Exposure Time
HgCl2 (0.1%)
Exposure Time
Response of
Explants
Contamination
1 minute
1 minute
1 minute
1 minute
1minute
6 minutes
5 minutes
4 minutes
3 minutes
2 minutes
6 minutes
5 minutes
4 minutes
3 minutes
2 minutes
Brown (dead)
Brown (dead)
Brown (dead)
Green (live)
Green (live)
Brown (dead)
Brown (dead)
Brown (dead)
Green (live)
Green (live)
+
+
+
S. No.
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
21
22
23
24
25
Callus morphology
Slight whitish green
Light green
Light green
Light yellowish green
Light green
Light green
Yellowish Green
Light yellowish green
Yellowish Green
Light brown
Slight whitish Green
Light green
Light yellowish green
Light green
Light green
Light yellowish green
Slight whitish green
Light green
Light yellowish green
Slight whitish green
Yellowish green
Light green
Yellowish green
Light green
Light brown
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Gauchan et al
Figure 1: Proliferation of callus in media supplemented with different phytohormones in different concentration (A: Light yellowish
green callus; B: Light greenish yellow callus; C: Greenish yellow callus; D: Brownish yellow callus).
S. No.
1
2
Sample
Green leaf
Callus
Flavonoids
+++
++
Glycosides
+
+
Phenol
+++
++
Lignin Sterols
_
++
_
+
Tannins
+++
++
Saponins
+++
++
+ Positive notification of the presence of tested group, +++ Distinct notification, ++ Clear notification, - Absence
Table 4: Phytochemical Screening
4.
Conclusion
44
Gauchan et al
6)
7)
8)
Acknowledgements
9)
We would like to offer our cordial thanks to the
Research Division, University Grants Commission
(UGC) providing financial assistance for institutional
research in Kathmandu University. We are very
thankful to the Department of Biotechnology for giving
us laboratory facilities to carry out this project.
10)
11)
References
1)
2)
3)
4)
5)
12)
13)
14)
15)
16)
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