Red Part 1

at higher concentration

Dilution

Volume Stock Allura Final Volume soln

1 1.0 mL

10.0 mL

2
3

(note: we used the same cuvette fo

4
5
6
7
8
9
10

max

First Aliquot

absorption

502

0.814

503

0.815

502

0.815

502.3333333333

0.8146666667

0.5773502692

0.0005773503

At lower concentration

max

Dilution
1
2
3
4
5
6
7
8
9
10
Avg:

Absorption at max wavelength

499
0.901
499
0.919
499
0.917
499
0.919
499
0.914
499
0.918
500
0.907
500
0.95
500
0.957
500
0.953
499.4
0.9255
0.5163977795
0.0201011332

Part 4
Allura Red AC soln 1

Initial stock volume

Percentage of diluted stock

20

40

60

80

Blue 1 Soln

100

% diluted solution

Final [Blue 1]

10 1.12E-6 M

20 2.38E-6 M

40 4.76E-6 M

60 7.14E-6 M

80 9.52E-6 M

Initial concentration: 1.19E-4 M

Initial dilution: 1.19 E-5 M

Unknowns:
[Initial] Absorbance
Mountain Dew Code Red - dark retoo high, 1.347>1

[1/2 Initial] Absorbance

Observations: bubbly.
Aliquot 1

0.705

Aliquot 2

0.694

Aliquot 3

0.647

Strawberry Kiwi Gatorade Rain - ver

[Initial] Absorbance
Aliquot 1

8.44E-02

Aliquot 2

9.78E-02

Aliquot 3

9.43E-02

Mountain Blast Powerade

Aliquot 1

0.522

Aliquot 2

0.508

Aliquot 3

0.523

Gatorade Fierce Blue (grape)

Aliquot 1

1.209 (too high), >1

0.606

Aliquot 2

0.599

Aliquot 3

0.551

.258g/L
max

Absorption at max wavelength

80% dye content

molar absorptivity

502

0.815

0.00042 19404.76190476

502

1.025

0.00042 24404.76190476

505

1.234

0.00042 29380.95238095

502

1.289

0.00042 30690.47619048

502

1.308

0.00042 31142.85714286

501

0.871

0.00042

502

0.857

0.00042 20404.76190476

502

0.858

0.00042 20428.57142857

501

0.896

0.00042 21333.33333333

500

1.035

0.00042 24642.85714286

501.9

1.0188

0.00042 24257.14285714

1.2866839377

0.1925707951

0 4585.018930066

20738.0952381

19380.9523809524 Average max

19404.7619047619 avg
19404.7619047619
19396.8253968254
13.7464349807
80% dye content

[Allura Red AC]

molar absorptivity (Grubb's 90%

0.000039

23102.564102564

1.2188367575

0.000039

23564.102564103

0.323364854

0.000039

23512.820512821

0.4228617322

0.000039

23564.102564103

0.323364854

0.000039

23435.897435898

0.5721070494

0.000039

23538.461538462

0.3731132931

0.000039

23256.41025641

0.920346123

0.000039

24358.974358974

1.2188367575

0.000039

24538.461538462

1.567075831

0.000039

24435.897435898

1.3680820747

0.000039

23730.769230769

515.4136715598

Avg Absorbance

STDEV Abs

7.8E-6 M

0.1693333333

0.0220529665

1.56E-5 M

0.3643333333

0.0227229693

2.34E-5 M

0.54

0.0085440037

3.12E-5 M

0.725

0.0051961524

3.9E-5 M

[Blue 1]

max

0.9106666667

Avg Absorbance

0.0035118846

STDEV Abs
0.0990666667

0.0090522557

0.228

0.0566656863

0.422

0.0300499584

0.6196666667

0.0064291005

0.765

0.023430749

503
503
503

508
506
508

631
632
631

631
631
631

part 2
Grubb's 90%

Grubb's 95%

1.0583120869
0.0321959516
1.1175110947
1.4031203429
1.5017853559
0.7675099433
0.8402104792
0.8350175838
0.6376875578
0.0841249058

Grubb's 95%
1.2188367575
0.323364854
0.4228617322
0.323364854
0.5721070494
0.3731132931

Absorbance

Average

0.920346123 Table 2

1.308

1.019

1.2188367575 Table 4

0.957

0.923

1.567075831
1.3680820747

1st soln aliquot ( Absorbance

max

0.145

503

0.188

503

0.175

503

2nd soln aliquot ( Absorbance

max

0.385

501

0.34

501

0.368

3rd soln aliquot ( Absorbance

501
max

0.539

502

0.532

501

0.549

501

4th soln aliquot ( Absorbance

max

0.728

501

0.719

501

0.728

500

5th soln aliquot ( Absorbance

max

0.907

499

0.911

499

0.914

499

1st soln aliquot ( Absorbance

max

0.0993

625

0.108

625

0.0899

625

2nd soln aliquot ( Absorbance

max

0.174

633

0.223

629

0.287

628

3rd soln aliquot ( Absorbance

max

0.399

631

0.411

628

0.456

628

4th soln aliquot ( Absorbance

max

0.615

628

0.617

629

0.627

5th soln aliquot ( Absorbance

630
max

0.738

630

0.777

630

0.78

630

Average
Stdev

Standard deviation Grubbs test

0.193

1.4974093264

0.02

1.7

More dilute

Epsilon
21839 23102.56410256
27494

23564.1025641

27239 23512.82051282
22083

23564.1025641

27847

23435.8974359

21956 23538.46153846
22199 23256.41025641
21933 24358.97435897
27188 24538.46153846
22103

24435.8974359

24188.1 23730.76923077
2807.639160497 515.4136715598

0.6238385302

max

Absorption at max wavelength

502
0.815
502
1.025
505
1.234
502
1.289
502
1.308
501
0.871
502
0.857
502
0.858
501
0.896
500
1.035
501.9
1.0188

first 3
ave wavelength
stdev

503
1.73205081

first 5
ave wavelength
stdev

502.6
1.34164079

all 10
ave wavelength
stdev

501.9
1.28668394

Table 2
Table 4
Table 2
Table 4

Average
24300
23700
80%

T-value
ave absorption
stdev

1.02466667
0.2095002

ave absorption
stdev

1.1342
0.21091633

ave absorption
stdev

1.0188
0.1925708

Standard deviation
4590
515
90%

80% 1.38302874
90% 1.83311293
95% 2.26215716

80%
90%
95%
2007.40437 2660.57282 3283.26008
225.231645 298.51743 368.383211
95%

[Allura Red AC] Avg Absorbance Conc

abs
7.8E-6 M
0.1693333333 7.80E-06 0.16933333
1.56E-5 M
0.3643333333 1.56E-05 0.36433333
2.34E-5 M
0.54 2.34E-05
0.54
3.12E-5 M
0.725 3.12E-05
0.725
3.9E-5 M
0.9106666667 3.90E-05 0.91066667

param
stdev

slope
23632.4786
190.350918
0.99980541

Calibration curve of ab

0.

0.

0.

0.

0.
Absorbance 0.

0.

0.

0.

0.0

slope
int
0.0990666667 79493.4169 0.03055148
0.228 3392.08648 0.01984799
0.422 0.99456716 0.02325129
0.6196666667
0.765

1.12E-06
2.38E-06
4.76E-06
7.14E-06
9.52E-06

Calibration curve of abso

0.9

6.83E-07
1.45E-06
2.90E-06
4.36E-06
5.81E-06

0.8

0.0990666667
0.228
0.422
0.6196666667
0.765

0.7
0.6
0.5
Absorbance 0.4
0.3

0.9

0.2

0.8
0.7

0.1

f(x) = 130317.076922816x + 0.0305514767

0
0.00

0.6
0.5
0.4

Co
Column B
Linear (Column B)

0.3
0.9

0.2

0.8
0.7

0.1

f(x) = 130317.076922816x + 0.0305514767

0
0.00

0.6
0.5
0.4
0.3
0.2
0.1
0
0.00E+00 2.00E-06 4.00E-06 6.00E-06 8.00E-06

Co
Column B
Linear (Column B)

int
-0.01113333
0.00492432
0.00469515

23632.4786
190.350918
0.99980541

Calibration curve of absorbance versus concentration for Red 40

1
0.9
0.8
0.7

Error bars
0.02205297
0.02272297
0.008544
0.00519615
0.00351189

0.6
Column D

0.5
Absorbance 0.4

Linear (Column D)

0.3
0.2
0.1
0
2.00E-05
6.00E-05
0.00E+00
4.00E-05
Concentration in mol/L

Calibration curve of absorbance versus concentration for Blue 1

0.9
0.8
0.7
0.6
0.5
Absorbance 0.4
0.3
0.2
0.1
0
0.00E+005.00E-06 1.00E-05
Concentration in mol/L

Column B
Linear (Column B)

Error bars
0.00905226
0.05666569
0.03004996
0.0064291
0.02343075

0.3
0.2
0.1
0
0.00E+005.00E-06 1.00E-05
Concentration in mol/L

-0.01113333
0.00492432
0.00469515
Unknowns:
[Initial] Absorbance
Mountain Dewtoo high, 1.347>1
Observations: bubbly.
Aliquot 1
Aliquot 2
Aliquot 3
Strawberry Kiw
[Initial] Absorbance
Aliquot 1
Aliquot 2
Aliquot 3
Mountain Blast Powerade
Aliquot 1
Aliquot 2
Aliquot 3

[1/2 Initial] Absorbancemax

0.705
0.694
0.647

503
503
503

8.44E-02
9.78E-02
9.43E-02

508
506
508

0.522
0.508
0.523

631
632
631

Gatorade Fierce Blue (grape)

Aliquot 1
1.209 (too high), >1
Aliquot 2
Aliquot 3

Mountain Dew Code Red

Strawberry Kiwi Gatorade Rain
Mountain Blast Powerade
Gatorade Fierce Blue

0.606
0.599
0.551

631
631
631

3.03432E-005
2.98771E-005
2.78856E-005
average
stdev
4.05E-06
4.62E-06
4.47E-06
average
stdev
6.18113E-006
0.000006005
6.19371E-006
average
stdev
7.23774E-006
7.14969E-006
6.54591E-006
average
stdev

correcting for dilution correct for blue

6.0686440678E-005
5.9754237288E-005
5.5771186441E-005
5.8737288136E-005
2.6106647120E-006

4.38E-06
0.000000295

6.1266247379E-006
1.0549052935E-007
1.4475471698E-005
1.4299371069E-005
1.3091823899E-005
1.3955555556E-005
7.5317802563E-007

0.00000378
3.67231E-006
3.78769E-006
3.74667E-006
6.45115E-008
8.85231E-006
8.74462E-006
8.00615E-006
8.53436E-006
4.60597E-007

Compare Means
Descriptive Statistics
VAR

Sample size Mean

21839

24,449.11

23102.5641

23,800.57

Standard Deviation Variance

2,846.36

494.01044 244,046.32

t-test assuming unequal variances (heteroscedastic)

Degrees of Freedom

Hypothesized Mean Difference

Pooled Variance
Test Statistics

###
0.67348

Two-tailed distribution
p-level

0.51962 Critical Value

2.306

0.25981 Critical Value

1.85955

One-tailed distribution
p-level

###