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During the last two decades there has been in search for new plant
derived drugs containing the medically useful alkaloids, glycosides,
polyphenolics, steroids and terpenoids derivatives, which contributes
to the antioxidant property11. Dietary phenolic compounds and
flavonoids have generally been considered, as non-nutrients and their
possible beneficial effect on human health have only recently been
recognized. Flavonoids are known to possess antioxidant and
anticarcinogenic activities12. Therefore search for natural antioxidants
of plant origin gained momentum in recent years.
The main objectives of the study are to analysis the total phenolic
and flavonoid content, reducing power, antioxidant activity.
METHODS AND MATERIALS
Chemicals used
The seeds were shade dried for one month and coarsely powdered.
Extraction of the active ingredient from the seed powder was
carried out using specific method15. 25g of the powdered seeds were
extracted by soxhlet apparatus using 250ml of four different solvent
(ethanol, acetone, ethylacetate and water) in a separate flask. The
extraction lasted for six hours. The extract obtained was
concentrated by evaporation using water bath at 100C and stored
at 4C in cold room.
Shanmugapriya et al.
Ac Ae
Ac
x 100
Where,
257
Shanmugapriya et al.
to
their
electron
The DPPH radical has been widely used to test the ability of
compounds as free-radical scavengers or hydrogen donors and to
evaluate the antioxidative activity of plant extracts and foods42, 43.
The DPPH scavenging activity was found to be dose dependent. This
radical scavenging activity of plants extracts could be related to the
Table 1: Total phenolic and flavonoid content, Reducing potential of different seeds extracts of Artocarpus heterophyllus and Manilkara
zapota
Seed extracts
Artocarpus
heterophyllus
Manilkara
zapota
Different fractions
Ethanolic
Acetone
Ethyl acetate
Aqueous
Ethanolic
Acetone
Ethyl acetate
Aqueous
All values are expressed as Mean SD for three determinations; GAE- Gallic acid equivalent; QE -Quercetin equivalent
Reducing potential
(g/ml)
13.120.01
10.520.02
10.130.01
09.560.02
13.960.01
10.430.01
10.480.02
09.410.02
[[[[
Table 2: DPPH radical scavenging assay of different seeds extracts of Artocarpus heterophyllus and Manilkara zapota
Seed extracts
Artocarpus
heterophyllus
Manilkara zapota
Different
fractions
Ethanolic
Acetone
Ethyl acetate
Aqueous
Ethanolic
Acetone
Ethyl acetate
Aqueous
500 g/ml
67.770.01
56.780.02
52.280.01
51.800.02
64.490.01
52.830.01
51.990.02
50.460.01
IC 50
Values
(g/ml)
300
390
490
480
290
400
490
500
Table 3: ABTS radical scavenging assay of different seeds extracts of Artocarpus heterophyllus and Manilkara zapota
Seed extracts
Artocarpus
heterophyllus
Manilkara zapota
Different fractions
Ethanolic
Acetone
Ethyl acetate
Aqueous
Ethanolic
Acetone
Ethyl acetate
Aqueous
500 g/ml
62.010.01
52.780.01
50.360.01
52.040.02
60.660.01
56.050.01
50.670.01
51.750.01
IC 50
Values
(g/ml)
290
380
500
480
300
390
500
490
258
Shanmugapriya et al.
CONCLUSION
The results obtained in the present study, it was concluded that the
seed extracts of both plants contains large amounts of flavonoids
and phenolic compounds, exhibits high antioxidant and free radical
scavenging activities in addition to having their medicinal
properties. It also chelates iron and has reducing power. Both plant
extract is a significant source of natural antioxidant, which might be
helpful in preventing the progress of various oxidative stresses.
Therefore, studies on these medicinal plants would be of great
importance. Both plants are widely used in number of
pharmacological actions with high content of flavonoids and phenol
seems to have a high potential for antioxidant activity.
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