RESULTS

1. How many units of EBA chromatography needed for this production? Is it

economically feasible?

The above figure shows that there are 5 multiple units of the EBA chromatography.

The above figure shows that there is no revenue for the process and the gross margin is
-1%. Besides that, the return on investment is also very low, which is -57.33%. Therefore
the process is not feasible. Further analysis and modifications is needed to optimize the
process.

2. What is the amount of product obtained? How about the purity of the product?

The amount of the product, MAB crystal obtained is 7.24 kg/batch and 90% by mass
composition.
3. If you are a bioprocess engineer appointed to set up this plant, how would you set
up the process design so that the production is economically feasible and
produce more products than the current process design without adding any
additional cost. Produce your recommended process design on a new process
flow sheet and produce the materials and stream reports to justify your design is
a best process design.
To modify the process of production of MAB so that it is economically feasible with more
products ant at no additional cost, there are a few recommendations that can be done.
Generally, the proposed solution include to reduce the number of chromatography
column and to increase the number of cycles in it. After these options have been
simulated, these are the results that were generated:

The figure above shows

the process after some modifications have been done; adding the number of cycles
in the chromatography. By doing so, the number of the units is reduced to 2.
Consequently, the equipment cost is reduced and the production of the products is
increased.

From the Economic Evaluation Report, it can be observed that the value of the
Return on Investment, while still negative, has been reduced, compared to the previous
process. Therefore, if not very feasible, at least it is more feasible than the previous process.
Calculation settings in the simulations need to be adjusted as not much skills and knowledge
is known to handle that. In addition to that, the duration for the complete recipe has been
increased to approximately 65 hours, compared to the 60 hours needed in the previous
process.

The amount of the product, MAB crystal obtained is 8.02 kg/batch, which is slightly
more than the products produced by the previous batch, at 7.24 kg/batch. However, the
purity is not increased. More high-end purification techniques probably need to be used in
the process. However, due to the lack of knowledge and skills, the option was not able to be
simulated.

CONCLUSION
This process needs a total of 59.90 hours to complete, and produces 7.24 kg/batch of MAB
with 90% purity. This is considered small due to the long duration of the process. Therefore
calculations need to be revised so that the production can be maximized optimally. The unit
operation data need to be considered very tentatively as it is important in determining the
purity of the products. It is also not very feasible as its Economic Evaluation Report stated
that its Return on Investment is more than -50%.
A recommendation to the betterment of the process included the increment of
number of cycles in the chromatography. This has resulted in prolonged process duration,
but more product yield and reduced equipment cost. In order to highly optimize the process,
more knowledge and skills is needed to adjust the data and information needed during the
simulation of the process.