Professional Documents
Culture Documents
, 2(5): 552-558
(ISSN: 2455-1716)
SEPTEMBER-2016
Address for Correspondence: Susanta Roy Karmakar, Assistant Professor, Department of Zoology, Maulana Azad
College, Kolkata, West Bengal, India
Received: 09 June 2016/Revised: 17 July 2016/Accepted: 10 August 2016
ABSTRACT- Objective: In the present study, acute toxicity of neem (Azadirachta indica A. Juss) leaf extracts on the
freshwater unwanted fish, Channa gachua (Ham) was investigated.
Materials and Methods: During the study period, ethological responses in the exposed fish (240 in number irrespective
of sex) were observed. Changes in some haematological parameters like haemoglobin content (Hb), haematocrit (Ht),
total erythrocyte count (TEC), mean corpuscular volume (MCV), mean corpuscular haemoglobin (MCH), mean
corpuscular haemoglobin concentration (MCHC) and blood glucose (Glu) were also measured in the exposed fish (60 in
number irrespective of sex) during the acute toxicity test at different time interval.
Results: The 24, 48, 72 and 96 h LC50 values for C. gachua were 21.80, 19.59, 13.95 and 11.18 g/l respectively. Toxicity
factor values of this phytopiscicide to the fish were increased with the progress of exposure time. They showed an
alteration in their responses with the increasing concentration of neem leaf extracts and time of exposure. A significant
elevation in the level of blood glucose in the exposed fish throughout the exposure period was recorded while Hb showed
significant change after 24 h and that of MCHC only at 96 h.
Conclusions: The study may help to determine the toxic level of the aqueous extracts of neem leaves to the unwanted fish
in aquaculture farm and to understand the mode of its action to fish behaviour and haematology.
Key-words: Acute toxicity, Azadirachta indica, Snake Headed Fish, Haematological Parameters, Ethological Responses
-------------------------------------------------IJLSSR-----------------------------------------------
INTRODUCTION
Website:
www.ijlssr.com
DOI:
10.21276/ijlssr.2016.2.5.7
http://ijlssr.com
Page 552
RESULTS
Page 553
Table 1. Acute lethal concentrations (LC) with 95% confidence limits of neem leaf extracts to Channa gachua
(Control group theoretical spontaneous response rate= 0.0000)
Lethal concentrations
(g/l)
LC1
24h
48h
72h
96h
9.23
(2.44-11.93)
6.43
(1.94-8.96)
4.50
(1.79-6.44)
3.78
(1.61-5.44)
21.80
(18.34-36.57)
19.59
(16.44-31.56)
13.95
(11.94-16.79)
11.18
(9.33-12.96)
51.46
(31.46-79.93)
49.73
(35.01-72.40)
43.24
(29.21-59.22)
13.58
(8.43-15.74)
LC10
LC50
10.60
(6.28-12.73)
36.21
(25.31-63.12)
LC90
LC99
7.48
(4.47-9.30)
34.99
(24.92-52.74)
6.15
(3.66-7.78)
26.01
(20.32-47.56)
20.32
(16.70-30.71)
33.07
(24.03-68.29)
Table 2. Toxicity factor of neem leaf extracts to Channa gachua at LC50 value under different exposure periods
Exposure time (h)
48
1.11
24
1.00
72
1.56
96
1.95
Table 3. Impact of neem leaf extracts on ethological responses of Channa gachua at different time of exposure and
concentrations (none: -; mild: +; moderate: ++; strong: +++)
Ethological
parameters
Fin
movement
Exposure
time (hr)
24
48
72
Opercula
movement
96
24
48
72
Jumping
movement
96
24
48
72
Swimming
rate
96
24
48
72
96
http://ijlssr.com
0(control)
12
14
16
18
20
+++
+++
+++
++
++
++
++
++
+++
++
++
++
+++
+++
+++
++
++
++
++
++
++
++
++
+++
+++
+++
++
+++
+++
+++
+++
+++
+++
++
++
++
++
+
+++
+++
+++
+++
++
++
++
+++
++
+++
++
++
+++
+++
+++
+++
+++
+++
+++
+++
++
++
+++
+++
+++
++
++
++
++
+
++
++
++
+++
++
++
++
+
++
+
++
++
++
++
++
+++
++
+++
+++
++
++
++
+++
+++
++
++
+++
+++
++
+++
+++
+++
++
++
++
++
++
++
+++
++
++
Page 554
24
+++
+++
+++
+++
++
++
++
++
++
48
+++
+++
+++
++
++
++
++
++
++
96
+++
+++
24
+++
+++
+++
+++
48
+++
+++
++
++
96
+++
++
72
Equilibrium
status
72
Staying
period
24
48
72
Mucous
secretion
96
24
48
72
96
+++
+++
++
+++
++
++
-
++
++
-
+++
++
++
++
++
++
++
+
++
+
++
++
++
+++
+
+
+
-
+
+
-
++
++
++
+++
+++
+++
+
++
++
+++
+++
-
Fig.1. Histogram showing mean values ( SE) of some haematological parameters in Channa gachua exposed to
neem leaf extracts at different times of exposure (24, 48, 72 and 96h)
Changes in haematological parameters in exposed fish at different times during acute toxicity study were summarized in
Fig.1. The present study demonstrated that the fish exposed to neem leaf extracts displayed a significant elevation in the
level of blood glucose throughout the exposure period over the control. But haemoglobin content showed significant
changes at 48, 72 and 96 h, whereas mean corpuscular haemoglobin concentration showed significant changes only at 96
h.
http://ijlssr.com
Page 555
DISCUSSION
CONCLUSION
ACKNOWLEDGMENT
REFERENCES
Page 556
236-244.
[4] Mousa MAA, El-Ashram AMM and Hamed M. (Effect of
neem leaf extract on freshwater fishes and zooplankton
community). Proceedings of the 8th International
Symposium on Tilapia in Aquaculture, Central Laboratory
for Aquaculture Research, October 12-14, Cairo, Egypt,
2008: 307318.
[5] Saravanan M, Ramesh M, Malarvizhi A and Petkam R.
(Toxicity of neem leaf extracts (Azadirachta india A. Juss)
on some haematological, ionoregulatory, biochemical and
enzymological parameters of Indian Major Carp, Cirrhinus
mrigala). Journal of Tropical Forestry and Environment,
2011; 1(1): 14-26
[6] Fafioye OO. (Acute and sub-acute toxicities of five plant
extracts on white tilapia, Oreochromis niloticus (Trewavas)).
International Research Journal of Agricultural Science and
Soil Science, 2012; 2(13): 525-530.
[7] Dhara K, Biswas SJ and Roy Karmakar S. (Study on the
toxicity of neem (Azadirachta indica A. Juss) leaf extracts as
phytopiscicide on three life stages of Mozambique tilapia
(Oreochromis mossambicus Peters) with special reference to
their ethological responses). Int J Exp Res Rev, 2016; 3:
7-13.
[8] Gradi P, Domarco RC and Pinheiro CWL. (Avaliao do
uso de inseticida (orgnicos fosforados) no combate as
Odonatas e na seleo zooplanctnica em piscicultura de
alevinagem). Estudos de Piscicultura, 1988; Braslia, Brazil.
[9] Martinez SO. (NIM-Azadirachta indica: natureza, usos
mltiplose produo). Instituto Agronmico do Paran
(IAPAR), 2002; Londrina, PR.
[10] Saravanan M, Vasantha Kumar D, Malarvizhi A and Ramesh
M. (Biosafety of Azadirachta indica (A. Juss) leaves extracts
on certain biochemical parameters of Labeo rohita). Journal
of Biopesticides, 2010; 3(1 Special Issue): 227 231.
[11] Alam A, Tabinda AB, Mahmood-ul-Hassan and Yasar A.
(Comparative toxicity of acetamiprid and Azadirachta indica
leave extract on biochemical components of blood of Labeo
rohita). Pakistan J Zool, 2014; 46 (6): 1515-1520.
[12] Cruz C, Machado-Neto JG, Menezes ML. (Toxicidade aguda
do inseticida Pration metilico e do biopesticida azadiractina
de folhas de neem (Azadirachta indica) para alevino
e juvenil de pacu (Piaractus mesopotamicus). Pesticidas: R
Ecotoxicol e Meio Ambiente, 2004; 14: 92-102.
[13] APHA. Standard methods for the examination of water and
wastewater. Rice EW, Baird RB, Eaton AD and Clescen, LS
(Eds.) American Public Health Association, American Water
Works Association (AWWA) and Water Environment
Federation (WEF), 2012; Washington DC.
[14] US EPA. Probit program version 1.5. Ecological Monitoring
Research Division, Environmental Monitoring Systems
Laboratory, US Environmental Protection Agency, 1999;
Cincinnati, Ohio 45268.
http://www.epa.gov/nerleerd/stat2.htm.
[15] Finney PJ. Probit analysis, 3rd ed., Cambridge University
Press, 1971; pp. 333-337.
[16] Ayoola SO, Kuton MP, Idowu AA and Adelekun AB. (Acute
toxicity of Nile tilapia (Oreochromis niloticus) juveniles
exposed to aqueous and ethanolic extracts of Ipomoea
aquatica leaf). Nature and Science, 2011; 9(3): 91-99.
[17] Rand GM. Behavior. In: Fundamentals of Aquatic
Toxicology Methods and Applications, Rand GM and
Petrocelli SR (Eds), Hemisphere Publishing Corporation,
Washington, 1985; pp. 221-262.
http://ijlssr.com
Page 557
http://ijlssr.com
Page 558