Other Fluorescent Labels:

Fluorescein
Isothocyanate
(FITC)- most commonly used
Phycocyanin
Texas Red
Tetramethyl rhodamine

*QUENCHINGdisadvantage
fluorescent assay;

o Serum Preservation
PHYSICAL: refrigerate at
4-6OC for 72 hours
CHEMICAL: add 0.001g
merthionate powder per
mL of serum; or 5% phenol
or Tricresol at 0.1 mL/mL
of serum

of

any process that decrease

fluorescence
TORCH- Other infections: Cocksackie
virus
For CHEMILUMINISCENCE, measure it
using LUMINOMETER if LUMINOL was
used in the assay

Chicken pox
Chlamydia
HIV, HTLV

(+) EIA: color change (depends on

chromogen used)

Syphilis
Zikafever

OTHER IMMUNOLOGIC TESTS

RAJI CELL ASSAY- measures

immune complexes
TPI- most specific test for
syphilis; standard test for which
other treponemal antibody tests
are evaluated
o Serum Inactivation- heat at
560C for 30 mins or heat at
60-62OC for 3-4 mins
chemical: use choline
chloride
(ex. RPR)

*Ford-Jones & Kelner (1995)

C- Chicken pox and shingles

H- Hepatitis
E- Enteroviruses
H- HIV/ AIDS
P- Parvovirus B19

T- Toxoplasmosis
O- Other Infections

3. MONOSPOT TEST

R- Rubella

PRINCIPLE:
Hemagglutination

Absorption

C- CMV
Reagent: Horse RBCs
H- Herpes Simplex
E- Everything else sexually transmitted
(gonorrhoea, Chlamydia, HIV)

Most sensitive
Antibodies in

indicator

of

IM
S- Syphilis
Causes of production of heterophile
antibodies
IM- EBV

TEST FOR IM

SERUM SICKNESS- horse serum used

in immunization
1. PAUL-BUNNELL Test- screening
test
to
detect
heterophile
antibodies
PRINCIPLE: Hemagglutination
Reagent: Sheep RBCs
(+) agglutination
2. DAVIDSON
DIFFERENTIAL
TEST-used
to
deifferentiate
heterophile antibodies
PRINCIPLE:
Absorption
Hemagglutination
Reagent: Guinea pig kidney cells
and Beef Erythrocyte Antigens
(BEA)
Indicator: Sheep RBCs
GPK BEA
+

FORSMANN- Salmonella, Shigella and

other bacterial infection

Additional Info:
HIV- infective in dried blood/ smears for
3 days at room temp; killed by bleach
within 2 minutes
HBV-infective outside for 7 days; killed
by bleach within 10 minutes

Forssman

Serum sickness

Volume of blood to be drawn: 450 mL+

30 mL

IM

BP: 90-160 - systolic

BB

60-100 - diastolic
AABB: systolic: not more than 180
diastolic: not more than 100

4. Blood collection
- At least 2 personnel must be
present (phlebotomist)
5. Bleeding: 7-10 mins.
Philippines: not more than 15
mins.

Donation Process
1. Donor registration
2. Physical exam
3. Donor Selection

DISEASES
Trichinosis

SEROLOGIC
TEST
Bachman
Intradermal Test

Tuberculosis

Tuberculin Skin
Test
Von Priquets Test
Vollmers Patch
Mendels Test
Mantoux Test

Brucellosis

Brucellergin Skin
Test
Coccidioidodin Skin
Test
Frei Test

Coccidioidomycosis
Lymphogranuloma
venereum
Histoplasmosis
Diphtheria
Scarlet fever
Glanders disease
Toxoplasmosis
S. pneumoniae
Anthrax
Ascariasis
Hydatid

Histoplasmin
Schicks Test
Dicks Test
Mullein Test
Toxoplasmin Skin
Test
Francis Skin Test
Ascoli Test
Moan Test
Casoni Intadermal
Test