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Answers
1. d) Altmann
2. a) Sequence of nucleic acids
3. c) Studies of Bacterial transformation
4. a) Feulgen and Rossenbeck
5. d) Chargaff
6. a) A+G / T+C
7. b) Basic Fuchsin
8. d) both b and c
9. c) 3
10. c) X-Ray crystallography
MCQ on Biochemistry - Nucleic acids (RNA)
1. RNA is the genetic material in
a) Viruses only
b) In some viruses and some prokaryotes
c) In some viruses and some prokaryotes and rarely in eukaryotes
d) Only in some viruses
2. RNA is
a) Single stranded
b) Double stranded
c) Triple stranded
d) Both a and b
3. The sugar in RNA is
a) Deoxyribose
b) Ribose
c) Hexose
d) Fructose
4. Nucleotides in RNA are joined by
a) 35 phosphodiester bond
b) 34 phosphodiester bond
c) 32 phosphodiester bond
d) 36 phosphodiester bond
5. Thymine in DNA is replaced by
a) Guanine in RNA
b) Adenine in RNA
c) Cytosine in RNA
d) Uracil in RNA
6. The most abundant type of RNA in the cell is
a) rRNA
b) mRNA
c) tRNA
d) hnRNA
7. Which of the following RNA serves as adaptor molecule during protein synthesis
a) rRNA
b) mRNA
c) tRNA
d) hnRNA
8. rRNA is synthesised in
a) nucleus
b) Cytoplasm
c) RER
d) Nucleolus
9. cDNA is
a) complementary to mRNA
b) complementary to rRNA
c) complementary to tRNA
d) complementary to hnRNA
10. Amino acids are attached to the
a) acceptor arm of tRNA
b) anti-codon arm of tRNA
c) codon arm of tRNA
d) none of these
Answers
2-a
7-c
12-b
3-b
8-d
13-a
4-a
9-a
14-d
5-d
10-a
15-b
d) Chloroplast
7. Microfilaments are composed mainly of a protein called
a) actin
b)tubulin
c) myosin
d) chitin
8. Experiments demonstrating the importance of the nucleus in controlling the
growth of the cell was performed in
a) Starfish
b) Acetabularia
c) Neurospora
d) Leucocytes
9. Which of the following is associated with the structure of Golgicomplex?
a) Cristae
b) Cisternae
c) Annuli
d) Quatasomes
10. The subunit of prokaryotic ribosomes are
a) 60 S+40 S
b) 70 S+30 S
c) 60 S+30 S
d) 50 S+30 S
11. Plant cell wall mainly composed of
a) cellulose
b) starch
c) protein
d) lipid
12. Smooth endoplasmic reticulam is the site of
a) protein synthesis
b) carbohydrate synthesis
c) amino acid synthesis
d) Lipid synthesis
13. In higher plants, the shape of the chloroplast is
a) Discoid
b) cup shaped
c) girdle shaped
d) ribbon shaped
14. The main function of Centrosome is
a) Secretion
b) osmoregulation
c) Protein synthesis
d) Formation of spindle fibre
15. Assembly of two subunits 40 S and 60 S of the ribosome is
a) 100 S unit
b) 80 S unit
c) 70 S unit
d) 90 S unit
Answers:
1- b
6-a
11-a
2- c
7-a
12-d
3-c
8-b
13-a
4-b
9-b
14-d
5-b
10-d
15-b
c) Altman
d) Benda
3. Myocardial muscle cells have numerous large mitochondria called as
a) myocardial mitochondrions
b) mitosomes
c) myosomes
d) sarcosomes
4. The number of mitochondria in a cell depends on
a) pH of the cell
b) shape of the cell
c) both a and b
d) functional state of the cell
5. Which of the following statements are true about mitochondria
HYPERLINK "http://www.biologyexams4u.com/2013/02/mitochondriastructure-and-function.html"
a) Mitochondria has two envelopes
b) Outer membrane has porin protein channels
b) Inner membrane is not permeable
c) all of these
6. Cristae are
a) The membraneous infoldings of the outer membrane of mitochondria
b) the jelly like central matrix of mitochondria
c) The membraneous infoldings of the inner membrane of mitochondria
d) enzymes located on the inner mitochondrial membrane
7. F0-F1 particles meant for ATP synthesis are present on
a) outer mitochondrial membrane attached to the cytosolic or C face
b) outer mitochondrial membrane attached to the Matrix or M face
c) inner mitochondrial membrane attached to the Matrix or M face
d) inner mitochondrial membrane attached to the cytosolic or C face
8. Which of the following stain is used to visualise mitochondrion
a) Janus green
b) Acetocarmine
c) Haematoxylin
d) Orange G
9. Which of the following is the histochemical marker for mitochondrial inner membrane
a) monoamine oxidase
b) cytochrome oxidase
c) malate dehydogenase
d) adenylate kinase
10. Mitoplast are
a) mitochondria without membranes
b) mitochondria without outer membrane
c) mitochondria without inner membrane
d) mitochondrial plastids
Answers
1. c) both a and b
2. d) Benda
3. d) sarcosomes
4. d) functional state of the cell
5. c) all of these
6. c) The membraneous infoldings of the inner membrane of mitochondria
7. c) inner mitochondrial membrane attached to the Matrix or M face
8. a) Janus green
9. b) cytochrome oxidase
10. b) mitochondria without outer membrane
a) Plasmosome
b) Plasmodesmata
c) Mesosome
d) All of these
12. Which of the following organelle is involved in cell wall synthesis
a) Mitochondria
b) Chloroplast
c) Golgi apparatus
d) lysosome
13. Which of the following statements are true regarding cellulose synthesis
a) Cellulose is synthesized on the external surface of the cell
b) The enzyme involved is a plasma membrane bound complex called
cellulose synthetase
c) UDP glucose is the precursor of cellulose
d) All of these
14. Secondary cell wall of plants is
a) Located outside the primary wall
b) Located inside the plasma membrane
c) Located inside the primary wall
d) Located just beneath middle lamellae
15. Which of the statements are incorrect regarding plant cell wall
a) Primary and secondary walls are present in meristamatic cells
b) Secondary cell wall consists of three concentric layers (S1, S2 and S3)
one after the other
c) In certain plants, tertiary cell wall is also present which has xylan beside
cellulose
d) Middle lamella is made up of pectin and lignin
Answers:
1. b) Bacteria, fungi and plants
2. d) N-acetyl glucosamine, N-acetyl muramic acid and amino acids
3. d) Cell was is semi-permeable
4. a) Cellulose, hemicelluloses and pectin
5. d) Fungi
6. c) Ruthenium red
7. c) 1-4 linkage
8. a) Cellulose
9. c) Glucoronic and galacturonic acid
10. c) Lignin
11. b) Plasmodesmata
12. c) Golgi apparatus
13. d) All of these
14. c) Located inside the primary wall
15. a) Primary and secondary walls are present in meristamatic cells
5. SER is involved in
a) Phospholipid biosynthesis and detoxification reaction
b) Phospholipid biosynthesis and protein synthesis
c) Protein synthesis
d) Phospholipid biosynthesis
6. SER in the retinal cells are called as
a) Sarcoplasmic reticulum
b) Retinal reticulam
c) Myeloid bodies
d) Amyloid bodies
7. The functions of RER include
a) Protein synthesis and detoxification
b) Protein synthesis and post translational modification
c) Protein synthesis and phospholipid biosynthesis
d) Protein synthesis only
8. Which of the following statements are true regarding endoplasmic reticulum
a) ER provides structural framework to the cell
b) ER acts as an intracellular transporting system
c) SER is involved in synthesis of lipids
d) All of these
9. The transport of secretory proteins takes place trough organelles in the order
a) RER->SER->golgi-> secretory vescicles
b) SER->RER->golgi-> secretory vescicles
c) RER->SER-> secretory vescicles -> golgi
d) RER->golgi->SER-> secretory vescicles
10. Rough Endoplasmic Reticulum is called rough because
a) rough texture of the surface
b) surface is studded with membrane proteins
c) surface is studded with ribosomes
d) All of these
11. RER is involved in the synthesis of
a) Membrane proteins and secretory proteins
b) Different proteins of the cell
c) Membrane proteins, secretory proteins and lysosomal proteins
d) Membrane proteins and secretory proteins and nuclear proteins
12. Which of the following organelle is involved in xenobiotic detoxification
a) Golgi
b) Lysosome
c) SER
d) RER
13. Protein glycosylation occurs in the
a) lumen of mitochondria
b) lumen of rough endoplasmic reticulum
c) lumen of smooth endoplasmic reticulum
d) lumen of lysosomes
14. Which of the following sequence functions as signals for N-linked glycosylation
a) Asn-X-Ser and Asn-X-Thr
b) Asn-X-Ser and Asn-X-Pro
c) Asn-X-Thr and Asn-X-Gly
d) Asn-X-Gly
15. Ribophorins are
a) transmembrane glycoprotein on RER
b) transmembrane glycoprotein on SER
c) luminal proteins on RER
d) luminal proteins on SER
Answers
1. a) The adipose tissue has both SER and RER
2. b) Porter
3. c) RER
4. a) the cytoplasmic side
5. a) Phospholipid biosynthesis and detoxification reaction
6. c) Myeloid bodies
7. b) Protein synthesis and post translational modification
8. d) All of these
9. a) RER->SER->golgi-> secretory vescicles
10. c) surface is studded with ribosomes
11. a) Membrane proteins and secretory proteins
12. c) SER
13. b) lumen of rough endoplasmic reticulum
14. a) Asn-X-Ser and Asn-X-Thr
15. a) transmembrane glycoprotein on RER
a) zone of inhibition
b) zone of exclusion
c) both a and b
d) organelle inhibition zone
6. GERL region is the
a) Golgi+RER+SER+lysosome+vescicles association involved in packaging
and sorting of cellular materials
b) Golgi+RER+SER+lumen of cytoplasm association involved in packaging
and sorting of cellular materials
c) Golgi+ER+lysosome association involved in packaging and sorting of
cellular materials
d) none of these
7. Histochemical localisation of Golgi is by staining with
a) Bismarck red
b) Fast green
c) Osmium tetroxide
d) acetocarmine
8. Which of the following organelle is involved in cell plate formation
a) SER
b) RER
c) lysosme
d) Golgi apparatus
9. The functions of Golgi apparatus include all except
a) GA is the sorting centre of the cell
b) GA is involved in post translational modification
c) GA is involved in secretory protein synthesis
d) GA is involved in cell plate formation
10. Which of the following statements are true regarding golgi apparatus
a) GA has polarity
b) cis-face is located close to either nucleus or transitional ER
c) trans-face is located near plasma membrane
d) all of these
Answers
1. c) Golgi apparatus
2. b) Silver stain
3. b) golgi apparatus of plant cells and lower invertebrates
4. d) cisternae
5. b) zone of exclusion
6. c) Golgi + ER + lysosome association involved in packaging and sorting of cellular
materials
7. c) Osmium tetroxide
8. d) Golgi apparatus
9. c) GA is involved in secretory protein synthesis
10. d) all of these
11. Which one of the following organelles is located near the nucleus and contains a
collection of flattened membrane bound cisternae?
a) Nucleus
b) Centriole
c) Mitochondrion
d) Golgi apparatus
12. Golgi complex was first recognised in
a) blood cell
b) root cell
c) nerve cell
d) root cell
13. Besides giving out secretary vesicles, the Golgi apparatus is also concerned with
the formation of
a) Nucleus
b) plastids
c) nucleus
d) Lysosomes
14. Zone of exclusion is associated with
a) Nucleus
b) Nucleolus
c) Nucleoplasm
d) Golgi complex
15. Golgi apparatus is absent in
a) higher plants
b) yeast
c) bacteria and blue green algae
d) none
16. Acid phosphatase is the cytochemical marker for
a) cis-region of GA
b) trans region of GA
c) GERL region
d) median region of GA
17. After synthesis of secretory protein in RER, it moves through
a) RER->cis Golgi->median golgi->trans golgi-> secretory vesicle
b) RER->trans Golgi->median golgi->cis golgi-> secretory vescicel
c) RER-> secretory vescicels ->cis Golgi->median golgi->trans golgi->
secretory vescicel
d) all of these
18. Golgi apparatus is often seen associated with
a) mitochondria
b) RER
c) lysosome
d) none of these
19. Which of the following organelle is called as the sorting centre of the cell
a) RER
b) SER
c) GA
d) lysosome
20. Function ofGolgi apparatus (GA) in animal cells include
a) sorting and packaging
b) exocytosis of melanin granules
c) exocytosis of thyroxine hormone
d) all of these
Answers
11. d) Golgi apparatus
12. c) nerve cell
13. d) Lysosomes
14. d) Golgi complex
15. c) bacteria and blue green algae
16. c) GERL region
17. a) RER->cis Golgi->median golgi->trans golgi-> secretory vesicle
18. b) RER
19. c) GA
20. d) all of these
c) Chara
d) Scendesmus
4. Nucleus are absent in
a) Red blood cells and bacterium
b) Red blood cells, sieve cells and bacterium
c) Red blood cells only
d) none of these
5. Which of the following statements is true regarding the size of nucleus?
a) The size of the nucleus is directly proportional to that of the cytoplasm
b) The size of the nucleus depends on the total cell volume
c) The size of the nucleus depends on the chromosome number or ploidy level of the
cell
d) all of these
6. Nucleus has
a) DNA only
b) DNA and protein only
c) DNA, RNA and proteins
d) none of these
7. Nuclear membrane is in continuous connection with
a) SER
b) RER
c) Golgi apparatus
d) lysosomes
8. The protein network that lines the inner side of nuclear membrane is called
a) Nucleolus
b) nuclear matrix
c) nuclear lamina
d) nuclear proteins
9. The number of nuclear pores depends on the
a) size of the cell
b) transcriptional activity of the cell
c) DNA content of the cell
d) all of these
10. The most important function of nuclear envelope is to
a) regulate nucleo cytoplasmic traffic
b) protect genetic material
c) prevent the entrance of active ribosomes into the nucleus
d) synthesis rRNAs
11. The DNA protein ratio in chromatin is
a) 3:1
b) 2:1
c) 1:1
d) 4:1
12. Nucleolus is a prominent acidophilic spherical bodies in the nucleus. The
function is
a) RNA synthesis
b) DNA synthesis
c) histone synthesis
d) ribosomal subunit synthesis
13. The basic proteins of the nucleus are
a) nucleohistones
b) nucleoprotamines
c) both a and b
d) none of these
14. The major amino acids in histones are
a) Gluatamate and aspartic acid
b) lysine and arginine
c) arginine, lysine and histidine
d) histidine
15. The light stained and diffused region of chromatin is known as
a) Heterochromatin
b) Euchromatin
c) chromatin
d) none of these
Answers
1. c) Robert Brown
2. a) nucleus
3. b) Acetabularia
4. b) Red blood cells, sieve cells and bacterium
5. d) all of these
6. c) DNA, RNA and proteins
7. b) RER
8. c) nuclear lamina
9. b) transcriptional activity of the cell
10. c) prevent the entrance of active ribosomes into the nucleus
11. c) 1:1
12. d) ribosomal subunit synthesis
13. c) both a and b
14. c) arginine, lysine and histidine
15. b) Euchromatin
b) Spindle formation
c) Formation of cell plate
d) Chromosome doubling
10. If you are provided with root tips of onion in your class and are asked to count the
chromosomes, which of the following stages can you most conveniently look into?
a) Prophase
b) Metaphase
c) Anaphase
d) Telophase
Answers
1. b) Early prophase
2. b) Meiosis II
3. c) Metaphase
4. b) line up at the equator
5. a) two each in mitosis and meiosis
6. b) Telophase
7. d) Same number of chromosome and half number of chromatids
8. a) 7
Explanation: Each cell produces 2 daughter cells, therefore after 7 divisions one cell will
give 128 cells.
1 (I) >2 (II) > 4 (III) > 8 (IV) > 16 (V) > 32(VI) > 64 (VII) > 128
9. b) Spindle formation
10. b) Metaphase
c) fusion
d) Non disjunction
7. In the cell cycle DNA synthesis takes place during
a) G1 phase
b) G2 phase
c) S phase
d) Prophase
8. During metaphase mitosis chromosomes
a) undergo coiling
b) Move towards the poles
c) Line up at the equator
d) Break and disintegrate
9. A cell divides every one minute. At this rate of division it can fill a 100 ml of
beaker in one hour. How much time does it take to fill a 50 ml beaker?
a) 30 minutes
b) 60 minutes
c) 59 minutes
d) one minute
10. In how many cells the meiotic division has taken place, if the total number of
spermatids produced are 32?
a) 16
b) 8
c) 32
d) 4
11. Zygotic meiosis occurs in
a) Pteris
b) Marchantia
c) Puccinia
d) Chalmydomonas
12. Daughter cells are formed as a result of meiosis are not similar to that of parent
cell because
a) Meiosis is completed in two stages
b) Prophase is longest phase
c) Nucleus size increases in daughter cells
d) Crossing over takes place and chromosome number is halved
13. During meiosis chiasmata are observed at
a) Pachytene
b) Diplotene
c) Leptotene
d) Diakinesis
14. Number of mitotic divisions required to produce 128 cells from a single cell is
a) 7
b) 8
c) 16
d) 32
15. During which stage of prophase I the crossing over takes place?
a) Pachytene
b) Leptotene
c) Zygotene
d) Diplotene
Answers
1. a) S phase
2. c) Synapsis
3. b) Spindle formation
4. a) Zygotene
5. d) one reduction division followed by one mitotic division
6. d) Non disjunction
7. c) S phase
8. c) Line up at the equator
9. c) 59 minutes
10. b) 8
11. d) Chalmydomonas
12. d) Crossing over takes place and chromosome number is halved
13. a) Pachytene
14. a) 7
15. a) Pachytene
MCQ on Plastids
1. Which of the following is a double membrane bound organelle
a) mitochondria
b) chloroplast
c) nucleus
d) all of these
2. Plastids are absent in
a) animals and plants
b) fungi and animals
c) animals, bacterium and fungi
d) none of these
3. All are colourless plastids (leucoplasts) except
a) elaioplast
b) amyloplast
c) proteinoplast
d) rhodoplast
4. Which of the following statements are incorrect regarding leucoplasts
a) leucoplasts contain thylakoids and photosynthetic pigments
b) Elaioplasts store lipids
c) proteinoplats store proteins
d) amyloplasts store starch
5. The organelle responsible for life on this planet is
a) mitochondria
b) chloroplast
c) nucleus
d) ribosomes
6. Which of the statements are true regarding chloroplast
a) It is a double membrane bound organelle
b) Chloroplasts are site of photosynthesis
c) Chloroplasts are responsible for the synthesis of carbohydrates
d) all of these
7. Chloroplast is similar to mitochondria in having a
a) double layered membrane
b) Circular DNA
c) 70S ribosomes
d) all of these
8. The site of light reaction is
a) grana
b) stroma
c) thylakoid lumen
d) outer membrane
9. Photolysis or water splitting complex is present in
a) grana
b) stroma
c) thylakoid lumen
d) outer membrane
10. Calvin cycle or dark reaction occurs in the
a) grana
b) stroma
c) thylakoid lumen
d) outer membrane
11. Which of the following pigment is most abundant in green plants
a) chlorophyll a
b) chlorophyll b
c) carotene
d) xanthophyll