CellModuleExam2feedback

Exam#2SetA
1.Thepedigreeisadiagnostictoolbecause
A.Itcanestablishthepatternofinheritanceinafamily
B.Ithelpsinidentifyingatriskfamilymembers
C.Itcanexplorethepatientslevelofunderstandingofhis/herillness
D.Alloftheabove
2.Confoundingfactorsinrecognizingpatternsofinheritanceexcept
A.Smallfamilysize
B.Nonpaternity
C.Sexinfluencedorsexlimitedgeneexpression
D.Noneoftheabove
3.Inautosomalrecessivedisorders
A.Malesandfemalesequallyaffected
B.Parentsofaffectedchildaresymptomaticcarriersofmutantalleles
C.Recurrenceriskis1in2
D.AandCarecorrect
4.Characteristicsofautosomaldominantinheritanceinclude
A.Maletofemaletransmission
B.Maletomaletransmission
C.Maleswithunaffectedoffspring
DAlloftheabove
5.InXlinkedrecessiveinheritance
A.HeterozygousFcanmanifestdisorder
B.Genenevertransmittedfathertoson
C.Carriersarefemaleswith.
D.Allarecorrect
6.Unusualinheritancepatternsareseeninthefollowing
A.Tripletrepeat
B.Mitochondrial
C.Genomicimprinting
DAlloftheabove
7.Theff.Arefactorsthatincreasetheriskofmultifactorialdisordersinrelativesexcept:
A.Probandofmorerarelyaffectedsex
B.Severeorearlyonsetofdiseaseintheproband
CLowheritabilityofthedisorder
D.Alloftheabove
8.Inmitochondrialdisorder
A.Fathernotransmit
B.Malesfemalesequallyaffectedofteninmultiplegenerations
C.Highlyvariableclinicalexpressivity
D.Alloftheabove
9.Inanautosomaldominantdisorders:
A.Variabilityoftheconditionnotseen
B.Homozygotelessaffectedthanheterozygotes
C.Conditioninonegenerationonly
D.Noneoftheabove

10.Nonmendelianinheritancepatternsareseenintheff.Except
A.Tripletrepeatexpansiondisorder
B.Mitochondrialdisorder
C.Genomicimprinting
D.Noneoftheabove
11.Tripletrepeatexpansioncharacterizedby
A.Segmentscontainingrepeatof3nucleotides
B.Individualshavethesamenumberoftriplerepeats
C.Expressionoftripletrepeatsinonegenerationandthenext
D.BothAandC
12.Imprintingrefersto:
A.Aparentoforigineffectinwhichmodificationofageneallowsittobeexpresseddifferently
B.Inactivationofimprintedcopyofthegenewhichpreventsgeneexpression
C.PraderWiliandAngelmanSyndromes
D.Alloftheabove
13.Pompesdiseaseisgenerallyrecessivemutation.Themutantfunctionassociatedwiththediseasemost
probablyresultsin
A. Lessenzyme
B. Hyperexpressionofmutant
C. Continuousproductionofenzyme
D. HigerVmax
14.in2014,UKdebatestookplaceforIVFtechnologies,particularlyinoocytemodification.Oocyte
modificationwillhelp:
A.Removeepigenetic
B.
C.AvoidmitochondrialGeneticdisorders
15.Inthepedigreediagram,afemaleindividualisrepresentedby
A. Square
B. Circle
C. Diamond
D. Noneoftheabove
16.Ifyouhavenaturalmethodofnucleicacidpolymerizationandlookedatmostrecentlyaddedmonomer,
whatwouldyoufindattheend?
A. A5OH
B. A5phosphate
C. A3OH
D. A3phosphate
17.WhichoftheffisresponsibleforseparatingthebasepairedstrandsofDNA?
A. Topoisomerase
B. Helicase
C. Primase
D. Singlestrandbindingproteins
18.WhichofthefollowingisfalseabouteukaryoticDNAreplication
A. Semiconservative
B. Semidiscontinuous
C. Percussive
D. Singleorigin
19.WhichoftheFfisresponsibleformismatcherrorcheckingprimaryreplicationmachinery

A. 53exonucleaseofDNApolymerase
B. 35exonucleaseofDNApolymerase
C. MismatchrepairofDNAligase
D. MismatchrepairofRNAseH
20.WhatistrueaboutTelomeresandtelomerase
A. Activeinmostcells
B. Leadstosinglestrandedends
C. Extendsat5end
D. Addsnonrepeatingextensivesequence
21.Whichofthefollowingenzymeshastheclosesmodeofactiontothatofatelomerase
Reversetranscriptase
22.Differencesbetweentheendofmitosisandendofmeiosis
A. Diploid:Diploid
B. Haploid:Diploid
C. Diploid:Haploid
D. Diploid:tetraploid
23.Notrepeatedinmeiosis
A.Interphase
B.Prophase
C.Anaphase
D.Telophase
24.Geneticrecombinationinmeiosisoccursataperiodwhere
A.Only1sisterchromatidperchromosome
B.2sisterchromatidsperchromosome
C.4sisterchromatidsperchromosomebutseparatepairsofdyads
D.Thereare4sisterchromatidsperchromosomeandtheyarejoinedassingletetrad
25.Whichproteinorenzymeispartofthednaprocessingmachineryassociatedwith

a.Helicase

b.rad51
c.
d.ligase

26.AlltrueforDNAandRNApolymeraseexcept
A.Bothrequiretemplate
B.bothrequireaprimer
C.Bothcatalyzereactionsthatproducepyrophosphateasoneproduct
D.Bothsynthesizeagrowingchainofnucleotideinthe5to3direction
27.Ineukaryoticcellssplicinglocationsareindicatedby:
2conservedsplicesitesandaconservedbranchsite
28.Whichoftheffistrueregardingthefunctionofdifferentclassesofribonucleicacids
A.RibosomalRNAperformsthePeptidyltransferasefunction
B.tRNAisaribsomalprotein
C.lncRNAisusedastemplateforproteinsynthesis
D.miRNAcatalysesplicing
29.FalseaboutcaponmRNA
A.Thecapisattachedtoamodifiedpurine
B.5to5triphosphatelink
C.attachedtothe5end

D.Actsascounterfortranslation
30.GiventhefollowingdoublestrandedDNAsequence,withtheupperstrandasthecodingstrand..
ACACCATGGTT
A.
B.ACACCAUGG.
C.
D.
31.Tissuespecifictranscriptionfactorsbindto
A.Promoters
B.Enhancers
C.Receptors
D.Lariats
32.Terminationoftranscriptionby
A. Stopcodon
B.SequenceencodedinDNA
C.Operators
D.PolyAtail
33.Inthestandardkaryotyping,chromosomesareidentifiedbythefollowingexcept:
Giemsastainingpattern
Sizeofchromosomes
Angleofbending
34.WhatdictateswhichpartsoftheDNAareamplifiedduringPCR
APrimers
B.Helicase
D.Topoisomerase
35.NegativecontrolofPCR

A. everythingexceptpolymerase
B. everythingexceptprimase
C. everythingexcepttemplate
D. everythingexceptdNTPs
36.IntheHHMIBacterialVirtualLab,youhavenoexactmatchtoyoursequence,butyoufinda
numberofcloselyrelatedonesfromthesamespecies.Assumingtheexperimentworked,your
bacterialsample:
A. Noconclusioncanbedrawn
B. Isprobablyafakesample
C. Isprobablyfromanentirelynew,previouslyunknowngenus
D. Isprobablyavariantofaspecies
37.Microarraysaretypicallyusedtolookat
DComparativegeneexpressionoftwocelltypes
38.Insangersequencing,sectionsofsequencewithclearreadouts
B.Middleofobtainedsequences
39.Inthegeneticengineeringexercisewhichofthefollowingisnotutilizedinplantsforinsertingnewgenes
inthem
Retroviruses
40.Thestepthatintroducesthenewgeneintothehostcelliscalled
A.Amplification
B.

C.Transformation
D.
41.ThenaturalCRISPRCas9systeminvolvedin_targets_
A.Bacteria:otherbacteria
B.Bacteria:viruses
C.Viruses:bacterialhosts
D.Viruses:otherviruses
42.IntheCRISPRCas9System,whichismodifiedtospecificallyeditparticularsegmentsofhumanor
mammaliangenomes?
A.Cas9
B.Shortpalindromicrepeat(s)
C.Thespacersequences
D.ScaffoldortracrRNA
43.AcytotoxicTcellsendsanapoptoticsignaltoavirusinfectedcellafterinspectingandrecognizingvirus
peptidesattachedtotransmembranecomplexesonthesurfaceofthevirusinfectedcell.Thisrecognition
andsignalingislikelytobevia_______signaling.
A. Endocrine
B. Paracrine
C.Contactdependent
D. Synaptic
44.Actionofsteroidsvs.Peptides
C.FormhormonereceptorcomplexthatentersnucleusactivatingmRNAtrans.
45.Epinephrineleadstoincreasedcontractionoftheheartbutincreasedbreakdownofglycogeninlive
throughthe
A. ligandbindingregionsofreceptors
B. gproteinscoupledtoreceptors
C. activatedkinases
D. activatedtranscriptionfactors
46.Thehormoneglucagonexertsitseffectbytheadenylatecyclasesystem.Whichofthefollowing
statementsistrue?
D.
47.Inresponsetotheneedforincreasedglucoseinthebloodstreamduringfightorflight,signalinginliver
D.Activatesenzymeinonepathwayanddeactivatesanotherenzymeinadifferent
pathway
48.Whichistrueofthesecondarymessengersystem?
A.CyclicAMPactivatesphosphokinaseA
B. DiacylglycerolactivatesphospholipaseC
C.InositoltriphosphateactivatesGprotein
49.Gproteins:
A.Spontaneouslyresetthemselves
50.WhichdoesViagrainhibit
51.Whichoftheffallowscelltocelltodecideonasingleaction,whentherearemorethan1typeofsignal
whichmayconflictwitheachother?
A.Signalreception
B.Signalintegration
C.SignalAmplification
D.Signalreset
52.AnewpeptidehormoneIsdiscovered.Whatislikelytrueaboutitsreceptor?

A.Itismembranebound
53.Bacteriawithhighlevelsofenzymeforlactoseevenwithglucoseandlactosepresent
D.MutationsinCAPtopreventregulation
54.Tissuespecificexpressionofgenesismediatedbythefollowingexcept
A.Differenttissecontaindifferentpromotersequencesintheirgenome
B.Differenttissueusedifferentsetsoftranscriptionfactors
C.DifferenttissueexposedifferentsectionsofDNA
D.Differenttissueusedifferentsetsofsignalingmolecules
55.ComplexthatisresponsibleformRNAmediateddownregulationofgeneexpression
A.Dicer
B.Slicer
C.RISC
D.Argonaut
56.Highgenecpynumbers=normalhighlevelexpressionof
B.Transcriptionfactorgenes
57.Constitutiveexpression
Geneisexpressedatarelativelyconstantlevel
TFnotrequiredforexpression
Expressionisproductofseveralgenes
58.Transcriptionfactorsequencespecificityisoftentoo
A.MajorGroove
59.Tamoxifenestrogenreceptormodulator
B.
60.Inhumans,apo48foundinthesmallintestineswhatdoyouLEASTexpect?
61.Whichoftheff:istrueaboutproteinsynthesis?
D.entryofaminoacyltRNAandtranslocationofribosomemakesuseofGTP
62.FirstaminoacyltRNA
B.StartsatPsite
63.Fidelityintranslationisbroughtaboutbytheffexcept
Exonucleaseactivitybytheribosome
64.TranslatethemRNAsequenceintoprotein,giventhatthesequencestartsupstreamofthestartcodon.
ACCAGAUGCUCAAACUGUCACGG
B.MetLeuLysLeuSerArg
65.Allofthefollowinginhibittranslationexcept
A.Decitabine
B.Streptomycin
C.Ricin
D.Diptheriatoxin
66.Proteinsthataretargetedtoanorganelleexceptthenucleusorforexport:
B.Signalsequence
67.Membraneproteinsaredegradedvia
A.Lysosomes
B.Peroxisomes
C.Proteasomes
D.MatrixMetalloproteases
68.Proteinstargetedfordegradationviaproteasomeareconjugatedto
A.Fmet

B.PEST
C.Glutathione
D.Ubiquitin
69.Thedecisionmakeronwhichproteinstotargetfordegradationis:
A.Thelysosome
B.Theproteasome
C.TheE3Enzymecomplex
D.Ubiquitin
70.Insulinissynthesizedaspreproinsulin.Whatposttranslationalmodificationconvertspreproinsulinto
insulin?
A.Phosphorylationofsomeaminoacids
B.Sulfationofsomeaminoavids
C.CleavageofsomeamiTnoacidsequences
D.Ubiquitination.
71.Acetylationofhistonesistypicallyassociatedwith
A.Activationofgenes
B.Silencingexpressionofgenes
C.Highleveloftranscription
D.DNArecombinationevents
72.TheDNAmethylationsitesareoftenat
A.ATG
B.CpG
C.GGG
D.ATrichregion
73.DNAmethylationisassociatedwith
B.Silencingexpressionofgenes
74.Methylationofhistonesisassociatedwith
A.Activationofgenes
B.Silencingofgenes
C/Activateorsilence,dependingonresidue
D.Activateorsilence,dependingon.?
75.Whichoftheff.RNAtypesisassociatedwithepigeneticmarksand.
IncRNA.
76.Thedrugsuberoyanilidehydroxamicacid(SAHA)isknowntoinhibithistoneacetyltransferases(HATs).
Whatmightitleadto?
A.Silencingofgenes
B.Activationofgenes.
C.Silencingoractivationofgenesdependingonwheretheacetylgroupistransferred
77.ThiscompoundisknowntodeactivateDMNT1andbeingconsideredtoimproveeffectivenessingene
cancertreatments.WhatisexpectedoftheDNMT1inhibitortreatment?
A.Increasedmethylationthroughseveralroundsofcelldivision
B.Removalofexistingmethylationpriortocelldivision
C.Inabilitytomakenewmethylationmarksinresponsetotheenvironment
D.Cellswithhalfmethylationafteroneroundofdivision
78.Epigeneticsmarksontheffgenesareerasedingametogenesisexcept:
A.Imprintedgenesfromparents
B.Nonimprintedgenesfromsexchromosomes
C.Nonimprintedgenesfromautosomalchromosomes

D.Noneoftheabove
79.Inangelmanandpraderwilisyndrome
A.Thereisadeletioninagenesubjecttoimprinting
80.DuringwhichspecificportionofinterphaseistheamountofnuclearDNAdoubled
A.G1
B.S
C.Cytokinesis
D.G2
81.Whichfeatureiscorrectaboutembryoniccellsofinsectsandseveralothernonmammaliananimalsthat
haveshorterlifecycles?
A.NoG1,shorterSphase
82.Inculturedmammalianlymphocyteswith1824hourgenerationtime,howlongdoesDNAsynthesis
usuallytake?
A.30minutes
B.23hours
C.68hours
D.1618hours
83.Whichproteinmaintainssisterchromatidattachmentbyinhibitingtheseparaseenzyme

A. Securin
B. Procaspase
C. Rb
D. Cohesin
84.IfanondegradablemitoticcyclinwasintroducedduringentryintoMphase,whichwouldhappen?
A.Inhibitionofnuclearenvelope
85.Whichproteinservesastheguardianofthegenome
Ap53
86.Whatexplainsprogeny(daughtercells)inmitosisbeinggeneticallyidentical?
A.Faithfulandtermconservativereplication
87.Apoptosisnormallyoccursintheff.Except
A.Developmentofleukemia
88.Anincreaseinp53activitypromoteswhichoftheff:
AApoptosis.
B.Continuationofthecellcycle
C.Carcinogenesis
D.Alloftheabove
89.Inthecontrolofthecellcycle,whichmechanismensuresthateverythingisreadybeforetheMphase
C.G2checkpoint
90.Acellthatdoesnotreceivesignalsfromothercellstypically
Goesintoapoptosis
91.Astemcellis

A.abletomakeanewindividual
B.abletomakenewstemcells
C.abletomakedifferentiatedcells
D.abletomakebothstemcellsanddifferentiatedcells
92.Embryonicstemcellculturesaretypicallymadefrom

a. Singlecellzygote
b. Innercellmassofblastocyst

c. Outercellmassofblastocyst
d. Umbilicalcord
93.TransdifferentiatedcellsaredifferentfromIPSbecause

Atheyaretypicallynaturallyoccurring
B.Theyrequiresomaticcell(Unabletofinish)
C.Theydonotpassbythestemcellstage
94.IntheclassicyamanakamethodofIPSgeneration,howmanygenesareusedfortheinduction?

a.
b.
c.
d.

1transcriptionfactor
4transcriptionfactors
2growthfactorreceptorsand2transcriptionfactor
4growthfactorreceptors

95.Inmammaliantissuecultureofcells,FBSprovides
A.Toprovidesignalingmolecules
B.BalanceofVitandminerals
96.InMySistersKeeper,AnnawasborntoprovideumbilicalcordbloodtohersicksisterKate.

Umbilicalcordcellsarethefollowingexcept:
a. Areconsideredmultipotent
b. Areconsideredadultstemcells
c. Arestoredorbankedinsomefacilitiesforfutureuse
d. Areusedtoregenerateallcelltypes
97.Ingeneral,theextentofDNAmethylationinpluripotentcellsis_____differentiatedcells
A.Lower
B.Thesameas
C.Higherthan
D.Isasversatileas
98.GefitinibisaselectiveEGFreceptortyrosinekinaseinhibitor.Whichhallmarkofcancerdoesit
target?
A.Selfsufficiencyingrowthsignals
B.Inflammation
C.Deregulationofcellmetabolism
D.Avoidingimmunedestruction
99.Whichofthefollowingistrue:

A. AsinglemutationcancauseCancer
B. Mutationcanbeduetodietandlifestyle
100.Whichoftheffisconsideredanenablinghallmarkofcancerinhanahanandweingbergsframework?

a.
b.
c.
d.

Inflammation
Deregulationofcellularmetabolism
Avoidingimmunedestruction
Angiogenesis

101.TheinactivationofRBproteininretinoblastomaimpairsG1checkpoint,UValsocauses
B.Melanoma
102.Whichofthefollowingdescribesthecancercellinasolidtumor

ASamegenome
BSinglecell,Theydescendfromasinglecell

CHighlydifferentiated
D(Wasntabletocopy)
103.Acompoundderivativefrommangosteenisaninhibitorof

ASelfsufficiencyingrowthsignals
BEvasionofapoptosis
CInvasionandmetastasis
Dangiogenesis
104.Breastcancerisnotalwaysheridatarybecauseitisusually
A.Germline
D.Somatic
105.PalbociclibisadrugforthetreatmentofER(estrogenreceptor)positiveandHER2negative

breastcancerinpostmenopausalwomen,developedbyPfizer.Itisaselectiveinhibitorofthe
cyclindependentkinasesCDK4andCDK6.Whichhallmarkerdoesittarget?
D.Insensitivitytoantigrowthsignals
106.InWeinbergslectureonstemcellsandcancercellsheshows:

AForstemcellstoundergometastasistheyhavetoundergoepithelialtomesenchymal
transition
BAsmallfractionoftumourcellsareabletoformnewtumours
107.Whenacancercellleavesitspositionithasto:
A.Epithelialmesenchymaltransition
108.Extensiveprimarycellmorphologicalandspatialrearrangementscanbeseeninthe

AAnimalPole
BVegetalPole
COutercellmembrane
DInnercellmass
109.Somitesareformedfromtissuederivedfrom

Aectoderm
Bendoderm
Cmesoderm
Dblastocoel
110.Whichofthefollowingorganismsisnotusedinmodelinghumandisease
AArabidopsisthaliana
BDrosophilamelanogaster
CDaniorerio
DRattusnorvegicus
111.Whatmakeszebrafishanattractivevertebratemodelfordevelopmentalbiologyandfor
humandiseaseresearch?
Aeasytobreedandpropagate
Blarvaearesmallandopticallytransparent
CHas>75%geneticsimilaritytohumans
DAlloftheAbove
112.Geneknockdownsinzebrafishareoftendoneusing

AsiRNA
Bmorpholino

CCRISPR
DAlloftheabove
113.Geneknockoutsinzebrafishareoftendoneusing
A.siRNA
B.Morpholino
C.CRISPR
D.Alloftheabove
114.HowdomorpholinosinterferewithmRNA
A. Blocktranslation
B. Causemissplicing
C. BothAandB
D. NeitherAorB
115.InCRISPRtechnology,whatproteinsserveasthemolecularscissors?
A. Cas9
B. Meganuclease
C. EndoR
D. Noneoftheabove
116.Whichnitrogenousbaseismostsusceptibletodeamination?
A.Uracil
B.Cytosine
C.Adenine
D.Thymine
117.WhichoftheffcharacteristicsofDNAserveasbasisforfidelityinreplication
Complementarityofnucleotidebases
118.Inthesicklecellanemia,singlenucleotidemutationonthegeneleadsto.Whattypeofmutationis
showcased?
B.Missense
119.Whichoftheff.isexpecteddangerinsinglebaseinsertionordeletion
A.Frameshiftmutation
B.Silentmutation
C.Partiallyacceptablemissensemutation
D.Unacceptablemissensemutation
120.Sunlightsensitivityandsusceptibilitytoskincancerofpersonwithxerodermapigmentosumisdueto
deficientactivitiesofwhichDNArepairsystem?
A. Baseexcisionrepairsystems
B. Doublestrandbreakrepairsystems
C. Mismatchrepairsystems
D. Nucleotideexcisionrepairsystem
121.Geneticinfocanbeexchangedbetweensimilarchromosome.Recombinationoccursduring
B.ProphaseI
C.ProphaseII
122.Whatisthegeneticconsequenceofexchangeofgeneticinformationbetweentwogeneticallyidentical
sisterchromatids,assumingtheexchangeistheresultofanequalcrossover?
A. None
B. Deletion
C. Polyploidy
D. Chromosomaltranslocation

123.Inrepairingamismatchstrandthatescapedproofreading,whichenzymesareutilized
DNApol1andLigase
124.Thefllowingarecorrectfeaturesofhereditarynon=polyposiscoloncancerorlynchexcept
A.Familialclustering
B.Occurinpatientsabove60
C.Locatedinrightcolon
D.Defectinmismatchrepairandmicrosatinstability
125.AnexperimentalcellwasexposedtoUVlighttoinducemutation.Whatisthegeneticeffectofthymine
dimersinDNA?
PreventionofDNAreplicationbeyondDimer
126.THEBRCA1geneoritsgeneproduct
A.IslocatedontheXchromosome
B.Isaubiquitinproteinligase
C.IsareceptorTyrosinekinase
D.Isaprotooncogene
127.CancersusceptibilitygenessuchasBRCA1areexamplesof
A.XlinkedDisease
B.Singlegeneautosomaldominantdisease
C.Singlegeneautosomalrecessivedisease
D.Multifactorial
128.WhatisthekeyBRCA1mutationassociatedwithcancerinAshkenaziJews?
Insertion/deletions(indels)
129.BRCA1mutationsassociatedwith
A.BreastCancer
B.BreastandOvariancancers
C.Breast,ovarian,cervical,uterineandfallopiancancers
D.Manycancers
130.YoucouldnormallyrecommendBRCA1or2screening,ifavailable,forthefollowingexcept:
A.Someonewhohasafirstthirddegreerelativewithanykindofcancer