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Abstract
Amylase enzyme (endo--1,4-glucan glucanohydrolase, EC. 3.2.1.1) is an enzyme that catalyzes
the hydrolysis reaction. Amylase changes carbohydrates (polysaccharides) into maltose (amilase dan -amilase) or glucose (glucoamylase). Amylase enzyme can be found in sweet
potato. The purpose of this experiment is to determine and calculate the kinetics of amylase
enzyme in sweet potato extract. The determination of Vmax and Km is to determine the
characteristic of enzyme. Vmax is the limit of increasing enzyme which is enzymes activity reach
its maximum speed. Km is the constants value of substrate (S) which is the speed of enzyme
reaction berubah menjadi of Vmax. The method to calculate the value of Km and Vmax can be
used by DNS reagent. The DNS method is used to determine total reducing sugars in the sample
that contain carbohydrates. This observation used with variant of starch substrates and
incubation time. DNS methods used spectrophotometer to measuring the absorbance of the
sample with a wavelength 540 nm. The relation between addition of concentration with time and
the speed is directly proportional, it shows that any increase in the concentration, the speed will
increase as well. Based on the Lineweaver-Burk graph, the value of Km got 2.64% and Vmax
0.0197 mg/ml minute.