Cell line

Meaning

Organism

Origin tissue

Morph

"3-day transfer,
3T3-L1

inoculum 3 x 10^5

Mouse

Embryo

Fibroblast

4T1

Mouse

Mammary gland

9L

Rat

Brain

Glioblastoma

A172

Human

Brain

Glioblastoma

A20

Mouse

B lymphoma

B lymphocyte

A253

Human

Submandibular duct

Head and nec

A2780

Human

Ovary

Ovarian carci

A2780ADR

Human

Ovary

A2780cis

Human

Ovary

A431

Human

Skin epithelium

Squamous ce

A549

Human

Lung

Lung carcinom

AB9

Zebrafish

Fin

Fibroblast

cells"

Adriamycin-re

derivative of A

Cisplatin-resis
of A2780

Cell line

AHL-1

Meaning

Armenian Hamster

Organism

Origin tissue

Morph

Hamster

Lung

ALC

Mouse

Bone marrow

B16

Mouse

Melanoma

B35

Rat

Neuroblastoma

BCP-1

Human

PBMC

Human

Lung

Epithelial

Mouse

Brain/cerebral cortex

Endothelium

Hamster

Kidney

Fibroblast

Human

Kidney (embryonic)

Epithelium

Human

Breast epithelium

Breast carcino

Lung-1

Stroma

HIV+ primary
lymphoma

Bronchial epithelium +
BEAS-2B

Adenovirus 12-SV40
virus hybrid
(Ad12SV40)

bEnd.3

BHK-21

BOSC23

BT-20

Brain Endothelial 3

Baby Hamster Kidney21

Packaging cell line

derived fromHEK 293

Breast Tumor-20

Cell line

Meaning

Organism

Biopsy xenograft of
BxPC3

Pancreatic Carcinoma

Human

line 3

C2C12

Mouse

C3H-10T1/2

Mouse

C6

Rat

Insect - Asian tiger

C6/36

mosquito

Origin tissue

Pancreatic
adenocarcinoma

Morph

Epithelial

Myoblast

Embryonic
mesenchymal cell line

Brain astrocyte

Glioma

Larval tissue

Caco-2

Human

Colon

Colorectal car

Cal-27

Human

Tongue

Squamous ce

CGR8

Mouse

Embryonic stem cells

Hamster

Ovary

Epithelium

Human

CML acute phase

T cell leukem

Dog

Mammary gland

Epithelium

CHO

Chinese Hamster
Ovary

Chronic myeloid
CML T1

leukemia T lymphocyte
1

CMT12

Canine Mammary
Tumor 12

Cell line

Meaning

Organism

Origin tissue

Morph

COR-L23

Human

Lung

Lung carcinom

COR-L23/5010

Human

Lung

Lung carcinom

COR-L23/CPR

Human

Lung

Lung carcinom

COR-L23/R23-

Human

Lung

Lung carcinom

Kidney

Fibroblast

COS-7

Cercopithecus

Old World monkey

aethiops, origin-

-Cercopithecus

defective SV-40

aethiops(Chlorocebus)

Ovarian granu

COV-434

Human

Ovary

CT26

Mouse

Colon

Colorectal car

D17

Dog

Lung metastasis

Osteosarcom

DH82

Dog

Histiocytosis

Monocyte/ma

DU145

Human

DuCaP

E14Tg2a

Dura mater cancer of

the Prostate

Human

Mouse

carcinoma

Androgen insensitive
prostate carcinoma

Metastatic prostate
carcinoma

Epithelial

Embryonic ste

Cell line

Meaning

Organism

Origin tissue

Morph

EL4

Mouse

EM-2

Human

CML blast crisis

Ph+ CML line

EM-3

Human

CML blast crisis

Ph+ CML line

EMT6/AR1

Mouse

Mammary gland

Epithelial-like

EMT6/AR10.0

Mouse

Mammary gland

Epithelial-like

FM3

Human

Lymph node metastasis

Melanoma

Mouse

Brain

Glioma

H1299

Human

Lung

Lung carcinom

HaCaT

Human

Skin

Keratinocyte

HCA2

Human

Colon

Adenocarcino

Human

Kidney (embryonic)

Epithelium

GL261

HEK 293

Glioma 261

Human Embryonic
Kidney 293

T cell leukem

HEK 293T

HEK 293 derivative

Human

Kidney (embryonic)

Epithelium

HeLa

"Henrietta Lacks"

Human

Cervix epithelium

Cervical carci

Cell line

Hepa1c1c7

Meaning

Clone 7 of clone 1
hepatoma line 1

Hep G2

Morph

Hepatoma

Epithelial

Human

Liver

Hepatoblasto

- Trichoplusia ni

Human Leukemia-60

Origin tissue

Mouse

Insect (moth)

High Five

HL-60

Organism

Human

Ovary

Blood

Myeloblast

HT-1080

Human

Fibrosarcoma

HT-29

Human

Colon epithelium

Adenocarcino

J558L

Mouse

Myeloma

B lymphocyte

Jurkat

Human

White blood cells

T cell leukem

JY

Human

Lymphoblastoid

EBV-transform

K562

Human

Lymphoblastoid

CML blast cris

KBM-7

Human

Lymphoblastoid

CML blast cris

KCL-22

Human

Lymphoblastoid

CML

KG1

Human

Lymphoblastoid

AML

Cell line

Meaning

Ku812

Organism

Origin tissue

Morph

Human

Lymphoblastoid

Erythroleukem

Human

Lymphoblastoid

CML

L1210

Mouse

Lymphocytic leukemia

Ascitic fluid

L243

Mouse

Hybridoma

KYO-1

LNCaP

Kyoto-1

Lymph Node Cancer of

the Prostate

Human

Prostatic
adenocarcinoma

Secretes L24
HLA-DR)

Epithelial

Secretes MA2
MA2.1

Mouse

Hybridoma

(against HLAB17)

Ma-Mel 1, 2,
3....48

MC-38

MCF-7

MCF-10A

MDA-MB-157

Mouse Colon-38

Michigan Cancer
Foundation-7

Michigan Cancer
Foundation-10A

M.D. Anderson Metastatic Breast-157

Human

Skin

Mouse

Colon

Human

Breast

Human

Breast epithelium

Human

Pleural effusion
metastasis

A range of me
lines

Adenocarcino

Invasive brea

carcinoma ER

Breast carcino

Cell line

MDA-MB-231

MDA-MB-361

MDA-MB-468

MDCK II

Meaning

M.D. Anderson Metastatic Breast-231

M.D. Anderson Metastatic Breast-361

M.D. Anderson Metastatic Breast-468

Madin Darby Canine

Organism

Human

Origin tissue

Pleural effusion
metastasis

Breast carcino

Melanoma (co

Human

Human

Morph

M14)

Pleural effusion
metastasis

Breast carcino

Dog

Kidney

Epithelium

MG63

Human

Bone

Osteosarcom

MOR/0.2R

Human

Lung

Lung carcinom

Mono-Mac-6

Human

White blood cells

Myeloid meta

Human

Lung (fetal)

Fibroblast

MRC-5

Kidney II

Medical Research
Council cell strain 5

MTD-1A

MyEnd

Myocardial Endothelial

Mouse

Epithelium

Mouse

Endothelium

NCI-H69

Human

Lung

Lung carcinom

NCI-H69/CPR

Human

Lung

Lung carcinom

Cell line

Meaning

Organism

Origin tissue

Morph

NCI-H69/LX10

Human

Lung

Lung carcinom

NCI-H69/LX20

Human

Lung

Lung carcinom

NCI-H69/LX4

Human

Lung

Lung carcinom

Neuro-2a

Mouse

Nerve/neuroblastoma

Neuronal stem

Mouse

Embryo

Fibroblast

NALM-1

Human

Peripheral blood

Blast-crisis CM

Neuro2a

Mouse

Nerve/neuroblastoma

Neuronal stem

NK-92

Human

Leukemia/lymphoma

NTERA-2

Human

Lung metastasis

Embryonal ca

NW-145

Human

Skin

Melanoma

NIH-3T3

NIH, 3-day transfer,

inoculum 3 x 105 cells

Virginia
OK

Opossum Kidney

opossum - Didelphis

Kidney

virginiana

OPCN / OPCT
cell lines

Human

Prostate

Range of pros
lines

Cell line

Meaning

Organism

Origin tissue

Morph

P3X63Ag8

Mouse

Myeloma

PC12

Rat

Adrenal medulla

Pheochromoc

Human

Bone metastasis

Prostate carc

Peer

Human

T cell leukemia

PNT1A

Human

Prostate

PNT2

Human

Prostate

PC-3

Prostate Cancer-3

The second cell line

Pt K2

derived fromPotorous

Long-nosed potoroo -

SV40-transfor
line

SV40-transfor
line

Kidney

Epithelial

Human

B lymphoma

Lymphoblast-

Rat

Leukemia

Basophil cell

Mouse

Kidney

Renal carcino

RIN-5F

Mouse

Pancreas

RMA-S

Mouse

tridactylis

Raji

RBL-1

RenCa

Rat Basophilic
Leukemia-1

Renal Carcinoma

Potorous tridactylus

T cell tumour

Cell line

Meaning

Late stage (2024

melanogaster

hours old) embryos

Sarcoma OSteogenic-2

Human

Bone

Spodoptera

Insect (moth)

frugiperda 21

- Spodoptera frugiperda

Spodoptera

Insect (moth)

frugiperda 9

- Spodoptera frugiperda

Schneider 2

SaOS-2

Sf9

Origin tissue

Insect - Drosophila

S2

Sf21

Organism

Morph

Osteosarcom

Ovary

Ovary

Bone marrow

SH-SY5Y

Human

SiHa

Human

Cervix epithelium

Cervical carci

Human

Breast

Breast carcino

Human

Ovary

Ovarian carci

SK-BR-3

SK-OV-3

Sloan-Kettering Breast
cancer 3

Sloan-Kettering Ovaria
n cancer 3

metastasis

Neuroblastom

T cell leukem

T2

Human

T-47D

Human

Breast

Breast ductal

T84

Human

Lung metastasis

Colorectal car

linehybridoma

Cell line

Meaning

Organism

T98G

Human

THP-1

Human

U373

Human

U87

Human

U937

Human

VCaP

Vero

Vertebral Cancer of the

Prostate

Vero (truth)

Human

African green monkey

-Chlorocebus sabaeus

Origin tissue

Glioblastomaastrocytoma

Monocyte

Glioblastomaastrocytoma

Glioblastomaastrocytoma

Morph

Epithelium

Acute monocy

Epithelium

Epithelial-like

Leukemic monocytic
lymphoma

Vertebra metastasis

Prostate carc

Kidney epithelium

VG-1

Human

Primary effus

WM39

Human

Skin

WT-49

Human

Lymphoblastoid

YAC-1

Mouse

Lymphoma

Melanoma

Cell line

YAR

Meaning

Organism

Human

Origin tissue

Lymphoblastoid

See also[edit]
Mammalian Cell Culture Cell Line Cell Type Species Tissue Medium* 293
fibroblast human embryonic kidney MEM and 10% FBS 3T6 fibroblast mouse
embryo DMEM, 10% FBS A549 epithelial human lung carcinoma F-12K, 10% FBS
A9 fibroblast mouse connective tissue DMEM, 10% FBS AtT-20 epithelial mouse
pituitary tumor F-10, 15% horse serum, and 2.5% FBS BALB/3T3 fibroblast mouse
embryo DMEM, 10% FBS BHK-21 fibroblast hamster kidney GMEM, 10% FBS, or
MEM, 10% FBS, and NEAA BHL-100 epithelial human breast McCoy'5A, 10% FBS
BT fibroblast bovine turbinate cells MEM, 10% FBS, and NEAA Caco-2 epithelial
human colon adeno carcinoma MEM, 20% FBS, and NEAA Chang epithelial
human liver BME, 10% calf serum CHO-K1 epithelial hamster ovary F-12, 10%
FBS Clone 9 epithelial rat liver F-12K, 10% FBS Clone M-3 epithelial mouse
melanoma F-10, 15% horse serum, and 2.5% FBS COS-1, COS-3, COS-7 fibroblast
monkey kidney DMEM, 10% FBS CRFK epithelial cat kidney MEM, 10% FBS, and
NEAA CV-1 fibroblast monkey kidney MEM, 10% FBS D-17 epithelial dog
osteosarcoma MEM, 10% FBS, and NEAA Daudi lymphoblast human blood from a
lymphoma patient RPMI-1640, 10% FBS GH1, GH3 epithelial rat pituitary tumor F10, 15% horse serum, and 2.5% FBS * BME: Basal Medium Eagle; DMEM:
Dulbeccos Modified Eagle Medium; FBS: Fetal Bovine Serum; GMEM: Glasgow
Minimum Essential Medium; IMDM: Iscoves Modified Dulbeccos Medium; MEM:
Minimum Essential Medium; NEAA: Non-Essential Amino Acids Solution. Cell
Culture Basics | 29 Part 4. Methods Mammalian Cell Culture, continued Cell Line
Cell Type Species Tissue Medium* H9 lymphoblast human T-cell lymphoma RPMI1640, 20% FBS HaK epithelial hamster kidney BME, 10% calf serum HCT-15
epithelial human colorectal adenocarcinoma RPMI-1640, 10% FBS HeLa epithelial
human cervix carcinoma MEM, 10% FBS, and NEAA (in suspension, S-MEM) HEp-2
epithelial human larynx carcinoma MEM, 10% FBS HL-60 lymphoblast human
promyeolocytic leukemia RPMI-1640, 20% FBS HT-1080 epithelial human
fibrosarcoma MEM, 10% HI FBS, and NEAA HT-29 epithelial human colon
adenocarcinoma McCoy's 5A, 10% FBS HUVEC endothelial human umbilical cord
F-12K, 10% FBS, and 100 g/mL heparin I-10 epithelial mouse testicular tumor F10, 15% horse serum, and 2.5% FBS IM-9 lymphoblast human marrow from
myeloma patient RPMI-1640, 10% FBS JEG-2 epithelial human choriocarcinoma
MEM, 10% FBS Jensen fibroblast rat sarcoma McCoy's 5A, 5% FBS Jurkat
lymphoblast human lymphoma RPMI-1640, 10% FBS K-562 lymphoblast human
myelogenous leukemia RPMI-1640, 10% FBS KB epithelial human oral carcinoma
MEM, 10% FBS, and NEAA KG-1 myeloblast human marrow from erythroleukemia
patient IMDM, 20% FBS L2 epithelial rat lung F-12K, 10%FBS LLC-WRC 256
epithelial rat carcinoma Medium 199, 5% horse serum McCoy fibroblast mouse
unknown MEM, 10% FBS MCF7 epithelial human breast adenocarcinoma MEM,
10% FBS, NEAA, and 10 g/mL insulin WI-38 epithelial human embryonic lung

Morph

EBV-transform

BME, 10% FBS WISH epithelial human amnion BME, 10% FBS XC epithelial rat
sarcoma MEM, 10% FBS, and NEAA Y-1 epithelial mouse tumor of adrenal F-10,
15% horse serum, and 2.5% FBS Insect Cell Culture Sf9, Sf21 fall army worm
(Spodoptera frugiperda) pupal ovary TNM-FH and 10% FBS, or Sf-900 II SFM
(serumfree), or Sf-900 III SFM (serum-free) High Five (BTI-TN-5B1-4) cabbage
looper (Trichoplusia ni) ovary TNM-FH and 10% FBS, or Express Five SFM
(serum-free) Schneider 2 (S2), D.Mel-2 fruit fly (Drosophila melanogaster)
Schneiders Drosophila medium and 10% heatinactivated FBS * BME: Basal
Medium Eagle; DMEM: Dulbeccos Modified Eagle Medium; FBS: Fetal Bovine
Serum; GMEM: Glasgow Minimum Essential Medium; IMDM: Iscoves Modified
Dulbeccos Medium; MEM: Minimum Essential Medium; NEAA: Non-Essential
Amino Acids Solution; TNM-FH: Trichoplusia ni Medium-Formulation Hink (i.e.,
Graces Insect Medium, Supplemented).

Types of Cell Lines:

Finite Cell Lines :
The cells in culture divide only a limited number of times, before
their growth rate declines and they eventually die. The cell lines
with limited culture life spans are referred to as finite cell lines. The
cells normally divide 20 to 100 times (i.e. is 20-100 population
doublings) before extinction. The actual number of doublings
depends on the species, cell lineage differences, culture conditions
etc. The human cells generally divide 50-100 times, while murine
cells divide 30-50 times before dying.
Continuous Cell Lines :
A few cells in culture may acquire a different morphology and get
altered. Such cells are capable of growing faster resulting in an
independent culture. The progeny derived from these altered cells
has unlimited life (unlike the cell strains from which they
originated). They are designated as continuous cell lines.
The continuous cell lines are transformed, immortal and
tumorigenic. The transformed cells for continuous cell lines may be
obtained from normal primary cell cultures (or cells strains) by
treating them with chemical carcinogens or by infecting with

oncogenic viruses. In the Table. 36.1, the different properties of

finite cell lines and continuous cell lines are compared.