Professional Documents
Culture Documents
doi: 10.1111/j.1365-2036.2005.02645.x
SUMMARY
The peritoneum is one of the most common extrapulmonary sites of tuberculous infection. Peritoneal tuberculosis remains a significant problem in parts of the world
where tuberculosis is prevalent. Increasing population
migration, usage of more potent immunosuppressant
therapy and the acquired immunodeficiency syndrome
epidemic has contributed to a resurgence of this disease in
regions where it had previously been largely controlled.
Tuberculous peritonitis frequently complicates patients
with underlying end-stage renal or liver disease that
further adds to the diagnostic difficulty.
The diagnosis of this disease, however, remains a
challenge because of its insidious nature, the variability
of its presentation and the limitations of available
diagnostic tests. A high index of suspicion is needed
whenever confronted with unexplained ascites,
particularly in high-risk patients.
INTRODUCTION
686
Variable methods have been used to study TB worldwide and this might explain the discrepancies of data
available. Increasing migration and also the constant
changes of disease pattern are other factors that made
accurate assessments of the extent of this disease even
more complex. Examples of the methods used to study
TB prevalence include, mortality data, tuberculin skin
test and field surveillance by chest X-ray and sputum
cultures. BCG vaccination has limited the usefulness of
tuberculin skin testing in the diagnosis of TB because a
significant proportion of vaccinated but uninfected
patients will return a positive skin reaction.18 Similarly,
deriving meaningful epidemiological data on this disease by Mantoux skin testing has also become difficult
because of the vaccination programmes undertaken in
the third world. This trend is observed across all age
groups where non-vaccinated subjects had lower rates
of positive Mantoux test in uninfected patients than
those who had received BCG vaccination.18 The peak
prevalence of a positive skin reaction in those who had
previously been vaccinated occurs in 70% for the age
groups between 45 and 64 years. It is expected that the
rate of positive skin test for future generations will be
significantly higher as current immunization programmes in most of the third world countries include
BCG vaccination at child-birth. The newly developed
interferon (IFN)-c-based test would help overcome this
problem of detecting latent infection since previous BCG
vaccination does not affect its results.19 However, at
present there is no large-scale epidemiological data
available utilizing this test.
A significant correlation exists between the socioeconomic status and disease prevalence. Poor hygiene and
overcrowding have consistently been shown to have a
causative relationship with TB. Ingestion of unpasteurized milk might also be another reason for the
increased prevalence of this disease in the rural
populations. On the contrary, the effective tuberculin
testing of dairy herds and a shift from the norm of
drinking unpasteurized milk in cities, has most likely
contributed to the overall reduction in cases of primary
abdominal TB.
Alcoholic liver disease (ALD) is frequently linked to
increased incidence of TBP particularly in the western
countries. In one study, 62% of patients with TBP had
an underlying ALD,10 a contrast to the findings of
studies from developing countries where the reported
underlying liver diseases was found in <13% of patients
with TBP.2023
2005 Blackwell Publishing Ltd, Aliment Pharmacol Ther 22, 685700
687
688
Table 1. Cumulative data of clinical features compiled from 35 studies of tuberculous peritonitis (TBP)
Clinical feature
Number of cases
Average (%)
Abdominal pain
Fever
Weight loss
Diarrhoea
Constipation
Ascites
Abdominaltenderness
Hepatomegaly
Splenomegaly
12841, 7, 1113, 15, 2023, 3941, 44, 45, 47, 51, 52, 60, 94104
13931, 7, 1013, 2023, 40, 41, 44, 45, 47, 51, 52, 60, 94105
7741, 7, 1013, 2023, 3941, 51, 52, 9599, 106
6301, 7, 11, 12, 21, 23, 40, 94, 96, 98, 104
31911, 12, 21, 40, 47
14051, 7, 1012, 15, 2023, 39, 40, 41, 44, 45, 47, 51, 52, 94105,
3297, 11, 12, 20, 22, 40, 9496
3197, 1012, 20, 39, 40, 95, 103
18910, 11, 39, 40, 52, 103
64.5
59
61
21.4
11
73
47.7
28.2
14.3
include weight loss, anorexia and malaise. The superimposition of this illness on other chronic conditionslike uraemia, cirrhosis and AIDS make these symptoms
more difficult to quantify. Weight loss is seen in about
61% of cases and investigators have reported reversibility of this manifestation as one of the markers of
disease resolution.7
Abdominal pain is a common presenting symptom and
frequently accompanied by abdominal distension. It is
usually non-localized and vague in nature. The pain is
largely due to the tuberculous inflammation of the
peritoneum and mesentery. Less often, it could be a
manifestation of intermittent subacute intestinal
obstruction, a result of matted bowel loops caused by
adhesions of the mesentery and omentum. The matted
bowel loops could be felt as palpable masses on
abdominal examination. Abdominal symptoms such as
vomiting, diarrhoea and constipation are uncommon.
The pathophysiology of diarrhoea is unknown and it is
very unlikely to be due to direct intestinal involvement
as TBP rarely occurs simultaneously with tuberculous
enteritis.1, 11, 41 Khuroo and Khuroo42 suggested that
the stagnation in dilated segments of intestinal loops
caused by adhesions between mesentery and small
bowel may result in small bowel bacterial overgrowth.
The evidence for this plausible mechanism is however,
not yet available.
Tenderness on palpation is common in TBP and occurs
in almost 48% of the cases and this might help in
differentiating it from spontaneous bacterial peritonitis, which complicates portal hypertensive ascites.43
Rebound tenderness is rare as the presence of ascitic
fluid prevents approximation of the parietal with the
visceral peritoneum.
Ascites is the predominant finding and it is present in
about 73% of the patients. A smaller percentage of
107
689
Table 2. Sensitivity patterns of various diagnostic tests from the cumulative data of 39 studies of TBP
Diagnostic
test
Sensitivity
(%)
Remarks
Abnormal CXR
38
Positive PPD
53.16
Ascitic fluid tests
Predominant lymph 68.34
LDH
ADA (>30 U/L)
Smear
Culture
Laparoscopy
Visual diagnosis
Histology
77
94
2.93
34.75
92.7
93
1002 patients with TBP1, 1013, 2022, 3941, 44, 45, 47, 51, 52, 60, 9496, 98101
380 patients studied;10, 11, 2022, 39, 40, 51, 52, 96 authors used various induration sizes for positivity
477 patients;10, 11, 13, 20, 23, 40, 41, 44, 45, 47, 51, 61, 96 investigators had differing definitions
for predominance
87 patients;10, 11, 51, 52 investigators used varying LDH range
1305 patients studied; 205 with TBP11, 39, 61, 62, 108114 Hillebrand et al.61 used >7 U/L as cut-off
615 patients1, 1013, 2023, 41, 47, 51, 52, 95, 100, 101, 103, 104, 114118
446 patients;1, 1013, 20, 22, 23, 51, 52, 85, 95, 96, 99101, 103, 104, 114116, 118 studies using
BACTEC radiometric system not included
397 patients;10,
402 patients;10,
TBP, tuberculous peritonitis; CXR, chest X-ray; LDH, lactate dehydrogenase; ADA, adenosine deaminase.
2005 Blackwell Publishing Ltd, Aliment Pharmacol Ther 22, 685700
690
CA-125 levels paralleling clinical response and resolution of ascites. Based on the available evidence, this
test does not seem to offer any particular advantage
in the diagnosis of TBP.
Microbiological diagnosis
ZiehlNeelsen (ZN) staining of the ascitic fluid for
mycobacterial detection is positive in only about 3% of
cases with proven TBP (Table 2). To allow for the
detection of mycobacteria in stained smears, presence of
at least 5000 bacilli/mL of specimen is required,
whereas for positive culture as few as 10 organisms
might be sufficient for the diagnosis.58, 59 The current
gold standard for the diagnosis of TB entails culturing
the mycobacteria from clinical specimens. Culture
methods based on a combination of liquid or biphasic
media, together with solid media, are used to ensure
maximum sensitivity of detection. Studies reporting on
the microbiological diagnosis have used varied reporting methodology. While most have reported on the
regular method of culturing 1050 mL of ascitic fluid,
others have recommended culturing 1 L of centrifuged
fluid. This is based on the above principle of increasing
yield by increasing the concentration of the bacilli.
Culture of the fluid by the regular method is positive in
35% based on our cumulative data comprising of 446
patients from 22 case series (Table 2), although the
yield has been shown to significantly improve (6683%)
when 1 L of fluid is centrifuged and then cultured,
either by traditional culture media or the BACTEC
system.41, 47, 60 In clinical practice this is not practical
as the biggest available aliquots for centrifugation have
a capacity of 50 mL. This problem is further compounded by the 48 weeks requirement by the conventional
culture media. The recently introduced BACTEC radiometric system is a rapid method for detecting mycobacteria in clinical specimens, with a mean time to
detection of 14 days, and can be used to complement
conventional methods.47 A further 7 days are required
for drug susceptibility testing. Other newer methods of
identifying mycobacteria isolates include liquid chromatography and DNA probes for which the typical
turnaround time for confirmation is 21 days. Although,
the concept of culturing a litre of centrifuged ascitic fluid
is attractive, it may not be feasible in the regular clinical
setting. Hence, at least in resource-rich settings, the
BACTEC radiometric system should be made available
in routine clinical practice.
2005 Blackwell Publishing Ltd, Aliment Pharmacol Ther 22, 685700
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Figure 2. Laparoscopic image of an 18year-old girl with fever and ascites showing
multiple whitish nodules (<5 mm) covering
the peritoneum. The biopsy was consistent
with caseating granuloma and acid-fast
bacilli (courtesy: N. Azzam and
A. K. Al-Aska).
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Ascites absent
SAAG 11 g/L
Neutrophil
dominant
Imaging Studies
Ascitic fluid
analysis
Lymphocyte
dominant
Spontaneous bacterial
peritonitis Rx
Peritoneal
disease (+)
Peritoneal
disease (-)
TB culture
negative
Laparotomy
Anti-TB
treatment
Malignant
cells positive
Appearance
suggestive;
histology
inconclusive
Histology
inconclusive
Appearance
suggestive;
histology
conclusive
Laparoscopy
696
CONCLUSION
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