VIII.

TABLE OF EXPERIMENT
No
Procedure
1. Effect of pH With Enzyme Activity
saliva
diluted 10x with aquadest

Result of Experiment
Before:
Saliva = colorless
Aquadest = colorless
After:
- Saliva + aquadest diluted 10x

1 mL starch solution 1%
entered into test tube
left until 2 minutes with each temperature
added 10 drops of iodine solution
added 6 mL of aquadest
read the absorbant (A) with wavelength
680 nm
absorbant

test (U)
1 mL starch solution
with various pH
(1,3,5,7,9)

Conclusion
Based on the enzyme

about pH7 (netral)

activity enzyme

and the medium into

amylase have pH

so reactive /more

ptimum 5-7

alkalyst, enzym to

Prove based on color

Before:
Stach solution 1% :

be inactivacy,

changes on pH 7 more

enzymes on the

concentrated and

colorless
Aquadest : colorless
Iodine : yellow
After:
Starch solution + iodine +

body occur

absorbant result is

maximum activity

lowest than other

between pH 5.0-9.0
(hafiz Soewoto,

0.028

= colorless

saliva solution
blanko (B)

Hypothesis/Reaction
pH optimum enzym

aquadest : yellow darkness

After test using UV-VIS
- Blanco : 0.083

Before:
pH 1: colorless
pH 3: colorless
pH 5 : colorless
pH 7 : colorless
pH 9 : colorless
enzym solution : colorless

2000)

 entered into test tube

left until 2 minutes with each
temperature
added 10 drops of iodine solution
added 6 mL of aquadest
read the absorbant (A) with wavelength
680 nm
absorbant
Calculated dispute of absorbant
between test tube B and U from
each temperature

2.

iodine solution : yellow

After:
After + enzym solution :
- pH 1: colorless
- pH 3: colorless
- pH 5 : colorless
- pH 7 : colorless
- pH 9 : colorless
After + iodine :
- pH 1: yellow (++)
- pH 3: yellow (++)
- pH 5 : yellow (++)
- pH 7 : yellow
- pH 9 : yellow

After + aquadest :
- pH 1: light yellow (++)
- pH 3: light yellow (++)
- pH 5 : light yellow (++)
- pH 7 : light yellow
A
- pH 9 : light yellow
After test using UV-VIS
- pH 1: 0.103;A: 0.02
- pH 3: 0.066;A: 0.017
- pH 5: 0.099;A: 0.016
- pH 7: 0.055;A: 0.028
- pH 9; 0.083;A: 0
Effect of Enzymes Concentration With Enzyme Before:
Saliva = colorless
Activity
Aquadest = colorless
Strach solution 1% =
saliva
diluted 10x, 20x, 30x, 40x, 50x with
aquadest

The improvement of

Concentration affect

enzym concentration with enzyme activity,

will increase the

more higher

speed of enzymatic

concentration of

reaction. So the

enzymes, the activity

speed of enzymatic

of enzymes higher too.

reaction linier with

Enzymes amylase

enzymes

working optimum on

solution) : colorless
Tube 2(starch

concentration, if

high concentration its

concentration

10x, it proven with

solution) : colorless
Tube 3(starch

enzyme higher so

value of absorbant it is

the reaction more

0.03

entered into test tube 1,2,3,4,5

solution) : colorless
Tube 4(starch

left until minimum 2 minutes

solution) : colorless
Tube 5(starch

solution) : colorless
After +

saliva solution

colorless
I2 = yellow(+++)

After:
Tube 1(starch

Test (U)
1 mL starch solution 1%

added 10 drops of aquadest

added 0,5 mL diluted enzymes 10x
50x

starch+aquadest+enzym
e 10-50x + iodine :

shaken and left until 1 minutes

added 10 drops of iodine solution (with
temperature 60C out of steam bath)
added 6 mL of aquadest
read the absorbant (A) with wavelength
680 nm
absorba
nt
Calculated dispute of absorbant between
test tube B and U from each
temperature

Tube 1(10x) : yellow

(+)
Tube 2(20x) : yellow (+

+)
Tube 3(30x) : yellow (+

+)
Tube 4(40x) : yellow (+
+)

fast.

Tube 5(50x) : yellow (+

++)
Blanco :
Starch solution 1% +
aquadest + iodine :

Blanko (B)
1 mL starch solution 1%
entered into test tube 1,2,3,4,5

yellow (+++)
After test using UVVIS:
- 10x : -0.062; A :
-

0.03
20x : -0.046; A :

added 0,5 mL diluted enzymes 10x 50x

0.014
30x : -0.037; A :

shaken and left until 1 minutes

0.005
40x: -0.036; A :

added 10 drops of iodine solution (with

temperature 60C out of steam bath)

0.004
50x : 0.038; A :

0.006
Blanco : -0.032

left until minimum 2 minutes

added 10 drops of aquadest

added 6 mL of aquadest
read the absorbant (A) with wavelength
680 nm
absorbant