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PROTEIN AND
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CARBOHYDRATE
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EXPERIMENT
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DEFRIZA LESWALDI | DIAN PERMATA |
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FITRA PARLINDO | NUR AIDIL FITRA
MUNAWWAR |NUR IKHLAS SYUHADA |
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RAHMAT SURYA | RIAN MUNANJAR |
YOGI RIZKY ANANDA
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Plus Senior High School
YEARS 2011 / 2011
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PROTEIN AND CARBOHYDRATE

EXPERIMENT
A. Tittle
Experiment for Protein and Carbohydrate
B. Aim

To determine the content of albumine

To determine the content of glucose, sucrose and amylum
solution

C. Theory

CONTENT OF ALBUMINE
Egg is one of proteins source of animal that very important for our
body. There are so many vitamins and nutrients from egg. Egg has
two part . They are Albumine and yolk. Egg consist of protein , A ,
D , E and B vitamins , fosfor and zinc. In egg , fat is lower. It is very
different with another proteins source of animal. One of adventage
from albumin is peptide substance. By peptide substance ,
spreading of disease in our body can be preventived. The work of
peptide substance is cast into prison for the bacteries , such as E.
Coli. Albumine also have no content of fat. In other side , Albumine
also can be function for beauty. Especially for treatment of face.
Using of Albumine as a masker can rejuvenating and brighting our
face.

PROTEIN EXPERIMENT

PROTEIN AND CARBOHYDRATE EXPERIMENT

To know contain of protein in albumine , we can do some experiment

. They are : Biuret experiment , Xantoproteat experiment and
Sulphur experiment.
Biuret Experiment Biuret experiment is general experiment for
protein (peptide bond), but can not show free of amino acid. Firstly,
give NaOH solution to albumine and then give CuS

O4

solution . If

colour become purple , it is mean that albumin consist of protein.

Xantoproteat

Experiment

Xantoproteat

experiment

is

experiment for protein that consist of Fenil cluster (Benzenes ring).

If protein consist of benzenes ring , then bring to the boil with thick
of Nitrit Acid , so will release yellow colour and then change to colors
ranging from orange to bright red, if made alkalis (base) with NaOH
solution.
Sulphur Experiment Sulphur experiment is experiment to know
consist of sulphur in protein. Firstly, give thick NaOH solution to the
protein solution then bring to the boil . Next , give some drop of Pb(C
H3

COO). If there is black sediment , it show that there is sulphur

in protein solution.

CARBOHYDRATE EXPERIMENT

PROTEIN AND CARBOHYDRATE EXPERIMENT

Carbohydrate is organic compound

that consist of carbon ,

hydrogen and oxygen. Function of carbohydrate for human as power

source and for plant as food back up form amylum, and also former
of framework for plant form selulose. Plant get glucose from
photosynthesis process.
Fehling Experiment

Carbohydrate like monosacaride and

disacaride but exception for sucrose can be identified by fehling

experiment. If positive consist of carbohydrate , so the solution will
release brick red of sediment. It caused by formed C

u2

O as a

result of that reaction .

Amylum

Experiment

Starch

or

amylum

are

Complex

Carbohydrate that can not solve in the water. Amylum is main

matter that released by plant to save so much glucose (as a product
of photosynthesis) in long time. Human and animal also using
amylum for power source that very important. Amylum consist of
two parts of carbohydrate . They are amylose and amylopectine , in
different composision. Amylosa give a hard characteristic while
amylopectine cause sticky characteristic. Amylosa give thick of
purple color on iodine test while amylopectine is not rection.

D. Apparatus and Materials

Apparatus

PROTEIN AND CARBOHYDRATE EXPERIMENT

Apparatus

Amount

o.
1.

Drop Pipette

As needed

2.

Measuring glass

As needed

3.

Clamper

As needed

4.

Shelf of Tube

1 pc

5.

Bunsen Burner

1 pc

6.

Fireplace Tripod

1 pc

7.

Mesh Wiring

1 pc

No.

Materials

Amount

1.

Egg

As needed

Materials

2.
3.

HN

O3

(solution)

NaOH (solution)

As needed
As needed

PROTEIN AND CARBOHYDRATE EXPERIMENT

4.
5.

Cu

SO 4

(solution)

Pb (CH 3 COO)

As needed
As needed

6.

(solution)
Glucose Solution

As needed

7.

Sucrose Solution

As needed

8.

Amylum Solution

As needed

9.

A Fehling Solution

As needed

10.

B Fehling Solution

As needed

11.

Iodin Solution

As needed

E. Procedure

CARBOHYDRATE EXPERIMENT
a) Fehling Experiment
Put 1 mL of glucose solution, sucrose solution and amylum

solution the test tube.

Give 5 drops of A Fehling.
Give 5 drops of B Fehling.
Shake the test tube.
Put it in the hot water.

b) Amylum Experiment
Put 1 mL of glucose, sucrose and amylum solution to the test
tube.
Give 3 drops of Iodin solution to each test tube.
Shake the test tube.

PROTEIN EXPERIMENT

PROTEIN AND CARBOHYDRATE EXPERIMENT

a) Biuret Experiment
Put 1 mL of albumine into test tube.
Give 5 drops of NaOH solution.
Then, give 5 drops of Cu SO 4 .
Shake the test tube.
b) Xantoproteat Experiment
Put 1 mL of albumine into test tube.
Give 5 drops of HN O 3 solution, then put it into hot water.
Then, give 20 drops of NaOH solution.
Shake the test tube.
c) Sulfur Experiment
Put 1 mL of albumine into test tube.
Give 10 drops of NaOH solution, then put it into hot water.
Give 10 drops of Pb (CH 3 COO) solution, then put it into hot
water.

F. Observation Result

Carbohydrate Experiment
a) Fehling Experiment
No.
1.
2.
3.

Solution
Glucose
Sucrose
Amylum

Fehling A
Blue
Blue
Blue

Fehling B
Blue
Blue
Blue

Solution
Glucose

Iodin
Not

Boiling
Red Brick
Blue
Blue

b) Amylum Experiment
No.
1.

PROTEIN AND CARBOHYDRATE EXPERIMENT

2.
3.

Sucrose

Change
Not

Amylum

Change
Purple

Protein Experiment
a) Biuret Experiment

No.

Object

NaOH

1.

Albumin

Colourless

Cu

SO 4

Purple

b) Xantoproteat Experiment

No.

Object

1.

Albumin

No.

HN

Object

O3

Boiling

NaOH

There is

There is

white

white

sediment

sediment

White

NaOH

Boiling

Pb

up side

Boiling

and yellow

(CH 3 COO) sediment

1.

Albumi

Colourl

White

White

ess

sedime

sediment

nt

down
side
There

is
sulfur
(black)

c) Sulfur Experiment

G. Conclution
From the experiment, we can conclude that :

PROTEIN AND CARBOHYDRATE EXPERIMENT

 Fehling experiment is testing for substance that consist of carbohydrate.

After boiling, the color of substance will change to be red brick. Why do
the substance can changing their color to be red brick ? Because, in
Fehling solution consist of

Cu 2 O .

Amylum experiment is testing for substance that consist of amylum.

After we shake test tube, if substance consist of amylum, so the color of
amylum solution will change to be purple.
Biuret experiment is testing for substance that there is peptide bonded.
If the substance consist of peptide bonded, the color will change to be
purple.
Xantoproteat experiment is testing for substance that consist of benzene
group. If the substance consist of benzene group, the colour will change
to be yellow or young red and also consist of sediment.
Sulfur experiment is testing for substance that consist of sulfur. If the
substance consist of sulfur, so there is sulfur on the white sediment of
albumin.

PROTEIN AND CARBOHYDRATE EXPERIMENT