Professional Documents
Culture Documents
1. Differentiate eukaryotic from prokaryotic cells, Eubacteria from Archaea, and know function of
endospores, capsules, and flagella.
a. Eukaryotic cells
i. Eukaryotic cells are typically much larger than prokaryotes.
ii. Eukaryotic cells have a membrane-bound nucleus and organelles, and a cytoskeleton
composed of microtubules, microfilaments, and intermediate filaments, which play an
important role in defining the cell's organization and shape.
iii. Eukaryotic DNA is divided into several linear bundles called chromosomes, which are
separated by a microtubular spindle during nuclear division.
iv. Cell division by separating duplicated chromosomes by mitosis or meiosis.
v. They can be unicellular or multicellular.
vi. Their flagella are flexible and move in a 2-D whip-like motion for motility.
b. Prokaryotic cells
i. Prokaryotic cells dont have a nucleus, but instead a nucleoid region, which lacks a
nuclear membrane.
ii. They generally lack membrane-bound cell compartments: such as mitochondria and
chloroplasts, and instead processes such as oxidative phosphorylation and photosynthesis
take place across the prokaryotic plasma membrane.
iii. They contain only a single loop of stable chromosomal DNA stored in the nucleoid.
iv. They reproduce through asexual reproduction, usually by binary fission or budding
v. Most are unicellular.
vi. Their flagella are rigid and rotate for motility
c. **Eubacteria
i. Their cell membranes are composed of fatty acid chains linked to glycerol in an ester
linkage.
ii. They contain a phospholipid bilayer
iii. They have peptidoglycan in their cell walls, composed of N-acetylglucosamine and Nacetylmuramic acid and have D-amino acids.
iv. Their flagella are hollow stalks, assembled by subunits moving up the central pore and
then adding onto the tip of the flagella.
d. **Archaea
i. Their cell membranes are composed of unique lipid isoprenoid branched chains linked to
glycerol in an ether linkage.
ii. In some the phospholipid bilayer is replaced by a single monolayer.
iii. Most lack peptidoglycan in their cell walls. Some instead have pseudopeptidoglycan,
composed of N-acetylglucosamine and N-acetyltalosaminuronic acid and dont have Damino acids.
iv. Their flagella are synthesized by adding subunits onto their base.
e. Endospores
i. A metabolically inactive, nonreplicating form of certain bacteria that is highly resistant to
physical and chemical damage.
ii. The primary function of most endospores is to ensure the survival of a bacterium through
periods of environmental stress.
iii. Reactivation of the endospore occurs when conditions are more favorable and involves
activation, germination, and outgrowth.
f. Capsules
i. A diffuse layer outside the cell wall of bacteria usually composed of polysaccharides.
ii. They help protect bacteria against phagocytosis.
iii. They contain water which protects bacteria against desiccation.
iv. They exclude bacterial viruses and most hydrophobic toxic materials such as detergents.
g. Flagella
i. Long, flexible, helical protein structures that extend from the surface of the cell and cause
motility by rotation.
ii. Filament: long, helical, hollow structure composed of the protein flagellin.
iii. Hook: short, curved structure like a universal joint connecting the filament and the
motor of the basal membrane in the cell.
iv. Basal body: composed of proteins that aggregate to form a rod to which four rings are
attached, acting as bushings to anchor the structure in the various layers of the cell
envelope.
2. ***Differentiate gram positive from gram negative microorganisms with regard to cell wall differences.
Also, describe cell wall functions.
i. the division of one bacterium into two identical, equal sized daughter cells
4. Describe structure of major macromolecules, nucleic acids, proteins, lipids, carbohydrates, their function
in living cells, the bonds linking their subunits, and their structure-function relationships.
a. Nucleic acids
i. Macromolecules such as DNA or RNA that carry genetic information and are composed
of chains of monomeric nucleotides connected by 3 5 phosphodiester linkages
ii. Nucleotides: phosphate, sugar, base
iii. Nucleosides: sugar, base
b. Proteins
i. Large organic compounds made of amino acids arranged in a linear chain joined together
by peptide bonds between the carboxyl and amino groups of adjacent amino acid residues
c. Lipids
i. Any fat-soluble (lipophilic), naturally-occurring molecule, such as fats, oils, waxes,
cholesterol, sterols, fat-soluble vitamins, or phospholipids
ii. The main biological functions of lipids include energy storage, acting as structural
components of cell membranes, and participating as important signaling molecules
iii. fatty acyls, glycerolipids, glycerophospholipids, sphingolipids, saccharolipids and
polyketides
d. Carbohydrates:
i. Simple organic compounds that are aldehydes or ketones with many hydroxyl groups
added, usually one on each carbon atom that is not part of the aldehyde or ketone
functional group
ii. Monosaccharides are the major source of fuel for metabolism, being used both as an
energy source and in biosynthesis.
iii. Polysaccharides are used for storage in the form is glycogen, especially in liver and
muscle cells. In plants, starch is used for the same purpose.
b. ***Adaptive immunity
i. Host defenses that are mediated by B and T cells following exposure to antigen and
exhibit specificity, diversity, memory, and self-nonself recognition.
c. Primary response
i. The lag of 5 to 7 days before antibody levels start to rise from the primary response is
time required for the activation of nave B cells by antigen and TH cells and for
subsequent proliferation and differentiation of the activated B cells into plasma cells.
ii. Antigen induces clonal proliferation of B lymphocytes into antibody-secreting plasma
cells and memory B cells.
d. Secondary response
i. The amplified population of memory cells accounts for the rapidity and intensity that
distinguishes the secondary response from the primary.
ii. The lag of 1 to 2 days occurs, antibody levels are higher, and they are sustained for much
longer
iii. The secondary response reflects the activity of the clonally expanded population of
memory B cells.
iv. ****Memory B cells respond to antigen more rapidly nave B cells and because there are
so many more memory B cells in the secondary response, more plasma cells are
generated, making antibody levels 100 to 1000 fold higher.
10. Describe the importance of self antigen expression for the immune system
a. Autoimmunity
i. The result from failure of the hosts humoral and cellular immune systems to distinguish
self from nonself, resulting on an attack on self cells and organs by auto-antibodies and
self-reactive T cells.
b. Self antigen
i. A small molecule, usually a peptide of 68 amino acids derived from body proteins; that
is, "self" proteins such as proteins within the cytosol or serum proteins; i.e., proteins
circulating in the blood and lymph.
ii. Self antigen is nestled in a histocompatibility molecule thats encoded by the MHC, class
II for CD4+ T cells and class I for CD8+ T cells.
c. Central tolerance
i. Deletes T- or B-cell clones before the cells mature if they possess receptors that recognize
self antigens with greater than a low threshold affinity.
ii. T cells whose receptors bind these epitopes so tightly that they could attack the cell
displaying them are deleted by apoptosis.
iii. T cells that survive this negative selection leave the thymus and migrate throughout the
immune system.
11. Describe the structure and functions of immunoglobulins, including antigen binding sites and roles in
immunity
a. ***Ig Structure
i. Two identical light (L) chains are bound to two identical heavy (H) chains by disulfide
bonds and noncovalent interactions to form a heterodimer.
ii. Similar noncovalent interactions and disulfide bridges link the two identical H-L chain
combinations to each other to form the basic four-chain (H-L)2 antibody structure, a dimer
of dimers.
1. Variable regions
a. The first 110 or so amino acids of the amino terminal of heavy and light
chains vary greatly among antibodies of different antigen specificity.
2. Constant regions
a. The nearly invariant portion of the Ig
b. The amino acid sequence of the constant region determines the isotype (,
, , , and ) and the type ( and ) of the light chains.
3. Fab fragment
a. A monovalent antigen-binding fragment of an Ig molecule that contains
one light chain and part of one heavy chain, linked by a disulfide bond.
4. Fc fragment
a. A non-antigen-binding fragment of an Ig molecule that contains the
carboxyl-terminal portion of both heavy chains.
a.
b.
c.
d.
e.
f.
2.
3.
4.
5.
6.
multilobed nucleus
granulated cytoplasm that stains with both acid and basic dyes
first to arrive at a site of inflammation
phagocytic
migrate to tissues where they live 1-2 days
increase in number of circulating neutrophiles bc of inflammation =
leukocytosis
g. movement of circulating neutrophils into tissues = extravasation
Eosinophils
a. bilobed nucleus
b. granulated cytoplasm that stains with acid dye eosin red
c. play a role in defense against parasitic organisms by secreting contents of
granules
d. phagocytic
Basophils
a. lobed nucleus
b. heavily granulated cytoplasm that stains with basic dye Methylene blue
c. release pharmacologicallyactive substances from granules that have a
major role in allergic responses
d. nonphagocytic
Mast cells
a. Precursors formed in bone marrow and are released into the blood as
undifferentiate cells; do not differentiate until they leave the blood and
enter the tissues
b. have granules that contain histamine and other pharmacologically active
substances
c. role in allergy development
Dendritic cells
a. Main function: antigen presentation to T cells
b. All express class I and II MHC
c. Acquire antigen
i. Phagocytosis
ii. Receptor-mediated endocytosis
iii. Pinocytosis
Follicular dendritic cells
a. Do not express class II MHC
b. Dont function as APCs
c. Do not arise in the bone marrow
d. Found in lymph node follicles
e. Participate in B cell maturation
ii. Transcription
1. occurs in the cytoplasm alongside translation
2. mRNA is not modified
iii. Elongation
1. It starts with the "abortive initiation cycle," during which RNA Polymerase will
synthesize mRNA fragments 2-12 nucleotides long.
2. This continues to occur until the factor rearranges, which results in the
transcription elongation complex
iv. ***Termination
1. Rho-independent
a. RNA transcription stops when the newly synthesized RNA molecule forms
a G-C rich hairpin loop, followed by a run of U's, which makes it detach
from the DNA template.
2. Rho-dependent
a. A protein factor called "Rho" destabilizes the interaction between the
template and the mRNA, thus releasing the newly synthesized mRNA
from the elongation complex.
v. ***Regulation
1. Repressors bind to regions called operators that are generally located downstream
from and near the promoter.
2. Activators bind to the upstream portion of the promoter, such as the CAP region.
3. A combination of activators, repressors and rarely enhancers (in prokaryotes)
determines whether a gene is transcribed
b. Eukaryotes
i. Replication
1. ***Origin = multiple ori
2. ***Telomeres- why do they have telomeres (because theyre linear, because
theres more than one??)
3. linear chromosome
4. Nucleosomes
ii. Transcription
1. transcription primarily occurs in the nucleus; translation occurs in the cytoplasm
2. DNA is wound around histones to form nucleosomes and packaged as chromatin
3. eukaryotic mRNA is modified through RNA splicing, 5 capping and poly A tail
addition
4. **RNA polymerase does not directly recognize the core promoter sequence
5. **Only after certain transcription factors are attached to the promoter
(transcription initiation complex) does the RNA polymerase bind to it
iii. Elongation
1. In Eukaryotic transcription the polymerase can experience pauses, which may be
intrinsic to the RNA polymerase or due to chromatin structure.
2. Often the polymerase pauses to allow appropriate RNA editing factors to bind.
iv. Termination
1. Transcription termination in eukaryotes is less well understood.
2. It involves cleavage of the new transcript, followed by template-independent
addition of As at its new 3' end, in a process called polyadenylation.
v. Regulation
what does the bacterial cell wall do (help in time of drought, help invade hosts, helps in phagocytosis)?
The peptidoglycan layer allows the cell to retain cell structure even with osmotic pressure. During times of a
drought the cell retains all water within the cell. The peptidoglycan allows for resistance of lysozyme digestion.
Gram positive, having 95% peptidoglycan, are very resistant to lysozyme. How does it invade its host?