Professional Documents
Culture Documents
Service Manual
Table of Contents
Page
1.
2.
2.1
2.2
2.3
Safety Instructions
Shipping Crate
Unpacking the Unit
3.
Pump setup
10
4.
11
4.1
4.2
4.3
4.4
5.
Front View
Rear View
Rear View with Component panel removed
Top View
16
System Configuration
5.1
Loading of system F/W
5.2
Loading of system S/W
5.3
Serial Command Interface
5.3.1
5.3.2
Serial configurations
Command Set
Table of Contents
Page
6.
6.1
6.5
7.
7.1
10.
36
31
9.
8.
22
Dispensing Assembly
40
53
Table of Contents
Page
11.
Tests
11.1
11.2
11.3
12.
59
Performance Test
Leak Test
%CV Test
Electrical Information
62
13.
Troubleshooting
72
14.
Warranty
73
15.
74
16.
Forms
75
Installation Report
No.
4
1
5
2
9
8
10
11
Description
pipetting needles
labware platform
labware stations
power indicator
actuator
ON/OFF switch
10
RS232C/422 connector
11
actuator motor
The strapping will rebound downward away from you. This will help prevent
personal injury. Other people should remain at a safe distance.
The strapping will rebound to the right away from you. This will help prevent personal
injury. Other people should remain at a safe distance.
SEE
SEE
SEE
Stage
Labware
Platform
Platform
Machine is ready
for re-location.
Lift the mac hine base
when moving.
SEE
Lift here
only!
Machine
base
2
2.3 Unpacking the Unit
Care should be exercised when unpacking the shipping crate. Please
follow these steps:
1.
Lift the unit up by placing your hands at the spots indicated by the two
arrows. Keep your back straight to avoid possible injury (we
recommend this task to be handled by two people together as the
unit is quite heavy).
3. Pump Setup
Pipet Tip Wash Bath & Pump
Setup Illustrations
(For two pump head system)
Tubing 17
Channel
Pipet
tip Wash Bath
Tubing Adaptor
#17
#17
#17
Tubing 17
Pum p
Tubing 17
Tubing 17
Clean
Fresh
wash
water
fluid
Waste Container
#18
#17
#17
Page 1
Version 1. 0
09/ 2004
IN
OUT
PUMP
COMPUTER
10
Page 2
11
Power-On connector
Dispensing Assembly
Linear
Z ZLinear
Bearing
Y-Motor
P/N 961-550-EP-24
Labware Platform
12
Power Supply
P/N 961-EP-201
Ventilation Fan
P/N 961-550-EP-80
Power Supply
P/N 961-EP-200
Stepper Motor
Driver
P/N 961-550-EP-34
13
Z-Motor
P/N 961-EP-204
X-Motor
P/N 961-EP-205
14
Y-Motor
P/N 961-550-EP-24
CPU Board
961-EP-59
15
16
F/W Chart
File name
Revision level
1-20+
Machine Type
6PC961XL-01
18
19
This command will initializes the unit once, and all motor always run in slow
speed, sequence is: synQUAD motor, Z motor, plunger motor, X motor and Y motor, and
before initializing synQUAD motor, a pulse signal will send out to synQUAD unit, and
after INIT command, all parameters will restore to default value.
Mode mode_val:
This command sets the operating motor speed exclude Plunger motor, MODE 0
is the higher speed, and MODE 2 is the slowest speed. A MODE command must always
follow INIT command to define motor speed.
NMVY = move_Y_axis_value:
Moves Y-axis (Shuttle) when synQUAD scan plate. Machine will generate a
starting signal to synQUAD unit after a period of acceleration of moving. The command
works with NMYS and SSYM in more situations.
Example:
NMYS 50
SSYM 95
NMVY = 3000
NMYS speed_val:
Set NMVY moving speed that speed_val is range 50 to 150. Smaller value moves
at faster speed.
Example:
NMYS 75
SSYM starting_pos_val:
Define generating starting signal position. Starting_pos_val is range 1 to 206,
larger value starts to scan earlier.
Example:
SSYM 204
SSFC:
20
SYNQ 0:
Set synQUAD carriage to home, the command will force carriage back to top
position until hits home sensor.
PGUP asp_step:
Aspirate command. This causes the plunger to move up an amount steps. The
value must not be made total up-moving step larger than the maximum value.
Maximum step is 48107(may change in small range).
Example:
PGUP 20000
PGDN disp_step:
Dispense command. This causes the plunger to move down an amount steps. The
value must not be made total down-moving step lease than 0.
Eaxmple:
PGDN 20000
PGEM:
Dispense command. This causes plunger to move down to the lowest position that
plunger will be empty.
PLGS plunger_speed:
Set Plunger moving speed. The value is range 0 to100. Larger value moves at
slower speed.
Example:
PLGS 50
STAT:
Return status information, such as the current position of all motor, machines
parameter, and Firmware version, etc.
ELVX dir disp
ELVY dir disp
ELVZ dir disp
Moves the specified elevator/tray in the direction specified for the number of step
clocks specified. Dir is either + or -. The coordinate system used is a left handed one when
facing the unit. Z is up, y is to the users right, x is toward the user.
Example:
ELVX = value
ELVX value
ELVX + value
BLOW
This command forces the plunger to rapidly move down below the 0 volume
position and blow out any volume remaining in the tips left over from the EMPT
operation. After a small delay the plunger is moved back up to the 0 volume position. This
command is invalid except when the plunger is at the 0 volume position, due to the EMPT
command or a series of DISP commands.
21
6. Dispensing Assembly
6.1 Dispensing Assembly Removal
6.2 Plunger Limit Switches Adjustment
22
Tools Needed:
Philip Hd screw Driver
Hex Key for #8 SHCS
Hex Key for #4 SHCS
HDX Key for #10 SHCS
Mini Ratchet wrench w/ Hex Bit for # 6 SHCS
23
Step 1.
1.1 Remove 4x #4 SHCS
1.2 Remove Unit A
Unit A
24
Step 2.
2.1 Loosen 2x #8 SHCS
Plunger Motor
Lead Screw
Plunger Cover
25
Step 3.
3.1 Remove 4x #6 S HCS for
the Plunger Plate
Puller and the Plunger
Cover
Plunger Puller
Clamp
Plunger Cover
26
Stabilizer
Step 5.
5.1 Remove 2x #10 SHCS
that connect the
entire Dispensing
Assembly to the
Vertical Plate
5.2 Use both hands, one on
each side of the
Dispensing
Assembly, pull it
straight toward you
5.3 Removal of the
Dispensing
Assembly complete
27
Upper Bracket
Plunger Plate
Puller
Lower Bracket
Position A
Tools Needed:
Hex Key for #4 Socket Cap Screw
Notes:
1.
For first time installation, Lower Limit Switch location
must be set before Upper Limit Switch Location.
2.
Setup serial command interface using HyperTerminal (see section 5.3)
3.
Initialize Pipettor.
28
~1mm
6.5.1
6.5.1.1
6.5.1.2
Check the distance between Plunger Tip and the Teflon plug
to see if reading is ~1mm. If not, continue with the following
procedure.
6.5.1.3
6.5.1.4
6.5.1.5
Note:
6.5.1.6
6.5.2
6.5.2.1
6.5.2.2
29
6.5.2.3
6.5.2.4
6.5.2.5
6.5.2.6
30
31
Tools Needed:
7.1.2
7.1.3
7.1.4
7.1.5
32
Locating Pin 2
X-Position Sw
Set Screw
For x position Adjustment
Tools Needed:
Hex Key for #4 Socket Head Set Screw
Notes:
1. Execute Hyper Terminal
2. Initialize Pipettor.
33
Y-position Switch
7.2.1
7.2.2
If microtiter plates are either too tight of too loose within the
Locating Pins, adjust the Offset Locating Pins to achieve fitness ( 2
per position ).
7.2.3
Raise the Stage so that tips are closed to the surface of the wells
by sending command elvz_=_5000
7.2.4
Send command elvy_=_215 and the tips should align with the
centers of the microtiter plate. (in Y direction)
7.2.5
Y - Direction Alignment
7.2.6
7.2.7
7.2.8
7.2.9
7.2.10
34
Figure 7A
X Direction Alignment
1
12
12
X - Direction Alignment
7.2.11
7.2.12
7.2.13
7.2.14
7.2.15
7.2.16
7.2.17
7.2.18
7.2.19
35
36
Z- Motor Shaft
X-Motor Shaft
Y-Motor Shaft
Supplies Needed:
Lithium Grease, WD 40
Notes :
8.1.1
8.1.2
8.1.3
8.1.4
38
4x #8 Screw
1
#8 Set Screw
2
#8 Set Screw
3
X-Motor Shaft
4
Stage
1
39
40
2x #8 Screw
14
Stabilizer
2x #10 Screw
18
Plunger
Motor Shaft
16
Plunger Motor
Shaft Clamp
15
Plunger
Motor Shaft
19
2x #8 Screw
14
Wire Tie
3
Stage Spring
1
Z-Motor Connector
2
X-Motor Connector
2
X-Motor Outer
Set Screw
6
X-Motor Inner
Set Screw
5
X-Shaft
12
3x #10 Screw
18
Z-Motor Shaft
Clamp
20
2x #8 Screw
20
Vertical Plate
Assembly
19
Motor Bracket
Assembly
21
41
9.1.2
9.1.3
9.1.4
9.1.5
9.1.6
9.1.7
Remove the outer set screws (2x #8 SHSS) for the X-Shaft
End Support
9.1.8
Loosen the inner set screws (2x #8 SHSS) for the X-Shaft
End Support
9.1.9
9.1.10
9.1.11
9.1.12
9.1.13
9.1.14
9.1.15
42
9.1.16
Hand turn the Plunger Motor Shaft clockwise until it clears the
Plunger Plate Puller
9.1.17
9.1.18
Remove 5x #10 SHCSs that connect the Top Plate and the
Base.
9.1.19
9.1.20
9.1.21
9.1.22
9.1.23
43
Z-Motor
P/N:550-EP-25
Motor
Bracket
X-Motor
P/N:550-EP-23
9.2.2
Note:
Make sure that the wires are to the left of the motor from the
drawing above
9.2.3
Z-Motor
P/N:550-EP-25
Motor
Bracket
X-Motor
P/N:550-EP-23
9.3.2
Note:
Make sure that the wires are to the right of the motor from the
drawing above
9.3.3
Z-motor
Connector
13
Stage
3
X-Motor
Connector
1
X-Motor Outer
Set Screw
4
X-Motor Inner
Set Screw
5
X-Shaft
6
4 x #10
SHCS
16
Adaptor Plate
4 x #8 SHCS
7
Adaptor Plate
2 x Dowel Pin
8
X-Motor
Adapter Plate
9
46
X-Motor
9.4.1
9.4.2
9.4.3
9.4.4
9.4.5
9.4.6
9.4.7
9.4.8
9.4.9
9.4.10
9.4.11
9.4.12
Note: Make sure that the front-end of X-motor shaft seats securely inside the
locking plate before tighten up the setscrew during X-Motor re-installation.
Z-Motor
9.4.13
9.4.14
9.4.15
9.4.16
9.4.17
9.4.18
9.4.19
Note: Hand turn Z-Motor Shaft counter-clockwise until its front end touches the
up-most part of the Shaft Locking Block during Z-motor re-installation.
47
9.5
Labware
Level Adjustment
Labware Platform
Level Stage
Adjustment
No.
4
1
Tools required
48
Description
Labware Platform
Labware
station
Labware
stage
Labware station
Actuator assembly
Elevator
Base plate
CAUTION:
Tu rn power o ff
Lower Lab ware platform
as shown by arrows
SEE
Linear Bearings
Centering the Labware station
SEE
SEE
SEE
49
SEE
Linear Bearings
Slightly loosen 8 screws
fro m the Linear bearings
SEE
SEE
50
SEE
Tighten 4 screws
SEE
Loosen
upper 4
screws
fro m
Linear
Bearings
tighten
the upper 4 screws with #3 met ric
L-Hex Key
(4 screws fro m Linear Bearings)
YES
SEE
Gap between
Elevator and
Base plate?
NO
Tighten 4 screws
SEE
51
Place
platform
Place Labware
Lab ware stage
onto
actuator
assembly
onto actuator assembly
SEE
Place
Labware
platform
onto
actuator
Place
Lab
ware platform
onto
actuator
Until
until
4 screws
of Labware
platform
align
all All
4 screws
of Labware
stage
align with
thewith
4
the
4
holes
of
actuator
assembly
(see
above)
holes of actuator assembly(see above)
Tighten 44 screws
m the
Tighten
screwsfrofrom
Labware
stage
the Labware platform
SEE
Tighten
4 screws
with
9/ 64
Hex
key
Tighten
4 screws
with
9/64
Hex
key
((Locate
locate atatthe
center
of
Labware
stage)
the center of Labware platform)
DONE
52
Teflon
Tip
Holder
Teflon
Anchor
Hand-Help
Tip Fixture
(HHTF)
Spacers
Long Nose Pliers
53
Spacer installation
The spacer will provide even level support during tip
removal and installation. Spacers are to be placed
between the assembly unit and the supporting plate.
Locate the bad tip, then slide spacer on the left and
to the right of bad tip. See diagram for example.
Tip Removal
Remove the bad tip with Long Nose Pliers
Figure 1
Position 1
Position 3
Figure 3a
Figure 3b
Figure 4a
Figure 3c
Figure 4b
54
Figure 5a
5.1
5.2
Figure 5b
NOTE:
You are now ready to test the length of an existing
tip, this test will help you to plant a new tip into the
assembly during the new tip installation procedure.
However getting the correct measurement is a
delicate procedure that must be done precisely.
Failure to do so will result in another tip removal.
Existing Syringe
Tip
STOP turning
the knob of
Measurement
Unit when it
touch Syringe
tip end.
Tip
Measurement
Knob
Figure 5c.
Tip Measurement Device Diagram
5.3
NOTE:
You must STOP turning immediately when it touch
the tip end, overturning the screw will push the
existing tip out of position, which will result in
another tip removal. See diagram for further
explanation. Tip measurement procedure is done.
Figure 5d
55
6.1
6.2
Insert the shinning end of new tip into the Teflon Tip
Holder,
6.3
Syringe Tip
Coated Surface
Figure 6b
Figure 6c
Figure 6d
56
7.1
7.2
7.3
Push the tip into the assembly until the top part of
Tip Measurement Device reach the bottom of HHTF
completely. Tip installation procedure completed.
7.4
Syringe Tip
Closing of Tip
Measurement
Device
Figure 7b
Figure 7c
Tip
Measurement
Knob
Figure 7d
57
HHTF Removal
Remove the HHTF vertically such that it does not
blend tips during the HHTF removal procedure.
Figure 8
Figure 9
10
Spacer Removal
Remove spacers
Figure 10
Installation Complete
58
11. Tests
11.1
Performance Test
6. Check all the plates on the corresponding stations and change to the
synQUAD instead of TIP. Fill some water into 96 well plate and tube. Make
sure the end of one soft pipe from synPump is inside water.
7. Run the entire protocol and check for abnormal operation.
59
3. Run the protocol. When the operation is pause, check for even droplet
at tips.
4. Raise reservoir manually to remove droplets.
5. Check for sign of leakage after 2 minutes
6. Click Continue to finish empty function.
60
11.3
%CV Test
3
4
5
6
7
Fill the CV test liquor into the shallow reservoir on station 4 and fill water into the
deep reservoir on station 1.
Run protocol line 1 and line 3 only. Do aspirate and empty for 4 cycles to
simulate pre- mix.
Run protocol line 1, line 2 and line 3 only to make %CV test sample plate.
If need more sample plates, continue to dispense on station 2 then empty, or run
the three lines more times. Then take the sample plates to do %CV test.
After the needed sample plates are done, run protocol line 4 only to wash 96
tips, then change to clean water to repeat wash for several times (increase the
wash volume by 1ul for each time) until there is no test liquor color in the water.
It better to use alcohol to wash 96 tips or soak the tips into alcohol for several
hours if dont use pipettor for long time after the %CV test. Fill clean water to wash
tips for two times before use the pipettor.
61
62
63
64
65
66
67
68
69
70
W15
71
13. Troubleshooting
1.
Problem:
Leakage
Signs:
Solution:
2.
Problem:
Solution:
3.
Problem:
Solution:
72
14. Warranty
One Year Limited Warranty
Apricot Designs Inc. warrants to the original purchaser of the 96 Channel Syringe
Based Pipettor that the instrument will meet the original manufacturers
performance specifications, and that the instrument will be free from defects in
material and/or workmanship in normal operation for one year from the date of
delivery.
If the instrument is found to be defective during the warranty period, Apricot
Designs Inc. will repair or replace any defects in material and/or workmanship, or
any failure of the system to conform to specifications, at no charge. The original
purchaser must notify Apricot Designs Inc. of any defects, in writing, before the
expiration date.
This warranty covers normal use and does not apply to the following:
y
73
Description
961-A-01
2
3
961-A-03
961-P-01C-100-100
961-P-02C-100,
5
6
961-P-03C-100,
961-P-01C-RN-NC-100
7
8
9
10
11
12
13
14
15
16
17
18
19
20
21
22
23
961-EP-58
961-EP-59
961-EP-200
961-EP-201
961-550-EP-35
961-550-EP-34
961-EP-202
961-EP-203
961-550-EP-21
961-EP-204
961-550-EP-24
961-EP-205
961-550-EP-01
961-550-EP-07
961-550-EP-71
961-550-EP-75
961-550-EP-80
74
16. Forms
Installation Report
75
2)
3)
b.
To the Instrument
c.
____________________________________
4)
____________Hz
5)
6)
Instrument Initialization:
Plunger Home _______________________________________________
X-Home _________
7)
Y-Home _________
Z-Home _________
Basic Operations:
a.
c.
d.
e.
8)
9)
10)
Accessories Demonstration:
Tip Loader _____________________ Tip Ejector __________________
Pipetting Head Lubrication Box _________________________________
11)
Institute __________________________________________________
Contact __________________________________________________
Address __________________________________________________
Telephone Number _________________________________________
E-Mail ____________________________________________________
Installed By ________________________________________________
Date _____________________________________________________
Details of difficulties encountered:
__________________________________________________________
__________________________________________________________
__________________________________________________________
__________________________________________________________
__________________________________________________________
__________________________________________________________
__________________________________________________________
__________________________________________________________
77
78