1KPh

Group No. 7
Sampang, Senseng, Soriano E., Soriano M., Taytay, To

Anaerobic Respiration: Alcoholic Fermentation

I. Introduction
The single-celled fungus Saccharomyces
cerivisiae, a yeast, is capable of both aerobic and
anaerobic respiration. When oxygen is present, it
breaks down glucose aerobically following the
metabolic sequence of glycolysis, intermediate
reactions, Krebs cycle, and electron transport
system. Without oxygen, only glycolysis takes
place. Glucose is metabolized to pyruvate which, in
turn, is converted to two waste products: carbon
dioxide and ethanol.
II. Objectives
To show both aerobic and anaerobic
respiration by yeast cells
To demonstrate the presence of the end
products carbon dioxide and ethanol
To observe the rate of anaerobic respiration
among given samples with various time
conditions
III. Materials
3 fermentation tubes
Cotton
50 mL 10% glucose
30 mL 2% peptone
0.5%
yeast
extract
(Saccharomyces
cerivisiae)
Live Yeast (Saccharomyces cerivisiae)
5 Test tubes and a test tube rack
Phenol Red solution
1 mL I2KI
1.5 mL 1.5 M NaOH
IV. Methodology
Fill the 3 fermentation tubes up to the arm with
nutrient medium. (Glucose, peptone, and yeast
extract)
Plug the mouth of
the
fermentation
tube with cotton
and sterilize.

Describe
the
appearance of the
medium
in
the
fermentation tube A.

Add live yeast suspension to Fermentation tube

B. Observe the result after 2 days.
Add live yeast suspension to Tube C. Observe
the results after 5 days. Remove the cotton plug
and smell the content.
To prove the presence of CO2:
Transfer to tube 1 a pipettefull of fermentation
medium from tube C. To tube 2, place a similar
pipetteful of distilled water.

Add to both tubes one drop of phenol red

solution. Red in alkaline (basic); yellow in acidic
Iodoform test: To prove the presence of
ethanol
Tube 1: 2.5 mL distilled water
Tube 2: 1.25 mL distilled water and 1.25% ethanol
Tube 3: 2.5 mL medium from fermentation tube C
Add to each
tube 1 mL
strong I2KI and
1.5 mL 1.5M
NaOH.

Mix the content and allow to

stand for 5-10 minutes. If
ethanol is present, yellow
precipitate will settle.

Examine the remaining yeast from the fermentation

tube C under LPO and HPO of a microscope.
V. Results
Fermentation tube b was
observed after 2 days. It showed
some bubbles which proved the
presence of CO2. It also smelled
pungent.
While fermentation tube c
was observed after 5 days. It has
no bubbles but smelled like alcohol.
The iodoform test and the
test using phenol red solution
proved that fermentation tube c
has really undergone anaerobic
respiration. It showed the product
of anaerobic respiration which is ethanol and
carbon dioxide.
VI. Conclusion
It is concluded that the rate of anaerobic
respiration among yeast cells varies due to the
internal and external conditions. As glucose and
fructose breaks down to generate energy due to
lack of oxygen, ethanol and carbon dioxide forms
as a side effect. The real difference between
aerobic and anaerobic respiration is that with
oxygen, more energy is produced. Without oxygen,
more alcohol is produced.
VII. References
http://www.woodlandbrew.com/2013/03/yeastpropogation-with-aerobic.html?m=1
Vasquez, Laurente, & Cobar. (2013). Laboratory
Manual for Pharmaceutical Botany.