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Based on the information, design an experiment to be conducted in the laboratory to investigate the
effect of the number of leaves on the rate of transpiration in a hibiscus plant.
Berdasarkan maklumat ini, rancang satu eksperimen untuk dilaksanakan di dalam makmal untuk
mengkaji kesan bilangan daun ke atas kadar transpirasi satu pokok bunga raya.
The planning of your experiment must include the following aspects:
Perancangan eksperimen anda hendaklah meliputi aspek-aspek berikut:
Problem statement
Pernyataan masalah
Objective of investigation
Objektif kajian
Hypothesis
Hipotesis
Variables
Pemboleh ubah
List of apparatus and materials
Senarai radas dan bahan
Technique used
Teknik yang digunakan
Experimental procedures
Kaedah eksperimen
Presentation of data
Persembahan data
Conclusion
Kesimpulan
1
[17 marks]
[17 markah]
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Suggested Answer:
Problem
Statement
1. Does the number of leaves affect the rate of transpiration (in hibiscus plants)?
2. What is the relationship between the number of leaves and the rate of transpiration
(in a hibiscus plant)?
Aim
To investigate / determine the relationship between the number of leaves and the
rate of transpiration in a hibiscus plant.
Hypothesis 1. The more the number of leaves, the higher the rate of transpiration.
2. When the number of leaves increases, the rate of transpiration increases.
Variables
Manipulated variable: Number of leaves / stomata
Responding variable: Distance travelled by air bubble (in five minutes) // the rate of
transpiration
Controlled variable: Type of (terrestrial) plant / hibiscus // light intensity //
surrounding temperature
Materials
Materials: *Hibiscus shoot / plant, water and plasticine
and
Apparatus: *Ruler / weighing balance, capillary tube + rubber tubing // potometer //
Apparatus
stoppered conical flask, beaker / basin, (sharp) knife, stopwatch, string /
marker pen and tissue paper / filter paper
Technique Recording the distance travelled by air bubble in five minutes using a stopwatch.
Calculating (and record) the rate of transpiration by the formula:
Rate of transpiration =
Procedure
Example of
Procedure
P1
P1
P5
P5
P1
P1
P5
P1
P5
P5
P1
P1
P1
P1
P5
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Sample answer:
Distance travelled by air bubble in 5 minutes
Number
(cm)
of leaves
First reading Second reading
Average
P4
P4
P4
P2
P3
P4
Rate of
transpiration
(cm minute 1 )
or
Number
of leaves
Conclusion
Planning
the
Experiment
Rate of
transpiration
(cm minute 1 )
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Suggested Answer:
Problem
What is the relationship between total surface area per volume ratio and the rate of
Statement diffusion?
Hypothesis The larger the total surface area per volume ratio / the smaller the size of cube, the
faster the rate of diffusion.
Variables
Manipulated variable: Total surface area per volume ratio / the size of cubes
Responding variable: Rate of diffusion
Fixed variable: Coloured water temperature / time taken
Materials
Materials: Potato and coloured water
and
Apparatus: Penknife, white tiles, beaker, forceps, basin and girded transparency
Apparatus
Example of 1. Potatoes are cut into 0.5cm, 2cm, 3cm and 4cm length of sides cubes each.
Procedure 2. Then the cubes are immersed into a basin containing 100ml of coloured water.
3. All the cubes are left for 30 minutes.
4. After 20 minutes, the outer surface of the cubes are dried with filter paper and cut
into two halves.
5. The percentage of coloured area in each cube is estimated by using a girded
transparency.
6. The rate of diffusion of each cube is calculated by using a formula:
Rate of diffusion
Data
Percentage
of coloured
area (%)
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Table 1 / Jadual 1
(a) Record the volume of urine collected in the boxes provided in Table 1.
Rekod isipadu air kencing yang dikumpul pada kotak yang disediakan dalam Jadual 1.
[3 marks]
(b) (i) State two different observations made from Table 1.
Nyatakan dua pemerhatian yang berbeza yang dibuat daripada Jadual 1.
Observation 1 / Pemerhatian 1:
_______________________________________________________________________
Observation 2 / Pemerhatian 2:
_______________________________________________________________________
[3 marks]
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______________________________
Responding variable
Pemboleh ubah bergerak balas
______________________________
______________________________
Constant variable
Pemboleh ubah dimalarkan
______________________________
______________________________
Table 2 / Jadual 2
[3 marks]
(d) State the hypothesis for this experiment.
Nyatakan hipotesis bagi eksperimen ini.
_________________________________________________________________________
[3 marks]
(e) (i) Construct a table and record all the data collected in this experiment.
Bina satu jadual dan rekodkan semua data yang dikumpul dalam eksperimen ini.
Your table should have the following aspects:
Jadual anda hendaklah mengandungi aspek-aspek berikut:
Concentration of drinking water
Kepekatan air minuman
Volume of drinking water intake
Isi padu air minuman yang diambil
Volume of urine collected
Isi padu air kencing yang dikumpul
Percentage of water reabsorbed by kidney
Peratus air yang diserap semula oleh ginjal
Use the formula:
Percentages of water reabsorb by kidney
Volume of drinking water intake - volume of urine collected
=
Volume of drinking water intake
7
100%
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Gunakan formula:
Peratus air yang diserap semula oleh ginjal
Isi padu air minuman yang diambil - isi padu air kencing yang dikumpul
=
Isi padu air minuman yang diambil
100%
(f) Based on the graph in 1(e)(ii), explain the relationship between the percentage of water
reabsorbed and the concentration of drinking water intake.
Berdasarkan graf di 1(e)(ii), terangkan hubungan antara peratusan penyerapan semula air dengan
kepekatan air minuman yang diambil.
__________________________________________________________________________
[3 marks]
(g) State the operational definition for osmoregulation.
Nyatakan definisi secara operasi bagi pengosmokawalaturan.
__________________________________________________________________________
[3 marks]
(h) After three hours, the student was given 500ml of coffee. Predict the volume of his urine output.
Explain your answer.
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Tiga jam kemudian, pelajar tersebut diberi minum sebanyak 500ml air kopi. Ramalkan isi padu
air kencing yang dihasilkan. Terangkan jawapan anda.
__________________________________________________________________________
[3 marks]
(i) The following lists are part of apparatus and materials used in this experiment.
Senarai berikut adalah sebahagian daripada radas dan bahan yang digunakan dalam eksperimen
ini.
Drinking cup, stopwatch, urine collected, measuring cylinder, sodium chloride
solution, student
Cawan minuman, jam randik, air kencing yang dikumpul, silinder penyukat,
larutan natrium klorida, murid
Complete Table 3 by filling each variable with the apparatus and materials used in this experiment
in the space provided.
Lengkapkan Jadual 3 dengan mengisi setiap pemboleh ubah dengan bahan dan radas yang
digunakan dalam eksperimen ini di dalam ruang yang disediakan.
Variables
Pemboleh ubah
Manipulated variables
Pemboleh uhah dimanipulasi
Responding variables
Pemboleh ubah bergerak balas
Controlled variables
Pemboleh ubah dimalarkan
Apparatus
Radas
Materials
Bahan
Suggested Answer:
(a)
Concentration of drinking water
Kepekatan air minuman
(%)
0.0
5.0
62
10.0
46
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(ii)
The volume of urine collected after taking 10.0% concentration of drinking waler is 46ml.
Isi padu air kencing yang dikumpul selepas minum air dengan kepekatan 10.0% ialah 46ml.
Inference for observation / Inferens untuk pemerhatian 1:
At the concentration of drinking water is 0.0% distilled water, the volume of urine collected is
the highest, because osmotic pressure decreases, therefore the secretion of ADH //
permeability of kidney tubule towards water // volume of water reabsorb decreases.
Pada kepekatan air minuman 0.0% air suling, isi padu air kencing yang dikumpulkan ialah
paling tinggi, kerana tekanan osmotic rendah, rembesan ADH // ketelapan tubul ginjal
terhadap air // isi padu air yang diserap semula menurun.
Or / Atau
As the concentration of drinking water increases, the volume of urine collected is decreased,
because osmotic pressure increases, therefore the secretion of ADH // permeability of kidney
tubule towards water // volume of water reabsord increases.
Semakin meningkat kepekatan air minuman, isi padu air kencing yang dikumpul menurun,
kerana tekanan osmotic meningkat, rembesan ADH // ketelapan tubul ginjal terhadap air // isi
padu yang diserap semula meningkat.
Inference for observation / Inferens untuk pemerhatian 2:
At the concentration of drinking water is 5.0% / 10.0%, the volume of urine collected is lower /
the lowest, because osmotic pressure increases, therefore the secretion of ADH //
permeability of kidney tubule towards water // volume of water reabsorb increases.
Pada kepekatan air minuman 5.0% / 10.0%, isi padu air kencing yang dikumpulkan ialah
rendah / paling rendah, kerana tekanan osmotic menjadi tinggi, rembesan ADH // ketelapan
tubul ginjal terhadap air // isi padu air yang diserap semula meningkat.
(c)
Variable
Pemboleh ubah
Concentration of drinking water
/ Concentration of sodium
chloride solution (NaCl)
Kepekatan air minuman /
kepekatan larutan lanit
Volume of urine collected //
Volume of water reabsorbed //
Percentage of water
reabsorbed
Isi padu air kencing yang
dikumpul //
Isi padu air yang diserap
semula //
Peratus air yang diserap
semula
100%
Volume of drinking water
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100%
Isi padu air minuman
Constant variable:
Fix the volume of drinking water at 500ml
Pemboleh ubah dimalarkan:
Tetapkan isi padu air minuman pada 500ml
Volume of drinking water / Time Fix the time duration at 30 minutes
Tetapkan masa yang diambil pada 30 minit
duration for the urine collected
Isi padu air minuman / Masa
Fix the student
yang diambil sebelum
Tetapkan pelajar
mengumpulkan air kencing
(d) 1. As the concentration of drinking water increases, the volume of urine produced decreases /
the volume of water reabsorbed increases.
Semakin meningkat kepekatan air minuman, semakin rendah isi padu air kencing yang
dihasilkan / isi padu air yang diserap semula meningkat.
2. The higher the concentration of drinking water, the lower volume of urine produced / the
higher the percentage of water reabsorbed.
Semakin tinggi kepekatan air minuman, semakin rendah isi padu air kencing yang
dihasilkan / semakin tinggi peratus air yang diserap semula.
(e)
(i)
Volume of drinking
Volume of urine
Percentage of water
Concentration of
water intake
collected
reabsorbed by kidney
drinking water
Isi padu air
Isi padu air
Peratus air yang
Kepekatan air
minuman yang
kencing yang
diserap semula oleh
minuman
diambil
dikumpulkan
ginjal
(%)
(ml)
(ml)
(%)
0.0
500
94
81.2
5.0
500
62
87.6
10.0
500
46
90.8
(ii)
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The higher concentration intake of drinking water, the higher the percentage of water
reabsorbed.
Semakin meningkat kepekatan air minuman yang diambil, semakin meningkat peratus air
yang diserap semula.
This is because the osmotic pressure increases.
Ini adalah kerana tekanan osmotik meningkat.
Therefore secretions of ADH // permeability of kidney tubule towards water // volume of
water reabsorb increases.
Rembesan ADH // ketelapan ginjal terhadap air / isi padu air yang diserap semula
meningkat.
(g) Osmoregulation is a process of maintaining osmotic pressure / concentration of salt and water
in blood which can be determined by volume of urine collected / percentage of water
reabsorbed that is influenced / affected by concentration of drinking water.
Osmoregulasi ialah proses mengawal atur tekanan osmotik / kepekatan garam dan air dalam
darah yang boleh ditentukan oleh isi padu air kencing yang dikumpul / peratusan air yang
diserap semula yang dikawal / pengaruhi oleh kepekatan air minuman.
(h) The volume of urine collected will be more than 94ml because the caffeinated drink slowed
down the transmission of impulses through synapse // Therefore secretions of ADH //
permeability of kidney tubule towards water // volume of water reabsorbed decreases.
Isi padu air kencing yang dikumpul lebih dari 94ml kerana minuman yang mengandungi kafein
akan melambatkan penghantaran impuls melalui sinaps // Perembesan ADH // ketelapan tubul
ginjal terhadap air / isi padu air yang diserap semula menurun.
(i)
Variables
Apparatus
Materials
Pemboleh ubah
Radas
Bahan
Manipulated variables
Drinking cup
Sodium Chloride solution
Pemboleh ubah dimanipulasi
Cawan minum
Larutan natrium klorida
Responding variables
Measuring cylinder
Urine collected
Pemboleh ubah bergerak balas Silinder penyukat
Air kencing yang dikumpul
Controlled variables
Stopwatch
Student
Pemboleh ubah dimalarkan
Jam randik
Murid
(f)
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(a) (i)
State two different observations from the experiment based on the results shown in Table 1.
Nyatakan dua pemerhatian yang berbeza daripada eksperimen berdasarkan keputusan
daripada Jadual 1.
Observation 1 / Pemerhatian 1:
____________________________________________________________________
(ii)
Observation 2 / Pemerhatian 2:
____________________________________________________________________
[3 marks]
State the inferences which correspond to the observations in 1(a)(i).
Nyatakan inferens yang sepadan dengan pemerhatian di 1(a)(i).
Inference from observation 1 / Inferens daripada pemerhatian 1:
____________________________________________________________________
(b)
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Variables
Pemboleh ubah
Manipulated variable
Pemboleh ubah dimanipulasikan
______________________________
______________________________
Responding variable
Pemboleh ubah bergerak balas
______________________________
______________________________
Constant variable
Pemboleh ubah dimalarkan
______________________________
______________________________
Table 2 / Jadual 2
[3 marks]
State the hypothesis for this experiment.
Nyatakan hipotesis bagi eksperimen ini.
____________________________________________________________________
[3 marks]
(d) (i) If the allele of red flower (R) is dominant to the allele of white flower (r), record the genotypes
and phenotypes of all the draws in Table 1.
Jika alel untuk warna bunga merah (R) adalah dominan kepada alel warna bunga putih (r),
rekodkan genotip dan fenotip semua cabutan dalam Jadual 1.
[3 marks]
(ii) Using the data from Table 1, calculate the ratio of the phenotypes obtained from this
experiment.
Menggunakan data di Jadual 1, hitung nisbah fenotip yang diperolehi daripada eksperimen ini.
[3 marks]
(e) Explain the results obtained in 1(d)(ii) by using a genetic diagram.
Terangkan keputusan yang didapati di 1(d)(ii) dengan menggunakan gambar rajah genetik.
[3 marks]
(f) Based on Table 1, explain the relationship between the number of draws and the phenotype
ratio.
Berdasarkan Jadual 1, terangkan hubungan antara bilangan cabutan dengan nisbah fenotip.
______________________________________________________________________
[3 marks]
(g) Based on the experiment in the inheritance of colours in a flowering plant that the students
had done, deduce Mendels First Law of Inheritance.
Berdasarkan kepada eksperimen perwarisan warna bunga yang telah dijalankan oleh pelajarpelajar, rumuskan Hukum Mendel Yang Pertama.
______________________________________________________________________
[3 marks]
(h) The experiment is repeated by decreasing the number of draws from the pouches.
Predict the ratio of the phenotypes that you would obtain. Explain your prediction.
Eksperimen diulang dengan mengurangkan jumlah cabutan butang daripada uncang.
Ramalkan nisbah fenotip yang akan didapati. Terangkan ramalan anda.
______________________________________________________________________
[3 marks]
(c)
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(i)
Classify the list of materials shown below according to their function in the experiment.
Kelaskan senarai bahan di bawah mengikut fungsi dalam eksperimen.
Material
Bahan
Red button / Butang merah
White button / Butang putih
Pouch A and B
Uncang A dan B
Pair of buttons drawn
Pasangan butang yang dicabut
Function
Fungsi
[3 marks]
Suggested Answer:
(a)
(i)
(ii)
1. In the first draw, a red button is drawn from pouch A and a white button is drawn from
pouch B.
2. In the fifth draw, a white button is drawn from each bag. (Students can pick any pair from
the table but the two pairs must be of different combinations)
1. Pouches A and B contain the same number of red and white buttons, each button has the
same chance of being drawn.
2. The draw is made randomly and every button has the same chance of being drawn.
(b)
Variable
Manipulated variable:
Number of draws
Responding variable:
Combination of colours of the button
drawn
Constant variable:
The number of red and white buttons /
take one button from each pouch each
time
(c)
(d)
(i)
1. No matter what is the number of draws, the ratio of the phenotype always 3 red flowers to
1 white flower.
2. After twenty draws the ratio of the phenotypes must be 3 red flowers to 1 white flower.
Genotype
Rr
Rr
Rr
Rr
rr
Rr
RR
RR
rr
rr
Phenotype
Red
Red
Red
Red
White
Red
Red
Red
White
White
Genotype
Rr
RR
rr
Rr
rr
Rr
Rr
RR
RR
Rr
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Phenotype
Red
Red
White
Red
White
Red
Red
Red
Red
Red
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(ii)
(e)
(f)
(g)
(h)
1. The bigger the number of draws, the more likely it is to get the phenotypic ratio of 3:1
2. The smaller the number of draws, the less likely it is to get the phenotypic ratio of 3:1
The colours of flower is controlled by a pair of alleles represented by R, for red button and r,
for white button. During the formation of gamete, the pair of red and white buttons (Rr) will be
separated only one of the buttons // only R allele or r allele will be found / carried in each
gamete.
The phenotypic ratio will not be / will not likely be 3:1
F: The less accurate result is due to
E: the lesser draws of the button.
(i)
Material
Red button
White button
Pouch A and B
Pair of button drawn
Function
The red alleles
The white allele
Nucleus / chromosome
Genotype / phenotype of offspring
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