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INTRODUCTION
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a Grain Research Laboratory, 1404-303 Main Street, Winnipeg, MB, R3C 3G8,
Canada
b Agriculture and Agri-Food Canada, Lacombe Research Centre, 6000 C and E
Trail, Lacombe, AB, T4L 1 W1, Canada
c Agriculture and Agri-Food Canada, Lethbridge Research Centre, 5403 1st
Avenue South, Lethbridge, AB, T1J 4B1, Canada
d Alberta Agriculture and Rural Development, 5401 1st Avenue South, Lethbridge,
AB, T1J 4V6, Canada
e Alberta Agriculture and Rural Development, 5030 50th Street, Lacombe, AB,
T4L 1 W8, Canada
f Agriculture and Agri-Food Canada, Indian Head Research Farm, Box 760, Indian
Head, SK, S0G 2K0, Canada
g Agriculture and Agri-Food Canada, Scott Research Farm, PO Box 10, Scott, SK,
S0K 4A0, Canada
h Agriculture and Agri-Food Canada, Brandon Research Centre, PO Box 1000A,
Brandon, MB, R7A 5Y3, Canada
Effects of seeding rate, nitrogen rate and cultivar on barley malt quality
problems in the brewery such as slow wort and beer filtration
times and poor fermentation.5,6 Protein must also be hydrolysed
to allow access to the granules and in addition release the free
amino acids essential for fermentation. Endosperm modification
can be restricted in plump barley, because large kernels take water
up more slowly3 and contain higher levels of grain -glucan.7
Modification can also be restricted in grain with higher levels of
protein owing to slow water uptake and uneven processing.3,8
Kernel plumpness and grain protein levels are affected by crop
management practices such as seeding rate, nitrogen fertilisation
rate and cultivar selection. Higher seeding rates reduce kernel
plumpness and, although grain protein level is also reduced,
it is generally accepted that a lower malt extract level will
result.9 Higher seeding rates, however, also reduce variability in
kernel size,10,11 which can result in a more consistent endosperm
modification.12 Increasing the nitrogen fertiliser rate, necessary
for maximum barley yields, results in higher grain protein levels,
thinner kernels and greater variability in kernel size.10 Acceptable
levels of barley protein have been achieved at high fertilisation
rates with breeding lines, but malt extract levels decreased
significantly.2 Commercial barley cultivars also differ in their
response to nitrogen fertilization, as seen with CDC Copeland and
AC Metcalfe,10 but effects on malt quality are not well understood.
Crop management studies for barley seldom determine malt
quality directly owing to the expense and time requirements
for malting and malt analysis. Comments on malt quality tend
to be extrapolated from barley data or based on previous
experiences. The present study, an extension of our previous
publication that reported on effects of crop management on barley
quality,10 investigated malt quality directly. The study investigated
seeding rates, nitrogen fertilisation rates, differences between two
commonly grown Canadian malting cultivars and interactions
among these treatments.
from germinative energy results according to Riis and BangOlsen.16 A Single Kernel Characterization System (SKCS 1400,
Perten Instruments, Springfield, IL, USA) was used to measure
kernel weight, diameter and hardness.17 The system calculated
averages for weight, diameter and hardness of 300 barley kernels
together with their respective standard deviations, the latter being
used as an indicator of kernel uniformity.
Micromalting process and malt analyses
Barley samples from all growing locations were tested for
plumpness, germinative energy and protein content to determine
suitability for malting. Constraints on capacity for malting and
quality analysis limited the number of locations that could be
malted and analysed each year. Selection of locations was based
on barley quality (grain protein and germination). Barley from a
total of 16 location/years was malted. Barley from two locations
(Beaverlodge and Scott) was only malted from one year. Barley
from all other locations was selected for multiple years. The
number of replicates malted for each selected location/year varied
but generally was greater than two. A total of 39 replicates spread
across the 16 location/years were malted (780 samples).
Only plump barley (>2.38 mm slotted sieve) was malted
in a Phoenix Automated Micromalting machine (Adelaide, SA,
Australia) using the following malting schedule: steeping (8 h
wet,16 h air, 8 h wet, 12 h air at 13 C), germination (96 h at 15 C)
and kilning (12 h at 55 C, 6 h at 65 C, 2 h at 75 C, 4 h at 85 C 24 h
total). Steep-out moisture was calculated from the difference in
weight between dry matter barley and steeped-out barley. Malt
analyses included: (1) malt extract (fine grind), a measurement
of the solubility of malt that indicates a malts beer production
potential; (2) Kolbach index, the ratio of soluble to total malt
protein that indicates the extent of protein modification; (3) wort glucan, an indicator of the extent to which cell walls were degraded
during malting; (4) diastatic power and -amylase, enzymes that
produce fermentable sugars from malt starch during mashing,
the first phase of brewing. Analyses were performed according
to the standard methods of the American Society of Brewing
Chemists.15 Malt modification and homogeneity of modification
were assayed with both the friability method15 and the Calcofluor
staining method.18
Statistical model and analyses
Data were analysed using PROC MIXED of SAS.19 Seeding rate,
nitrogen rate and cultivar were considered fixed effects. Location
by year combinations (environments) and their associated
interactions with fixed effects were considered random effects,
as were replicates within environments. Yang20 suggests that in
breeding and agronomic studies it may be more appropriate to
consider year and location effects and their interactions with fixed
effects as random, since the goal of most crop improvement
programmes is to infer future performance at many untested
locations. The large number of environments investigated in this
study facilitated the use of this approach.
Barley cultivar and seeding rate means were compared using
Fishers protected least significant difference (LSD) test. Contrast
statements were used to test for linear and quadratic responses
to nitrogen rate. The mixed model was used to obtain regression
equations to describe relationships between nitrogen rate and
dependent variables. All differences were deemed significant at
< 0.05.
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RESULTS
The average quality of barley from the 16 location/years selected
for malting was appropriate for end use malting. Average grain
protein level (dry matter, DM) for all malted samples was
111 15 g kg1 (mean standard deviation). Average kernel
plumpness was 928 50 g kg1 . The average 4 day germination
was 98 2%. Barley showed some water sensitivity, but the average
value (92 9%) was well above the commercially acceptable level
of 80%. Malts produced from barley grown at the 16 location/years
were reasonably well modified, as indicated by low wort -glucan
levels (124 90 mg L1 ) and high Kolbach indices (42.7 4.0%).
Malts had good levels of malt extract (805 11 g kg1 ) and
starch-degrading enzymes, the latter indicated by diastatic power
(132 27 Lintner) and -amylase (58.0 11.0 dextrinising units).
Effect of seeding rate
Seeding rate affected several aspects of barley and malt quality
(Tables 1 and 2). Barley grown at the high seeding rate had lower
levels of grain protein, greater potential for a rapid initiation to
germination (germination index) and higher steep-out moistures
(Table 3). Malts produced from high-seeding-rate barley had better
Table 1.
MJ Edney et al.
endosperm modification, as indicated by lower levels of wort glucan, 112 vs 143 mg L1 , higher Kolbach indices, 42.9 vs 42.0%,
and higher values for both friability and Calcofluor. Malts made
from the high-seeding-rate barley showed slightly higher levels
of malt extract, 806 vs 805 g kg1 DM, but lower seeding rates
resulted in higher malt yields, 921 vs 920 g kg1 . Levels of starchdegrading enzymes were similar between the two seeding rates,
although malt from the high seeding rate had more -amylase
while malt from the low seeding rate had more diastatic power.
Effect of nitrogen rate
Increasing levels of nitrogen fertilisation affected nearly all aspects
of malt processing and malt quality (Tables 1 and 2). Grain protein
levels increased with increasing nitrogen rate, while germinative
energy showed a parabolic relationship (Fig. 1). Germination index
and steep-out moistures decreased with increasing nitrogen and,
as a result, endosperm modification decreased, as indicated by
increasing levels of wort -glucan, lower Kolbach indices (Fig. 2)
and lower values for both friability and Calcofluor (Fig. 3). Malt
extract levels decreased with increasing levels of nitrogen (Fig. 4),
but, as expected, levels of diastatic power and -amylase increased
(Fig. 2). Malt yields were unaffected by nitrogen rate.
P values from analysis of variance for fixed effects of barley cultivar, seeding rate and nitrogen rate on grain and malt processing variables
Grain hardness
Effect
Cultivar (C)
Seeding rate (S)
CS
Nitrogen rate (N)
N linear
N quadratic
CN
SN
CSN
Environment interactionb
Barley
protein
Average
Standard
deviation
Germinative
energy
Water
sensitivity
Germination
index
Steep-out
moisture
Malt
yield
<0.001a
<0.001
0.418
<0.001
<0.001
<0.001
0.335
0.779
0.662
15
<0.001
0.301
0.031
0.035
0.819
0.428
0.448
0.903
0.976
24
0.774
0.124
0.903
<0.001
<0.001
0.040
0.914
0.977
0.996
18
0.022
0.002
0.247
0.111
0.795
0.007
0.826
0.400
0.660
18
<0.001
0.617
0.806
0.850
0.994
0.302
0.968
0.781
0.510
25
<0.001
<0.001
0.656
0.040
0.011
0.934
0.788
0.688
0.269
4
<0.001
<0.001
0.894
<0.001
<0.001
0.093
0.360
0.277
0.365
7
<0.001
0.005
0.575
0.023
0.031
0.011
0.346
0.908
0.330
1
Table 2.
P values from analysis of variance for fixed effects of barley cultivar, seeding rate and nitrogen rate on malt quality variables
Malt
extract
Soluble
protein
Kolbach
index
Wort
-glucan
Diastatic
power
-Amylase
Friability
modification
Calcofluor
modification
Calcofluor
homogeneity
0.010a
0.009
0.156
<0.001
<0.001
<0.001
0.018
0.538
0.335
14
<0.001
0.668
0.787
<0.001
<0.001
0.008
0.657
0.977
0.109
14
<0.001
<0.001
0.913
<0.001
<0.001
0.006
0.958
0.994
0.289
16
0.678
<0.001
0.011
<0.001
<0.001
<0.001
0.953
0.956
0.806
9
<0.001
<0.001
0.929
<0.001
<0.001
<0.001
0.692
0.531
0.175
10
<0.001
0.004
0.389
<0.001
<0.001
0.329
0.425
0.812
0.843
14
<0.001
<0.001
0.179
<0.001
<0.001
<0.001
<0.001
0.262
0.843
18
<0.001
<0.001
0.537
<0.001
<0.001
0.399
0.926
0.951
0.893
NDc
0.222
<0.001
0.934
<0.001
<0.001
0.549
0.908
0.418
0.933
ND
Effect
Cultivar (C)
Seeding rate (S)
CS
Nitrogen rate (N)
N linear
N quadratic
CN
SN
CSN
Environment interactionb
a,b
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See Table 1.
Not determined.
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Effects of seeding rate, nitrogen rate and cultivar on barley malt quality
Table 3.
Parameter
Barley protein (g kg1 DM)a
Average grain hardness (SKCS units)
Standard deviation grain hardness
Germinative energy (%)
Water sensitivity (%)c
Germination index
Steep-out moisture (g kg1 )
Malt yield (g kg1 )
Fine grind malt extract (g kg1 DM)
Soluble protein (g kg1 DM)
Kolbach index (%)
Wort -glucan (mg L1 )
Diastatic power ( L)d
-Amylase (DU)e
Friability modification (%)
Calcofluor modification (%)
Calcofluor homogeneity (%)
LSD
200 seeds 400 seeds
m2
(P < 0.05)
m2
114b
51.5
14.14
97.9
93.1
6.9
456
921
805
48.2
42.0
143
133
57.4
78.7
92.1
83.5
111
51.1
14.27
98.3
92.8
7.3
459
920
806
48.3
42.9
112
129
58.2
81.6
93.9
85.4
1.2
0.79
0.17
0.30
1.05
0.09
0.9
0.84
1.0
0.4
0.36
8.07
2.01
0.56
1.00
0.62
0.78
Effect of cultivar
Several aspects of barley and malt quality were affected by
cultivar (Tables 1 and 2). CDC Copeland had lower protein than AC
Metcalfe, 109 vs 116 g kg1 DM, slightly better germinative energy,
98.2 vs 97.9%, and less water sensitivity, 94.2 vs 91.5% (Table 4). AC
Metcalfe kernels initiated germination more rapidly, as indicated
by higher germination indices, 7.37 vs 6.88, which contributed to
higher steep-out moistures, 459 vs 456 g kg1 , even though AC
Metcalfe had harder kernels, 56.6 vs 46.1 SKCS units.
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DISCUSSION
Environment was a significant source of variance for most barley and malt parameters analysed (data not shown). However,
the objective of the study was to determine how seeding rate,
nitrogen rate and cultivar affected malt quality, and not effects of environment on quality, which have been previously
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Figure 1. Effect of nitrogen rate on (A) grain protein, (B) germinative energy, (C) germination index and (D) steep-out moisture. Symbols represent data
averaged over all environments. Lines were derived from regression coefficients as calculated with mixed model analysis.
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MJ Edney et al.
Figure 2. Effect of nitrogen rate on (A) wort -glucan, (B) -amylase, (C) Kolbach index and (D) diastatic power. Symbols represent data averaged over all
environments. Lines were derived from regression coefficients as calculated with mixed model analysis.
Figure 3. Effect of nitrogen on (A) friability modification, (B) friability homogeneity, (C) Calcofluor modification and (D) Calcofluor homogeneity. Symbols
represent data averaged over all environments. Lines were derived from regression coefficients as calculated with mixed model analysis.
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documented.1,2 Consistency of treatment effects across environments (locations/years) was a concern and was indicated by the
percentage of environment variance associated with the sum
of environment/treatment interactions (Tables 1 and 2). The percentage was low (<10%) for the malt processing parameters,
germination index, steep-out moisture and malt yield but higher
(1520%) for barley parameters such as grain protein, grain hardness, germinative energy and water sensitivity (Table 1). The
percentage for malt quality parameters averaged nearly 16%.
All percentages of environment/treatment interaction variance
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were still relatively low compared with the overall variances associated with the environment, indicating that treatment effects
were relatively consistent across environments.
Seeding rates and nitrogen fertilisation rates are known to
affect barley quality, but effects on malt quality have often only
been surmised from the barley data. However, the endosperm
modification so necessary for good malt quality is a complex
process dependent on all aspects of barley quality, including their
interactions. Malt quality, therefore, is best determined directly if
Effects of seeding rate, nitrogen rate and cultivar on barley malt quality
Table 4.
Parameter
Barley protein (g kg1 DM)a
Average grain hardness
(SKCS units)
Standard deviation grain
hardness
Germinative energy (%)
Water sensitivity (%)c
Germination index
Steep-out moisture (g kg1 )
Malt yield (g kg1 )
Fine grind malt extract
(g kg1 DM)
Soluble protein (g kg1 DM)
Kolbach index (%)
Wort -glucan (mg L1 )
Diastatic power ( L)d
-Amylase (DU)e
Friability modification (%)
Calcofluor modification (%)
Calcofluor homogeneity (%)
ae
AC
Metcalfe
CDC
Copeland
LSD
(P < 0.05)
116b
56.6
109
46.1
1.2
0.79
14.19
14.22
0.17
97.9
91.5
7.37
459
919
806
98.2
94.2
6.88
456
922
805
0.30
1.05
0.08
0.9
0.8
1.0
48.9
42.1
127
146
66.2
76.3
93.8
84.2
47.6
42.9
128
116
49.4
83.9
92.2
84.7
0.4
0.36
8.1
2.00
0.56
1.03
0.62
0.78
See Table 3.
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Table 5. Effects of interaction between barley cultivar and seeding rate (low, 200 seeds m2 ; high, 400 seeds m2 ) on kernel diameter, kernel
hardness, wort -glucan and malt extract
Cultivar
AC Metcalfe
CDC Copeland
Low rate
High rate
Low rate
High rate
Low rate
High rate
Low rate
High rate
2.56a
2.56
2.53
2.50
56.3
46.8
56.8
45.5
136
149
117
108
806
804
806
805
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quality of higher nitrogen rates can be reduced by breeding and
growing cultivars that achieve better protein modification during
malting.
ACKNOWLEDGMENTS
This research was funded by grants from the Alberta Barley
Commission, the Canadian Wheat Board, RAHR Malting and
the Matching Investment Initiative of Agriculture and Agri-Food
Canada. The excellent technical assistance of the following is
gratefully appreciated: Len Dushnicky, Jason Herman, Justin
Highmoor, April Johnson, Anders Leung, Shirley Lowe, Marnie
MacLean, Aaron MacLeod, Ashley Orr, Shawn Parsons, Mary
Richardson and Mike Svistovski.
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MJ Edney et al.
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