B.tech Biotechnology

B.
TECH
BIOTECHNOLOGY
Department of Biotechnology,
Manipal Institute of Technology (MIT),
Manipal University,
Manipal
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B.E. BIOTECHNOLOGY (200 Credits)
IV YEAR
III YEAR
II YEAR
Yr.
Sub
Code
MAT201
BIO 201
BIO 203
BIO 205
BIO 207
BIO 209
ODD SEMESTER
Subject Name
Mathematics III
Introduction to Bioprocess Engineering.

Process Biochemistry
Industrial Microbiology
Process and Bioprocess Calculations
Fluid flow operations in Bioprocessing
4
4
4
3
3
0
0
0
1
1
0
0
0
0
0
4
4
4
4
4
Sub
Code
MAT
202
BIO 202
BIO 204
BIO 206
BIO 208
*** ***
BIO 211
Biochemistry Lab
BIO 210
BIO 213
Microbiology Lab
Total
0
22
0
2
3
6
1
26
BIO 212
BIO 301
Chemical & Biochemical Engg. Thermodynamics
BIO 303
BIO 305
BIO 307
BIO 309
BIO 311
BIO 313
BIO 315
BIO 317
Fermentation Engineering
Downstream Processing in Biotechnology-II
Computational techniques in Bio Technology
Mass Transfer Operations in Bioprocessing
Bioprocess and Bio-Reaction Engineering
Downstream Processing Lab
Fermentation Engineering lab
Computational techniques in Bio Technology - Lab
Total
Animal and Plant Biotechnology
Process Engineering Economics and Optimization
Modelling and Simulation in Bio- process Engineering
3
3
3
3
3
0
0
0
19
4
3
3
3
3
3
0
1
1
0
1
1
0
0
0
4
0
1
1
0
0
0
1
0
0
0
0
0
3
3
3
9
0
0
0
0
0
0
3
4
4
3
4
4
1
1
1
26
4
4
4
3
3
3
2
19
26
BIO 401
BIO 403
BIO 405
BIO ***
BIO ***
BIO ***
BIO 407
BIO 409
BIO 411
Program Elective-II
Program Elective -III
Program Elective- IV
Process Control Lab
Modeling and simulation in Bioprocess Engineering
Lab
Seminar
Total
Page 3 of 73
HUM
302
BIO 302
BIO 304
BIO 306
BIO ***
*** ***
BIO 308
BIO 310
BIO 312
BIO 402
BIO 499
EVEN SEMESTER
Subject Name
Mathematics IV
Cell and Molecular Biology

Genetic engineering
Downstream Processing in Biotechnology-I
Heat Transfer operations in Bioprocessing
Open Elective I
Molecular Biology & Genetic Engineering
Lab
Unit Operations Lab
Total
Essentials of Management & Engineering
Economics
Bioinformatics
Bioprocess Control
Bioprocess Equipment Design
Program Elective- I
Open Elective II
Bioinformatics Lab
Bioprocess and Bioreaction Engineering Lab
Bioprocess Equipment Drawing
Total
Industrial Training/ Tour
Project Work/ Practice School
4
4
3
3
3
0
0
1
1
0
0
0
0
0
0
4
4
4
4
3
0
21
0
3
3
6
1
26
3
3
3
3
3
0
0
0
19
0
0
1
1
1
0
0
0
0
0
3
0
0
0
0
0
0
0
3
3
3
9
0
0
4
4
4
3
3
1
1
1
25
1
20
21
Total
BIO 320 Immunotechnology
BIO 421 Biological industrial waste water
BIO 429 Multiphase Bioreactor design
Engineeing
BIO 437 Drugs and Pharmaceutical

Biotechnoloy
BIO 322 Food processing Technology
BIO 423 Protein Engineering
BIO 431 Genomics & Proteomics
BIO 439 Solid state fermentation
BIO 324 Bioethics & Biosafety
BIO 425 Advanced Bioprocess Engg
BIO 433
BIO 441 Solid waste management
BIO 326 Bioremediation
BIO 427 Molecular Modeling & Drug design
BIO 435 Metabolic Engineering
OPEN ELECTIVES:
BIO 316: Introduction to Genetics

BIO 318: Industrial Biotechnology
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Biopolymer technology
BIO 443 Biofuels Engineering
B.E - BIOTECHNOLOGY
THIRD SEMESTER
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MAT 201
Total No. of hours: 48
MATHEMATICS III
[4 0 0 4]
Fourier series, Periodic Functions, Euler's formulae. Fourier series of odd and even functions
with arbitrary period. Half range expansions, Fourier sine and cosine transforms and Fourier
Integrals.
[12]
Partial differential equations: basic concepts, solutions of equations involving derivatives
with respect to one variable only. Solutions by indicated transformations and separation of
variables.
[03]
Derivation of one dimensional wave equation (vibrating string) and its solution by using the
method of separation of variables. Simple problems. D'Alembert's solution of wave equation.
Derivation of one dimensional heat equation using Gauss divergence theorem and solution of
one dimensional heat equation. Solution by separation of variables.
[05]
Interpolation and applications -Finite differences, Newton-Gregory and Lagrange's
interpolation formulae, Inverse interpolation, Numerical differentiation.
[10]
Numerical Integration -Trapezoidal and Simpson's 1/3 and 3/8th rule.
[03]
Bessel and Legendre's equations, Solution, orthogonal properties. Data analysis, curve fitting
and regression analysis.
[15]
Text / Reference Books:
Erwin Kreyszig. 1985. Advanced Engg. Mathematics.Wiley Eastern.
S.S.Sastry. 1990. Introductory Methods of Numerical Analysis. Prenctice Hall.
B.S.Grewal. Higher Engg.Mathematics, Khanna Publishers
Gerald C.F. & Partrick D.Wheatley. 1984. Applied Numerical Analysis. Addison
Wesley.
Francis Scheid. 1968. Numerical Analysis. Schaum Series, McGraw Hill.
M.K.Jain and S.R.K.Iyengar and R.K.Jain. 1985. Numerical Methods for Scientific
and Engineering Computations. Wiley Eastern.
Conte S.D and Carl De Boor. 1972. Elementary Numerical Analysis. McGraw Hill.
Page 6 of 73
BIO 201
INTRODUCTION TO BIOPROCESS ENGINEERING
Total number of periods: 48
[4 0 0 4]
Introduction to Bioprocess
[05]
Historical development of bioprocess technology; an overview of traditional & modern
applications of biotechnological processes; role of a bioprocess engineer in the biotechnology
industry; outline of an integrated bioprocess; the various (upstream & downstream) unit
operations involved in bioprocesses; generalized process flow sheets.
Fermentation process
[05]
General requirements of fermentation processes; basic design & construction of fermentor &
ancillaries; main parameters to be monitored & controlled in fermentation processes; an
overview of aerobic & anaerobic fermentation processes and their application in the
biotechnology industry; solid-substrate fermentation and its applications.
Metabolic stoichiometry & energetics
[10]
Stoichiometry of cell growth & product formation elemental balances; degrees of reduction
of substrate & biomass; available-electron balances; yield coefficients of biomass & product
formation; maintenance coefficients. Energy analysis of microbial growth & product
formation oxygen consumption & heat evolution in aerobic cultures.
Introduction to Enzymes
[05]
Nature and function of enzymes, coenzyme and cofactor, classification of enzymes, assay
methods and units and introduction to immobilization.
Industrial uses of enzymes
[05]
Enzymes used in detergents, use of proteases in food, leather and wool industries; uses of
lactase in dairy industry, glucose oxidase and catalase in food industry, medical application
of enzymes.
Enzyme catalyzed reactions
[10]
Michaelis-Menten Equation mechanism, derivations, types of enzyme inhibition, kinetics,
effect of environmental factors on enzyme activity.
Introduction to microbial kinetics
[08]
Microbial Cell cultivation, Nutrient media, enrichment culture, culture production and
preservation effect of environmental factors on micro organism growth. Cell Growth
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Measurements, Growth Cycle for Batch cultivation, Microbial cell kinetics - Introduction to
various types of Bioreactors their industrial applications.
Textbook:
Michael L Shuler and Fikret Kargi. 2008. Bioprocess Engineering: Basic Concepts.
Prentice-Hall of India Pvt Ltd.
Reference Books:
Pauline M. Doran. 1995. Bioprocess Engineering Principles. Academic Press.
Harvey W.Blanch and Douglas S. Clark. 1997. Biochemical Engineering CRC Press
S.Aiba et al. 1973. Biochemical Engineering. Academic Press.
B. Atkinson. 1974. Biochemical Reactors, Pion Ltd.
Page 8 of 73
BIO 203
Total No. of periods: 48
PROCESS BIOCHEMISTRY
[4 0 0 4]
Introduction to Biomolecules:
Structure and properties of Mono-, Di- and Poly-saccharides, structure and properties of fatty
acids, neutral fats, phospholipids, glycolipids and steroids; structure and properties of
aminoacids, peptides and proteins. Biologically important peptides. Structure and properties
of purines, pyrimidines, nucleosides, nucleotides. Nucleoprotein complexes. Brief
introduction of mRNA, tRNA and genetic code
[12]
Metabolism:
Glycolysis, Aerobic, Anaerobic, TCA cycle, gluconeogenesis. HMP shunt pathway, pentose
phosphate pathway, interconnection pathway, metabolic regulation. Biosynthesis of
polysaccharides, glucose from simple precursors. Generation of ATP in anaerobic cell,
balance sheet of glycolysis. Intracellular organization of glycolytic system.
[12]
Biosynthesis of fatty acids, cholesterol, phospholipids, glycolipids, Eicosanoids
(prostaglandin, leucotrienes, thromboxane), biodegradation of fatty acids - alpha, beta,
omega oxidations.
[10]
Biosynthesis of aminoacids, biodegradation of aminoacids, glutamine, glutamine acid
pathway, de-amination and trans-amination, urea cycle.
[08]
Biosynthesis and biodegradation of purines and pyrimidines. Molecular diseases: Inborn
errors of metabolism, disorders.
[06]
Textbook:
Albert Lehninger. 1996. Principles of Biochemistry. CBS Publishers.
Reference Books:
Lubert Stryer. 2007. Principles of Biochemistry. Freeman.
Voet and Voet. 2005. Biochemistry. Wiley.
Trehan K. 1990. Biochemistry. New Age International.
Page 9 of 73
BIO 205
INDUSTRIAL MICROBIOLOGY
Total No. of hours: 48
[4 0 0 4]
Introduction: Microbial diversity and taxonomy, Prokaryotes and Eukaryotes, Types of

Microorganisms.
[2]
Microbiological Techniques: Study of microscopes, Sterilization Techniques Heat, steam,
radiation, filtration and chemical.
[3]
Structure, Functions and Replication of Microorganisms: Bacteria, Viruses, Fungi, Algae,
Protozoans Helminthes
[15]
Microbial Metabolism: Important aspects of metabolic pathways and bioenergetics, aerobic
and anaerobic growth.
[5]
Medical Microbiology: Common diseases caused by microbes (brief discussion): Bacteria
Tuberculosis, Leprosy, Typhoid, Diarrhoea, Syphilis, Pneumonia. Viruses -AIDS, Hepatitis,
Polio, Rabies. Fungi Candidiasis. Protozoans Ameobiosis, Malaria, Leishmaniasis.
[6]
Microbial insecticides: Bacillus thuringinesis, Sphaericus, Popilliae.
[3]
Microbial enzymes: Production of Microbial enzymes, strain, medium, fermentation
processes. Large-scale application of Microbial enzymes starch processing, textile,
detergents, cheese industry.
[4]
Microbial polysaccharides: Examples; Xanthan: structure and applications.
[2]
Food industry: Microbial spoilage of food and its control; food preservatives; single cell
protein (SCP) and single cell oil (SCO); food borne infections and their control.
[5]
Biofertilizers: Nitrogen fixing organisms.
Text Books:
Pelczar, Chan and Kreig. 1998. Microbiology. W C Brown Pub
Presscot and Dunn. 2004. Industrial Microbiology. CBS Publishers.
Reference Books:
Stanier, Ingraham and Wheeler. 1994. General Microbiology. McMillan.
Page 10 of 73
[3]
BIO 207
PROCESS AND BIOPROCESS CALCULATIONS
Total No of periods: 48
[3 1 0 4]
Units and dimensions, conversion of units, physical and chemical properties of compound
and mixtures, Techniques of problem solving, chemical equation and stoichiometry
(08)
Phase equilibrium, Vapour pressure, Gibbs phase rule; liquid system, ideal and real gases,
Raoults and Henrys law, simple humidity based calculations
(06)
Steady state material balances: Program of analysis of material balance problems; material
balance for various unit operations; graphical methods to solve material balance- binary and
ternary, material balance involving multiple sub-system
(10)
Material balance with chemical reactions; Material balance for systems involving recycle,
bypass and purge calculations
(10)
Introduction to unsteady state material balance- simple models
(04)
Stoichiometry of microbial growth and product formation, problems based on yield

coefficients, respiration quotients, & reduction ratio
(04)
Energy and energy balances: Balances on non-reactive process; Heat of mixing and solution;
balance on reactive processes; calculations of heats of reaction; formation and combustion,
adiabatic reaction temperature
(06)
David M.Himmelblau. 1989. Basic Principles and Calculations in Chemical

Engineering. Prentice Hall of India (P) Ltd.
A.Hougen, K.M.Watson and R.A.Ragatz. 1970. Chemical Process Principles, Part I, John Wiley and Asia Publishing Co.
Bhat B.I and S.M.Vora, 2005. Stoichiometry. Tata McGraw Hill.
Richard Felder and Ronald W.Rausseau. 1986. Elementary Principles of Chemical

Processes. John Wiley & Sons.
Anderson and Wenzel. 1961. Introduction to Chemical Engineering, McGraw Hill.
Michael Shuler and Fikret Kargi. 2002. Bioprocess Engineering: Basic Concepts.
Prentice Hall.
Pauline Doran. 1995. Bioprocess Engineering Principles. Academic Press.

Page 11 of 73
BIO 209 FLUID FLOW OPERATIONS IN BIOPROCESSING

[3 1 0 4]
Review of fluid statics hydrostatic equilibrium measurement of fluid pressure

manometers
[03]
Fluid dynamics types of flow shear stress - viscosity, classification of fluids.
[05]
Basic equations of fluid flow continuity equation, Bernoullis equation derivation and
applications
[07]
Laminar flow through non circular conduits, Reynolds number and friction factor relations
for smooth and commercial pipes. Hagen poiseuille equation derivation and applications
[14]
Flow past immersed bodies boundary layer. Types of drag, drag coefficient and stream
lining. Flow through a bed of solids derivation of Ergun, Kozeny Carmen and Blake
Plumer equation applications. Agitation and mixing of liquids.
[12]
Transportation and metering of fluid flow, pipes, pipe fittings and valves. Pumps and
compressors. Dimensional analysis and similitude
[07]
McCabe & Smith. 1993. Unit Operations of Chemical Engineering. McGraw Hill.
Coulson and Richardson. 2001. Chemical Engineering - Vol. I. ELBS
Badger and Banchero. 1995. Introduction to Chemical engineering. McGraw Hill
Foust. 2008. Principles of Unit Operations. Wiley.
Page 12 of 73
BIO 211
BIOCHEMISTRY LAB
The following experiments will be performed in the Biochemistry Lab.

1. Preparation of Buffers
2. Estimation of Glucose by DNS method
3. Estimation of Glucose by GOD/POD assay
4. Estimation of Glucose by Benedicts Titrimetric Method
5. Estimation of Starch by Anthrone test
6. Estimation of Protein by Lowrys method
7. Estimation of Protein by Bradfordss method
8. Estimation of Glycine by Sorensons method
9. Estimation of Lipids
10. Estimation of Cholesterol by Wybenga and Pileggi method
11. Estimation of Cholesterol by Zaks method
12. Estimation of Vitamin C
13. Kinetics of inverstase enzyme
14. Estimation of specific activity of salivary amylase
15. Estimation of DNA and RNA by spectro photometric method
Reference:
Albert Lehninger. 1996. Principles of Biochemistry. CBS Publishers.
Page 13 of 73
[0 0 3 1]
BIO 213
MICROBIOLOGY LAB
In the Microbiology Lab, the following experiments will be performed.

1.
2.
3.
4.
5.
6.
7.
Preparation of Liquid and Solid Media.

Pure culture Techniques - Streak, Pour and Spread Plate Methods
Standard Plate Count (SPC)
Direct Microscopic Count using a Haemocytometer
Simple Staining,Negative Staining, Gram Staining
Hot Spore Staining / Schaeffer-Fulton Method
Lactophenol Blue staining of Fungi
8. Indole Production Test

9. Methyl red Test
10. Voges Proskauer Test
11. Citrate Utilization Test
12. Catalase Test
13. Triple Sugar Iron Test
14. Casein Hydrolysis
15. Determination of Quality of Milk by Methylene Blue Reduction Test.
Reference:
Pelczar, Chan and Kreig. 1998. Microbiology. W C Brown Pub
Presscot and Dunn. 2004. Industrial Microbiology. CBS Publishers.
Page 14 of 73
[0 0 3 1]
B.E BIOTECHNOLOGY
FOURTH SEMESTER
Page 15 of 73
MAT 202
MATHEMATICS IV
[4 0 0 4]
Solution of system of linear equations, Gauss Jacobi, Gauss- Seidel and Relaxation methods.
Solution of tridiagonal systems.
[06]
Eigen values and Eigen vectors of matrices and elementary properties computation of largest
Eigen value by the power method
[04]
Numerical solution of initial value problems in ordinary differential equations by Taylor
series method, Euler's method and Runge Kutta methods of second and fourth order,
predictor corrector methods, Adams- Bashforth, Adams- Moultan and Miline methods. [08]
Introduction to probability, finite sample spaces, conditional probability and independence,
Baye's theorem, one-dimensional random variable, mean, variance, Chebyschev's inequality
[12]
Two and higher dimensional random variables, covariance, correlation coefficient,
regression, least squares principles of curve fitting .
[06]
Distributions: Binomial, Poisson, uniform, normal, gamma Chi-square and exponential,
simple problems.
[06]
Finite element method, Introduction, simple applications.
[06]
P .L.Meyer. 1979. Introduction to probability and statistical applications. American

Publishing Co.
S.S.Sastry. 1990. Introductory Methods of Numerical Analysis, Prentice Hall.
Hogg and Craig. 1975. Introduction of Mathematical Statistics. McMillan
Gerald C.F & Patrick Wheatley. 1984. Applied Numerical analysis. Addison Wesley.
M.K.Jain, S.R.K. Iyengar and R.K. Jain. 1985. Numerical methods for Scientific and
Engg. Computations. Wiley Eastern
Page 16 of 73
BIO 202
CELL AND MOLECULAR BIOLOGY
[4 0 0 4]
Cell Division:
Mitosis Mechanisms and Stages, Typical time course for mitosis and cytokinesis in animal
and plant cells. Significance, molecular organization and Functional role of mitotic
apparatus. Meiosis Mechanisms and Stages, Pairing and Synopsis, Non-disjunction. [06]
Cell cycle:
Phases of cell cycle in animals, plants and yeast, Proteins associated with cell cycle.
[03]
Cellular Biology:
Nucleus, membrane structure, membrane transport, intercellular compartments, chloroplast,
peroxisomes, ER, Golgi bodies, lysosomes, endosomes)
[03]
Classical Genetics:
Classical experiments of Hershey and Chase, Avery McLeod and McCarty, Bacterial
Conjugation, Generalised and Specialized Transduction, Transformation
[04]
Structure and Organization of Nucleic Acids:
Different forms of DNA and RNA Structures and functions, Organization of DNA in
Prokaryotic and Eukaryotic Chromosomes Histones, Cot curves
[04]
DNA Replication:
DNA Replication in Prokaryotes and Eukaryotes, -mode of Replication, Displacement (D)
Loops, Rolling Circle Method (mode) of DNA Replication, Telomeric Replication in
Eukaryotes, Replication of Viral DNA brief overview.
[04]
Transcription:
Transcription in Prokaryotes and Eukaryotes Transcription Factors, Promoters, Enhancers;
Post-transcriptional Modifications RNA Splicing; Ribozymes.
[05]
Translation:
The Genetic Code Features, Codon and Anticodon Concept, Wobble Hypothesis,
Translation in Prokaryotes and Eukaryotes, Post-translational Modifications major points.
[07]
Regulation of Gene Expression:
The Operon Concept Promoter, Operator, Terminator, Attenuator, Inducer, Repressor,
Effect of cAMP Complex; Few examples of operons lac operon.
[07]
Page 17 of 73
DNA Repair, Mutagenesis and Mutations:

Biochemical mechanisms of DNA Repair, Types of Mutations, Biochemical basis of
mutants, Modes of Mutagenesis, Reversion.
[05]
Textbook:
David Friefelder. 1987. Molecular Biology. Jones and Bartlett Publishers Inc.
Reference Books:
Benjamin Lewin. 2003. Genes VII. Oxford University Press.
James D. Watson, Michael Gilman, Jan A. Witkowski and Mark Zoller. 1992.
Recombinant DNA Technology. W. H. Freeman.
James D Watson, WH Hopkins, JW Roberts, JA Steitz and AM Weiner. 2008.

Molecular Biology of the Gene. Pearson Education Pvt Ltd
Page 18 of 73
BIO 204
GENETIC ENGINEERING
[4 0 0 4]
Basics of Recombinant DNA Technology:

[07]
Introduction to cloning, Method of creating recombinant DNA molecules, Cloning Vectors
plasmids, phages host range, Expression Vectors Prokaryotic and Eukaryotic Yeast,
Insect, Mammalian (in brief).
Enzymes in Genetic Engineering:
[07]
Restriction-Modification (RM) Systems Restriction Endonucleases and Exonucleases, Dam
and Dcm Methylases, Ligases, Terminal Transferase, Polynucleotide Kinase,
Phosphorylases, Phosphatases, RNase, DNase.
Nucleic Acid Hybridization and DNA Libraries:
[07]
The hybridization reaction, Production and Labelling of Gene Probes, Southern Blotting,
Northern Blotting, in situ hybridization, Construction of Genomic and cDNA libraries.
Molecular Analysis and Amplification Methods:
[07]
Restriction Mapping, Design of Adaptors & Linkers, Polymerases chain reaction (PCR):
Types and its applications, Restriction Fragment Length Polymorphisms (RFLP), Methods of
Nucleic Acid Sequencing Sanger Sequencing, Maxam and Gilbert Sequencing.
Applications of Recombinant DNA Technology:
[20]
Diagnostic Tools: Single Nucleotide Polymorphisms (SNPs), Variable Number Tandem
Repeats (VNTRs) Forensic application of VNTRs;
Methods of DNA Transfection to plants and animals;
New Drugs and Therapies: Therapeutic proteins from Transgenic plants and animals
(Pharming), Transgenic animals as Models of human disease, Gene Therapy;
Combating Disease: Recombinant vaccines DNA Vaccines;
Plant Breeding: Herbicide-resistance, Virus-resistance, Insect and pest-resistance, Stress
tolerance, Modification of production traits.
Text Books:
Sandy B. Primrose, Richard M. Twyman and Robert W. Old. 2002. Principles of

Gene Manipulation. Wiley-Blackwell Publishers.
Benjamin Lewin. 2003. Genes VII. Oxford University Press.
Reference Books:
J.M. Walker and E.B. Gingold. 1999. Molecular biology and Biotechnology. Panima
Publishing Corporation.
James D Watson, WH Hopkins, JW Roberts, JA Steitz and AM Weiner. 2008.
Molecular Biology of the Gene. Pearson Education Pte Ltd
T.A.Brown. 1990. Gene Cloning. Chapman & Hall.
James D. Watson, Michael Gilman, Jan A. Witkowski and Mark Zoller. 1992.
Recombinant DNA Technology. W. H. Freeman.
Page 19 of 73
BIO 206 DOWNSTREAM PROCESSING IN BIOTECHNOLOGY-I

[3 1 0 4]
Role of Downstream Processing in Biotechnology: Role and importance of downstream

processing in biotechnological processes. Problems and requirements of bioproduct
purification. Economics and downstream processing in Biotechnology. Cost cutting
strategies, characterstics of biological mixtures, process design criteria for various classes of
bioproducts (high volume-low value products and low volumehigh value products),
physicochemical basis of bio-separation processes.
[10]
Cell disruption: Physical methods- Osmotic shock, grinding with abrasive solid shear, liquid
shear, chemical methods-alkali reagents, enzymatic methods. Different methods of cell
disruption Advantages & Disadvantages
[06]
Removal of insoluble, biomass (and particulate debris) separation: flocculation and
sedimentation, centrifugation and filtration methods. Designing of centrifuges for desired
product of desired capacity; filtration principles, filter media and equipment, design of
filtration systems
[14]
Membrane based separations (Micro- and Ultra-filtration) theory; design and configuration
of membrane separation equipment; applications; solute polarization and cake formation in
membrane ultra filtration causes, consequences and control techniques; enzyme processing
using ultra filtration membranes; separation by solvent membranes; ultra filtration and
reverse osmosis.
[08]
Precipitation methods with salts, organic solvents, and polymers; colloidal stability of protein
solutions; kinetics of protein aggregation
[04]
Extraction: batch extractions, staged extractions-cross current, co current, counter current
extractions. Differential extractions, fractional extractions with a stationary phase, fractional
extractions with two moving phases.
[06]
Texts/References:
Bioseperations: Principles and Techniques by B.Sivasankar, PHI Learning Pvt.

Ltd., 2006
Bioseparation Downstream processing for biotechnology by Belter P.A.,

Cussler E. and Wei Shan Hu., Wiley Interscience Pub, 1988.
Separation Processes in Biotechnology by Asenjo J. and Dekker M, 1993.
Product Recovery in Bioprocess Technology - BIOTOL Series, VCH, 1990
Unit Operations of Chemical Engineering by McCabe & Smith, McGraw Hill Inc,
, 1993
Separation Process Principles by J. D. Seader & Ernest J. Henley, Wiley, 1998
Page 20 of 73
BIO 208 HEAT TRANSFER OPERATIONS IN BIOPROCESSING

[3 1 0 4]
Importance of heat transfer operations modes of heat transfer
[02]
Conduction: Steady state one dimensional heat flow Fouriers law conduction through
plane wall, cylinder and sphere with constant thermal conductivity variation of thermal
conductivity with temperature conduction through multilayers conduction with uniform
internal heat generation applied to bioprocessing. Biological heat production: Microbial
systems, Human and animal systems. Unsteady state conduction Fouriers second law
application of the solution for various geometries.
[10]
Convection: Natural and forced convection- Forced convection through circular and
noncircular cross sections applied to special cases of bioprocessing. Co-current and
countercurrent types of flow- derivation of LMTD - overall coefficient- determination of film
coefficients- Dimensional analysis - Wilsons method - Analogies.
[12]
Heat transfer with phase change types of condensation Evaporation types of
evaporators Enthalpy balances in single and multiple evaporators economy and capacity
of evaporator.
[12]
Process design of heat exchangers Design procedure of a double pipe and shell and tube (12) exchanger.
[06]
Radiation: Blackbody radiation Kirchhoffs law Stefan Boltzman law view factors
radiation between two large parallel planes radiation with shields.
[06]

Coulson and Richardson. 2001. Chemical Engineering - Vol. I. ELBS
Kern D Q. 1965. Process Heat Transfer. McGraw Hill Inc.
Arthur T Johnson. 1998. Biological Process Engineering. John Wiley & Sons
Page 21 of 73
BIO 210 MOLECULAR BIOLOGY AND GENETIC ENGINEERING LAB [ 0 1 3 2 ]

In the MBG Lab, the following experiments will be performed based on the theory taught
in the CMB course work.
1.
2.
3.
4.
5.
Extraction of Bacterial Genomic DNA

Agarose Gel Electrophoresis
Extraction of Plant Genomic DNA
Extraction of Plasmid DNA
Sodium Dodecylsulphate Polyacrylamide Gel Electrophoresis (SDS-PAGE) of
Proteins
6. Restriction Digestion of Lambda () DNA
7. Ligation Analysis
8. Preparation of Competent Cells
9. Bacterial Transformation
10. Polymerase Chain Reaction
11. Sandwich Dot ELISA Test
References:
David Friefelder. 1987. Molecular Biology. Jones and Bartlett Publishers Inc.
J.M. Walker and E.B. Gingold. 1999. Molecular biology and Biotechnology. Panima
Publishing Corporation.
Page 22 of 73
BIO 212
UNIT OPERATIONS LABORATORY
[0 0 3 1]
In this Lab., the following experiments will be performed based on the theory taught in
Fluid flow and Heat Transfer Operations.
List of Experiments:
1. Orifice meter
2. Venturi Meter
3. Flow through straight pipes
4. Flow through fittings
5. Fluidized Bed
6. Packed bed
7. Bare & Finned tubes
8. Shell & Tube Heat exchange
9. Natural convection
10. Forced convection
Reference:
Page 23 of 73
Open Elective
BIO 316
No of periods: 36
INTRODUCTION TO GENETICS
[3 0 0 3]
Fundamentals of Chromosomes and Inheritance

[06]
Chromosome structure and organisation in prokaryotes and eukaryotes; Continuity of life and
inheritance of chanracters; History of Genetics - Classical experiments of Hershey and
Chase, Avery McLeod etc., Genetic transfer: Conjugation, Transduction and Transformation
Cell Division
[04]
Mitosis; Need for chromosome coiling and uncoiling; Meiosis; Life cycles and chromosomes
Mendels Principle of Segregation
[05]
Mendels Experiments; Mendels principle of Segregation; Phenotype and genotype;
Backcross and testcross; Incomplete Dominance; F2 ratios; Chromosomes and Mendels Law
of Segregation
Mendels Principle of Independent Assortment
[05]
Dihybrid cross; Independent Assortment; Mendels principle of independent assortment;
Dihybrid testcross; Trihybrids and polyhybrids; Chromosomes and the independent
assortment of gene; The chromosome theory of inheritance
Linkage, Crossing Over and Gene Mapping
[06]
Experiments of Bateson and Punnett; Morgans Work; Bridges Experiment; Gene Mapping;
Three-point cross; Double crossing over and gene mapping; Interference; Models of Crossing
Over
Sex chromosomes and sex linked inherited disorders.
[05]
Sex determination by enbvironment; Sex determination by chromosomes; Inheritance related
to sex; Sex-linkage in man.
Multiple alleles and blood group antigens
[05]
Human blood groups; Agglutination; Multiple alleles and the inheritance of blood groups of
ABO series; Precursor antigens; Legal implications of blood group inheritance; Medical
aspects; Secretors; Rh blood antigens; MN Blood Antigen
Text books:
1. Sarin C. Genetics. TMH Publishing Company. 1985.
2. Simmons, Gardner and Snustad. Principles of Genetics. Wiley India. 2006.
References:
1. Griffiths, Anthony J. F., Jeffrey H. Miller, David T. Suzuki, Richard C. Lewontin,
and William M. Gelbart. An Introduction to Genetic Analysis. 7th ed. New York: W.
H. Freeman, 2000.
2. Goodenough U., "Genetics ", Hold Saunders International, 1985.
3. Willliam S. Klug and Michael R. Cummings Concepts of Genetics. 7th ed. Pearson
education, 2004.
Page 24 of 73
B.E- BIOTECHNOLOGY
FIFTH SEMESTER
Page 25 of 73
BIO 301 CHEMICAL AND BIOCHEMICAL ENGG THERMODYNAMICS

[4 0 0 4]
Introduction
[06]
Scope and definition of Thermodynamics, Internal Energy, First law of Thermodynamics,
Energy balance for closed systems, Thermodynamic state and state functions, Equilibrium,
phase rule, reversible processes, Constant volume, constant pressure and isothermal
processes, Enthalpy and heat capacities, Enthalpy of binding, of hydration, Isothermal
titration calorimetry, Differential scanning calorimetry, Food Energy
Volumetric properties of pure fluids
PVT behavior of pure substances, Ideal gas equation, cubic equation of state
[02]
Second law of thermodynamics

[06]
Statements of second law, heat engine, Thermodynamic temperature scale, entropy, Entropy
change of ideal gas, Mathematical statement of second law, Third Law of Thermodynamics,
Second law for biological systems and life
Thermodynamic properties of fluid
[06]
Property relations for homogenous phases, residual properties, Two phase systems, ClausiusClapeyron Equation, Phase rule, Duhems theorem
Solution thermodynamics
[08]
Fundamental property relation, Chemical potential and Phase Equilibria, Partial properties,
Ideal Gas Mixtures, Fugacity and Fugacity Coefficients, Activity and Activity Coefficients,
Property changes of Mixing
Chemical reaction equilibrium
[10]
Reaction Co-ordinate, Application of equilibrium criteria to chemical reactions, Standard
Gibbs Energy change and Equilibrium constant, Relation of equilibrium constant, Effect of
Temperature on Equilibrium constants, Equilibrium conversions for single reactions
Biochemical thermodynamics
[10]
Free energy calculation- Biochemical example (isomerisation of Glucose 6 phosphate to
fructose 6 phosphate), High energy phosphate compounds as energy shuttlers, phosphate
transfer and coupled reactions, Blood and entropy, Carbon monoxide poisoning , Protein
stability, osmosis, dialysis, Diffusion, membrane transport
J.M.Smith, H.C.Van Ness and M.M.Abbott. 2000. Introduction to Chemical

Engineering Thermodynamics. McGraw Hill International Edition
Jeremy L. Berg, John L. Tomoczko, and Lubert Stryer. 2008. Biochemistry.
W.H.Freeman.
Donald T. Haynie. 2001. Biological Thermodynamics. Cambridge University Press.
Page 26 of 73
BIO 303
FERMENTATION ENGINEERING
[3 1 0 4]
Introduction to Fermentation Processes

[03]
Definition of Fermentation, history of Industrial fermentation, the basics of Industrial
fermentation, fermentation products
Media Design and Sterilization
[15]
Medium requirements for fermentation processes- Carbon, nitrogen, minerals, vitamins and
other complex nutrients; oxygen requirements; Medium formulation for optimal growth and
product formation- examples of simple and complex media; Design and usage of various
commercial media for industrial fermentations. Thermal death kinetics of microorganisms;
Batch and continuous heat -Sterilization of Liquid media; Filter sterilization of liquid media,
Design of Sterilization Equipments.
Transport Phenomena in Bioreactors
[15]
Immobilized enzyme/cell kinetics: Shell balance zero & first order reactions, spherical &
flat plate mode, effectiveness factor derivations (internal & external), mass transfer
correlations. Bioprocess considerations in using animal and plant cell cultures. Oxygen
transfer in submerged fermentation processes: OTR, OUR calculations, kLa estimations; role
of aeration and agitation in oxygen transfer.
Kinetics of Microbial Growth and Product Formation
[10]
Phases of cell growth in batch cultures; Simple unstructured kinetic models for microbial
growth; Monod model; Growth of Filamentous Organisms. Growth associated (primary) and
non-growth associated (secondary) product formation kinetics; Leudeking Piret models;
substrate and product inhibition on cell growth and product formation; Introduction to
Structured Models for growth and product formation.
Utilizing genetically engineered organisms
[05]
Guidelines for choosing host vector system, process constraints genetic instability,
considerations in plasmid design to avoid process problems.
Text Book:
Reference Books:

Fogler, H.S. 1986. Elements of Chemical Reaction Engineering. Prentice Hall.
Levenspiel, O. 1972. Chemical Reaction Engineering. John Wiley.
Page 27 of 73
BIO 305
DOWNSTREAM PROCESSING IN BIOTECHNOLOGY-II
[3 1 0 4]

Theories of adsorption Adsorption isotherms, industrial adsorbents, adsorption equipments
for batch and continuous operations (co current and counter current), adsorption in fixed eds.
Leaching: Principle, equilibria, single stage calculation
[10]
Chromatography principles of chromatographic separation gel filtration, reversed phase,
hydrophobic interaction, ion-exchange, expanded bed adsorption, bio affinity and IMAC,
supercritical fluid chromatography. Design and selection of chromatographic matrices;
modes of operation; Design of large-scale chromatographic separation processes
[12]
Electrophoretic separations (all
electrophoresis), Isoelectric focusing
electrophoresis
techniques,
including
capillary
[04]
Crystallization: Factors governing nucleation and crystal growth. Theory of crystallization.

Design of industrial crystallizers: batch and continuous crystallizers.
[06]
Importance of drying in processes-drying characteristics of materials- theory and mechanism
of drying Estimation of drying rate and drying time in batch drying different types of
drying classification of dryers-continuous drying design features, performance of dryersconcepts of freeze drying, spray drying auxiliary equipments required along with drying
plants.
[08]
Emerging technologies for cell recovery, hybrid separation technologies (membrane
chromatography, electro chromatography etc) Aqueous two-phase extraction, reverse micelle
extraction, supercritical fluid extraction, liquid membranes, in-situ product
removal/integrated bioprocessing
[08]
Texts/References:
Bioseperations: Principles and Techniques by B.Sivasankar, PHI Learning Pvt.
Ltd., 2006
Bioseparation Downstream processing for biotechnology by Belter P.A.,

Cussler E. and Wei Shan Hu., Wiley Interscience Pub, 1988.
Separation Processes in Biotechnology by Asenjo J. and Dekker M, 1993.
Product Recovery in Bioprocess Technology - BIOTOL Series, VCH, 1990
Unit Operations of Chemical Engineering by McCabe & Smith, McGraw Hill Inc,
, 1993
Separation Process Principles by J. D. Seader & Ernest J. Henley, Wiley, 1998

Page 28 of 73
BIO 307
COMPUTATIONAL TECHNIQUES IN BIOTECHNOLOGY
Total Number of Periods: 36
[3 0 0 3]
Algorithm Techniques
[06]
Algorithms and complexity of algorithms, Flow charts, pseudocode, recursive algorithms,
interative vs recursive algorithms, sorting and fibonacci problems, fast vs slow algorithms,
algorithms design techniques.
C++ fundamentals: The C++ character set, Identifier & Keywords, Data type with sizes
variables, Declaration statements, C++ program structure.
Flow of control: Statements and blocks, if-else, nested if, switch loops, for, while, do while,
break and continue.
[04]
Operator and expressions: Arithmetic, relational and logical operators, type conversion,
increment and decrement operators, bitwise, assignment operator, and expression,
precedence and order of evaluation.
[04]
Object oriented programming: Basic concept of object oriented and object base
programming, structured programming vs. object oriented programming, benefits of OOP,
C++ and OOP. Classes and objects: Introduction, defining member functions and data
members, making an outside function inline, nesting of member functions, dynamic
allocation of objects, pointer, static member function, constructor and destructor. Operator
overloading and friend function introduction.
[04]
Class inheritance: Defining a class hierarchy, different kind of inheritance, virtual functions,
pure virtual functions and abstract classes, base and derived class constructor.
Application of Oops for solving biotechnology engineering problems.
[04]
Application of mathematical methods in Biotechnology:
Systems of linear equation using Gauss Siedel and Gauss Elimination method-Least square
method, curve fitting and Regression (linear and polynomial)-Newton Raphson MethodEulers and modified Eulers method -2nd and 4th order Runge Kutta Method-Trapezoidal
rule, Simpsons 1/3 rule and Simpsons 3/8 rule-Finite difference method (finite element
volumes). Introduction and working with statistical tools and optimization
[14
]
Text books:
1. Object oriented programming with C++; E. Balaguruswamy, Tata Mc-Graw Hill
Publishers, 1995.
2. C++ programming language; Njarne Stronstrup, 3rd edition, Addison Wesley, 1997.
3. C++, the complete reference; Herbert Schildt, 2nd edition, TMH, 1998.
4. H.M. Deitel and P.J. Deitel, C++, How to program, 3rd edition, Pearson Education,
Asia.
5. Numerical method for Engineers with software and programming application By Steven C Chopra and Reynolds P Canale.
Page 29 of 73
BIO 309
MASS TRANSFER OPERATIONS IN BIOPROCESSING
[3 1 0 4]
Diffusion in fluids-Molecular and eddy diffusion in gases and liquids. Steady state diffusion
under stagnant and equimolal flow conditions. Measurement and calculation of diffusivities.
Diffusion in multicomponenet gaseous mixtures. Diffusion in solids, its application
[10]
Mass transfer concepts, theories-mass transfer under laminar and turbulent flow past solids.
Mass transfer at fluid surfaces. Mass transfer coefficients, concept of JD, NTU and HTU.
Overall mass transfer coefficient and individual coefficients in binary systems, applications
in gas-liquid and liquid liquid systems. Analogies between momentum heat and mass
transfer.
[10]
Principles of gas absorption; Single and Multi component absorption; Absorption with
Chemical Reaction; Design principles of absorbers; Industrial absorbers; HTU, NTU
concepts
[08]
Humidification, dehumidification, air-water cooling system Basic concepts, psychrometric
chart, its importance, construction and use. Methods of humidification and dehumidification,
equipments, design calculation. Air conditioning & cooling tower principles, types,
operation, design calculations.
[06]
Review of VLE Methods of distillation: Batch, flash, steam, vacuum and molecular
distillation. Fractionation of binary systems- design calculations: McCabe Thiele method.
Elements of multi component distillation azeotropic and extractive distillation.
[14]
Textbooks:
Arthur T.Johnson. 1998. Biological Process Engineering. John Wiley & Sons.
R. E. Treybal. 1980. Mass-Transfer Operations. McGraw-Hill.
Page 30 of 73
BIO 311
BIOPROCESS AND BIOREACTION ENGINEERING
[3 1 0 4]
Reaction Kinetics:
Rate equation, elementary, non-elementary reactions and their mechanisms, temperature
dependency of reaction rate. Analysis of experimental batch reactor data- integral and
differential analysis for constant and variable volume system, fitting of data to complex
reaction Mechanisms.
[14]
Ideal Reactors:
Design for homogeneous reaction system-batch, stirred tank and tubular flow reactors,
Multiple reactor system-size comparison, Recycle reactor, Rectors for autocatalytic reactions,
Design of reactors for multiple reactions.
[12]
Bioreactor Design and Analysis:

Batch reactor performance with Monod cell growth kinetics, Chemostat performance
analysis with Monod kinetics, Enzyme catalysis in Chemostat, Chemostats in series, Fedbatch reactor performance.
[10]
Non-ideal reactors:
Residence time distribution studies- pulse and step input signals, RTD for CSTR and PFR,
calculations of conversions for first and second order reactions, tanks in series and dispersion
models.
[12]
Textbooks:
D.G.Rao. 2010. Introduction to Biochemical Engineering. Tata McGraw-Hill.
Octave Levenspiel. 2003. Chemical Reaction Engineering. John Wiley & Sons
Reference Books:
Arthur T.Johnson. 1998. Biological Process Engineering. John Wiley & Sons
Page 31 of 73
BIO 313
DOWNSTREAM PROCESSING LAB
[0031]
In the Downstream processing lab the following experiments will be performed based on
the theory taught in the previous semester
1. Cell disruption
2. Batch filtration
3. Acetone precipitation
4. Isoelectric precipitation
5. Ammonium sulphate precipitation
6. Aqueous two phase systems binodal curve
7. Partitioning of proteins in aqueous two phase systems
8. Ultrafiltration for protein separation
9. Gel filtration chromatography
10. Affinity chromatography
11. Ion exchange chromatography
12. HPLC
Reference:
1. B.Sivasankar. 2006. Bioseparations: Principles and Techniques. PHI Learning Pvt.
Ltd.
2. Belter P.A., Cussler E. and Wei Shan Hu.. 1988. Bioseparation Downstream
processing for biotechnology, Wiley Interscience Pub.
Page 32 of 73
BIO 315
FERMENTATION ENGINEERING LAB
In this lab., the following experiments will be performed.
[0 0 3 1 ]
1. Effect of enzyme concentration on Enzyme Activity

2. Effect of substrate concentration on Enzyme Activity
3. Effect of pH on Enzyme Activity
4. Effect of Temperature on Enzyme Activity
5. Effect of Heat treatment on Enzyme activity
6. Specificity of Enzyme
7. Inhibition Kinetics-I
8. Inhibition Kinetics-II
9. Effect of temperature on Salivary Amylase
10. Estimation of Biomass
11. Yoghurt Fermentation
12. Bread making
13. Wine Fermentation
Reference Books:
Octave Levenspiel. 2003. Chemical Reaction Engineering. John Wiley & Sons
Page 33 of 73
BIO 317 COMPUTATIONAL TECHNIQUES IN BIOTECHNOLOGYLAB [0 0 3 1]

The following exercises will be solved with C++
1
8
9
10
Solution of simultaneous linear algebraic equations:

Gauss-elimination method
Gauss-seidel method
Solution of Non-linear equations:
Bisection method
Successive substitution method
Newton-Raphson method
Statistical analysis of data:
Mean
Standard deviation
Curve Fitting:
Polynomial fit
Exponential fit
Multiregression technique
Interpolation:
Newton forward & central difference method
Langrangian interpolation method
Numerical Integration:
Simpson 1/3 rule
Numerical differentiation:
Langrangian differentiation
Solution of ordinary differential equations: Initial value problems
Solution of ordinary differential equations: Boundary value problems
Finite difference method
Orthogonal collocation method
Solution of partial differential equation: Explicit method
Reference Books:
Pallab Ghosh. 2009. Numerical methods with computer programs in C++. Prentice
Hall.
E. Balaguruswamy. 1995. Object oriented programming with C++. Tata Mc-Graw

Hill Publishers.
Page 34 of 73
B.E BIOTECHNOLOGY
SIXTH SEMESTER
Page 35 of 73
HUM 302
ESSENTIALS OF MANAGEMENT
Definition of Management, its nature and scope, the functions of managers.
[4 0 0 4]
[02]
Planning: Types of plans, steps in planning, process of MBO, How to set objectives,
strategies, policies and planning premises. Strategic planning process: Decision making:
steps in decision making, systems approach.
[09]
Organizing: Nature and purpose of organizing, span of management, factors determining the
span, basic departmentation, Line and staff concepts, functional authority, art of delegation,
decentralization of authority.
[09]
Human Resource Management: Systems approach to staffing, selection process, techniques
and instruments, approaches to manager development and training
[05]
Human factors in managing: Theories of motivation, special motivation techniques.
Leadership -leadership behaviour and styles, Managerial grid.
[09]
Communication: Process, barriers, effective communication techniques.
[03]
Basic control process, critical control points and standards.
[03]
Control Techniques: Budgets, non-budgetary control devices. Overall and preventive

controls: Budget summaries: Profit and loss control, control through ROI, direct control,
preventive control, developing excellent managers.
[05]
International management: Managerial practices in Japan and USA and application of theory.
The nature and purpose of international business and multinational corporations, unifier
global theory of management.
[03]
Text / Reference Books
Harold Koontz, Heinz Weihrich.1990. Essentials of Management. McGraw-Hill
Peter Drucker. 1983. Management, Task and Responsibility. Harper
Peter Drucker. 1993. The Practice of Management. Collins.
Page 36 of 73
BIO 302
BIOINFORMATICS
[3 1 0 4]
Introduction:
Internet Basics, WWW, E-mail, FTP, Central Dogma of Biology. The Digital code of Life.
Information Theory and Biology
[04]
Databases:
a) Sequence Databases: Introduction, Primary and secondary databases, Nucleotide and
protein sequence databases. Format vs. Content, The Gene Bank database, Flat file -A
dissection.
[06]
b) Information retrieval from Biological databases, Retrieving Database Entries: Integrated
Information Retrieval, The SRS & Entrez System, Pattern search from nucleotide and
protein sequences. PROSITE syntax and usage
[04]
c) Structure databases: Introduction to Structures, Protein Data Bank (PDB), Molecular
Modeling Database (MMDB) at NCBI, Structure file formats, visualizing structural
information, structure viewer
[04]
Database Sequence Search & Alignment:
[10]
Introduction, The evolutionary basis of sequence alignment, the Modular Nature of proteins,
Optional Alignment Methods, Substitution scores and Gap penalties, Statistical significance
of Alignments, Database similarity searching, FASTA, BLAST, Low- Complexity Regions,
Repetitive Elements. Practical Aspect of Multiple, Sequence Alignment -Progressive
Alignment Methods, Motifs and Patterns, Presentation Methods.
Phylogenetic Analysis:
[10]
Elements of phylogenetic Models, Phylogenetic Data Analysis: Alignment, Substitution
Model Building, Tree Building, and Tree Evaluation, Building the Data Model (Alignment),
Determining the Substitution Model, Tree -Building Methods, Searching for Trees, Rooting
Trees, Evaluating Trees and Data, Phylogenetic software, Phylogenetics on the web, some
simple practical considerations.
Predictive Methods:
[10]
Predictive Methods using Nucleotide sequences: Framework, Masking repetitive DNA,
Database searches, Codon Bias Detection, Detecting Functional Sites in the DNA, finding
RNA Genes. Detecting ORFs, Detecting Exons and Introns. DNA Microarray, comparative
genomics, genome annotation. Fundamentals of genome compression.
Page 37 of 73
Predictive Methods using Protein sequences: Protein Identity based on composition, Physical
properties Based on sequence, secondary structure and folding classes, specialized structures
or features, tertiary structure. PCR Primer Design: Restriction mapping, Primer design for
PCR Sequencing, Primer design programs and software.
Text Books:
Andreas D Baxevanis. 2004. BIOINFORMATICS A practical Guide to the Analysis

of Genes and Proteins. Wiley Interscience.
David R. Westhead. 2003. Instant Notes: Bioinformatics. BIOS Scientific Publishers

Ltd.
Reference Books:
David W Mount. 2001. BIOINFORMATICS: Sequence and Genome Analysis. Cold

Spring Harbor
Arthur M. Lesk. 2002. Introduction to Bioinformatics. Oxford University Press.
Smith D. W. 1993. Biocomputing Informatics and the Genome Projects. Academic

Press.
Stuart M Brown. 2000. BIOINFORMATICS: A biologists guide to biocomputing and

the internet. NYU Medical Center.
R F Doolittle. 1996. Computational methods for macromolecular sequence analysis.

Academic Press.
S.L.Salzberg, D B Searls, S Kasif (Eds.). 1998. Computational methods in Molecular

Biology. Elsevier.
Page 38 of 73
BIO 304
Total no. of periods: 48
BIOPROCESS CONTROL
[3 1 0 4]
Needs and incentives for Bioprocess control: Design aspects of a bioprocess control system,
Hardware for a bioprocess control system, bioprocess variables measurement and sensor
elements.
[04]
Mathematical modeling of chemical and bioprocesses: General modeling principles system atic
approach for developing dynamic models, Conservation laws, Typical illustrations, degrees of
freedom analysis
[04]
Introduction to Laplace Transforms: Standard forms, General procedure for solving ordinary
differential equations using Laplace transforms
[03]
Development of Transfer fns: Properties of transfer fns, linearizn of nonlinear models.
[02]
Dynamic behavior of first and second order processes: Responses of standard input functions,
Responses to integrating functions, Dynamic characteristics of processes with time delay and
interacting and non interacting systems
[05]
Introduction to feedback controllers: Basic modes of controls - proportional, integral derivative
control, controller combinations, features of PID controllers, on-off controllers
[04]
Final control elements: Control valves pneumatic control valve, specifying and sizing control
valves, valve characteristics, valve positioners.
[03]
Overview of control system design: Steps in control system design control degrees of
freedom selection of controlled, manipulated and measured variables.
[02]
Dynamic behavior and stability of closed loop control systems: Closed loop transfer functions,
Closed loop responses of simple control systems, stability of closed loop control systems,
Routh stability criterion, Root locus diagrams.
[06]
PID controller design: Tuning and trouble shooting, Performance criteria for closed loop
systems, Controller tuning relations, Guidelines for common control loops, Trouble shooting
control loops.
[04]
Page 39 of 73
Frequency response analysis: Sinusoidal forcing of first and second order processes, Bode
diagrams, Nyquist diagrams, Stability criterion, gain and phase margin, Robustness analysis
[06]
Feed forward and ratio control: Characteristics, cascade control, tuning of controllers
[05]
D.E. Seborg, T.F. Edgar and D.A. Mellichamp. 2004. Process Dynamics and control
John Willey & Sons
G. Stephanopoulos. 1983. Chemical Process Control. An Introduction to Theory and

Practice. Prentice Hall International.
J.B. Riggs and M. Nazmul Karim. 2008. Chemical and Bioprocess control. Ferret
Publishers.
Page 40 of 73
BIO 306
BIOPROCESS EQUIPMENT DESIGN
Total No. periods: 48
[3 1 0 4]
Design of Pressure Vessels:

[08]
Proportioning of pressure vessels, selection of L/D ratio, optimum proportions of vessels.
Design of unfired pressure vessels: codes and standards for pressure vessels, types of
pressure vessels, material of construction, selection of corrosion allowance and weld joint
efficiency
Vessels for Biotechnology:
[03]
Design and Materials: Introduction, relevant guidelines, vessel material, surface treatment,
design considerations, components of the biotechnological vessel
Piping and valves for Biotechnology
[03]
Design, piping materials, polishing, sizing of pipes and tubes, connections and cleanability,
valves.
Design of fermentors
[05]
Flow Patterns in Agitated Tanks, Power Requirements for Mixing, Ungassed Newtonian &
Non-Newtonian Fluids, Gassed Fluids, Improving Mixing in Fermenters, Effect of
Rheological Properties on Mixing, Role of Shear in Stirred Fermenters, Scale-up of
fermentors
Design criteria for fermentor, achievement & maintenance of aseptic conditions in
fermentors
[03]
Design of chemostat, design of bubble column fermentor and air lift fermentor
[10]
Heat Exchangers
[08]
Introduction, types of heat exchangers, codes and standards for heat exchangers, materials of
construction, baffles and tie rods, Design of shell and tube heat exchangers (U tube and fixed
tube) as per TEMA standards
Evaporator design
Introduction, types of evaporators, types of feeding, design of triple effect evaporators
[08]

1. Process Equipment Design by M.V. Joshi, McMillan India.
2. Bioprocess Engineering-Systems, Equipment and Facilities Edited by Bjorn K.
Lydersen, Nancy A Delia and Kim L. Nelson. A Wiley Interscience Publication.
3. Principles of Fermentation Technology by PF STANBURY, S. Hall, A. Whitaker, 2nd
Edition, Elsevier Science Publishers, 2003
4. Process equipment design by L.E. Brownell and E. Young, John Wiley, New
York, 1963.
Page 41 of 73
BIO 308
BIOINFORMATICS LAB
[0 0 3 1]
In the Bioinformatics lab the following exercises will be performed based on the theory
taught in the Bioinformatics and CMB course works
1.Sequence Alignment:
Similarity search
Pairwise Alignment
Multiple Alignment
2.Basics of PERL programming:
DNA transcription
mRNA Translation by using Codon Table
mRNA Translation by Open Reading Frame
3. Primer Design
Problem
4.Phylogeny:
Tree construction & Bootstrap
5.Secondary structure prediction

6.Structure Analysis:
Visualization & Analysis
7.Structure Alignment:
Homology & Distant Relationship
8.Protein Modeling:
Homology/Comparative modeling
9.Structure Validation:
PROCHECK,VERIFY3D,WHATCHEK,WHATIF
10.Molecular Dynamics
11.Molecular Docking
Protein-ligand docking; Protein- Protein docking
Reference Books:
David W Mount. 2001. BIOINFORMATICS: Sequence and Genome Analysis. CSHL.
Arthur M. Lesk. 2002. Introduction to Bioinformatics. Oxford University Press.
Page 42 of 73
BIO 310
BIOPROCESS AND BIOREACTION ENGINEERING LAB
[0 0 3 1]
In the Bioprocess and Bioreaction Engineering lab the following experiments will be
performed based on the theory taught in the previous semester
1.
2.
3.
4.
5.
6.
7.
8.
9.
10.
11.
12.
13.
Enzyme immobilization by entrapment method in alginate gel

Deactivation kinetics of invertase enzyme
Studies on mass transfer effects on the performance of enzyme entrapped in
alginate gel
Batch recycle immobilized packed bed bioreactor
Continuous flow immobilized enzyme packed bed bio reactor
Fluidized bed bioreactor (FBR) for cell cultivation
Residence time distribution (RTD) in packed bed reactor with pulse input
Microbial growth curve
Microbial growth kinetics
Studies on growth kinetics of bacterial species in a fed-batch reactor
Effect various carbon sources on submerged growth of bacterial species
Stirred tank bioreactor (STR) with aeration and agitation for cell cultivation
Studies on performance of lab scale fermentor during the submerged growth of
aerobic cultures.
Reference:
Levenspiel, O. 1972. Chemical Reaction Engineering. John Wiley.
Page 43 of 73
BIO 312
BIOPROCESS EQUIPMENT DRAWING
[0 0 3 1]
In this course drawing of all the process equipments that are taught in the subject
Bioprocess Equipment Design will be dealt with.

1. Process Equipment Design by M.V. Joshi, McMillan India.
2. Bioprocess Engineering-Systems, Equipment and Facilities Edited by Bjorn K.
Lydersen, Nancy A Delia and Kim L. Nelson. A Wiley Interscience Publication.
3. Principles of Fermentation Technology by PF STANBURY, S. Hall, A. Whitaker, 2nd
Edition, Elsevier Science Publishers, 2003
4. Process equipment design by L.E. Brownell and E. Young, John Wiley, New
York, 1963.
Page 44 of 73
LIST OF PROGRAM ELCTIVES

Program
Elective code
Subject
BIO 320
Immunotechnology
BIO 322
Food processing
Technology
Bioethics & Biosafety
Bioremediation
BIO 324
BIO 326
Prerequisites
Teaching
Departmrnt
Cell &Molecular Biology

Genetic Engineering
BIO
BIO
-Fermentation Engineering.
BIO
BIO
Page 45 of 73
BIO 320
IMMUNOTECHNOLOGY
(Prerequisites: CMB & Genetic Engineering)
[3 0 0 3]
The Immune System: Introduction - innate and adaptive immunity. Lymphocytes - their
origin and differentiation; antigens - their structure and classification; complement and their
biological functions; types of immune responses; anatomy of immune response.
[05]
Humoral Immunity: B-lymphocytes and their activation; structure and function of
immunoglobulins; immunoglobulin classes and subclasses. Genetic control of antibody
production. monoclonal antibodies and diagnosis. idiotypes and idiotypic antibodies. Major
histocompatibility complex. Blood Typing: AB, O & Rh.
[05]
Cellular Immunology: Thymus derived lymphocytes (T cells) their classification. antigen
presenting cells (APC) -macrophages. dendritic cells. langerhans cells - their origin,
activation and functions; mechanisms of phagocytosis; identification of cell types of immune
system; immunosuppression. Immune tolerance.
[05]
Antigen Antibody interactions: precipitation, agglutination, neutralization. Immunological
and antibody based assays: RIA, ELISA, Chemiluminescence, ELIspot, FACS, western
blotting,
Immuno
fluorescence,
immuno
precipitation,
immuno
diffusion,
immunoelectrophoresis. Immuno Histochemistry and IHC methods. Immuno electron
microscopy.
[08]
Stem cells: Brief mention about stem cells and applications to immunology,
Immunosuppressive drugs. HLA and disease, mechanisms of immunity to tumor antigens.
[04]
Autoimmunity: Auto antibodies in humans, pathogenic mechanisms, experimental models
of auto immune disease, treatment of auto immune disorders.
[04]
Molecular Immunology: Preparation of vaccines, application of rDNA technology to
production of antibodies. Immunotherapy.
[05]
Roitt I. 1991. Essential Immunology. Blackwell Scientific Publications.
Richard Goldsby, Thomas J. Kindt, Barbara A. Osborne. 2006. Kuby Immunology.
WH Freeman.
Benjarnini E. and Leskowitz. 1991. Immunology - A short course. Wiley Liss.
Sambrook J. 1989. Molecular Cloning. Volumes I, II and III. CSHL Press.
Gabriel Virella. Introduction to Medical Immunology, Fourth Edition, Medical
University of South Carolina Charleston, South Carolina.
Page 46 of 73
BIO 322
FOOD PROCESSING TECHNOLOGY
[3 0 0 3]
(Prerequisites: Industrial Microbiology)

Introduction to Food Processing: Biotechnology in relation to the food industry; nutritive
value of food; types of microorganisms associated with food - their sources, types and
behavior
[06]
Food Spoilage & Preservation: Microbial Spoilage of Vegetables, Fruits, Fresh and
Processed Meats, Poultry and Seafood. Spoilage of miscellaneous Foods, Food-borne
illnesses.
[06]
Food Preservation : Rheology of Food Production, Food Preservation Using Irradiation,
Characteristics of Radiations of Interest in Food Preservation., Principles Underlying the
Destruction of Microorganisms by Irradiation, Processing of Foods for Irradiation,
Application of Radiation. Legal Status of Food Irradiation, Effect of Irradiation of Food
constituents; Food Preservation with Low Temperatures, Food Preservation with High
Temperatures, Preservation of Foods by Drying.
[08]
Biotechnology in Food Industry: Characteristics of Food Industry. Food manufacturing &
processing, common additives, bioorganic additives, spoilage, prevention of spoilage, storage
and preservation through biotechnological means, food packaging. Factors influencing food
product development, marketing, and promotional strategies.
[06]
Food Industry: Basal metabolic rate, influences on nutritional status, dietary strategies for
individuals, diet for specific groups, Market Place, ecologically sustainable production, risks
and benefits of biotechnology to food industry.
[04]
Applied Unit Operations in Food Processing: Unit operations applied to the food processing
industry Fluid flow applications, Heat transfer applications, Centrifugation, Filtration,
Extraction, Membrane separations, Evaporation, Distillation, Absorption, Size reduction,
Mixing, Drying, and Crystallization
[06]
Textbook:
Roger, A., Gordon, B. and John, T. 1989. Food Biotechnology. Cambridge University
Press
Reference Books:
James Jay. 1992. Modern food Microbiology. Kluwer Academic Publishers.
W Lindsay. 1988. Biotechnology Challenges for the flavor and food industry.
Elsevier Applied Science.
Earle, R. L. 1983. Unit operations in food processing. Pergamon Press.

Page 47 of 73
BIO 324
BIOETHICS AND BIOSAFETY
[3 0 0 3]
Public acceptance issues for biotechnology: Case studies/experiences from developing and
developed countries. Biotechnology and hunger: Challenges for the Indian Biotechnological
research and industries. The Cartagena protocol on biosafety. Biosafety management: Key to
the environmentally responsible use of biotechnology. Ethical implications of
biotechnological products and techniques. Social and ethical implications of biological
weapons.
[09]
The legal and socioeconomic impacts of biotechnology, public education of the processes of
biotechnology involved in generating new forms of life for informed decision making with
case studies.
[03]
Biosafety regulations and National and International guidelines with regard to rDNA
technology, transgenic science, GM crops, etc. Experimental protocol approvals, levels of
containment. Guidelines for research in transgenic plants. Good manufacturing practice and
Good lab practice (GMO and GLP).
[09]
Environmental aspects of biotech application. Use of genetically modified organisms and
their release in environment. Special procedures for rDNA based product production.
[04]
Intellectual property rights (IPR), WTO-GATT, TRIPS, international conventions patents
and methods of applications of patents. Plant breeders rights. Legal implications,
Biodiversity and farmers rights. Examples of patents in biotechnology. Special application of
patent laws in biotechnology. Licensing and cross licensing Flavr SavrTm- Tomato as model
case and case studies.
[06]
Beneficial applications and development of research, focus to the need of the poor.
Identification of directions for yield, effect in agriculture, aquaculture etc. Ethics and
Biosafety aspects in Bioremediation.
[05]
1. Biotechnologies and development, UNESCO Publications, 1988
2. A Biotechnologies in developing countries present and future, UNESCO Publishers,
1993
3. Intellectual property rights on biotechnology by Singh K. BCIL, New Delhi.
Page 48 of 73
BIO 326
Total No. of Hours: 36
BIOREMEDIATION
[3 0 0 3]
(Prerequisites: Fermentation Engineering)

Course Objectives:
The proposed course is designed to teach students the scientific and engineering principles of
microbiological treatment technologies to clean up contaminated environments and to
generate valuable resources for the human society. The course will include the following
components: (I) fundamentals of environmental microbiology, (2) bioremediation of organic
contaminants and toxic metals, (3) biodegradation of problem environmental contaminants,
and (4) engineering strategies for bioremediation.
[08]
Introduction to Bioremediation:
Introduction-Constraints and priorities of Bioremediation-Current remediation practicesAdvantages and Disadvantages of bioremediation-Factors influencing BioremediationGlobal Application of Bioremediation Technologies-Bioengineering approaches to the
bioremediation of pollutants.
[06]
Microbial ecology and metabolism:
Factors influencing Growth and Biodegradation-Modelling Growth and BiodegradationOxidation and Reduction reactions-Diversity in Metabolic Processes-Metabolism of Organic
Material-Metabolism of Inorganic material-Phototrophic Metabolism- Cometabolism. [05]
Biodegradation of common contaminant compounds:

Biodegradation of Hydrocarbons- Biodegradation of Halogenated Aliphatic compoundsBiodegradation of Halogenated Aromatic compounds.
[03]
Bioremediation processes:
In Situ Treatment: In situ Bioremediation of soil-In situ Bioremediation of AquifersBioventing and Biosparging, Solid Phase Bioremediation: Land Treatment- land farmingprepared beds-soil piles-operating conditions and process control- Composting-Types of
Composting Systems. Slurry-Phase
Bioremediation:
Process
description-Reactor
configuration and modelling-Design considerations-Operating parameters and Process
control-Case study, Vapor phase Bioremediation: Process description- Biological Filtration
Processes for Decontamination of Air Stream-Biofiltration-Biotrickling FiltrationBioscrubbers-Microscale processes; Zero order and First order reaction- Design
considerations-Operating parameters and Process control-Case study.
[06]
Page 49 of 73
Biotreatment of Metals:
Microbial Transformation of Metals-Biological Treatment Technologies for Metals
Remediation-Bioleaching and Biobenificiation-Bioaccumulation- Oxidation/Reduction
Processes-Biological Methylation-Case studies
[06]
Emerging Bioremediation Techniques:
Phytoremediation of organic, metals and inorganic contaminants: PhytoextractionRhizofiltration-Phytostabilization-Biomonitoring- Biomembrane Reactors, Successful and
Unsuccessful Case Studies in Bioremediation Process
[02]
Text Book:
Eweis, Ergas, Chang and Schroeder. 1998. Bioremediation Principles. McGraw-Hill

Series in Water Resources and Environmental Engineering.
Reference Books:
Martin Alexander. 1999. Biodegradation and Bioremediation. Academic press.
John. T. Cookson, Jr. 1995. Bioremediation engineering; design and application. Mc

Graw Hill, Inc.
Page 50 of 73
Open Elective:
BIO 318
INDUSTRIAL BIOTECHNOLOGY
[3 0 0 3]
Introduction to Biomolecules : Structure, properties and classification - carbohydrates, lipids,

proteins and nucleic acids; Prokaryotes & Eukaryotes; Bacterial Taxonomy, Microscopy,
general structural organization of bacteria and other microorganism
[06]
Different forms of DNA and RNA, Organization of DNA in Prokaryotic and Eukaryotic
Chromosomes; replication, transcription and translation, Post-translational Modifications,
DNA Repair, Mutagenesis and Mutations, Basics of Recombinant DNA Technology
[06]
Historical development of bioprocess technology, An overview of traditional and modern
applications of biotechnological processes, role of bioprocess engineer in the biotechnology
industry, outline of an integrated bioprocess and the various (upstream and downstream) unit
operations involved in bioprocesses, generalized process flow sheets.
[06]
Medium requirements for fermentation processes, Carbon, nitrogen, minerals, vitamins and
other complex nutrients, oxygen requirements, medium formulation of optimal growth and
product formation, examples of simple and complex media, design and usage of various
commercial media for industrial fermentations, thermal death kinetics of microorganisms,
batch and continuous heat, sterilization of liquid media, filter sterilization of liquid media &
Air
[06]
An overview of aerobic and anaerobic fermentation processes and their application in the
biotechnology industry, General requirements of fermentation processes, Basic design and
construction of fermentor and ancillaries, Main parameters to be monitored and controlled in
fermentation processes
[06]
Role of Downstream Processing in Biotechnology: Role and importance of downstream
processing in biotechnological processes. Problems and requirements of purification of
bioproducts. Cost cutting strategies, characteristics of biological mixtures, process design
criteria for various classes of bioproducts (high volume-low value products and low volumehigh value products), physicochemical basis of bio-separation processes, RIPP scheme,
Examples Downstream processing of Primary and secondary metabolite
[06]
Text Books:
1. Bailey and Ollis, " Biochemical Engineering Fundamentals ", McGraw Hill (2nd Ed.),
1986.
2. Shule and Kargi, " Bioprocess Engineering ", Prentice Hall, 1992.
Page 51 of 73
BIO 411
SEMINAR
[0 0 0 1 ]
This course is designed for the students to develop skills in searching technical literature,
coordinating it and making a good presentation. Presentation of a good written report is also
part of exercise. The students will give the seminar on a topic assigned to them, on soft skills
or technical topics.
Page 52 of 73
B.E- BIOTECHNOLOGY
SEVENTH SEMESTER
Page 53 of 73
BIO 401
ANIMAL AND PLANT BIOTECHNOLOGY
[4 0 0 4]
Plant Genome Organization

Organization and features of mitochondrial, nuclear and choloroplast genome Structural
features of gene families in plants, Totipotency, Regeneration of plants
[05]
Plant cell cultivation
Biochemistry of major metabolic pathways, Autotrophic and heterotrophic growth, Plant
growth regulators and elicitors, Cell suspension culture development: methodology, kinetics
of growth and product formation, nutrient optimization, Plant products of industrial
importance, Production of secondary metabolites by plant suspension cultures, Hairy root
cultures and their cultivation.
[12]
Techniques in raising transgencies and IPR
Direct and indirect methods: Mechanical, Femptosyringe, electroporation, biolistic,
Chemical: Protoplast, Biological: Agrobacterium mediated plant transformation, chloroplast
transformation, in-planta transformation. Patent, Plant breeders rights, Implementation of
IPR protection, Emerging mechanisms for technology transfer, Enforcement laws and
regulations
[10]
Animal Cell Organization and nutrient requirement
Special features and organization of animal cells, Animal cell metabolism, Animal cell
growth characteristics, Principles of sterile techniques, Regulation and nutritional
requirements for mass cultivation of animal cell cultures.
[06]
Animal cell cultivation
Substrate and product transport through mammalian cell, Animal cell growth kinetics and
shear force. Micro and Macro carrier attached growth, Cell culture in continuous, perfusion
and hollow-fiber reactor.
[06]
Techniques in animal biotechnology and IPR
Hybridioma technology, Live stock improvement, Gene transfer methods in animals,
Transgenic animals, Applications of Cloning and xenotransplantation, Animal cell
preservation, IPR in Animal Biotechnology
[09]
Text Books / /Reference Books:
R.A. Dixon and Gonzales. Plant Cell Culture: A Practical Approach. IRL Press.
K. Lindsey and M.G.K. Jones. 1990. Plant Biotechnology in Agriculture, Prentice
Hall.
BIOTOL Series. 1994. Invitro Cultivation of Plant cell, Butterworth Heinemann Ltd.
BIOTOL Series. 1994. Invitro Cultivation of Animal cell. Butterworth Heinemann
Ltd.
M. M. Ranga. 2007. Animal Biotechnology. Agrobios.
Bhojwani & Rajdhan. Animal and Plant Biotechnology. Elsevier Science Ltd.
Page 54 of 73
BIO 403 PROCESS ENGINEERING ECONOMICS AND OPTIMIZATION [ 3 1 0 4 ]

Total no of periods: 48
Economics Engineers role, decision making Mathematics of cost comparisons
[02]
Time value of money types of interest relationship between nominal and effective rates
series of cash flows annuity gradient and geometric series Principles of evaluation of
bonds concept of continuous interes
[06]
Economics of selection of alternatives Minimum rate of return equivalence Annual
cost, annual worth, present and future worth, rate of return and captalised cost methods
extra investment - mutually exclusive basis replacement economy evaluation of project
payout time, rate of return on original investment, net present value, discounted cash flow
rate of return
[10]
Depreciation and amortization terms associated with depreciation historical methods of
depreciation accelerated and modified accelerated cost recovery systems depreciation
accounting procedures depletion as applied to exhaustible resources taxes and insurances
implications
[08]
Break even analysis Significance and limitations of breakeven charts effect of change of
variables on breakeven breakeven over 100% capacity optimization of single variable and
multivariable optimization analytical techniques production rates for maximisation of
profit and minimization of cost - Application in fluid flow (optimum diameter), heat transfer
(optimum thickness of insulation, optimum flow rates or optimum temperature in a heat
exchanger), optimum cycle time and heat transfer in evaporator, filtration (optimum cycles
and optimum time of filtration step)
[12]
Cost estimation types of cost estimates estimation of equipment costs, cost indexes and
Williams six-tenths rule cash flow diagram estimation of fixed capital investment
Product cost, Book keeping Balance sheet, Pert/CPM techniques economic feasibility
projects plant location and plant layout
[10]
Peters and Timmerhaus. 1980. Plant Design and Economics for Chemical Engineers,
McGraw Hill.
Schweyer. 1955. Process Engineering Economics, McGraw Hill.
F.C. Jelen and Black J.H. 1983. Cost and Optimization Engineering, McGraw Hill.
Taylor G.A. 1980. Managerial and Engineering Economy. D Van Nostand Co.
Page 55 of 73
BIO 405 MODELING AND SIMULATION IN BIOPROCESS ENGINEERING [3 1 0 4]

Needs and uses of Modeling and Simulation: Application examples, limitations,
classification of Models, systematic procedure for building of bioprocess models, stratum of
physico chemical description, principles of formulation of models, Review of fundamental
laws , continuity equations , component continuity equations, energy equations, equation of
Motion, Examples, other constitutive relations and examples.
Exercises involving
formulation of lumped and distributed parameter models in bioprocess engineering.
[15]
Formulation and solutions of steady state and unsteady state lumped parameter in Bioprocess
Engineering : Typical examples such as bioreactors, batch fermentors and semi batch (fed
batch) bioreactors. Mixed cell bioreactors. Solution of linear and non linear algebraic
equations, linear and nonlinear ODEs using computer software. Bioprocess plant simulation
software.
[15]
Formulation and solution of distributed parameter models in bioprocess engineering: Typical
examples solution techniques for partial differential equations, finite difference techniques,
explicit and implicit methods, method of lines.
[10]
Introduction to population balance models in bioprocess engineering: Limitations of common
modeling procedure, Formulation and solutions of population model equations for
bioprocesses, Typical examples.
[05]
Introduction to stochastic models examples in bioprocess engineering. Review of empirical
modeling, statistical terminology used, linear and nonlinear, Regression analysis Examples
in Bioprocess engineering.
[03]
Reference Books:
W. L. Luyben. 1990. Process Modeling, Simulation and control for chemical

engineers. McGraw Hill.
B.W. Bequette. 2003. Process control modeling, Design and Simulation. Prentice
Hall India
Alkis Constantinicles. 2002. Numerical methods for chemical and Bioprocess
engineers with MAT LAB applications. Prentice Hall India
H. Seinfeld and L. Lapidus. 1974. Mathematical methods in chemical engineering V3,
Process modeling, Estimation and identification, Prentice Hall India
Michael A. Bourdrean and Gregory K. McMillan. 2006. New directions in Bioprocess
modeling and control. Maximizing Process Analytical Technology Benefits. ISA.
Page 56 of 73
BIO 407
PROCESS CONTROL LAB
[0 1 3 2]
In the Process control lab the following experiments will be performed based on the theory
taught in the previous semester
List of Experiments:
1. Flow control Trainer
2. Level Control Trainer
3. Multiprocess Trainer
4. Flapper Nozzle System
5. Control Valve Characteristics
6. Temperature Measurement
7. First Order & Second Order System
8. Interacting and Non Interacting System
9. Gauge Calibration
10. Flow Measurement
Reference:
D.E. Seborg, T.F. Edgar and D.A. Mellichamp. 2004. Process Dynamics and control
John Willey & Sons, Inc.
G. Stephanopoulos. 1983. Chemical Process Control. An Introduction to Theory and
Practice. Prentice Hall International.
Page 57 of 73
BIO 409 MODELLING AND SIMULATION IN BIOPROCESS ENGINEERING LAB

[0 1 3 2]
The following exercises will solved using MATLAB
1. Representation of transfer functions and input-output models using Matlab
commands
2. Steady state error ,steady state gain and time domain analysis of continous time LTI
systems
3. Response plots and stability analysis for biorector
4. Introduction to simulink and building a dynamic model for fermentation process
with simulink
5. Analysis of the dynamic behavior of the chemical processes using simulink
6. Performance analysis of fed batch and chemostat reactors solution for set of
differential equations
7. Performance analysis of bioreactor using matlab-use of phase plane analysis
8. Dynamic behavior of feedback controlled processes using simulink
9. Solution for partial differential equations-a case study of solid state fermentation
10. Design of chemostat using grapher user interface (GUI)
Reference:
W. L. Luyben. 1990. Process Modeling, Simulation and control for chemical

engineers. McGraw Hill.
B.W. Bequette. 2003. Process control modeling, Design and Simulation. PHI.
Alkis Constantinicles. 2002. Numerical methods for chemical and Bioprocess

engineers with MATLAB applications. PHI.
Page 58 of 73
LIST OF PROGRAM ELCTIVES

Program
Elective code
Subject
BIO 421
Biological Industrial
waste water Engineering
Protein Engineering
BIO 423
BIO 425
BIO 427
BIO 429
Prerequisites
Advanced Bioprocess
Engineering
Molecular Modelling &
Drug Design
Multiphase Bioreactor
Desigh
BIO
Biochemistry
Cell & Molecular Biology
BIO
Introduction Bioprocess Engg
BIO
Bioinformatics
BIO
BIO 431
Genomics & Proteomics
BIO 433
Biopolymer technology
Bioreaction Engineering
Mass Transfer in
Bioprocessing
Cell & Molecular Biology
Bioinformatics
BIO 435
Metabolic Engineering
Biochemistry
BIO 437
Drugs and
Pharmaceutical
Technology
Solid state Fermentation
Solid waste management
Biofuels Engineering
BIO 439
BIO 441
BIO 443
Teaching
Departmrnt
BIO
BIO
BIO
BIO
Biochemistry
BIO
Fermentation Engineering
---
BIO
BIO
BIO
Page 59 of 73
BIO 421 BIOLOGICAL INDUSTRIAL WASTE WATER ENGINEERING [3 0 0 3]

(Prerequisites: Industrial microbiology)
Introduction:
[04]
Fundamentals of Microorganisms: Microbial flora of soil, growth, ecological adaptations,
interactions among soil microorganisms, biogeochemical role of soil microorganisms. Need
for wastewater treatment, Types of wastewater and sources, Components of wastewater,
Theory of aeration and Oxygen sag curve
Characterization:
[08]
Wastewater sampling, standards, characteristics, Physical characteristics: Definition and
Application, Aggregate organic constituents & estimation (BOD, COD) etc, Chemical
characteristics: Definition and Applications, Biological characteristics, identification,
enumeration, Estimation of flow rates and loadings. Toxicity tests
Physical Unit Operations: (problems based on the below topics to be solved)
[06]
Equalization, Mixing,Sedimentation and accelerated gravity separation, Flotation, Granular
media filtration, Gas transfer (aerators) and mixing
Chemical Unit Processes:
Chemical Precipitation, Adsorption, Disinfection, dechlorination
[04]
Bacterial Metabolism:
[04]
Decomposition of Organic compounds, Basic biology, mass & energy balance (Aerobic
Digestion),Basic biology, mass & energy balance (Anaerobic Digestion),General
consideration for choice- Aerobic vs. Anaerobic
Biological Unit Processes:
[10]
Overview of Biological wastewater treatment, Important microorganisms, Bacterial growth,
Kinetics of growth, Aerobic suspended Growth treatment processes, Aerobic attached
Growth treatment processes, Anaerobic suspended Growth treatment processes, Anaerobic
attached Growth treatment processes, Biological nutrient removal, Pond treatment processes,
Composting and factors affecting, Remediation and bioremediation, Disposal of solids and
bio-solids
References:
Metcalf and Eddy. 1991. Wastewater Engineering - Treatment, Disposal and

Reuse. Tata McGraw Hill Publishing Co. Ltd
C.S.Rao. 1991. Environmental Pollution Control Engg. New Age International
(P) Ltd. Publishers
H.J. Jordening, and J.Winter. 2005. Environmental Biotechnology: Concepts and
Applications. Wiley-VCH Verlag GmbH & Co.
Page 60 of 73
BIO 423
PROTEIN ENGINEERING
Prerequisites: Process Biochemistry, Cell and Molecular Biology
[3 0 0 3]
Structure of Proteins: Primary structure and its determination, secondary structure prediction
and determination super secondary structure, protein folding pathways, tertiary structure and
domain in proteins, quaternary structure, methods to determine tertiary and quaternary
structure, post transnational modification.
[18]
Protein Engineering and Design: Methods of protein isolation, purification and quantitation;
large scale synthesis of proteins, design and synthesis of peptides, use of peptides in biology,
methods of detection and analysis of proteins. Protein database analysis, methods to alter
primary structure of proteins, examples of engineered proteins, protein design, principles and
examples
[18]
Moody P.C.E. and A.J. Wilkinson. 1990. Protein Engineering, ILR Press.
Creighton T.E. 1993. Proteins. Freeman.
Branden C. and Tooze R. 1993. Introduction of Protein Structure. Garland.
Page 61 of 73
BIO 425
ADVANCED BIOPROCESS ENGINEERING
[3 0 0 3]

Prerequisites: Introduction to Bioprocess Engg
Commercial Applications of Microbial Genetics and Mutant Populations:

[04]
Cellular Control Systems Implications for Medium Formulation; Utilization of
Auxotrophic Mutants; Mutants with altered Regulatory Systems.
Animal and Plant Cell Reactor Technology:
[06]
Environmental requirements for Animal Cell Cultivation; Reactors for large-scale production
using animal cells; Plant cell cultivation.
Analysis of Multiple Interacting Microbial Populations:
[10]
Neutralism; Mutualism; Commensalism; Amensalism; Classification of interactions between
two species; Competition Volterras Analysis of Competition, Competition and Selection in
a Chemostat; Predation and Parasitism The Lotka-Volterra Model of Predator-Prey
Oscillations, A multispecies extension of the Lotka-Volterra Model, Other One-PredatorOne-Prey Models; Effects of the number of species and their web of interactions Trophic
levels, Food chains, Food webs, Population Dynamics in models of Mass-Action Form,
Qualitative Stability; Stability of Large, Randomly constructed Food Webs, Bifurcation and
Complicated Dynamics; Spatial Patterns
Mixed Microbial Populations in Applications and Natural Systems:
[10]
Uses of Well-defined Mixed Populations; Spoilage and product manufacture by Spontaneous
Mixed Cultures; Microbial Participation in the Natural Cycles of Matter Overall cycles of
the elements of life, Interrelationships of microorganisms in the soil and other natural
ecosystems; Biological Wastewater Treatment Wastewater characteristics, Activatedsludge process; Design and modeling of activated-sludge processes, Aerobic digestion,
Nitrification, Secondary treatment using a trickling biological filter, Anaerobic digestion,
Mathematical modeling of anaerobic-digester dynamics, Anaerobic denitrification,
Phosphate removal.
Bioprocess Economics:
[06]
Process Economics; Bioproduct Regulation; General Fermentation Process Economics with
an example; Fine Chemicals Proteins from rDNA, Antibiotics, Vitamins, Alkaloids,
Nucleosides, Steroids, Monoclonal Antibodies; Bulk Oxygenates Brewing & Wine
Making, Fuel Alcohol Production, Organic and amino acid manufacture; Single Cell Protein;
Anaerobic Methane Production.
Textbooks:
James E.Bailey and David F.Ollis. 1986. Biochemical Engineering Fundamentals.

McGraw Hill.
Reference books:
Page 62 of 73
BIO 427
MOLECULAR MODELING AND DRUG DESIGN
Prerequisites: Bioinformatics
[3 0 0 3]
Introduction: General concepts of Pharmacology, Biological membranes, Penetration of

membranes by organic molecules, Drug targets, Drug solubility, Drug stability,
Bioavailability, Compartments and clearance, Drug absorption and transport, Drug
metabolism, Pharmacokinetic models, The Blood-Brain Barrier (BBB).
[08]
Drug Structure: Drug definition, Chemoinformatics/Cheminformatics, Conceptual
convergence of biology and chemistry through informatics, Molecular similarity,
dissimilarity and diversity, Chemical Graphs.
[04]
Drug Design: Computational Drug Discovery, Binding interactions, Lipinskis rule of five
(RO5), Lead-likeness, Drug-likeness, Drugability assessment, 1D search Simplified
Molecular Input Line Entry Specification (SMILES), Molecular Descriptors chemical,
topological and geometrical descriptors.
[08]
Molecular Modeling: Molecular Orbital theory. Small molecular modeling, 1D, 2D and 3D
analyses, Computer Simulation Methods: Molecular Dynamics methods, Binding affinity
calculations and conformational analysis. Pharmacophore, Quantitative Structure Activity
Relationships (QSAR), Agonists and Antagonists: Occupancy and Rate theory of Drug
Action, Affinity (Binding) assays, Forces related to Drug Binding and Solvation.
[08]
To Discover and Design New Molecules: Molecular modeling in drug discovery, De novo
ligand design, Similarity search - Virtual screening, Molecular docking Structure Based
methods to identify lead components, QSAR and its applications, Sustained release prodrug
systems, SNPs and Pharmacogenomics, Optimization of reactions, Synthetic considerations,
Combinatorial synthesis, Adverse effects, Toxicology, Clinical trials, Regulatory affairs &
Patenting
[08]
Text Books:
A.R.Leach. 2001. Molecular Modelling Principles and Applications. Longman.

J.M. Haile. 1997. Molecular Dynamics Simulation Elementary Methods. J.Wiley and
Sons.
Reference Books:
Abby L. Parrill. 1999. Rational Drug Design: Novel Methodology and Practical
Applications. American Chemical Society.
U.Madsen. 2002. Textbook of Drug Design and Discovery. Taylor & Francis.
Page 63 of 73
BIO-429
MULTIPHASE BIOREACTOR DESIGN
(Prerequisites:Bioreaction Engineering & Mass Transfer in Bioprocessing)
[3 0 0 3]
New Methodologies for Multiphase Bioreactors : Hydrodynamic and Mass Transfer

Characteristics of Multistage Slurry Reactors , Image Analysis and Multiphase Bioreactors:
image processing, biomass characterization, bioreactor imaging, , Data Acquisition,
Modelling and Control: components in bioprocess monitoring and control, measuring
techniques for pH, pressure, oxygen, carbon dioxide, biomass, classical modeling,basic
modeling concepts, cell models
[06]
Design and Modeling of Immobilized Biocatalytic Reactors: Biocatalyst immobilization
and performance, immobilized enzyme reactors, modeling of immobilized biocatalyst
reactors
[04]
Advances in the Selection and Design of Two-Liquid Phase Biocatalytic Reactors:
classification of two-liquid phase biocatalytic processes, two liquid phase reactors: types of
reactors, mass transfer and reaction kinetics, reactor operation, downstream processing,
process scale-up
[04]
Enzymatic Membrane Reactors: The concept of a membrane bioreactor, enzyme retention,
substrate retention and product separation, membranes and modules, direct contact
membrane reactors, multiphase membrane reactors, applications of enzyme membrane
reactors.
[04]
Reversed Micellar Bioreaction Systems: Reversed micellar principles, determinant factors
for biocatalysis in reversed micelles, reversed micellar bioreactors, characterization of
micellar membrane bioreactors
[06]
Design of Liquid-Liquid-Solid Fluidised-Bed Bioreactors: Process lay-out and operation
for three-phase fluidized bed bioreactor, loop reactors, performance of a conventional
bioreactor and a three-phase fluidized-bed bioreactor
[06]
Bioreactor Design For Plant Cell Suspension Cultures: characterization of plant cells as
biocatalysts: morphology, broth rheology, oxygen requirement, shear sensitivity,
performance in suspension culture, bioreactor design and analysis for plant cells
[06]
Textbooks:
Joaquim M.S.Cabral. 2009. Multiphase Bioreactor design, Taylor & Francis.
Page 64 of 73
BIO 431
GENOMICS AND PROTEOMICS
(Prerequisites: Cell and Molecular Biology, Bioinformatics)
[3 0 0 3]
Introduction: Genes and Proteins, Genomics and proteomics, unicellular genomes, Metazoan
Genomes, Typical human Gene, Evolution of Genomes.
[06]
Sequencing & Genome Projects: Early sequencing efforts. Methods of preparing genomic
DNA for sequencing, DNA sequence analysis methods, Sanger Dideoxy method,
Fluorescence method, shot-gun approach. Genome projects on E.coli., Human genome
project and genetic map.
[06]
Genomics: Raw genome sequence data, expressed sequenced tags (ESTs), Gene variation
and Single Nucleotide Polymorphisms (SNPs), disease association, diagnostic genes and
drug targets, genotyping -DNA Chips, Comparative genomics. Studies with model systems
such as Drosophila, Yeast or C. elegans.
[06]
Proteomics: Methods of protein isolation, purification and quantification. Large scale
synthesis of proteins, use of peptides in biology, analysis of proteins, high throughput
screening, engineering novel proteins. Peptidomimetics, Bioinformatics analysis -clustering
Methods, proteome functional information, two hybrid interaction screens, Mass-spec based
protein expression and post translational modification analysis, Protein Chip interaction
detection.
[08]
Genome Management in Eukaryotes: Multicellularity, cell differentiation and gene
regulation. Inheritance pattern in eukaryotes. Mutations, organization of eukaryotic genome
within the nucleus, eukaryotic transcription units, regulation of transcription, transcription
factors and the co-ordination of gene expression, translation and post-translational
modification in eukaryotes, mitochondrial and chloroplast genome.
[06]
Bioinformatics and Functional Genomics: Bioinformatics Approaches to Gene Expression,
Microarray data analysis, Completed genomes and the Tree of Life, completed Archea,
Bacterial and Viral genomes. Human genome and disease identification, OMIM,
Comparative genomics.
[04]
Textbooks:
John R S Finchman. 1994. Genetic Analysis - Principles, Scope and Objectives.

Blackwell Science.
A.Malcolm Campbell and Laurie J.Heyer. 2006. Discovering Genomics, Proteomics
and Bioinformatics. Pearson.
Reference Books:
Peter M Gresshoff. 1994. Plant Genome Analysis. CRC Press.

Smith D. W. 1994. Biocomputing Informatics and the Genome Projects. Academic
Press.
Benjamin Lewis. 2003. Genes VIII. Oxford University Press
Page 65 of 73
BIO 433
BIOPOLYMER TECHNOLOGY
(Prerequisites: Industrial microbiology)
[3 0 0 3]
Introduction:
Biopolymers The current scenario, different biopolymers produced from various
renewable resources, characteristics, merits and demerits over conventional polymers
[06]
Biopolymer Technology and Applications:
Biopolymers and Artificial Biopolymers in Biomedical Applications, an Overview, Novel
Synthesis of Biopolymers and Their Medical Applications, Composite Films Based on
Poly(Vinylalcohol) and Lignocellulosic Fibres: Preparation and Characterizations,
Composite Materials Based on Gelatin and Fillers from Renewable Resources: Thermal and
Mechanical Properties, Properties of PHAs and Their Correlation to Fermentation Conditions
[08]
Biosynthesis and Modifications:
Synthesis and modification of different Biopolymers like xanthum gum, PHA, PHB etc., [04]
Biosurfactants: Source, characteristics and properties of Biosurfactants; Production of
Biosurfactants via the fermentation and biotransformation routes; Production of
Biosurfactants with immobilized cells; Integrated bioprocess for continuous production of
Biosurfactants including downstream processing; Applications of Biosurfactants Food
Industry, Environmental Control.
[06]
Material Testing and Analytical Methods:
An Overview of Available Testing Methods, Comparison of Test Systems for the
Examination of the Fermentability of Biodegradable Materials, Structure-Biodegradability
Relationship of biopolymers
[06]
Case studies:
Optimization of production and purification of Xanthum gum and other biopolymers like
PHA, PHB
[06]
Reference Books:
1. Emo Chiellini , Emo Chiellini and Helena Gil, Biorelated Polymers: Sustainable
Polymer Science And Technology. Springer 2001
2. R.M. Johnson, L.Y. Mwaikambo and N. Tucker, Biopolymers,Rapra technology
2003
3. Naim Kosaric (Ed). 1993. Biosurfactants. Marcell Dekker Inc.
Page 66 of 73
BIO 435
METABOLIC ENGINEERING
[3 0 0 3]
(Prerequisites: Process Biochemistry)

Introduction: Induction-Jacob Monod model, catabolite regulation, glucose effect, cAMP
deficiency, feed back regulation, regulation in branched pathways, differential regulation by
isoenzymes, concerted feed back regulation, cumulative feedback regulation, amino acid
regulation of RNA synthesis, energy charge, regulation, amino acid regulation of RNA
synthesis, energy charge, regulation, permeability control passive diffusion, active transport
group transportation.
[10]
Synthesis of Primary Metabolites: Alteration of feed back regulation, limiting accumulation
of end products, feedback, resistant mutants, alteration of permeability, metabolites.
[04]
Biosynthesis of Secondary Metabolites: Precursor effects, prophophase, idiophase

relationship, enzyme induction, feedback regulation, catabolite regulation by passing control
of secondary metabolism, producers of secondary metabolites.
[08]
Bioconversions: Advantages of Bioconversions, specificity, yields, factors important to
bioconversion, regulation of enzyme synthesis, mutation, permeability, co-metabolism,
avoidance of product inhibition, mixed or sequential bioconversions, conversion of insoluble
substances.
[08]
Regulation of Enzyme Production: Strain selection, improving fermentation, recognizing
growth cycle peak, induction, feed back repression, catabolite repression, mutants resistant to
repression, gene dosage.
[06]
Textbook:
Wang D.I.C., Cooney C.L., Demain A.L., Dunnil.P., Humphery A.E., Lilly M.D.
1979. Fermentation and Enzyme Technology. John Wiley and Sons.
References:
Stanbury P.F., and Whitaker A. 1999. Principles of Fermentation Technology.

Butterworth-Heinemann.
Zubay G. 1999. Biochemistry. W.C.Brown Publishers.
Page 67 of 73
BIO 437
DRUGS AND PHARMACEUTICAL BIOTECHNOLOGY [3 0 0 3]
(Prerequisites: Process Biochemistry, Industrial Microbiology)
Introduction: Development of drugs and pharmaceutical industry organic therapeutic agents
uses and economics
[04]
Drug Metabolism and Pharmaco-Kinetics: Drug metabolism, physio chemical principles,
radioactivity, pharma kinetics, action of drug on human bodies
[04]
Important Unit Processes and Their Applications: Chemical Conversion processes,
alkylation, carboxylation, condensation and cyclisation; dehydration, esterification,
(alcoholysis), halogenation, oxidation, sulfonation, complex chemical conversions,
fermentation
[08]
Manufacturing Principles: Compressed tablets, wet granulation, dry granulation or slugging,
direct compression, tablet formulation, coating, pills, capsules, sustained action dosage form,
parental, oral liquids, ointments, standard of hygiene and good manufacturing practice [06]
Pharmaceutical products, Analysis and Control: Vitamins cold remedies laxatives
analgesics non steroidal contraceptives external antiseptics antacids and other,
antibiotics biological hormones vitamins - preservation, analytical methods and test for
various drugs and pharmaceuticals, packaging techniques quality control
[06]
Health Biotechnology: Clear air, water and food role of biotechnology, health care
products, edible vaccines, nutrition value of foods, pharming (biopharmaceuticals from
plants), health bioinformatics; microbes and human health, biotechnology kits to monitor day
to day human health
[08]
Heinrich Klefenz. 2002. Industrial pharmaceutical biotechnology. Wiley-VCH.
Susanna Wu-Pong, Yongyut Rojanasakul, and Joseph Robinson.

Biopharmaceutical drug and design and development. Humana Press.
Gary Walsh. 2003. Biopharmaceuticals: Biochemistry and Biotechnology. Wiley Blackwell
Herbert A Kirst and Wu-Kuang Yeh. 2001. Enzyme Technologies for pharmaceutical
and biotechnological applications. Informa Healthcare.
Page 68 of 73
1999.
BIO 439
SOLID STATE FERMENTATION
(Prerequisites: Fermentation Engineering)
[3 0 0 3]
Overview of solid State Fermentation and Bioreactor principles:

The scope of solid State fermentation and its applications, Comparison of solid substrate to
submerged fermentation, general steps of SSF and bioreactor step, the physical structure of
Bioreactor, Macroscopic and microscopic phenomena in Bioreactor operation, the dynamics
of bioreactor.
[05]
Bioreactor types and Selection:
Classification of bioreactors, Design features and operating variables of bioreactor, Criteria
for selection of bioreactor.
[05]
Transport phenomena and the scale up challenges of Bioreactors:
Overall balances of the bioreactor, Mass and Heat transfer phenomena in subsystems
Substrate bed, Head space, Bioreactor wall, Bulk gas flow patterns and pressure drops, Scale
up challenges and approach towards scale up
[06]
Basic Features, Design, Operating Variables of SSF Bioreactors and their Dynamics
Unaerated and Unmixed bioreactors -Trays, Forcefully-Aerated Bioreactors without Mixing
Packed beds, Rotating and stirred drum bioreactors, Continuously mixed and intermittentlymixed bioreactors, Continuous bioreactors Continuous tubular, rotating drum and stirred
tank solid state fermentation bioreactors.
[10]
Fundamentals of Modelling of Bioreactor:
Basic Methodology of modelling of SSF , Kinetic sub model, Modelling of effect of
temperature, water activity on growth, Death kinetics, Modelling of effect of growth on
local environment, substrate, air and thermodynamic parameters involved in modelling,
Modelling of Heat and Mass transfer and the estimation of transfer coefficients.
[10]
Text Books:
D.A.Mitchell, N.Krieger, M.Berovic. 2006. Solid State Fermentation Bioreactors
Fundamentals of Design and Operation. Springer Publication.
Ashok Pandey, Carlos Ricardo Soccol, Christian Larroche. 2007. Current

Developments In Solid-state Fermentation Asiatech Publishers Inc.
Reference:
Development of a model system and a mathematical model for solid-state
fermentation. David Alexander Mitchell, PhD Thesis. University of Queensland
Page 69 of 73
BIO 441
SOLID WASTE MANAGEMENT
[3 0 0 3]
Introduction to solid waste management:

Waste generation in technological society, integrated solid waste management, operation of
solid state waste management systems. Legislative Trends and Impacts: major legislation,
Government agencies.
[03]
Sources, Types and Composition of solid wastes:
Determination of the composition of municipal solid wastes in the field, Physical, chemical
and biological properties of MSW; transformations of solid waste; Properties and
classification of Hazardous wastes; transformations of Hazardous waste constituents found in
MSW; Management of hazardous wastes in MSW.
[08]
Engneering Principles:
Solid waste generation and collection Rates,Collection of solid waste, Separation and
Processing and Transfornmation of solid waste, Transfer and Transport, Disposal of solid
wastes and residual matter: The landfill method, classification and types; Layout and
preliminary design of landfills
[10]
Biological and chemical conversion technologies:
Biological principles, aerobic composting, low and high solids Anaerobic digestion,
development of anaerobic digestion processes and technologies for treatment of the organic
fraction of MSW; other biological transformation processes. Energy production from
biological conversion products, Fermentation and compost processes: Basic processes, feed
stocks, products, design parameters, reactor types, Application and economics, case histories.
[10]
Solid waste management and planning issues: Meeting federal and state mandated
diversion goals:- source reduction and recycling, Implementation of solid waste management
options; planning, siting and permitting of waste management facilities
[05]
Reference books:
1. Integrated solid waste management: Engineering principles and management issues
by George Tchobanoglous, 1993.
2. The solid waste handbook: A practical guide by William D Robinson, P.E., 1986.
Page 70 of 73
BIO 443
BIOFUELS ENGINEERING
[3 0 0 3]
Introduction:
Description of Biofuels; Energy Use & Efficiency; Biofuel Production; Alternative
Energies; Biochemical Pathways Review for Organoheterotrophic, Lithotrophic &
Phototrophic Metabolism; Importance of COD; Biofuel Feedstocks: Starch, Sugar,
Lignocellulosic, Agro & Industrial by-products
[06]
Production of Bioethanol:
Process Technology for Bioethanol production using Sugar; Starch and Lignocellulosic
Feedstocks: Selection of micro-organisms and feedstock; Associated Unit Operations;
Determination of Bioethanol yield; Recovery of Bioethanol; Recent Advances; Process
Integration
[06]
Production of Biodiesel:
Chemical, Thermodynamic & Reaction Kinetic Aspects of Biodiesel Production:
Transesterification and Supercritical Esterification, Saponification and Hydrolysis, Acid &
Base Catalysis; Sources of Oils; Methods of Biodiesel Production General procedure and
Large scale production; Quality Control Aspects.
[06]
Production of Biohydrogen:
Enzymes involved in H2 Production; Photobiological H2 Production: Biophotolysis and
Photofermentation; H2 Production by Fermentation: Biochemical Pathway, Batch
Fermentation, Factors affecting H2 production, Carbon sources, Process and Culture
Parameters; Detection and Quantification of H2 .
[06]
Microbial Fuel Cells:
Biochemical Basis; Fuel Cell Design: Anode & Cathode Compartment, Microbial Cultures,
Redox Mediators, Exchange Membrane, Power Density; MFC Performance Methods:
Substrate & Biomass Measurements, Basic Power Calculations, MFC Performance: Power
Density, Single-Chamber vs Two-Chamber Designs, Wastewater Treatment Effectiveness;
Future Directions.
[06]
Microbial Modeling of Biofuel Production:
Microbial Growth Models: Unstructured, Single Limiting Nutrient Models, Inhibition
Models, Models for Multiple Limiting Substrates, Yield Parameters; Kinetic Rate
Expressions; Bioreactor Operation and Design for Biofuel Production: Batch, CSTR, CSTR
with Cell Recycle, Fed-Batch Systems, Plug Flow Systems; Modeling of Glucose Utilization
and Hydrogen Production; Batch and CSTR Fermentations and Simulations.
[06]
Textbook:
1. Caye M. Drapcho, Nghiem Phu Nhuan and Terry H. Walker. 2008. Biofuels
Engineering Process Technology Mc Graw Hill Publishers, New York.
Reference Books:
1. Jonathan R.Meilenz (Ed.). 2009. Biofuels Methods and Protocols (Methods in
Molecular Biology Series). Humana Press, New York.
2. Lisbeth
Olsson
(Ed.).
2007.
Biofuels
(Advances
in
Biochemical
Engineering/Biotechnology Series). Springer-Verlag Publishers, Berlin.
Page 71 of 73
B.E BIOTECHNOLOGY
EIGHTH SEMESTER
Page 72 of 73
BIO 402
INDUSTRIAL TRAINING
[0 0 0 1 ]
This training, spanning a period of not less than 4 weeks, can be undertaken at the end of
either VI or VII semester level. However, the report must be submitted for the examination at
VIII semester level for evaluation.
BIO 499
PROJECT WORK
[ 0 0 0 20 ]
The students (jointly or individually) at the beginning of 8th semester will be assigned project
work to be carried out in Industry/Institute under the supervision of a guide. The project
should be completed and submitted for evaluation at the end of the semester.
Page 73 of 73

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B.

B.E. BIOTECHNOLOGY (200 Credits)

Introduction to Bioprocess Engineering.

Chemical & Biochemical Engg. Thermodynamics

Cell and Molecular Biology

BIO 320 Immunotechnology

BIO 421 Biological industrial waste water

BIO 429 Multiphase Bioreactor design

BIO 437 Drugs and Pharmaceutical

BIO 322 Food processing Technology

BIO 423 Protein Engineering

BIO 431 Genomics & Proteomics

BIO 439 Solid state fermentation

BIO 324 Bioethics & Biosafety

BIO 425 Advanced Bioprocess Engg

BIO 441 Solid waste management

BIO 326 Bioremediation

BIO 427 Molecular Modeling & Drug design

BIO 435 Metabolic Engineering

BIO 316: Introduction to Genetics

BIO 443 Biofuels Engineering

Erwin Kreyszig. 1985. Advanced Engg. Mathematics.Wiley Eastern.

S.S.Sastry. 1990. Introductory Methods of Numerical Analysis. Prenctice Hall.

B.S.Grewal. Higher Engg.Mathematics, Khanna Publishers

Francis Scheid. 1968. Numerical Analysis. Schaum Series, McGraw Hill.

Pauline M. Doran. 1995. Bioprocess Engineering Principles. Academic Press.

S.Aiba et al. 1973. Biochemical Engineering. Academic Press.

B. Atkinson. 1974. Biochemical Reactors, Pion Ltd.

Albert Lehninger. 1996. Principles of Biochemistry. CBS Publishers.

Lubert Stryer. 2007. Principles of Biochemistry. Freeman.

Voet and Voet. 2005. Biochemistry. Wiley.

Trehan K. 1990. Biochemistry. New Age International.

Introduction: Microbial diversity and taxonomy, Prokaryotes and Eukaryotes, Types of

Pelczar, Chan and Kreig. 1998. Microbiology. W C Brown Pub

Presscot and Dunn. 2004. Industrial Microbiology. CBS Publishers.

Stanier, Ingraham and Wheeler. 1994. General Microbiology. McMillan.

Stoichiometry of microbial growth and product formation, problems based on yield

David M.Himmelblau. 1989. Basic Principles and Calculations in Chemical

Bhat B.I and S.M.Vora, 2005. Stoichiometry. Tata McGraw Hill.

Richard Felder and Ronald W.Rausseau. 1986. Elementary Principles of Chemical

Anderson and Wenzel. 1961. Introduction to Chemical Engineering, McGraw Hill.

Pauline Doran. 1995. Bioprocess Engineering Principles. Academic Press.

BIO 209 FLUID FLOW OPERATIONS IN BIOPROCESSING

Review of fluid statics hydrostatic equilibrium measurement of fluid pressure

Text / Reference Books:

Coulson and Richardson. 2001. Chemical Engineering - Vol. I. ELBS

Badger and Banchero. 1995. Introduction to Chemical engineering. McGraw Hill

Foust. 2008. Principles of Unit Operations. Wiley.

The following experiments will be performed in the Biochemistry Lab.

Albert Lehninger. 1996. Principles of Biochemistry. CBS Publishers.

In the Microbiology Lab, the following experiments will be performed.

Preparation of Liquid and Solid Media.

8. Indole Production Test

Pelczar, Chan and Kreig. 1998. Microbiology. W C Brown Pub

Presscot and Dunn. 2004. Industrial Microbiology. CBS Publishers.

Text / Reference Books:

P .L.Meyer. 1979. Introduction to probability and statistical applications. American

S.S.Sastry. 1990. Introductory Methods of Numerical Analysis, Prentice Hall.

Hogg and Craig. 1975. Introduction of Mathematical Statistics. McMillan

CELL AND MOLECULAR BIOLOGY

DNA Repair, Mutagenesis and Mutations:

Benjamin Lewin. 2003. Genes VII. Oxford University Press.

James D Watson, WH Hopkins, JW Roberts, JA Steitz and AM Weiner. 2008.