THE UNIVERSITY OF THE WEST INDIES EXAMINATION OF _DECEYSER 20° Code and Name of Course: BC 34C - MOLECULAR BIOLOGY 2 Paper: I Date and Time: MONI 9.00 - ate and Tim DAY DECEMBER 16, 2002: 9.00 - 11.00:A.M. Deniion: 2 houre INSTRUCTIONS TO CANDIDATES: This paperhas 1 page(s)and 5 questions ANSWER ANY THREE (3) QUESTIONS ANSWER EACH QUESTION IN A SEPARATE BOOKLET (@) Explain the C-value paradox of eukaryotic genomes (25 marks). (b) Explain the observations of the renaturation kinetics of a typical mammalian DNA. (75 marks) 2. Explain with appropriate illustrations how the incorporation 5-bromouracil and 2- aminopurine during DNA replication can cause modification of the protein encoded by that gene. (100 marks) 3. Describe the process of excision repair. What are the similarities and differences between nucleotide excision repair and base excision repair? (100 marks) 4. Describe the process of conjugation in £. coli. Explain how the DNA, once transferred recombines with the recipient’s chromosome. (100 marks) 5. (@)__Deseribe the mechanism by which enhancers stimulate transcription in ‘eukaryotes. (50 marks), (b) Describe how homothalism contributes to gene expression regulation in yeast. (50 marks)