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Preliminary Study of Lactic Acid Identification from Dahlia Tuber

Waste With Fermentation Process Using Lactobacillus bulgaricus


Elmi Sundari1, Erti Praputri1, Maulana Yusup Rosadi1, Putra Anugrah1
Department of Chemical Engineering, Bung Hatta University
Jl. Gajah Mada 19 Gunung Pangilun Padang
elmisundari@gmail.com

ABSTRACT
The existence of dahlia tuber in Indonesia, especially West Sumatera is very
widely known. One of the benefit of dahlia tuber is inulin production, but the
waste was just wasted away and has not been used optimally. The waste can be
used as raw material for lactic acid production through fermentation processs. The
aim of this research was to study lactic acid production from glucose which is
converted from dahlia tuber by hydrolyis process. The first stage of this research
is to analyze cellulose content out of dahlia tuber. Acid hydrolysis process is then
performed on the samples using 10% sulfuric acid (H 2SO4). After hydrolysis
process, the next step is to analyze glucose content out of hydrolyzate with Luff
Schoorl method then adjusted the samples pH until 4, 5, 6, and 7 by adding 5%
sodium hydroxide (NaOH). The hydrolyzate then fermented using Lactobacillus
bulgaricus lasted for 120 hours at 30C with a speed of 250 rpm. The parametic
measurement included glucose analysis and lactic acid product were done at the
fermentation age of 24, 48, 72, 96 and 120 hours. The results showed that glucose
consumption and lactic acid product were increased following increasing
fermentation age and happened at all of treatment medium. The highest lactic acid
product was 0,4% on medium with pH 4 controlling at 120 hours fermentation.
Lactic acid product on medium with pH 5, 6 dan 7 controlling at 120 hours
fermentation were 0,36%; 0,25%; dan 0,18%.
Keywords: dahlia tuber waste, glucose, Lactobacillus bulgaricus, fermentation,
lactic acid.