Experiment #:

Date:

March 14,2016

Topic:

Genetics

Aim:

To investigate a karyotype.

Introduction:

Humans have 23 pairs of chromosomes: 22 autosomes and a

pair of sex chromosomes. The method by which the
chromosomes are identified and counted is called
karyotyping. Human cells are placed in a solution that
stimulates them to divide by mitosis but the drug
colchicines is added to the solution , which prevents the
formation of spindle microtubules. The chromosomes remain
in metaphase. They are fixed, stained and mounted so that
they can be photographed.

Apparatus:

Scissors, glue, paper

Method:

1. Chromosomes were cut out from diagram and arranged in

pairs and groups as seen in previous diagram.

2. Cut chromosomes were glued to a fresh sheet of paper.

Discussion:

A karyotype is the number and appearance of chromosomes

in the nucleus of a eukaryotic cell. The total number of
chromosomes present from arrangement is forty-six (46).The
individual is observed to be a female as there is the presence
of the sex chromosome X in the karyotype. With no Y
chromosome present and only an X the individual would
have to be female as XX: female and XY: male. A numerical
disorder of the chromosomes is apparent as some
chromosomes are missing and some are in excess
showcasing the condition to be aneuploidy and the
individual to be diagnosed with Turner Syndrome due to
presence of single X chromosome.

Conclusion:

The karyotype investigated presented the aneuploidy

condition of chromosomes, showed that the person was
female and has Turner Syndrome.