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g/L
40
4
1.5
5.2
-
0.95
.vs
0.67
P 2 .N .D 3
Pg = 1.11 o 0.56 i
Q
0.45
Richards correlation:
0.4
Po , [ Hp]
KLa = hr-1
VL = liquid volume, m3
vs = superficial velocity of air based on bioreactor cross-section, m/hr
Pg = power consumed for agitation in gassed system, W
Po = power consumed for agitation in ungassed system, W
N = impeller speed, rps
Q = volumetric of air working volume per minute (VVM)
Di = impeller diameter, m
GIVEN:
VR = 80 m3
VL = 40 m3
T = 32oC
D = 0.40 hr-1
Oxygen-enriched air (31% oxygen) at 1.05 atm
Q = 0.75 VVM
Pg/VL = 1,100 W/m3
YX/O2 = 1.30 g dcw/g O2
YX/S = 0.60 g dcw/g glucose
REQUIRED:
a) Xmax at steady-state
b) OTR if Tin = 30oC and Tout = 34oC
c) !
SOLUTION:
a) The growth of the cell can be limited by (1) oxygen-transfer limitation or (2) availability of necessary nutrients.
Thus, the approach is to evaluate the cell densities, X, that can be sustained by the two aforementioned factors,
respectively, and by comparing the results, we select the maximum possible cell concentration for the given
conditions.
"#$ = "&$
12
'() (+,(
+,( ) =
34/67
Assume standard reactor:
Dt = HL
40 : ; =
<
=
>? 7 (>? )
>? = 3.7067 :
G.HI
0.75
: KLM
40 : ; N. O.
;
: N. O. :LP
R
3.7067 7 : 7
4
60
:LP
M
G.ST
= 525.56 M UV
W=
X67
1 KY:
KY: [
= 0.02761
=
:Z "
+,( 36.22
:Z "7
7
(@ 30] +, ^_M` "7 )
[
+,(
=
X67
0.31 1.05 KY:
:Z "7
=
= 11.7892
KY: [
W
[
0.02761
:Z "7
525. 56 M UV
11.7892
:Z"7
0 =
[
0.40 2
:Z cdN
1.30
:Z "7
e = fg. hi j klm/n maximum cell concentration achievable based on the given aeration condition.
40
2=
Z
[
6 12
1 180
0.50
= if
j klm
pqrsk tu lqvptu qwqxyqpxyxz{
n
4
2=
Z
[
2 14
1 80
0.15
5.2
2=
Z
[
1 31
1 136
0.03
= |. ii
= i|. }h
j klm
pqrsk tu uxzvtjsu qwqxyqpxyxz{
n
j klm
pqrsk tu ~tr~tvtr qwqxyqpxyxz{
n
1.5
2=
Z
[
1 32
1 142
0.01
= ii. g
j klm
pqrsk tu ryv qwqxyqpxyxz{
n
By comparing the results, the maximum cell concentration that can be achieved is limited by the availability of
the nitrogen source. ANSWER: 9.33 g dcw/L.
b)
at 30oC:
X67
1 KY:
KY: [
W= =
= 0.02695
+,( 37.1 :Z "7
:Z "7
[
X67
0.31 1.05 KY:
:Z "7
+,(
=
=
= 12.0779
KY: [
W
[
0.02695
:Z "7
at 34oC:
X67
1 KY:
KY: [
W= =
= 0.02830
+,( 35.34 :Z "7
:Z "7
[
X67
0.31 1.05 KY:
:Z "7
+,( ]?
=
=
= 11.5018
KY: [
W
[
0.02830
:Z "7
+,(
+,(
+,(
+,(
ln
+,(
+,( ]??
+,(
+,( ?
"#$ = 525.56 M UV
11.7875
11.5018 0 (12.0779 0)
= 11.7875
11.5018 0
ln
12.0779 0
:Z"7
jf
= h|}. g
[
n v
c)
1100
:;
40 : ; = 1.11
XG
3.7067
3
0.75 G.IS
2
; G.=I
XG = 61,057.06 = 81.88 X
81.88
40
G.=G
G.IG
0.75 40 60
R
3.7067 7
4
= 7.63 W + 2.37
W = 1.9438 :
! =
O_:` KLM
W
1.9438 :
=
=
= g. ig
O_:` KLM + O_:` L_Lc
W + W( 1.9438 : + 3.7067 :