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Fish extract samples

Date
No. of samples
27/10/2011
13
8/11/2011
16
5/12/2011
17
9/1/2012
17
10/1/2012
17
total

80

Microcystins samples

DNA Samples

Date
No. of samples
27/10/11
11
8/11/2011
10
5/12/2011
10
9/1/2012
10
10/1/2011
10

No of samples

total

51

10
10
10
10
40

Time
FISH SAMPLES 1 WEEK
FILTER SAMPLES 3 DAYS
SAMPLE ANALYSI 1 WEEK
phyto counting 1 WEEK
Microcystis
1 WEEK
Total
extract filter sam3 DAYS
Total

days
7
3
7
7
7
31
3
34

water samples for counting


samples
net+surface+scum&integrated
net+surface+scum&integrated
net+surface+scum&integrated
net+surface+scum&integrated
net+surface+scum&integrated

no.
4
4
4
4
4

Microcystis
4
4
4
4
4

20

20

Analysis
Map with sampling points
table sampling details (date, GPS, weather, )
physico chemical data (mean +/- SD), temp, oxy, Cond, pH
table experimental design
fish species composition (circle diagram or stacked bar)
fish length (histogram, average, min, max)
fish weight (-"'-)
Graphs with ELISA results, MC per g dry weight along time axis for each date separately absolutely
Graphs with ELISA results, MC per g dry weight along time axis for each date separately in percent
show your best and your worst standard curve
graphs with ELISA results for water samples, both absolutely and in proportion of integrated water
graphs with Chl. A results (- " -)

ch date separately absolutely


ch date separately in percentage of control weight

oportion of integrated water samples

Fish composition in Kisumu bay on 27/10/11

%
total fish pieces

75

Barbus sp

25

33.33333

Haplochromis sp

2.666667

Nile perch(lates niloticus)

42
75

56

Rastineobola argentea

Barbus sp

33.30%

Haplochromis sp

2.70%

Nile perch(lates nilotic

8%

Rastineobola argentea

56%

8/11/2011
fish species
Barbus sp

sum

propotion(%)
17 10.49383

10.50%

2 1.234568

1.20%

Nile perch(lates niloticu

46 28.39506

28.40%

Rastineobola argentea

97 59.87654

59.90%

Haplochromis sp

total

5/12/2011

162

59.90%

sum

%
3 2.521008

2.50%

Nile perch(lates niloticu

45 37.81513

37.80%

Rastineobola argentea

71 59.66387

59.70%

Barbus sp
Haplochromis sp

total

59

119

9/1/2012 Rusinga fish samples


sum
%
Barbus sp
1 0.757576

0.76%

Haplochromis sp
Nile perch(lates niloticu

6 4.545455

5%

Rastineobola argentea

127 96.21212

96.20%

total

134
44.66667

10/1/2012 Kisumu bay


sum
Barbus sp

%
19 13.57143

13.60%

18 12.85714

12.90%

Rastineobola argentea

103 73.57143

73.60%

total

140

Haplochromis sp
Nile perch(lates niloticu

73.6

73.6

33.30%

Barbu

2.70%

33.30%

8%
56%

56%

2.70%
8%

Rastineobola
argentea

Lates niloticus
Ni

10.50%
1.20%
59.90%

28.40%

1
2
3
4
5
6
7

2.50%

1
2

37.80%

59.70%

4
5
6
7

0.76% 5%

1
2
3
4
5

96.20%

6
7

13.60%
12.90%

1
2
3
4

73.60%

5
6
7

73.60%

5
6
7

Kisumu bay

fish composition
Barbus sp
Haplochromis sp
Nile perch
Rastineobola

Barbus sp

33.30%
2.70%
8%
Lates niloticus
Nile tilapia

Average
SD
16
2
12
78.25

9.3094933625
0
19.9562020435
27.8732249061

1
2
3
4
5
6
7

Sampling Site

Sampling date

Sampling location Coordinates of sampling points


Latitude

Longitude

Surface, Integrated
Nyanza gulf (Kisu 27.10.11

Patch and Shore

00 10' 26.7'' S 034 44' 11.7'' E

08.11.11

,,

00 09' 20.7'' S 034 43' 45.1'' E

05.12.11

,,

00 09' 20.7'' S 034 43' 45.1'' E

10.01.12

,,

00 10' 26.7'' S 034 44' 11.7'' E

Main lake basin (R 09.01.12

,,

00 22' 46.2'' S 034 11' 15.0'' E

Sampling Site

Sampling date

Sampling locations Coordinates of sampling points


Latitude

Longitude

Surface, Integrated,
Nyanza gulf (Kisumu 27.10.11

Patch and Shore

00 10' 26.7'' S 034 44' 11.7'' E

08.11.11

,,

00 09' 20.7'' S 034 43' 45.1'' E

05.12.11

,,

00 09' 20.7'' S 034 43' 45.1'' E

10.01.12

,,

00 10' 26.7'' S 034 44' 11.7'' E

Main lake basin (Rus 09.01.12

,,

00 22' 46.2'' S 034 11' 15.0'' E

Elevations (m.a.s.l)

Mean water te Mean secchi dept Mean Oxygen


Ph

ngitude

1127

25

26

5.5

6.5

43' 45.1'' E

25

16

6.1

6.3

43' 45.1'' E

25

27

5.8

6.5

44' 11.7'' E

26

6.1

6.5

26

85

7.3

6.5

1134

Elevations (m.a.s.l)

Mean water tempMean secchi depth ( Mean Oxygen Ph

ude

1127

25

26

5.5

6.5

45.1'' E

25

16

6.1

6.3

45.1'' E

25

27

5.8

6.5

26

6.1

6.5

26

85

7.3

6.5

11.7'' E
1134

27/10/11
Fish species
min. wet w Max wet weAverage wet weight(g)%wt
0.1
2.6
1.2
30 42.44855

Barbus sp
Haplochromis sp

0.3

0.4

0.35

0.7 0.990466

Nile perch(lates niloticu

0.2

1.9

0.7333

4.3998 6.225504

Rastineobola argentea

0.3

1.7

0.847

35.574 50.33549
70.6738

Kisumu bay
fish specie average
Barbus sp
Haplochromi

SD

1.089825 0.234558
0.6 0.353553

Nile perch(la 0.639075 0.145911


2.6
Rastineobol

0.655 0.164909

8/11/2011
Fish species
Barbus sp

min. wet w Max wet weAverage wet weight(g)% wt


0.4
2.4
0.994
16.898 15.94331

Haplochromis sp

0.5

1.2

0.85

1.7 1.603955

Nile perch(lates niloticu

0.1

4.2

0.74

34.04 32.11684

Rastineobola argentea

0.1

1.6

0.55

53.35 50.33589
105.988

5/12/2011
Fish species
Barbus sp
Haplochromis sp

min. wet w Max wet weAverage wet weight(g)%wt


1.1
2
1.4333
4.2999
5.66157
0

Nile perch(lates niloticu

0.1

4.6

0.655

29.475 38.80899

Rastineobola argentea

0.1

1.2

0.594

42.174 55.52944
75.9489

9/1/2012
Rusinga Fish Samples
Rusinga
Fish species
min. wet w Max wet weAverage weSTD
Barbus sp
1.2
1.2
1.2
Haplochromis sp

%wt
1.2 1.673874

Nile perch(lates niloticu

1.3

2.3

1.8333 0.463321

10.8 15.06486

Rastineobola argentea

0.1

1.5

0.469 0.213018

59.69 83.26126
71.69
1.2

10/1/2012 Kisumu bay fish samples


Fish species
Barbus sp

min. wet w Max wet weAverage wet weight(g)%wt


0.3
1.9
0.732
13.908 20.06897

Haplochromis sp
Nile perch

0.1

1.9

0.428

Rastineobola argentea

0.1

1.3

0.463

21.0306

21

Haplochromis sp

1.297211

1.3

Nile perch

22.06701

22

Rastineobola argentea

56.25378
100.6486

56
100.3

Kisumu bay
Fish species
Barbus sp

%wt

7.704 11.11672
47.689
69.301

68.8143

barbus
1.6
2.6
1.5
1.4
2.2
1.9
1.2
1.7
1.2
0.9
1.6
0.6
2.3
0.8
1.9
1.3
1.2
0.8
1.1
1.9
0.3
0.3
0.4
0.2
0.1
0.1
1.196154

Haplochromis
0.3
0.4
0.35

Nile perch
1.1
1.9
0.7
0.3
0.2
0.2
0.733333

barbus
2.4
1.1
1.6
1.1
1.2
0.9
0.9
0.9
1.2
0.8
0.7
0.8
0.8
0.7
0.7
0.4
0.7
0.994118

Haplochromis
1.2
0.5
0.85

Nile perch
3.4
4.2
1.4
1.5
1.1
0.6
0.6
0.8
1.1
1.4
0.7
0.6
1
1.5
1.2
1.5
1
1.1
1.1
0.5
0.4
0.5
0.5
0.4
0.4
0.4
0.6
0.6
0.4
0.4
0.4
0.5
0.4
0.3
0.4
0.2
0.3
0.5
0.3
0.3
0.2

Rastineobola
1.3
1.1
1.1
1.2
1.6
1
0.5
0.9
0.8
0.6
0.9
0.6
1.1
1.1
1.3
1.2
0.7
0.9
1.4
1.4
1.2
1.3
0.8
1
0.8
1.1
1
0.8
0.9
1
1
1.3
1.1
0.8
0.7
0.7
0.9
0.9
0.7
0.8
1

0.6
0.3
0.3
0.3
0.4
0.3
0.3
0.2
0.2
0.1
0.739216

0.5
0.4
0.4
0.9
0.5
1.4
1
0.4
0.7
0.1
0.3
0.2
0.3
0.2
0.2
0.1
0.3
0.2
0.2
0.3
0.2
0.2
0.1
0.1
0.1
0.1
0.1
0.2
0.1
0.1
0.1
0.1
0.2
0.1
0.1
0.1
0.3
0.1
0.1
0.1
0.1
0.1
0.1
0.1
0.1

0.1
0.1
0.1
0.1
0.1
0.1
0.1
0.1
0.1
0.1
0.1
0.549485

barbus

Haplochromis
1.2
2
1.1

1.433333

Nile perch
4.6
1.1
0.8
0.4
0.7
0.7
1.3
1.1
2.5
2.1
0.8
0.7
0.3
2
0.7
0.4
1.2
0.7
1.1
0.8
1.2
0.1
0.4
0.2
0.2
0.2
0.2
0.2
0.2

0.2
0.1
0.2
0.1
0.1
0.1
0.2
0.2
0.1
0.1
0.1
0.1
0.1
0.1
0.1
0.654545

barbus

Haplochromis
1.2

Nile perch
1.3
2.2
2.3
2.2
1.7
1.3
1.833333

barbus

Haplochromis
1.1
0.8
0.7
0.7
0.6
0.8
1.1
1.9
0.6
0.7
0.5
0.5
0.5
0.6
1
0.6
0.5
0.4
0.3

Nile perch
1.9
0.6
1
0.5
0.5
0.7
0.3
0.3
0.2
0.3
0.1
0.2
0.2
0.3
0.3
0.1
0.1
0.1
0.427778

0.731579

Rastineobola
0.9
1
1
0.9
1
1.1
0.7
0.9
0.8
0.7
0.7
0.9
0.8
1
0.6
0.6
0.7
0.7
0.5
0.5
0.7
0.3
0.6
0.5
0.6
0.7
0.8
1.3
0.6
0.8
0.5
0.5
0.3
0.9
1

0.8
0.4
0.6
0.6
0.3
0.4
0.5
0.4
0.5
0.2
0.3
0.7
0.4
0.5
0.7
0.6
0.5
0.3
0.6
0.4
0.5
0.5
0.3
0.3
0.5
0.2
0.2
0.4
0.4
0.3
0.5
0.3
0.3
0.2
0.3
0.5
0.3
0.2
0.2
0.2
0.5
0.3
0.2
0.3
0.3

0.2
0.3
0.3
0.2
0.3
0.2
0.2
0.2
0.2
0.2
0.2
0.2
0.1
0.2
0.2
0.2
0.1
0.2
0.2
0.1
0.2
0.3
0.2
0.2
0.1
0.1

0.463208

Wet weight(g)

Rastineobola
0.6
1.1
1.5
1.2
0.8
1.7
1.1
0.7
0.8
1.4
1.3
1.4
1.1
0.9
0.7
0.5
0.4
1.2
0.9
1
0.7
0.8
0.8
0.7
0.9
1
0.9
0.7
1.3
0.6
0.8
0.6
0.4
1.1
0.4
0.4
0.6
1.2
0.8
0.3
0.5
0.3
0.3

3
2.5
2
1.5
1
0.5
0
Barbus sp

Hapl

0.846512

astineobola

Wet weight (g)

4.5
4
3.5
3
2.5
2
1.5
1
0.5
0
Barbus sp

Haplochromis sp
Fish species

Nile perch(lates niloticu

Wet weight (g)

Rastineobola
0.8
0.8
1.1
0.5
1
0.7
0.9
0.9
0.7
0.9
0.7
0.8
1
1.2
0.4
0.5
0.9
0.6
0.8
0.8
0.8
0.9
1.1
0.8
0.7
0.6
0.6
0.6
0.7

5
4.5
4
3.5
3
2.5
2
1.5
1
0.5
0
Barbus sp

Haplochromis

Fish

0.8
0.5
0.9
0.7
0.4
0.4
0.6
0.8
0.6
0.7
0.5
0.4
0.7
0.8
0.8
0.9
0.6
0.5
0.5
0.7
0.4
0.4
0.3
0.5
0.6
0.3
0.1
0.2
0.3
0.6
0.3
0.3
0.1
0.3
0.4
0.3
0.3
0.4
0.2
0.1
0.1
0.1

weight (g)

0.594366
2.5

wet weight (g)

Rastineobola
0.6
0.9
0.9
0.6
0.4
0.7
0.9
0.5
0.5
0.8
0.9
0.9
0.6
0.4
0.8
0.5
0.6
0.8
0.6
0.4
1.5
0.6
0.8
0.8
0.7
0.6
0.2
0.4
0.5
0.4
0.4
0.5
0.7
0.4
0.4
0.4
0.8
0.5
0.7
0.6
0.3

2.5

1.5

0.5

0
Barbus sp

Haplochromis sp

Fish specie

0.4
0.5
0.6
0.7
0.1
0.4
0.6
0.6
0.3
0.4
0.4
0.7
0.1
0.5
0.2
0.6
0.6
0.5
0.5
0.7
0.4
0.2
0.6
0.3
0.5
0.5
0.8
0.4
0.7
0.3
0.1
0.4
0.4
0.6
0.4
0.5
0.5
0.5
0.5
0.6
0.1
0.7
0.5
0.6
0.5

0.5
0.4
0.4
0.4
0.4
0.4
0.4
0.6
0.5
0.2
0.5
0.4
0.4
0.4
0.2
0.4
0.4
0.2
0.2
0.5
0.6
0.3
0.4
0.4
0.3
0.3
0.3
0.3
0.4
0.2
0.6
0.5
0.2
0.5
0.4
0.5
0.3
0.5
0.3
0.5
0.4
0.2
0.3
0.3
0.2

0.2
0.2
0.1
0.1
0.1
0.1

wet weight (g)

0.469343

2
1.8
1.6
1.4
1.2
1
0.8
0.6
0.4
0.2
0
Barbus sp

Haplochromis sp
Fish species

Nile perch

min. wet weigh


Max wet weight
Average wet
weight(g)

Haplochromis sp
Fish Species

Nile perch(lates niloticus)

Rastineobola
argentea

min. wet weigh


Max wet weight
Average wet
weight(g)

Nile perch(lates niloticus)

Rastineobola
argentea

min. wet weigh


Max wet weight
Average wet
weight(g)

Haplochromis sp
Fish species

Nile perch(lates niloticus)

Rastineobola
argentea

min. wet weigh


Max wet weight
Average wet
weight(g)

Haplochromis sp
Fish species

Nile perch(lates niloticus)

Rastineobola
argentea

min. wet weigh


Max wet weight
Average wet
weight(g)

Nile perch

Rastineobola
argentea

min. wet weigh


Max wet weight
Average wet
weight(g)

min. wet weigh


Max wet weight
Average wet
weight(g)

27/10/11
Fish species
min. lengthMax length Average length(mm)
19
64
46.85

Barbus sp
Haplochromis sp

26

30

28

Nile perch(lates niloticus

21

53

35.17

Rastineobola argentea

31

56

44.28

kisumu bay
fish specie average l stdev
Barbus sp
Haplochromi

47.80066 6.542533
31.75 5.303301

Nile perch(lates
34.07833
niloticus 2.803147
Rastineobol

40.0252 4.585002

8/11/2011
Fish species
Barbus sp

min. lengthMax length Average length(mm)


38
65
48.3

Haplochromis sp

31

40

35.5

Nile perch(lates niloticu

12

70

36.96

Rastineobola argentea

10

58

36.16

5/12/2011
Fish species
Barbus sp
Haplochromis sp

min. lengthMax length Average length(mm)


51
63
56
0

Nile perch(lates niloticu

17

70

33.85

Rastineobola argentea

20

56

43.7

9/1/2012
Fish species
Barbus sp

min. lengthMax length Average le stdev


56
56
56

Haplochromis sp
Nile perch(lates niloticu

50

61

57.67 4.320494

Rastineobola argentea

27

56

40.3 5.323458

Rusinga
Average toSTDEV
Fish species
Barbus sp
Haplochromis sp
Nile perch(lates niloticu
Rastineobola argentea

56

57.67

4.3

40.3

5.3

10/1/2012
Fish species
Barbus sp
Haplochromis sp

min. lengthMax length Average length(mm)


29
60 40.05263

Nile perch(lates niloticu


Rastineobola argentea

17

62 30.33333

60 35.96078

barbus
59
64
50
53
60
58
46
52
51
46
55
39
61
44
60
51
52
46
51
60
24
30
41
25
21
19
46.84615

Haplochromis
26
30
28

Nile perch
42
53
35
31
29
21
35.16667

Rastineobola
40
50
52
53
46
56
51
43
42
51
54
41
50
45
40
40
40
51
46
46
43
43
46
40
48
48
50
48
51
36
43
39
35
49
40
38
44
53
46
30
34
32
31

44.27907

barbus
65
51
56
52
53
50
49
50
50
44
42
45
40
45
45
38
46
48.29412

Haplochromis
40
31
35.5

Nile perch
70
50
54
49
39
34
40
51
54
38
36
51
53
52
55
51
50
50
34
35
37
36
32
32
29
36
35
32
30
29
35
32
26
29
26
30
41
33
30
26
32
22
26

27
32
27
32
28
28
12
36.96

barbus

Haplochromis
54
63
51
56

Nile perch
70
47
42
34
39
42
50
50
61
59
44
39
30
59
40
34
50
39
49
24
26
26
26
26
24
21
24
25
21
25
25
25
20
19
20
21
19

21
17
21
33.85

barbus

Haplochromis
56

Nile perch
50
60
60
60
61
55
57.66667

barbus

Haplochromis
53
40
45
53

Nile perch
62
40
44
32

40
40
46
60
35
35
34
32
39
34
39
43
29
30
34

30
33
30
25
25
24
20
20
25
27
29
43
20
17
30.33333

40.05263

Total length(mm)

astineobola

70
60
50
40
30
20
10
0
Barbus sp

Haplochrom

total length (mm)

70

Rastineobola
56
55
45
57
57
53
38
52
51
43
50
44
52
55
54
54
45
52
58
53
55
50
50
50
52
50
46
52
52
52
54
54
51
48
47
49
46
43
48
51
42
42
41

60
50
40
30
20
10
0
Barbus sp

Haplochromis sp

fish s

52
41
55
51
44
48
24
45
25
28
27
24
22
31
23
27
36
25
14
10
10
11
10
12
25
24
21
20
23
35
23
20
21
42
18
20
19
20
26
20
19
21
20
23
20
20
20
20

15
20
19
16
17

Rastineobola
50
50
53
43
50
49
52
54
47
52
47
49
52
56
35
44
50
42
50
49
50
50
54
49
46
43
48
44
43
43
45
52
47
45
44
45
51

total length (mm)

36.15625

70
60
50
40
30
20
10
0
Barbus sp

Haplochromis sp
Fish species

Nile pe

45
48
45
39
49
48
48
49
44
41
42
48
41
42
33
42
44
34
25
35
35
49
48
39
33
39
36
35
41
36
29
22
26
20

Rastineobola
45
42
50
45
46
40
39
48

total length (mm)

43.70423

70
60
50
40
30

total lengt

70
60

42
38
39
47
50
50
48
40
48
43
44
45
45
39
56
45
48
50
49
47
35
39
42
41
40
42
46
37
39
36
46
40
45
43
35
42
43
46
45
30
37
45
47
36
40
39
46
30

50
40
30
20
10
0
Barbus sp

Haplochromis sp

Fish spe

44
33
46
45
42
43
45
41
30
43
35
45
38
50
40
46
35
30
40
38
44
39
40
42
45
40
44
27
46
40
45
40
42
38
40
39
38
40
39
45
40
32
42
38
39
38
37
40

40
32
30
40
40
41
35
40
39
39
34
36
40
39
36
45
42
34
39
38
40
35
35
45
40
40
36
32
35
35
35
35
35
28
29
30
30

Rastineobola
56
53
59
60

to ta l le n g th (m m )

40.29787

80
60
40

to ta l le n g th

60
50
49
50
49
42
43
48
45
52
45
42
40
44
36
39
40
30
35
35
40
40
42
48
43
40
34
35
35
40
42
42
34
35
36
4
34
36
37
35
29
30
36
32
38
45
40
40

80
60
40
20
0

Barbus sp
Hap

30
35
36
34
36
36
34
37
30
27
34
30
32
35
32
35
27
30
36
29
25
30
30
37
32
29
27
38
30
30
29
30
30
29
31
30
27
29
34
28
28
30
25
27
29
29
30
35

25
26
35.96078

Haplochromis sp
Fish species

Nile perch(lates niloticus)

Rastineobola
argentea

min. length
Max length
Average
length(mm)
Column E

Haplochromis sp
fish species

Nile perch(lates niloticus)

Rastineobola
argentea

min. length
Max length
Average
length(mm)

aplochromis sp
Fish species

Nile perch(lates niloticus)

Rastineobola
argentea

min. length
Max length
Average
length(mm)

min. length
Max length
Average
length(mm)

Haplochromis sp
Fish species

Nile perch(lates niloticus)

Rastineobola
argentea

80
60
40

80
60
40
20
0

Barbus sp
Haplochrom
Nile is
perch(lates
sp
Rastineobola
niloticus)
argentea

fish species

min. length
Max length
Average
length(mm)

min. length
Max length
Average
length(mm)

Rastineobola
argentea

obola
a

min. length
Max length
Average
length(mm)
Column E

min. length
Max length
Average
length(mm)

la

min. length
Max length
Average
length(mm)

tea

min. length
Max length
Average
length(mm)

27/10/11

sample
dilution 1:10
ng MC/g fing MC/g fish dry weight
t0
No1 (1:10) 6.944746
6.57932 t0
t0
No2 (1:10) 6.414577 6.000822 t0
t0
No 7 (1:10) 9.513164 9.426504 t0
t1
No3 (1:10) 6.874136 7.253066 t1
t1
No4 (1:10) 5.520393 5.582827 t1
t2
No5 (1:10) 6.041351 5.586894 t2
t2
No6 (1:10) 10.63381 9.669569 t2
t3
No8 (1:10) 5.837646 5.759235 t3
t3
N9 (1:10)
8.032749 7.667403 t3
t4
No10 (1:10 6.280547 6.186188 t4
t4
No11 (1:10 7.208666 7.297717 t4
t4
No12 (1:10 51.86537 44.87146 t4
t0

october
nov
dec
jan
rusinga
baringo

ng MC/ml
64.22934
397.4473
599.3014
230.1754
0
0

stdev
13.88043
188.0854
109.7209
140.763
0
0

ELISA RESULTS OF 16th of November,2011


hours on s Samples
t0
t0
t0
t0
t1
t1
t1
t2

Samples

ng of MC/g
71.38147
552.2265
491.9374
501.3185
705.2848
401.1418
452.9721
851.1652

ng of MC/g fish dry weight


269.1048
490.9653
509.0682
462.3919
499.7312
386.8459
653.8744
857.2384

t2
t2
t3
t3
t3
t4
t4
t4

749.0202
500.2177
391.1833
782.6147
556.7191
917.4372
706.5069
870.4635

754.8651
404.0816
341.6272
731.4873
483.5104
692.3387
575.0903
841.3802

ELISA RESULTS FOR DECEMBER, 13th,2011

Time on Su Samples
t0
t0
t0
t1
t1
t1
t2
t2
t2
t3
t3
t3
t4
t4
t4

Samples

ng of MC/gng of MC/g fish dry weight


603.9444 800.1364
570.2398 471.7953
602.5832 547.1094
443.9399 449.6006
417.0633 471.8655
487.9758 501.0452
302.8312 332.5078
387.5195 385.9095
301.3613 309.4806
312.2099 308.5034
280.1016 301.7834
402.4709 363.7345
347.9814 316.7667
374.225 334.9927
303.5515
260.127

ELISA RESULTS FOR JANUARY, 24TH,2012 : RUSINGA SAMPLES


time on su ng of MC/g ng of MC/g fish dry weight
t0
-36.3986 48.09815
t0
-614.8048 -539.9663
t0
-1036.706 -968.502
t1
18.86955 -23.51857
t1
-182.5215 -275.4141
t1
-246.3624 -258.6582
t2
-342.4717 -299.7419
t2
700.9854 -55.03844
t2
-33.04654 -2.530591
t3
95.75375 78.55248
t3
-217.3546 -338.9516
t3
133.2599 105.8025
t4
-9.895678 -6.737483
t4
-95.34198 -237.6607
t4
-302.4169 -383.4611

ELISA RESULTS FOR JANUARY, 24TH,2012 ,: KISUMU BAY


time on su Samples
t0
t0
t0
t1
t1
t1
t2
t2
t2
t3
t3
t3
t4

ng of MC/g
351.4466
142.8016
174.986
70.30565
103.2405
85.36572
172.3547
214.3763
57.58514
-27.68458
125.3074
241.7587
156.0448

ng of MC/g fish dry weight


458.4994
134.4506
118.868
163.3109
123.5071
115.9375
133.8659
231.6811
158.9674
-54.79835
109.0969
252.8407
118.7193

t4
t4

107.0587 104.8064
141.9146 125.2303

ng MC/g fing MC/g fish dry we dilution 1:10


No1 (1:100 61.64064
52.3389
time on the
No2 (1:100 53.52607 54.90525
t0
No7 (1:100 84.51572 78.44948
t0
No3 (1:100 64.60757 66.84671
t0
No4 (1:100
0 45.21453
t1
No5 (1:100 59.97371 50.15157
t1
No6 (1:100 104.1129 91.25641
t2
No8 (1:100 50.14338 52.69171
t2
No9 (1:100 68.39284 67.66215
t3
No10 (1:10 70.73164 52.61468
t3
No 11 (1:10 64.07208
63.4042
t4
No12 (1:10 5.352182 5.609852
t4
t4

n g o f M C / g fi s h d r y w e i g h t

dil 1:100
t0
t0
t0
t1
t1
t2
t2
t3
t3
t4
t4
t4

Microcystins Concentration in fish (ng


1000
900
800
700
600

ng of M C/

Microcystins Concentration in fish (ng


1000
900
800
700
600
500
400
300
200
100
0

n g o f M C / g fi s h d r y w e i g h t

t0

t0

t0

t0

t1

t1

t1

t2

t2

t2

t3

t3

t3

Sam ple&tim e on sun (hrs)

Microcystins concentration in fish (ng


900
800
700
600
500
400
300
200
100
0

fi s h d ry w e i g h t

t0

t0

t0

t1

t1

t1

t2

t2

t2

t3

t3

drying sam ples on sun (hrs)

t3

n g o f M C /g fi s h d ry w e i g h

Microcystins concentration in
800
600
400
200
0
t0

t0

t0

t1

t1

t1

t2

t2

-200
-400
-600
-800
-1000

n g o f M C / g fi s h d r y w e i g h t

-1200

drying of sam ples

Microcystins concentration in fish (ng MC/g


500
400
300
200

ng of M

Microcystins concentration in fish (ng MC/g


500
400
300
200
100
0
t0
-100

t0

t0

t1

t1

t1

t2

t2

t2

t3

t3

sam ple drying on sun (hrs)

t3

t4

t4

M i c r o c y s t i n s C o n c ( n g M C / g fi s h d r y w e i g h t )

ng MC/g fing MC/g fish dry weight


6.944746
6.57932
6.414577 6.000822
9.513164 9.426504
6.874136 7.253066
5.520393 5.582827
6.041351 5.586894
10.63381 9.669569
5.837646 5.759235
8.032749 7.667403
6.280547 6.186188
7.208666 7.297717
51.86537 44.87146

Microcystins concentration in
60
50
40
30
20
10
0

t0

t0

tration in fish (ng MC/g of fish dry weight)

t0

t1

t1

t2
t2
t3
tim e (hrs)

tration in fish (ng MC/g of fish dry weight)

ng of MC/g fish dry


weight

t2

t2

t3

t3

t3

m e on sun (hrs)

t4

t4

t4

ration in fish (ng MC/g of fish dry weight)

ng of MC /g fish dry
weight

t2

t3

t3

es on sun (hrs)

t3

t4

t4

t4

concentration in fish (ng MC/g of fish dry weight)

t1

t2

t2

t2

t3

t3

t3

drying of sam ples on sun (hrs)

in fish (ng MC/g of fish dry weight)

ng of MC/g fis h dry


weight

t4

t4

t4

Column B
'microcystins
fish'!#REF!

in fish (ng MC/g of fish dry weight)

ng of MC/g fis h dry


weight

t3

t3

un (hrs)

t3

t4

t4

t4

(hrs)
dil 1:10

stins concentration in fish (ng MC/g fish dry weight) with time (hrs)

t0

ng MC /g fish dry
w eight

t1

t1

t2
t2
t3
tim e (hrs)

t3

t4

t4

t4

hours on s samples
samples
t0
1b
1b
t0
2b
2b
t0
3b
3b
t0
4b
4b
t1
5b
5b
t1
6b
6b
t1
7b
7b
t2
8b
8b
t2
9b
9b

ng of MC/g
71.38147
552.2265
491.9374
705.2848
401.1418
452.9721
851.1652
749.0202
500.2177

ng of MC/g fish dry weight


269.1048
490.9653
509.0682
499.7312
386.8459
653.8744
857.2384
754.8651
404.0816

t2
t3
t3
t3
t4
t4
t4

10b
11b
12b
13b
14b
15b
16b

10b
11b
12b
13b
14b
15b
16b

391.1833
782.6147
556.7191
917.4372
706.5069
870.4635
501.3185

time step ng of MC/gng of MC/ghours on s Average


t0
603.9444 800.1364
t0
570.2398 471.7953
0
592.2558
t0
602.5832 547.1094
t1
443.9399 449.6006
2
449.6597
t1
417.0633 471.8655
t1
487.9758 501.0452
4
330.5707
t2
302.8312 332.5078
t2
387.5195 385.9095
6
331.5941
t2
301.3613 309.4806
t3
312.2099 308.5034
8
341.9193
t3
280.1016 301.7834
t3
402.4709 363.7345
t4
347.9814 316.7667
t4
374.225 334.9927
t4
303.5515
260.127

341.6272
731.4873
483.5104
692.3387
575.0903
841.3802
462.3919

Average
606.3471
474.1704
342.6327
324.6738
303.9622

time on s ng of MC/g

eight)

t4

Column B
'microcystins in
fish'!#REF!

t0
t0
t0
t1
t1
t1
t2
t2
t2
t3
t3
t3
t4
t4
t4

time on s ng of MC/gng of MC/g fish dry time on s average


t0
351.4466 458.4994
t0
142.8016 134.4506
t0
223.0781
t0
174.986
118.868
t1
70.30565 163.3109
t1
86.30394
t1
103.2405 123.5071
t1
85.36572 115.9375
t2
148.1054
t2
172.3547 133.8659
t2
214.3763 231.6811
t3
113.1272
t2
57.58514 158.9674

-36.3986
-614.8048
-1036.706
18.86955
-182.5215
-246.3624
-342.4717
700.9854
-33.04654
95.75375
-217.3546
133.2599
-9.895678
-95.34198
-302.4169

average
237.2727
134.2518
174.8381
102.3798

t3
t3
t3
t4
t4
t4

-27.68458
125.3074
241.7587
156.0448
107.0587
141.9146

-54.79835
109.0969
252.8407
118.7193
104.8064
125.2303

t4

135.006

116.252

e (hrs)

sh dry weight
t0

455.2076 442.2174

% relative%
torelative
t0
to Average
t0
%
100
100
100

t1

568.4263 632.6529

124.8719 143.0638 133.9678

t2

546.8071 500.1913

120.1226 113.1098 116.6162

t3

752.257 635.7788

165.2558 143.7707 154.5132

t4

692.7629 626.2875

152.1861 141.6243 146.9052

time on%s relative%


torelative
t0
to t0
t0

100

time on s Average %

100

t0

100

t1

75.92322 78.20116

t1

77.06219

t2

55.81552 56.50768

t2

56.1616

t3

55.98833 53.54586

t3

54.7671

t4

57.7317 50.13006

t4

53.93088

ng of MC/g fish dry time on s average

average

time on s

48.09815
-539.9663
-968.502
-23.51857
-275.4141
-258.6582
-299.7419
-55.03844
-2.530591
78.55248
-338.9516
105.8025
-6.737483
-237.6607
-383.4611

t0

-562.6366 -486.7901

t0

t1

-136.6715 -185.8636

t1

t2

108.489 -119.1036

t2

t3

3.886345 -51.53221

t3

t4

-135.8849 -209.2864

t4

time on%s relative%


torelative
t0
to t0

time on s average %

t0

100

t0

100

t1

38.68778 56.58123

t1

47.6345

t2

66.39173 73.68659

t2

70.03916

t3

50.71193 43.14857

t3

46.93025

100

t4

60.51963

48.9951

t4

54.75737

time on theng MC/g fing MC/g fish dry we


t0
6.944746
6.57932
t0
6.414577 6.000822
t0
9.513164 9.426504
t1
6.874136 7.253066
t1
5.520393 5.582827
t2
6.041351 5.586894
t2
10.63381 9.669569
t3
5.837646 5.759235
t3
8.032749 7.667403
t4
6.280547 6.186188
t4
7.208666 7.297717
t4
51.86537 44.87146

1000

100

verage %
t0

Average %
100

t1

133.9678

t2

116.6162

t3

154.5132

10

1
0

146.9052

verage %

% relative%
torelative
t0
to t0

P r o p o r t io n A c c .t o 0 + 1 s a m p le s ( % )

t4

Char
100
90
80
70
60
50
40
30
20
10
0

t0
t1
t2

t0

time on s Average %

t1

t2
Tim e (hrs

t0

100

24.29125 38.18147

t1

31.23636

-19.28226 24.46714

t2

2.592443

-0.690738 10.58613

t3

4.947694

24.15144 42.99316

t4

33.5723

0 + 1 s a m p le s (% )

100

P ro p o rtio n A c c , to

verage %

100

100
90
80
70
60
50

t0

40

t2

30

t1

20
10
0

t0

t1

t2
Tim e (hrs)

70
60
50

t0

40

t2

30

t1

20
10
0

t0

t1

t2
Tim e (hrs)

g MC/g fish dry we time on su average

October

average

time on %
su relative to t0

0 7.624162 7.335549

t0

100

2 6.197264 6.417946

t1

81.28453

4 8.337579 7.628232

t2

109.3573

6 6.935197 6.713319

t3

90.9634

8 21.78486 19.45179

t4

285.7345

0 455.2076 442.2174
November

2 568.4263 632.6529
4 546.8071 500.1913
6

752.257 635.7788

8 692.7629 626.2875
0 592.2558 606.3471
December

449.6597 474.1704

4 330.5707 342.6327
6 331.5941 324.6738
8 341.9193 303.9622

t2

t1

t2
Tim e (hrs)
t3

P ro p o rto p n A c c , to 0 + 1 s a m p le s (% )

Chart Title

t1
t3
t4

t4

100

P ro p o rto p n A c
t2
t1

t1

t2
Tim e (hrs)

t3

t3

t4

t4

100
90
80
70
60
50
40
30
20
10
0

t0

t1

t0

t1

Tim

t2
t1

t1

t2
Tim e (hrs)

t3

t3

t4

t4

% relative to t0

time on su % average

100

t0

100

87.49102

t1

84.38777

103.9899

t2

106.6736

91.51761

t3

91.24051

300
250
200

265.1715

t4

275.453

150
100

t0

50
0

t0

Average %

Average %

t0

t4

t1
t2
t0

t1

t2
Tim e(hrs)

t3
t3

t4

% average

300

t4

250

200

150

100

t0

t2
t1

t3

50
0

t0

t1

t2

t3

t4

Dilution 1:100

t4

t4

time (hrs)
t0
t0
t0
t1
t1
t2
t2
t3
t3
t4
t4
t4

ilution 1:100
ng MC/g fing MC/g fish dry weight
61.64064
52.3389
53.52607 54.90525
84.51572 78.44948
64.60757 66.84671
0 45.21453
59.97371 50.15157
104.1129 91.25641
50.14338 52.69171
68.39284 67.66215
70.73164 52.61468
64.07208
63.4042
5.352182 5.609852

Concentration of micro

entration of microcystins in fish(ng MC/g fish dry weight)

dry weight)

time (hrs) ng MC/g fing MC/g fish dry weight


t0
61.64064
52.3389
t0
53.52607 54.90525
t0
84.51572 78.44948
t1
64.60757 66.84671
t1
0 45.21453
t2
59.97371 50.15157
t2
104.1129 91.25641
t3
50.14338 52.69171
t3
68.39284 67.66215
t4
70.73164 52.61468
t4
64.07208
63.4042
t4
5.352182 5.609852

time on su average

average

time on %
su relative%
torelative
t0
to t0

t0

66.56081 61.89788

t0

100

100

t1

32.30379 56.03062

t1

48.53274 90.52106

t2

82.04331 70.70399

t2

123.2607 114.2268

t3

59.26811 60.17693

t3

89.04355

t4

46.71864 40.54291

t4

97.2197

70.1894 65.49968

t0

100

t1

69.5269

t2

118.7438

t3

93.13163

t4

67.84454

1 + 0 s a m p le s (% )

time on su % average

P ro p o rtio n A c c .to

lative to t0

120
100
80
60

t0

40
20
0

t0

120

100
80
60

t2
t0

40

t3
t1

t4

20
0

t0

t1

t2

Time (hrs)

t3

t4

Standard Curve of 24/01/12

Std0
Std1
Std2
Std3
Std4
Std5
Control

0
0.15
0.4
1
2
3
0.75

Std0
Std1
Std2
Std3
Std4
Std5
Control

0
0.15
0.4
1
2
3
0.75

Abs 450nmAbs
1.289
1.08
0.784
0.58
0.463
0.371
0.555

450nm
1.239
1.035
0.75
0.592
0.458
0.367
0.52

Abs 450nmAbs 450nm

0.75
0.592
0.458
0.367

Abs 450nmAbs 450nm


Std0
Std1
Std2
Std3
Std4

0
0.15
0.4
1
2

0.71
0.573
0.471

0.686
0.553
0.459

A b so rb 4 5 0 n m

0.784
0.58
0.463
0.371
0.555

f(x) =
R = 0

L
12
10
8
6
4
2
0
0

12
10
8

Std5
Control

3
0.75

0.369

0.378

4
2
0

Abs 450nmAbs 450nm


Std0
Std1
Std2
Std3
Std4
Std5
Control

0
0.15
0.4
1
2
3
0.75

0.71
0.573
0.471
0.369

0.686
0.553
0.459
0.378

A b so rb a t 4 5 0 n m

f(x) =
R = 0

Line

12
10
8
6
4
2
0
0

A b so rb 4 5 0 n m

A b so rb 4 5 0 n m

Linear range for all stds


1.4
1.2
1
0.8
0.6

Abs
450nm

0.4
0.2
0

A b so rb 4 5 0 n m

0.5

1.5

2.5

m icrocystins ng/m l

0.9
0.8
f(x) = - 0.1423979592x + 0.7695867347
R = 0.9525649105

0.7
0.6
0.5

Abs 450nm
Abs 450nm
Linear (Abs
450nm)

0.4
0.3
0.2
0.1
0
0

x) =
=0

0.5

1.5

2.5

Microcystins ng/m l

3.5

Linear range for all stds


12
10
8
Abs 450nm
Abs 450nm
Linear (Abs
450nm)
Linear (Abs
450nm)

6
4
2
0
0

3.5

10

12

A b so rb a t 4 5 0 n m

12
10
8
Abs 450nm
Abs 450nm
Linear (Abs
450nm)
Linear (Abs
450nm)

6
4
2
0
0

x) =
= 0

microcystins ng/m l

10

12

Linear range Stds 2-5

12
10
8

Abs 450nm
Abs 450nm
Linear (Abs
450nm)

6
4
2
0
0

10

m icrocystins ng/m l

12

samples
calc ng/ml
integ
1.582231
net
2.609326
surface
2.124134
patch
2.571519
shore
2.407687
patch diss. 2.067423
surface diss. 2.136736
integ diss.
1.991808
tap water
-1.864524

m ic ro c y s tin s n g /m l

28/10/11
calc ng/ml
1.872086
2.514808
2.004411
2.489603
2.344675
2.124134
2.275362
2.010712
-2.31821

3
2
1
0
-1
-2

15/11/11
samples
patch
surface

ng/ml
ng/ml
159.7658 153.3795
-244.1724 -243.108

M i c ro c y s tin s n g /m l

-3

Microcyst

200
100

0
-100

M i c ro
integ
-211.1762
shore
-230.8675
patch diss -96.75359
surface dis -79.72326
integ diss -104.2044

-300.0532
-286.7483
-93.5604
-153.1666
-152.6344

200
100
0
-100
-200
-300

M ic ro c y s tin s n g /m l

-400

5/12/2011
samples

cal ng/ml cal ng/ml

patch
1.812785
surface
-2.228311
integ
-2.6621
shore
1.461187
diss patch 0.114155
surface dis -0.13242
integ diss
-0.69863

1.721461
-2.47032
-5.127854
1.305936
-0.990868
-0.771689
-1.182648

2
1
0
-1
-2
-3
-4
-5
-6

12/1/2012
site

Sample
ng/ml cal ng/ml cal

Mic

patch

kisumu bay

Rusinga

surface
2.448498
Inte
2.223176
patch
3.746781
shore
3.660944
diss surfac -0.051502
diss inte
0.291845
diss patch 3.263948

2.759657
1.633047
3.832618
3.77897
0.206009
0.248927
3.274678

surface
-5.95279
inte
-6.103004
patch
2.652361
shore
-6.403433
diss surfac -4.311159
diss inte -3.560086
diss patch -1.349785

-5.427039
-5.233906
2.587983
-7.272532
-4.075107
-4.515021
-1.76824

M i c ro c y s tin s n g /m l

m ic ro c y s tin s n g /m l

Microcystins concentration in water (ng/m

2
1
0
-1
-2
-3

Water sam ples

Microcystins Concentration in water (ng/ml)

200
100
0
-100

ng/
ml

M i c ro

200
100
0
-100

ng/
ml

-200
-300
water samples

M ic ro c y s tin s n g /m l

-400

Microcystins concentration in water,(ng/ml)

3
2
1
0
-1

patch

surface

integ

shore

diss
patch

-2
-3
-4
-5
-6

water samples

surface
diss

integ
diss

samples
integ
net
surface
patch
shore
patch diss.
surface diss
integ diss.
tap water

n in water (ng/ml)

calc
ng/ml

Average
INTEG
SURFACE
PATCH
SHORE

ng MC/ml
1.78702
2.130435
2.319471
2.407687

es

samples ng/ml
patch
159.7658
surface
-244.1724
integ
-211.1762
shore
-230.8675
patch diss -96.75359

ng/ml)

ng/
ml

ng/ml
153.3795
-243.108
-300.0532
-286.7483
-93.5604

ng MC/ml
1.941399
2.139887
2.306868
2.344675

surface di -79.72326 -153.1666


integ diss -104.2044 -152.6344
ng/
ml

integr
surface
patch
shore

-157.6903
-161.9478
31.50612
-230.8675

-226.3438
-198.1373
29.90953
-286.7483

samples

patch
1.812785
surface
-2.228311
integ
-2.6621
shore
1.461187
diss patch 0.114155
surface di -0.13242
integ diss -0.69863

ater,(ng/ml)

surface
diss

integ
diss

cal ng/ml cal ng/ml


1.721461
-2.47032
-5.127854
1.305936
-0.990868
-0.771689
-1.182648

cal
ng/ml

integrated -3.894977 -2.949772


surface
-1.180365 -1.621005
patch
0.96347 0.365297
shore
1.461187 1.305936

site
kisumu bay

integrated 1.257511 0.940987


surface
1.198498 1.482833
patch
3.505365 3.553648
shore
3.660944
3.77897

Rusinga

Rusinga
integrated -4.831545 -4.874464
surface
-5.131974 -4.751073
patch
0.651288 0.409871
shore
-6.403433 -7.272532

calc ng/ml
1.582231
2.609326
2.124134
2.571519
2.407687
2.067423
2.136736
1.991808
-1.864524

calc ng/ml
1.872086
2.514808
2.004411
2.489603
2.344675
2.124134
2.275362
2.010712
-2.31821

samples
integrated

1.864209

surface

2.135161 114.5344
2.31317 124.0832

shore

2.376181 127.4632

net

2.562067 137.4345

average of conc ng/ samples

integrated -175.3858

integrated

surface

surface

-180.0426

100

patch

tap water

samples

average n% in relation to int

-2.091367 -112.1852

prop (%)
100
102.6551

patch

30.70782

patch

-17.50872

shore

-258.8079

shore

147.5649

samples

surface
Inte
patch

average of microcyssamples

integrated -2.417808

integrated

surface

surface

-1.400685

prop.(%)
100
57.93201

patch

0.664384

patch

-27.47875

shore

1.383562

shore

-57.2238

ng/ml cal
2.448498
2.223176
3.746781

ng/ml cal
2.759657
1.633047
3.832618

site

samples average micocystins


integrate 1.099249

Kisumu basurface

1.340665

shore
3.660944
3.77897
diss surfac -0.051502 0.206009
diss inte
0.291845 0.248927
diss patch 3.263948 3.274678
surface
-5.95279
inte
-6.103004
patch
2.652361
shore
-6.403433
diss surfac -4.311159
diss inte -3.560086
diss patch -1.349785

-5.427039
-5.233906
2.587983
-7.272532
-4.075107
-4.515021
-1.76824

patch

3.529506

shore

3.719957

Integrate -4.853004
Rusinga

surface

-4.941524

patch

0.530579

shore

-6.837983

100

surface

114.5344

patch

124.0832

shore

127.4632

net

137.4345

tap water

P ro p o rtio n o f m ic ro c y s tin s c o n c . (% )

integrated

% in relation to integrated sample

Proportion of microcystin
200
150
100

-112.1852

50
0
-50
-100
-150

Proportion of microcystins conc. (%)

samples

inte
grat
ed

surf
ace

Proportion of microcystins concentration in samples in relation t


160
140

Proportion of microcysti

p r o p o r t i o n o f m i c r o c y s t i n s c o n c .( % )

Proportion of microcystins concentration in samples in relation t


160
140
120
100
80
60
40
20
0
-20
-40

integ
rated

surfa
patch
ce
water
samples

shore

Proportion of microcystins concentration in samples


120
100
80
60
40
20

-40

integra
ted

-60

water sam ples

-80

verage micocystinssite

samples prop.(%)
integrate
100

Kisumu basurface

surface

121.9619

co n c (% )

-20

o f m ic ro c y s tin

patch

co
338.409

Integrate
Rusinga

surface
patch
shore

100
101.824
-10.93301
140.9021

proportion of m
400
200
0
-200

s u rfa c e

shore

in te g ra te d

321.0835

p ro p o rtio n o f m ic ro c y s tin

patch

roportion of microcystins concentration in samples in relation to integrated sample (%)

inte
grat
ed

surf
ace

patc
h

shor
e

water sam ples

tion in samples in relation to integrated sample (%)

net

tap
wat
er

tion in samples in relation to integrated sample (%)

shore

oncentration in samples in relation to integrated sample.(%)

o f m ic ro c y s tin

co n c (% )

r samples

patch

shore

co

Kisum u bay

site

Rusinga

&

water sam ples

sh o re

p a tch

s u rfa c e

In te g ra te d

sh o re

0
-200

p a tch

200

s u rfa c e

400

in te g ra te d

p ro p o rtio n o f m ic ro c y s tin

proportion of microcystins concentration in samples in relation to integrated sa

o integrated sample (%)

sh o re

elation to integrated sample (%)

31/10/2011
Mondsee Protocol in g/L

Sample name
Shore sample
integr. Sample
integr. Sample
0 meter
integr. Sample
patch sample
patch sample
net sample
Shore sample
0 meter
net sample

chl a ( g/L)
integrated 15.65196 14.34763
surface

62.60784 36.52124

patch

542.6013 495.6454

shore

140.8676

net

29738.72 24782.27

chlorophyll a , 08/11/2011
sample
chl a ug/l
11
integrated
12
integrated sample
13
0 meter sample
14
0 meter sample
15
patch
16
patch
17
shore
18
shore
19
net
20
net

114.781

integrated
surface
patch
shore
net

Chlorophyll a, 14/12/11

11
12
13
14
15
16
17
18
19
20

Chl a ug/l Vol filtered


300
300
300
300
100
100
50
50
5
5

(ml)
inte
inte
0m
0m
shore
shore
patch
patch
net
net

14/1/12 RUSINGA SAMPLES


surface

chl a ug/l
15.65196 10.43464

integ

18.26062 10.43464

shore

13.0433 16.95629

patch

866.0751 881.7271

net

939.1176 1043.464

chl a ug/l
integrated
surface

12.17375
26.0866

patch

1627.804

shore

125.2157

net

5112.974

chl a (g/l)

Samples

10000

1000
100
10
1

su

100
10
1

14/1/12 KISUMU BAY SAMPLES


Samples
0m
11
12
inte

13
14

shore 15
16
patch 17
18
net

19
20

chl a ug/l
45.21677
43.47767
33.04303
26.0866
1763.454
1711.281
4904.281
3860.817
1721.716
1721.716

inte

chl a ug/l
33.04303

26.0866

surface

45.21677 43.47767

shore

1763.454 1711.281

patch

4904.281 3860.817

net

1721.716 1721.716

su
a

chl a (g/l)

13.0433

chlorophyll a concentration (g/l)

100000
10000
1000
100
10
1

integ
rated

surfa
ce

patc
shor
h
e
water samples

14.6085 18.78235
16.69542 22.95621
286.9526

260.866

33.91258

2.60866

799.9891 208.6928

chl a (g/l)

chl a ug/l

1000

chlorophyll a concentrat

100
10
1

inte
grat
ed

surf
ace

patc
h

water samples

chl a (g/l)

chl a ug/l

6.956427

inte
grat
ed

surf
ace

patc
h

water samples

chlorophyll a concentra
10000
1000
100

12.17375

10

62.60784

20.86928
2086.928

water sample

10000

Chlorophyll a concentration (g/l)

1000
100
chl a
ug/l

10
1

surf

inte

shor

patc

net

100
chl a
ug/l

10

surf
ace

inte
shor
patc
g
e
h
water samples

chl a (g/l)

10000

net

Chlorophyll a concentration (g

1000
100
10
1

inte

surf
ace

shor
patc
e
h
water samples

sample
concentration ( g/L)
integrated
14.34763

ion (g/l)

surface

49.56454

patch

519.1233

scum

127.8243

net

27260.5

chl a (
g/L)

shor
e

net

a concentration (g/l)

urf
ce

samples

chl a
ug/l

patc
h

water samples

shor
e

net

chl a ug/l

Integrated

16.69542

surface

19.82582

patch

273.9093

scum

18.26062

net

504.3409

urf
ce

patc
h

shor
e

net

water samples

a concentration (g/l)
Chl a ug/l

chl a
ug/l

water samples

Sample
integ
surface

chl a ug/l
14.34763
13.0433

Sample
integ

integrated

9.565087

surface

19.13017

patch

845.2058

scum

73.04248

net

3599.951

% relative to integrated sample


100

surface

90.90909

patch

873.9011

patch

6090.909

scum

14.9998

scum

104.5455

net

6909.091

net

991.2908

ntration (g/l)

mples

patc
h

net

Samples chl a ug/l


0m
11 45.21677
12 43.47767
inte
13 33.04303
14
26.0866
shore 15 1763.454
16 1711.281
patch 17 4904.281
18 3860.817
net
19 1721.716
20 1721.716

proportion (%)

% in relation to integrated sample


integrated
100
surface

345.4545

patch

3618.182

shore

890.9091

200000
150000

190000

100000
50000
0

samples
Integrated
surface

% relative to integ
100
118.75

patch

1640.625

shore

109.375

net

3020.833

proportion (%)

net

Propo

in
at

Proportion of
3500
3000
2500
2000
1500
1000
500
0

Integ
rated

500

samples

Chl a ug/l

samples

% relative to integ

9.565087

integrated

100

surface

19.13017

surface

200

patch

845.2058

patch

8836.364

shore

73.04248

shore

763.6364

net

3599.951

net

37636.36

proportion (%)

integrated

Proportion (%)

Integ
rated

Propor
40000
35000
30000
25000
20000
15000
10000
5000
0

proportion of chlorophyll a in samples in relation to


8000
7000
6000
5000
4000
3000
2000
1000

7000
6000
5000
4000
3000
2000
1000
0

samples

integ

Average chl%a rel


( to integ.

surfac
e

samples

patch
water samples

% rel to integ.

integrated

29.56481

100

integrated

100

surface

44.34722

150

surface

150

patch

4382.549 14823.53

patch

14823.53

shore

1737.368 5876.471

shore

5876.471

net

1721.716 5823.529

net

5823.529

scum

proportion (%)

Proportion of chlorophyll a in samples in relation to integrated samp


200000
150000
100000
50000
0

integr
ated

surfac
e

patch

shore

net

water samples

Proportion of chlorophyll a in samples in relation to integrated sample (%)

Integ
rated

surfa
ce

patch
water samples

shore

net

Proportion (%)

Integ
rated

surfa
ce

patch

shore

net

water samples

Proportion of chlorophyll a in samples in relation to integrated samp


40000
35000
30000
25000
20000
15000
10000
5000
0

integ
rated

surfa
ce

patch
Water samples

s in relation to integrated sample(%)

shore

net

scum

net

Proportion (%)

Proportion of chlorophyll a in samples in relation


16000
14000
12000
10000
8000
6000
4000
2000
0

integ
rate
d

surfa
ce

patc
h

water samples

shor
e

ne

tegrated sample (%)

net

d sample (%)

tegrated sample(%)

net

mples in relation to integrated sample (%)

shor
e

net

ELISA

27/10/11 (dil: 1:100)


time (hrs) ng MC/g fish dry weight
0
0 45.21453
0 5.352182 5.609852 5.481017
0 50.14338 52.69171 51.41754
2 53.52607 54.90525 54.21566
2 59.97371 50.15157 55.06264
2 61.64064
52.3389 56.98977
4 64.07208
63.4042 63.73814
4 64.60757 66.84671 65.72714
4 68.39284 67.66215
68.0275
6 70.73164 52.61468 61.67316
6 84.51572 78.44948
81.4826
6 104.1129 91.25641 97.68466
71.38147 269.1048 170.2431
391.1833 341.6272 366.4053
401.1418 386.8459 393.9939
452.9721 653.8744 553.4233
491.9374 509.0682 500.5028
500.2177 404.0816 452.1497
501.3185 462.3919 481.8552
552.2265 490.9653 521.5959
556.7191 483.5104 520.1147
705.2848 499.7312
602.508
706.5069 575.0903 640.7986
749.0202 754.8651 751.9426
782.6147 731.4873
757.051
851.1652 857.2384 854.2018
870.4635 841.3802 855.9218
917.4372 692.3387 804.8879
280.1016 301.7834 290.9425
301.3613 309.4806
305.421
302.8312 332.5078 317.6695
303.5515
260.127 281.8393
312.2099 308.5034 310.3567
347.9814 316.7667 332.3741
374.225 334.9927 354.6089
387.5195 385.9095 386.7145
402.4709 363.7345 383.1027
417.0633 471.8655 444.4644
443.9399 449.6006 446.7702
487.9758 501.0452 494.5105
570.2398 471.7953 521.0176
602.5832 547.1094 574.8463
603.9444 800.1364 702.0404
57.58514 158.9674 108.2763
70.30565 163.3109 116.8083

ng MC/g fing MC/ml


0.738861 0.919742
1.711111 0.940242
1.734125 0.225568
1.740857 0.334132
1.755797 2.867539
1.804399 2.574004
1.817745 2.445142
1.832684 2.395504
1.790096 3.205731
1.911065
3.35466
1.989826 0.261025
2.23107 0.261025
2.563962 0.463893
2.595489 0.471614
2.743057 1.623649
2.699407 2.301324
2.655282 1.335799
2.682917 1.314221
2.717334 3.400599
2.716099 2.978368
2.779963 0.766086
2.806722 0.269536
2.876185 0.203115
2.879125 0.262688
2.93156 0.183953
2.932434 0.796789
2.905735 3.562947
2.463807 3.149083
2.484899 2.414868
2.501976 2.507158
2.450002 0.296577
2.491861 0.236134
2.521627 0.220814
2.54975 0.648818
2.58739 0.876021
2.583315 3.448011
2.647837 3.449742
2.650084 1.654671
2.694176 1.669713
2.716852 1.150348
2.759552 1.013136
2.846362
2.034533
2.067474

85.36572
103.2405
107.0587
125.3074
141.9146
142.8016
156.0448
172.3547
174.986
214.3763
241.7587
351.4466

115.9375
123.5071
104.8064
109.0969
125.2303
134.4506
118.7193
133.8659
118.868
231.6811
252.8407
458.4994

100.6516
113.3738
105.9325
117.2022
133.5724
138.6261
137.3821
153.1103
146.927
223.0287
247.2997
404.973

2.002821
2.054513
2.025029
2.068936
2.125717
2.141845
2.13793
2.185004
2.167102
2.348361
2.393224
2.607426

16th/11/1 (dil, 1:1000)


time (hrs) ng of MC/gng of MC/g fish dry weight
0 71.38147 269.1048
0 391.1833 341.6272
0 401.1418 386.8459
0 452.9721 653.8744
2 491.9374 509.0682
2 500.2177 404.0816
2 501.3185 462.3919
4 552.2265 490.9653
4 556.7191 483.5104
4 705.2848 499.7312
6 706.5069 575.0903
6 749.0202 754.8651
6 782.6147 731.4873
8 851.1652 857.2384
8 870.4635 841.3802
8 917.4372 692.3387

13/12/11 (dil, 1:1000)


time step
0
0
0
2
2
2
4
4
4
6
6
6
8
8
8

ng of MC/gng of MC/g fish dry weight


280.1016 301.7834
301.3613 309.4806
302.8312 332.5078
303.5515
260.127
312.2099 308.5034
347.9814 316.7667
374.225 334.9927
387.5195 385.9095
402.4709 363.7345
417.0633 471.8655
443.9399 449.6006
487.9758 501.0452
570.2398 471.7953
602.5832 547.1094
603.9444 800.1364

24/01/12 (Rusinga) (dil, 1:1000)

24/01/12

time on s ng of MC/gng of MC/g fish dry weight


t0
-36.3986 48.09815
t0
-614.8048 -539.9663
t0
-1036.706 -968.502
t1
18.86955 -23.51857
t1
-182.5215 -275.4141
t1
-246.3624 -258.6582
t2
-342.4717 -299.7419
t2
700.9854 -55.03844
t2
-33.04654 -2.530591
t3
95.75375 78.55248
t3
-217.3546 -338.9516
t3
133.2599 105.8025
t4
-9.895678 -6.737483
t4
-95.34198 -237.6607
t4
-302.4169 -383.4611

time on s
0
0
0
2
2
2
4
4
4
6
6
8
8
8

(Kisumu bay)

(dil, 1:1000)

ng of MC/gng of MC/g fish dry time (hrs)


57.58514 158.9674
70.30565 163.3109
85.36572 115.9375
103.2405 123.5071
107.0587 104.8064
125.3074 109.0969
141.9146 125.2303
142.8016 134.4506
156.0448 118.7193
172.3547 133.8659
174.986
118.868
214.3763 231.6811
241.7587 252.8407
351.4466 458.4994

RESULTS

Physco-chemical data
fish composition (total length, fresh weight & species composition)
chlorophyll a concentration (g/l)
ELISA results of water samples for all the sampling dates.
ELISA results for fish samples
( graphs of ng MC/g fish dry weight), prop (%) in relation
HPLC water sample results ( conc, ng/ml), relation with microcystis cell density, ng MC /ml & dry w
Calibartion curve
phytoplankton results
conductivity
sampling site date
S/cm
STD
27.10.11
152.9 0.387298
Kisumu bay

Rusinga

8.11.11

158.8714

0.11127

5.12.11

130 0.305505

10.1.12

134.2 0.305505

9.1.12

106.1143 0.106904

CHLOROPHYLL a
Integrated sample
sampling site Date
Kisumu bay

Rusinga

27.10.11

chl a ( g/l)chl a ( g/L chl a ( g/L)


15.65196 14.34763
13.0433

8.11.11

14.6085 18.78235

5.12.11

12.17375 6.956427

10.1.12

33.04303

9.1.12

18.26062 10.43464

26.0866

chl a (g/l)

100

10

1
27.10.11

8.11.11

5.12.11
Kisumu bay
Sampling site & date

Microcystin Concentrationwith ELISA


Integrated sample
sampling site Date
Kisumu bay

Rusinga

27.10.11

ng MC/ml ng MC/ml
1.78702 1.941399

8.11.11

-157.6903 -226.3438

5.12.11

-3.894977 -2.949772

10.1.12

1.257511 0.940987

9.1.12

-4.831545 -4.874464

-39.4657902

ng MC/ml

Microcystin Concentration (ng MC/ml)


10

10.1.12

10

Kisumu bay

9.1.12

10.1.12

5.12.11

8.11.11

27.10.11

1
ng
MC/ml

Rusinga

Sampling site & date

ELISA
average MCs cstd dev
x fold integrated sample
1.481729
0.46446
1

integrated
surface

1.73791296 0.473177 1.172895

patch

7.549

12.268 5.094724

shore

2.493

1.05 1.682494

samples
integrated

x fold integrated sample


1

surface

1.172895287

patch

5.094723799

shore

1.682493897

Chart Title
ld intergrated sample

ng MC/ml

Microcystin Concentration (ng MC/ml)

10

x fold intergrate

Samples

FISH SAMPLES
Kisumu bay

fish composition
Barbus sp
Haplochromis sp
Nile perch
Rastineobola
fish species
Barbus sp
Haplochromis s
Nile perch
Rastineobola a

Average
16
2
12
78.25
108.25

14.7806004619
1.8475750577
11.0854503464
72.2863741339

%
14.9
1.8
11.1
72.3

Barbus sp
Haplochromis sp
Nile perch
11%
Rastineobola argentea
72%

FRESH WEIGHT(g)
Kisumu bay
fish specie average FW
Barbus sp

1.089825

SD
0.23455774

Haplochromi

0.6 0.353553391

Nile perch(la

0.639075 0.145911466

Rastineobol

0.655 0.164909066
Rastineobola Rastineobo Nile perch Nile perch
average
SD
avergarge SD
Kisumu bay
0.655
0.164
0.639
Rusinga

Kisumu bay

0.469
0.213
1.833
0.463
Average
Rastineobola Nile perch (Barbus spp Haplochromis spp.
0.655
0.639
1.09
0.6

Rusinga

0.469

Kisumu bay

0.213

total length
kisumu bay
fish species average length stdev
47.800657895

Haplochromi

1.833

1.2

STDEV
Rastineobola Nile perch Barbus
Haplochromis
0.164
0.146
0.235
0.354

Rusinga

Barbus sp

0.146

6.54253304

31.75 5.303300859

Nile perch(la34.078333333 2.803147041


Rastineobol 40.025196078 4.585001781

0.463

microcystins in fish
sa

Rusinga
Baringo

ng MC/g fis stdev


64.22934 13.88043
397.4473 188.0854
599.3014 109.7209
230.1754
140.763
0
0
0
0

4.9745709628
30.7823433807
46.4159728098
17.8271128467

1291.153434 Sampling sitSampling date

Kisumu bay27.10.11
8.11.11
5.12.11
10.01.12
Rusinga

9.01.12

Baringo

20.11.11

Microcy
1000
ng MC/g fish dry weight

Kisumu bay

27.10.11
8.11.11
5.12.11
10.01.12
9.01.12
20.11.11

100

10

1
0

08.11.12

Probe/sample

5A
6A
6
8
11
14

5.12.11

10.1.12

ght), prop (%) in relation to t0.


nsity, ng MC /ml & dry weight of the sample

9.1.12
Rusinga

Surface
sampling site
Kisumu bay

Rusinga

10
g MC/ml

ate

10.1.12

ng MC/ml
10

Barbus sp
Haplochromis sp
Nile perch
11%
argentea

Barbus
SD

Haplochromis
average

1.09

0.235

1.2

Average fresh weight of fish specimen (g)

Barbus
average

3.5

Haplochromis
SD
Average fresh weigh
0.6
0.354
0

3
2.5
2
1.5
1
0.5
0
Kisumu bay

Sampl

Rusinga
Fish species
Barbus sp
Haplochromis sp
Nile perch(Lates niloticus)
Rastineobola argentea

ng MCYST/g fish dry weight


Mean SD
64.23 13
397.45 188
599.30110
230140
-

Microcystin concentration in fish samples with time of exposure on s

ng MC/g fish dry weight

1000

100

10

1
0

Time (hrs)

Microcystin concentration in fish samples with tim

MCYST - desm LR
(ng/ml extract)

MCYST - YR
(ng/ml extract)

6021
15900

100

119
833

10

5178
24236
107
89
49
44

ng of
weig

1
0

Time (hrs)

Microcystin concentration in fish samples with time o


1000

ng MC/g fish dry weight

26
48

ng MC/g fish dry weight

MCYST
- LR
1000
(ng/ml extract)

100
ng of MC/g
weight

10

1
0

Time (hrs)

Microcystin concentration in fish samples with tim


1000
ight

MCYST - RR
(ng/ml extract)

Microcystin concentration in fish samples with tim

ng MC/g fish dry weight

1000

100

ng
we

10

1
0

Time (hrs)

C o n d u c tiv ity ( S /c m )

Conductivity (S/cm)

1000
100

Kisum u bay

Sam pling site & Date

surface
sampling site

Date

Kisumu bay 27.10.11

Rusinga

chl a ( g/L chl a ( g/L)


62.60784 36.52124

8.11.11

16.69542 22.95621

5.12.11

26.0866 12.17375

10.1.12

45.21677 43.47767

9.1.12

15.65196 10.43464

9 .1 .1 2

1 0 .1 .1 2

5 .1 2 .1 1

8 .1 1 .1 1

2 7 .1 0 .1 1

10

Rusinga

Kisumu bay

9.1.12

10.1.12

5.12.11

8.11.11

10

27.10.11

Chl a (g/l)

100

Rusinga

Sampling site & Date

Date
27.10.11

ng MC/ml
ng MC/ml
2.1304348 2.139887

8.11.11

-161.94784 -198.1373

5.12.11

-1.1803653 -1.621005

10.1.12

1.1984979 1.482833

9.1.12

-5.1319742 -4.751073

Microcystin Concentration (ng MC/ml)

.12

.12

.11

.11

.11

g MC/ml

10

ng

Microcystin Concentration (ng MC/ml)

9.1.12

10.1.12

5.12.11

8.11.11

Kisumu bay

ng
MC/ml

Rusinga

Sampling site & date

HPLC
Average MCstd deviati x fold integrated sample
integrated

1.684094

Err:504

surface

3.794687

Err:504 2.253251

patch

651.1227

Err:504 386.6308

shore

60.17281

Err:504 35.73007

samples

x fold integrated sample

integrated

surface

2.253251

patch

386.6308

shore

35.73007

Chart Title
integrated sample

27.10.11

ng MC/ml

10

1000
100
10
1

x fold integrated sam

100
10
1

Samples
Rusinga fish samples
41153
fish specie
Barbus sp
Nile perch(la
Rastineobol

%
0.8
4.5
96.2

Barbus sp Nile perch(lates niloticus)

Rastineobola argentea

total

41153

Rusinga Fish Samples

Fish species
min. wet w Max wet weAverage we
1.2
1.2
1.2

Barbus sp
Haplochromis sp

Nile perch(lates nilotic

1.3

2.3

1.8333

Rastineobola argentea

0.1

1.5

0.469

rage fresh weight of fish specimen (g)

bay

Column G
Haplochromis spp.
Barbus spp.
Nile perch (Lates
niloticus)
Rastineobola
argentea

Sampling sites

Rusinga

min. length Max length Average le stdev


56
56
56

ch(Lates niloticus)

50

61

57.67 4.320494

obola argentea

27

56

40.3 5.323458

ng MC/g fish dry weight

1000

100

10

10.01.12

5.12.11

8.11.11

27.10.11

Kisumu bay

Sampling site & Date

exposure on sun/open air.

Ru

amples with time of exposure to sun/open air

ng of MC/g fish dry


weight

ples with time of exposure to the sun/open air

ng of MC/g fish dry


weight

amples with time of exposure on the sun/open air

amples with time of exposure on the sun/open air

ng of MC/g fish dry


weight

ay

9 .1 .1 2

1 0 .1 .1 2

S/cm)

Rusinga

Date

patch
sampling site

Date

Kisumu bay27.10.11

Rusinga

chl a ( g/
542.6013

8.11.11

286.9526

5.12.11

1627.804

10.1.12

4904.281

9.1.12

866.0751

5.12.11

8.11.11

27.10.11

Chl a (g/l)

10000
1000
100
10
1

Kisumu bay

Sampling site &

patch
sampling siDate
ng MC/ml ng MC/ml
2.319471 2.306868

Kisumu bay27.10.11

Rusinga

8.11.11

31.50612 29.90953

5.12.11

0.96347 0.365297

10.1.12

3.505365 3.553648

9.1.12

Microcystin Concentration (ng MC/ml)

10
1.12

1.12

2.11

0.1

1.11

1
0.11

MC/ml

100

Microcystin Concentration (ng MC/ml)

10

Kisumu bay

9.1.12

10.1.12

5.12.11

0.1

8.11.11

1
27.10.11

ng MC/ml

100

Rusinga

Sampling site & date

ng
MC/ml

Rusinga

kisumu bay

fish specie
Barbus sp
STD
0

Haplochrom
Nile perch(l

0.463321

Rastineobo

0.213018
Average
Rastineobo
Kisumu bay 40.0252
Rusinga

40.3

20.11.11

Rusinga

Baringo

Sampling site & Date

27.10.11

8.11.11

p ro p , o f M C Y S T C o n c , in re la tio n to t0 (% )

9.01.12

10.01.12

5.12.11

sumu bay

10

1
1.75

1.8

1.85

1.9

1.95

tim e (hrs)

2.05

10.1.12

C Y S T C o n c , i n r e l a t i o n t o Pt 0r o( p% , ) o f M C Y S T C o n c , i n r e l a t i o n t o

5.12.11

t0 (% )
p ro p , o f M C Y S T C o n c , in re la tio n to t0 (%
10

1
0
2
4

10

0
2
4

tim e (hrs)

time (hrs)

tim e (hrs)

10

p ro p , o f M C Y S T C o n c , in
1
0
2
4

tim e (hrs)

6
8

shore
sampling site
chl a ( g/L)
495.6454

Kisumu bay

260.866
262.6078
3860.817
881.7271

Rusinga
chl a (g/l)

Kisumu bay

Sampling site & Date

1.12

ntration (ng MC/ml)

1.12

9.1.12

10.1.12

5.12.11

8.11.11

27.10.11

00
00
00
10
1

Rusinga

10.1.12

9.1.12

ntration (ng MC/ml)

Rusinga

& date

ng
MC/ml

isumu bay

Kisumu bay
Fish species
Barbus sp
Haplochromis sp
Nile perch
Rastineobola argentea

Rusinga

%wt
21
1.3
22
56
100.3

Average to

average le stdev

Fish species

47.80066 6.542533

Barbus sp

31.75 5.303301

56

Haplochromis sp

Nile perch(lates nilotic

57.67

40.0252 4.585002

Rastineobola argentea

40.3

A v e r a g e t o t a l l e n g t h o f fi s h s p e c i m e n i n s a m p l e s ( m m )

34.07833 2.803147

Nile perch (Barbus spp Haplochromis spp.


34.07833 47.80066
31.75
57.67

56

Average total length of fish specimen in fish samples (mm)


180
160
140
120
100
80
60
40
20
0

Haplochromis spp.
Barbus spp.
Nile perch (Lates
niloticus)
Rastineobola
argentea

Kisumu
bay

Rusinga

Sam pling sites

2.445
1416
435

1.9

1.95

m e (hrs)

2.05

tim e (hrs)

time (hrs)

tim e (hrs)

10

10

10

12

12

12

tim e (hrs)

10

12

ampling site
Date

hl a (g/l)

27.10.11

chl a ( g/L chl a ( g/L chl a ( g/L)


140.8676
114.781

8.11.11

33.91258 12.60866

5.12.11

125.2157 50.86928

10.1.12

1763.454 1711.281

9.1.12

13.0433 16.95629

10000
100

10.1.12

8.11.11

5.12.11

10
27.10.11

Chl a (g/l)

1000

Kisumu bay

Sampling site & Dat

shore
sampling siDate
Kisumu bay27.10.11

Rusinga

ng MC/ml ng MC/ml
2.407687 2.344675

8.11.11

-230.8675 -286.7483

5.12.11

1.461187 1.305936

10.1.12

3.660944

9.1.12

3.77897

-6.403433 -7.272532

Microcystin Concentration (ng MC/ml)

9.1.12

0.1.12

.12.11

.11.11

.10.11

ng MC/ml

10

ng
MC/ml

integrated surface

27.10.11

27.10.11
8.11.11
5.12.11
10.1.12
Rusinga

Kisumu bay

27.10.11
8.11.11
5.12.11
10.1.12
Rusinga

100

27.10.11
8.11.11
5.12.11
10.1.12
Rusinga

9.1.12

10.1.12

5.12.11

8.11.11

27.10.11

ng MCYST/ml

ng MC/ml

Microcystin Concentration (ng MC/ml)

10

ng
MC/ml

Rusinga

Sampling site & date

Microcystin Concentration

10

patch

sampling dates & wa

Rusinga
Fish species
Barbus sp
Haplochromis sp

Nile perch(lates nilotic

1.3

Rastineobola argentea

0.1

STDEV

min. wet w
1.2

0
0
4.3
5.3

STDEV
Rastineobo Nile perch (Barbus spp Haplochomis spp.
Kisumu bay 4.585002 2.803147 6.542533 5.303301
Rusinga

n fish samples (mm)

p.

5.3

4.3

surface

9.1.12
Rusinga

5.12.11

8.11.11

Sampling site & Date

10000
1000
100
10
1
27.10.11

chl, a (g/l)

10.1.12

5.12.11

8.11.11

Kisumu bay

Inte

27.10.11
8.11.11
integrated 5.12.11
10.1.12
Rusinga

surface

/ml)
patch

ng
MC/ml

27.10.11
8.11.11
5.12.11
10.1.12
Rusinga
27.10.11
8.11.11
5.12.11

/ml)

10.1.12
Rusinga
ng
MC/ml

shore

surface

patch

27.10.11
8.11.11
5.12.11
10.1.12
Rusinga

27.10.11
8.11.11
5.12.11
10.1.12
Rusinga

8.11.11
5.12.11
10.1.12
Rusinga

tin Concentration in dissolved form

shore

ampling dates & water samples (duplicates)

27.10.11
8.11.11
5.12.11
10.1.12
Rusinga

Max wet weAverage weSTD


1.2
1.2

%wt
1.2 1.673874

2.3

1.8333 0.463321

10.8 15.06486

1.5

0.469 0.213018

59.69 83.26126
71.69

chl a ( g/L chl a ( g/L


27.10.11

15.65196 14.34763

Integrated 8.11.11

14.6085 18.78235

5.12.11

12.17375 6.956427

10.1.12

33.04303

9.1.12

18.26062 10.43464

27.10.11

62.60784 36.52124

26.0866

26.0866 12.17375

10.1.12

45.21677 43.47767

9.1.12

15.65196 10.43464

27.10.11

542.6013 495.6454

8.11.11

286.9526

260.866

1627.804
Chlorophyll5.12.11
a (g/l)

262.6078

Integrated

8.11.11

surface

140.8676

27.10.11

27.10.11

9.1.12

866.0751 881.7271

10.1.12

9.1.12

5.12.11

4904.281 3860.817

8.11.11

10.1.12

27.10.11

shore

10.1.12

5.12.11

8.11.11

9.1.12

10.1.12

5.12.11

8.11.11

27.10.11

10000
1000
100
10
1

27.10.11

patch

114.781

33.91258 12.60866

patch

5.12.11

125.2157 50.86928

10.1.12

1763.454 1711.281

Sampling dates & water samples (duplicates)


9.1.12

Dissolved form(ELISA)
ng MC/ml ng MC/ml
1.78702 1.941399
0
0
0
0
1.257511 0.940987
0
0
2.130435 2.139887
0
0
0
0
1.198498 1.482833
0
0
2.319471 2.306868
31.50612 29.90953
0.96347 0.365297

13.0433 16.95629

10.1.12

5.12.11

5.12.11

16.69542 22.95621

8.11.11

8.11.11

9.1.12

surface

shore

3.505365 3.553648
0
0
2.407687 2.344675
0
0
1.461187 1.305936
3.660944
3.77897
0
0

chl a ( g/L)
27.10.11

62.60784 36.52124

8.11.11

16.69542 22.95621

5.12.11

26.0866 12.17375

10.1.12

45.21677 43.47767

9.1.12

15.65196 10.43464

13.0433

atch

(duplicates)
shore
9.1.12

10.1.12

5.12.11

8.11.11

27.10.11

9.1.12

Integrated
surface

9.1.12

10.1.12

5.12.11

8.11.11

27.10.11

9.1.12

10.1.12

5.12.11

8.11.11

27.10.11

9.1.12

10.1.12

5.12.11

8.11.11

27.10.11

10000

1000

100

10

patch

face
patch
shore
9.1.12

10.1.12

5.12.11

8.11.11

27.10.11

9.1.12

10.1.12

5.12.11

8.11.11

27.10.11

9.1.12

chl a (
g/L)

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