Professional Documents
Culture Documents
two phases, one stationary (stationary phase), the other (the mobile phase) passing in a definite
direction. The prime is the mobile phase, giving up the column. The island is the solvent that
bearing the analyzes.
There are twelve types are:
(1) Column Chromatography
(2) Paper Chromatography
(3) Thin Layer Chromatography
(4) Gas Chromatography
(5) High Performance Liquid Chromatography
(6) Fast Protein Liquid Chromatography
(7) Supercritical Fluid Chromatography
(8) Affinity Chromatography
(9) Reversed Phase Chromatography
(10) Two Dimensional Chromatography
(11) Pyrolysis Gas Chromatography and
(12) Counter Current Chromatography.
chromatography (GC)
is
the
General
type
of chromatography used
in analytical
chemistry for separating and analyzing elements that can be vaporized barring decomposition.
Typical uses of GC comprise testing the purity of a particular substance, or separating the
different elements of a mixture (the relative amounts of such components can also be
determined). In some condition, GC may help in identifying a compound. In preliminary
chromatography, GC can be used to prepare pure compounds from a mixture.
In this gas chromatography, the mobile phase (or "moving phase") is a carrier gas, usually
an inert gas such as helium or an unreactivegas such as nitrogen. Helium remains the most
usually used carrier gas in about 90% of instruments although hydrogen is introduce for