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Reproduction in Organisms
Asexual Reproduction
The period through which a certain organism lives is known as its life
span.
Reproduction is the process by which every organism ensures its
continuity.
It is the process through which organisms produce young ones, which
in turn mature to give rise to their young ones.
Reproduction can be:
o Asexual Only one individual is involved
o Sexual Two individuals (male and female) are involved
Asexual Reproduction
In this type, a single parent can produce offspring.
The produced offspring are clones of each other (i.e., identical to
each other and to the parent).
It is commonly seen in unicellular organisms belonging to protista and
monera.
Here, the cell division itself is the mode of reproduction.
Means of Asexual Reproduction
Binary Fission In this process, the cell divides into halves, and
each half develops into an adult (example: Amoeba, Paramecium).
Budding In this process, the cell divides unequally to form buds,
which remain attached to the parent initially, and then detach and
develop into a mature cell (example: yeast).
Formation of specialized structures
o Conidia (Example: Penicillium)
o Gemmules (Example: Sponges)
o Buds (Example: Hydra)
o Zoospores Microscopic, motile spores (Example: Algae)
Gametogenesis
Process of formation of gametes (male and female)
Gametes are haploid
In some organisms (like algae), they are almost similar (homo or
isogametes), and cannot be categorised as male and female
gametes.
In others, the two gametes are morphologically and physiologically
different (heterogametes), and are of two typesantherozoid or
sperm (male gamete) and egg or ovum (female gamete).
In some organisms both the sexes are present in the same individual
(monoecious or homothallic), and in others, they are present in two
individuals (dioecious or heterothallic).
In a unisexual flower, the male flower is called staminate and the
female flower is called pistillate.
Gamete formation takes place by cell division.
In haploid parents, it is by mitosis; in diploid parents, it is by meiosis,
with specialised cells called meiocytes undergoing meiosis.
.Gamete Transfer
For their fusion to take place, the gametes need to be transferred.
In most organisms, the male gametes are motile, while the female
gametes are non-motile, and the male gametes need a medium for
their movement. A large number of male gametes do not make it to
the female gamete, and hence, several thousands of male gametes
are produced to overcome this loss.
In angiosperms, the pollen grain carries the male gamete and the
ovule carries the female gamete.
Pollen grains are produced in the anther and need to be transferred
to the stigma for fertilisation to occur. This is easy in monoecious
plants as both the anther and the stigma are present close by; in
dioecious plants, it takes place by pollination.
Sexual Reproduction: Fertilisation Events
Fertilisation is the most important event in sexual reproduction.
This process is also called syngamy and leads to the formation of
the zygote.
Androecium
The androecium consists of whorls of stamen.
The stamen consists of the filament (long and slender stalk) and
anther (bilobed structure).
Filament is attached to the thalamus or to the petal.
Anther:
Structure of microsporangium
o The microsporangium is surrounded by four wall layers
(epidermis, endothecium, middle layers, and tapetum).
o The outer three layers are protective and help in dehiscence of
anther to release the pollen grains. The tapetum provides
nourishment to the developing pollen grains.
o In the young anther, the sporogenous tissue forms the centre of
each microsporangium.
Microsporogenesis
It is the process of formation of microspore from PMC (Pollen Mother
Cells).
As development occurs in the anther, the sporogenous tissue
undergoes meiosis to form microspore tetrad.
Each cell of sporogenous tissue has capacity to give rise to a tetrad.
Hence, each cell is a potential pollen or PMC.
As the anther matures, the microspores get detached from each other
and develop into pollen grains.
Pollen grains
Represent the male gamete and are spherical, having a two-layered
wall:
o Exine (outer) Hard layer made of sporopollenin, which is
extremely resistant and can withstand high temperatures, acidic
and alkaline conditions, and enzymes
o Intine (inner) Thin and continuous layer made up of cellulose
and pectin
Mature pollen grain contains two cells:
o Vegetative cell Large with irregular nucleus, contains food
reserves
o Generative cell Small and floats in the cytoplasm of the
vegetative cell
Megasporangium
Megasporogenesis
The megaspore mother cell (MMC) gets converted into megaspores
by the process of megasporogenesis.
The MMC is large and contains a dense cytoplasm and a prominent
nucleus. It undergoes meiosis to produce four megaspores.
Female Gametophyte
In most flowering plants, only one megaspore is functional while the
other three degenerate.
The single functional megaspore develops into the female
gametophyte. This kind of development is called monosporic
development.
The nucleus of the functional megaspore divides mitotically to form 2
nuclei, which move towards the opposite ends, forming a 2-nucleate
embryo sac. Two more mitotic divisions ensue, leading to the
formation of 4-nucleate and 8-nucleate embryo sacs.
After the 8-nucleate stage, the cell walls are laid down and the typical
female gametophyte (embryo sac) gets organised.
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Six of the 8-nuclei get surrounded by the cell wall and the remaining
two, called polar nuclei, are situated below the egg apparatus in the
large central cell.
Three of the six cells are placed at the micropylar end and constitute
the egg apparatus (2 synergids + 1 egg cell).
The synergids have special thickenings at the micropylar end. These
are together called the filiform apparatus. It helps in leading the
pollen tubes into the synergids.
Three cells are at the chalazal end, and are called antipodal cells.
A typical angiosperm female gametophyte is 7-celled and 8-nucleated
at maturity.
Pollination
It is the process of transfer of pollen grains from the anther to the
stigma.
Depending on the source of pollen, pollination can be divided as
follows:
o Autogamy It is the transfer of pollen grains from the anther to
the stigma of the same flower. Autogamy requires the anther
and the stigma to lie close. It also requires synchrony in the
pollen release and stigma receptivity.
Plants like Viola, Oxalis, etc., produce two kinds of flowers
chasmogamous flowers (with exposed anther and stigma)
and cleistogamous flowers (which do not open at all and only
autogamy occurs).
o Geitonogamy It is the transfer of pollens from the anther of
one flower to the stigma of another flower in the same plant.
Genetically, it is similar to autogamy, but it requires pollinating
agents.
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Post-Fertilisation Events
It includes development of endosperm and embryo, and maturation of
ovules into seeds and ovaries into fruits.
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Formation of Endosperm
The endosperm develops before the embryo because the cells of the
endosperm provide nutrition to the developing embryo.
The primary endosperm nucleus repeatedly divides to give rise to
free nuclei. This stage of development is called free nuclear
endosperm.
Cell wall formation occurs next, resulting in a cellular endosperm.
The endosperm may be either fully consumed by the growing embryo
(as in pea and beans) or retained in the mature seed (as in coconut
and castor).
Development of Embryo
The embryo develops at the micropylar end of the embryo sac where
the zygote is situated.
The zygote gives rise first to the pro-embryo, and then to the globular,
heart-shaped, mature embryo.
A typical dicot embryo consists of an embryonal axis and two
cotyledons.
The portion of the embryonal axis above the level of cotyledons is
called epicotyl. It contains the plumule (shoot tip). The portion below
the axis is called hypocotyl. It contains the radicle (root tip). The root
tip is covered by the root cap.
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In some plants, floral parts other than the ovary take part in fruit
formation, as in apple and strawberry. In these, the thalamus
contributes to fruit formation. Such fruits are called false fruits. Fruits
that develop from the ovary are called true fruits.
Some fruits develop without fertilisation, and are known as
parthenocarpic fruits (example: banana).
Apomixis and Polyembryony
Some plants produce seeds without fertilisation. This process of seed
formation is known as apomixis.
Apomixis is a form of asexual reproduction mimicking sexual
reproduction.
In some species, apomixis occurs as the diploid egg cell is formed
without meiosis, and develops into embryo without fertilisation.
In some varieties of citrus and mango, the nucellus cells divide and
protrude into the embryo sac to develop into embryos. In such cases,
each ovule may contain several embryos and this condition is called
polyembryony.
Apomixis is important for producing hybrid varieties of fruits and
vegetables, and also for increasing crop yield multifold.
Human Reproduction
Male and Female Reproductive Systems
Human beings reproduce sexually and are viviparous.
In humans, the reproductive phase starts after puberty.
It involves:
o Gametogenesis
o Insemination
o Fertilisation
o Implantation
o Gestation
o Parturition
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The seminiferous tubules open into the vasa efferentia through the
rete testis.
The vasa efferentia open into the epididymis, which leads to the vas
deferens. The vas deferens opens into the urethra along with a duct
from the seminal vesicle called the ejaculatory duct.
The ejaculatory duct stores the sperms and transports them to the
outside
The urethra starts from the urinary bladder, extends through the penis
and opens via the urethral meatus.
Accessory glands include:
o A pair of seminal vesicles
o Prostate gland
o A pair of bulbourethral glands
The secretions of these glands make up the seminal plasma, and
provide nutrition and a medium of motility to the sperms.
The Female Reproductive System
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Ovaries
They are the primary female sex organs. They produce the ovum and
other ovarian hormones.
They are located in the lower abdomen, and are 2 to 4 cm in length.
They are connected by ligaments to the pelvic walls and to the
uterus.
Each ovary is covered by epithelium, and contains the ovarian
stroma.
The ovarian stroma is made up of:
o Peripheral cortex
o Inner medulla
Oviducts
They are also called fallopian tubes.
They are 10 to 12 cm long, and extend from the ovary to the uterus.
The part of each oviduct lying towards the ovary is funnel shaped,
and is called infundibulum. It has finger-like projections called
fimbriae.
The infundibulum leads to the ampulla, and then to the isthmus,
which has a narrow lumen opening into the uterus.
Uterus
It is also called womb, and is pear shaped.
It is connected to the pelvic walls by ligaments.
The uterine wall consists of:
o External perimetrium
o Middle myometrium
o Internal endometrium, which lines the uterine cavity
The endometrium undergoes changes during the menstrual cycle.
Cervix and Vagina
The cervix connects the uterus to the vagina.
The cervix and the vagina constitute the birth canal.
External Genitalia
Consists of:
o Mons pubis Fatty tissue covered by skin and pubic hair
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o Neck
o Middle piece
o Tail
The whole sperm is enclosed in a plasma membrane.
The head consists of a haploid nucleus and a cap-like acrosome,
which contains enzymes that aid in fertilisation.
The middle piece contains several mitochondria, which produce
energy for the motility of the sperm.
Sperms released by the seminiferous tubules are transported by the
accessory ducts.
Secretions of epididymis, vas deferens, seminal vesicles, and
prostate are essential for maturation and motility of sperms.
Oogenesis
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The LH and FSH are at their peak in the middle of the cycle (14 th
day), and cause the rupture of the Graffian follicles to release ovum.
This phase is called the ovulatory phase.
The remains of the Graffian follicles get converted into the corpus
luteum, which secretes progesterone for the maintenance of the
endometrium.
In the absence of fertilisation, the corpus luteum degenerates,
thereby causing the disintegration of the endometrium and the start of
a new cycle.
In humans, the menstrual cycle ceases to operate at the age of 50
years. This phase is known as the menopause.
Fertilisation and Implantation
During coitus, the semen is released into the vagina, passes through
the cervix of the uterus and reaches the ampullary-isthmic junction of
the fallopian tube.
The ovum is also released into the junction for fertilisation to occur.
The process of fusion of the sperm and the ovum is known as
fertilisation.
During fertilisation, the sperm induces changes in the zona pellucida
and blocks the entry of other sperms. This ensures that only one
sperm fertilises an ovum.
The enzymatic secretions of the acrosomes help the sperm enter the
cytoplasm of the ovum.
This causes the completion of meiotic division of the secondary
oocyte, resulting in the formation of a haploid ovum (ootid) and a
secondary polar body.
Then, the haploid sperm nucleus fuses with the haploid nucleus of
the ovum to form a diploid zygote.
Mitosis starts as the zygote moves through the isthmus of the oviduct
(cleavage) and forms 2, 4, 8, 16 daughter cells called blastomeres.
The 816 cell embryo is called a morula, which continues to divide to
form the blastocyst. The morula moves further into the uterus.
The cells in the blastocyst are arranged into an outer trophoblast
and an inner cell mass.
The trophoblast gets attached to the uterine endometrium, and the
process is called implantation. This leads to pregnancy.
The inner cell mass gets differentiated to form the embryo.
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Reproductive Health
Reproductive Health
It is defined as the total well being in all aspects of reproduction.
India as a country ensures reproductive health to all, and since 1951,
successive governments have introduced a number of programmes
to ensure this, e.g., family planning and RCH (Reproductive and
Child Healthcare) programmes.
The aim of these programmes is to create awareness among people
about the various aspects of reproductive health.
Several audio-visual aids and pamphlets have been developed by
both governmental and non-governmental organisations.
Awareness is being created among school children by introducing
sex education in schools.
It is essential to provide medical assistance to people with problems
related to STDs, pregnancy, contraception and infertility, especially in
villages.
Amniocentesis (foetal sex determination based on chromosomal
pattern) has been banned to check female foeticide.
Population Explosion
Improvement in the quality of life due to all-round development (better
health facilities and improved conditions of living) has lead to a large
increase in the world population (around 1 billion in the year 2000).
The causes of population explosion are:
o Decline in death rate (including maternal mortality rate and
infant mortality rate)
o Increase in the number of people in the reproducible age
Population explosion causes a scarcity of every basic need.
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PRINCIPAL OF INHERITANCE
Genetics
Genetics is a branch of biology dealing with inheritance and variation
of characters from parents of offspring.
Inheritance
Process by which characters are passed on from parent to progeny
Variation
Degree by which the progeny differs from its parents
Mendels Experiments
Gregor Johann Mendel known as the father of genetics proposed the
laws of inheritance.
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S. No.
Character
Dominant
Recessive
Stem height
Tall
Dwarf
Flower colour
Violet
White
Flower position
Axial
Terminal
Pod shape
Inflated
Constricted
Pod colour
Green
Yellow
Seed shape
Round
Wrinkled
Seed colour
Yellow
Green
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Law of Segregation
This law states that the two alleles of a pair segregate or separate
during gamete formation such that a gamete receives only one of the
two factors.
In homozygous parents, all gametes produced are similar; while in
heterozygous parents, two kinds of gametes are produced in equal
proportions.
Incomplete Dominance
In incomplete dominance, F1 generation has a phenotype that does
not resemble either of the two parents, but is a mixture of the two.
Example Flower colour in dog flower (snapdragon), where:
o RR Red flowers
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o rr White flowers
o Rr Pink flowers
Here, genotypic ratio remains same as in Mendelian crosses, but
phenotypic ratio changes since complete dominance is not shown by
R (hence, incomplete dominance).
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The altered version of the gene must not perform the functions that a
normal gene performs. It must affect the phenotype.
The original gene is said to be dominant while the modified gene is
recessive.
Law of Segregation and Co-dominance
Co-dominance
In co-dominance, the F1 progeny resembles both the parents.
Example: ABO blood groups in human beings
ABO blood groups are controlled by gene I. Gene I has three alleles,
I A, I B and i. A person possesses any two of the three alleles.
IA and I B dominate over i. But with each other, IA and IB are codominant.
IA and I B contain A and B types of sugar, while i does not contain any
sugar.
Allele from
Parent 1
Allele from
Parent 2
Genotype of
offspring
Blood type of
offspring
IA
IA
IAIA
IA
IB
IAIB
AB
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IA
IAi
IB
IA
IAIB
AB
IB
IB
IBIB
IB
IBi
ii
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44
45
46
Mutation
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Chromosomal Disorders
Total number of chromosomes in humans = 46 (23 pairs)
Total 23 pairs = Autosomes (22 pairs) + Sex chromosomes (1 pair)
Monosomy Lack of any one pair of chromosomes
Trisomy Inclusion of an additional copy of chromosome
Aneuploidy Loss or gain of chromosomes due to the failure of
segregation of chromatids during cell division
Downs Syndrome
o Cause: Presence of an additional copy of chromosome 21
(Trisomy of 21)
o Affected individual has short stature, small, round head,
furrowed tongue, partially opened mouth, palm crease,
congenital heart disease and mental retardation.
Klinefelter Syndrome
o Cause: Additional copy of X chromosome, i.e., 47
chromosomes (XXY)
o Affected individual has an overall masculine development with
gynaecomastia; individual is sterile
Turners Syndrome
o Cause: Absence of one X chromosome, i.e., 45 chromosomes
(XO).Affected females are sterile; have rudimentary ovaries;
secondary sexual characters are absent
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to each other.
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o The plane of one base pair stacks over the other in a double
helix. This provides stability to the helix along with hydrogen
bonding.
Packaging of DNA Helix
Packaging of DNA Helix
Distance between two consecutive base pairs in a DNA = 0.34 nm =
0.34 109 m
Total number of base pairs in a human DNA = 6.6 10 9 bp
Total length of human DNA = 0.34 109 6.6 109
= ~ 2.2 m
2.2 m is too large to be accommodated in the nucleus (10 6 m).
Organisation of DNA in prokaryotes:
o They do not have nucleus. DNA is scattered.
o In certain regions called nucleoids, DNA (negatively charged) is
organised in large loops and is held by some proteins
(positively charged).
Organisation of DNA in eukaryotes:
o They have positively charged basic proteins called histones
(positive and basic due to presence of positive and basic amino
acid residues, lysine and arginine).
o Histone octamer Unit of eight molecules of histone
o DNA (negatively charged) winds around histone octamer
(positively charged) to form nucleosome.
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S strain Bacteria
R strain Bacteria
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Griffiths experiment
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material.
DNA
RNA
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o Has deoxyribose
sugar
o 5-methyl uracil
(thymine) is present.
o DNA is stable.
o Chemically less
reactive, mutates
slowly
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DNA extracted after 40 minutes had equal amount of hybrid and light
intensities.
This implies that the newly synthesised DNA obtained one of its
strands from the parent. Thus, replication is semi-conservative.
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'.
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Gene
The DNA sequence which codes for tRNA or rRNA molecule defines
a gene.
Cistron Segment of DNA that contains the genetic code for a single
polypeptide
The structural genes could be of two types:
o Monocistronic (mostly in eukaryotes)
o Polycistronic (mostly in prokaryotes)
Monocistronic genes have two parts:
o Exon Sequences that code for a particular character and is
expressed in a matured and processed mRNA
o Intron Interrupting sequences that do not appear in a mature
and processed mRNA
Regulatory genes Sequences that do not code for anything, but
have regulatory functions
Types of RNA & Transcription Process
Types of RNA
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o terminated.
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tRNA
tRNA is an adapter molecule. On one hand, it reads the genetic code
and on the other hand, it binds to specific amino acids.
tRNA has an anticodon loop that has bases complementary to the
mRNA code and an amino acid acceptor end where it binds to the
corresponding amino acid.
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Translation
The mRNA contains the genetic information, which is translated into
the amino acid sequence with help of tRNA. Amino acids are
polymerised to form a polypeptide.
Amino acids are joined by peptide bond.
First of all, charging of tRNA (amino-acylation of tRNA) takes place.
In this, amino acids are activated in the presence of ATP and are
linked to their corresponding tRNA.
Ribosomes are the workbenches for translation. Ribosomes have 2
subunits: a large subunit and a small subunit.
Smaller subunit comes in contact with mRNA to initiate the process of
translation.
Translational unit in an mRNA is the region flanked by start codon
and stop codon.
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Untranslated regions (UTR) are the regions on mRNA that are not
themselves translated, but are required for efficient translation
process. They may be present before start codon (5 UTR) or after
stop codon (3 UTR).
Initiator tRNA recognises the start codon. (Initiation)
Then t-RNA-amino acid complexes bind to their corresponding codon
on the mRNA and base pairing occurs between codon on mRNA and
tRNA anticodon.
tRNA moves from codon to codon on the mRNA and amino acids are
added one by one. (Elongation)
Release factor binds to stop codon to terminate the translation.
(Termination)
Regulation of Gene Expression
Regulation of gene expression could be exerted at following levels.
o Transcriptional level (following of primary transcripts)
o Processing level (splicing)
o Transport of mRNA from nucleus to cytoplasm
o Translational level
In addition, metabolic, physiological, or environmental conditions
regulate the expression of genes.
Expression of genes coding for enzymes is required only when
substrate for that enzyme is available.
For example:
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Lactose
Glucose + Galactose
E.coli synthesises beta-galactosidase, only when lactose is available.
Regulation in prokaryotes
o Gene expression is regulated by controlling the rate of
transcriptional initiation.
o The activity of RNA polymerase at a given promoter is
regulated by accessory proteins. The accessory proteins affect
the ability of a promoter to recognise start sites.
o A regulatory protein could be activator or repressor.
o Accessibility of promoter is also affected by operators. Operator
is the region located adjacent to promoter.
o Each operon has a specific operator and a specific repressor.
o Usually operator binds to a repressor protein.
Regulation of Lac Operon
Lac Operon
Operon An arrangement where a polycistronic gene is regulated by
a common promoter and regulatory genes
Lac operon, trp operon, his operon, val operon are the examples of
such systems.
The elucidation of lac operon as a transcriptionally active system was
first done by geneticist Jacob and biochemist Monod.
Genes constituting lac operon:
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Gene
Nature
Function
i gene
Inhibitor
z
gene
Structura
l
Lactose
Galactose + Glucose
y
gene
Structura
l
a
gene
Structura
l
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ESTs As the name suggests, this refers to the part of DNA that is
expressed, i.e. transcribed, as mRNA and translated into proteins
thereafter. It basically focuses on sequencing the part denoting a
gene.
Annotation In this approach, entire genome (coding + non-coding)
is sequenced and later on function is assigned to each region in the
genome.
Genome Sequencing
DNA from the cells is isolated and is randomly broken into fragments
of smaller sizes.
These fragments are cloned into suitable host using vectors.
Cloned fragments amplify in the host. Amplification facilitates an easy
sequencing.
Common vectors used BAC (Bacterial artificial chromosomes) and
YAC (Yeast artificial chromosomes)
Common hosts Bacteria and yeasts
Automated sequencers are used to sequence these smaller
fragments (Sanger sequencing).
The sequences so obtained are arranged based on overlapping
regions within them (alignment).
Alignment of the sequences is also done automatically by computer
programs.
Then these sequences are annotated and assigned to each
chromosome.
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DNA Fingerprinting
Introduction
DNA fingerprinting is a method for comparing the DNA sequences of
any two individuals.
99.9% of the base sequences in all human beings are identical. It is
the remaining 0.1% that makes every individual unique.
It is a really difficult and time-consuming task to sequence and
compare all 3 109 bases in two individuals. So, instead of
considering the entire genome, certain specific regions called
repetitive DNA sequences are used for comparative study.
Basis of DNA Fingerprinting
Repetitive DNA is separated from bulk genomic DNA since it appears
as a distinct peak during density gradient centrifugation.
Major peak: Formed by bulk DNA
Smaller peak: Satellite DNA
Satellites are of two typesmicro-satellites and mini satellites,
depending upon the base composition, length of segment and the
number of repetitive units.
Satellites do not code for proteins, but have a major role to play in
DNA fingerprinting.
Polymorphism is actually a result of mutation. A germ cell mutation
(which can pass on to the next generation through sexual
reproduction) gives rise to polymorphism in populations.
In other words, an inheritable mutation if observed in higher
frequencies in a population is known as polymorphism.
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Evolution
Origin of Life
Year
Scientist
Theory/Experiment
Conclusion
1927
Lemaitre
The universe
expanded from
explosion of a
primordial, hot
substance.
1924
1929
Oparin
and
Haldane
Simple organic
molecules originated
from inorganic
precursors.
1952
Stanley
Miller and
Urey
Synthesis of biomolecules by
creation of similar conditions
as primitive atmosphere on
laboratory scale
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Adaptive Radiation
During his exploration of the Galapagos Islands, Darwin noticed that
there were many varieties of finches in the same island.
They varied from normal seed eating varieties to those that ate
insects.
This process of evolution starting from a single point and radiating in
different directions is called adaptive radiation.
The other example for this is the evolution of the Australian
marsupials from a single ancestor. Placental mammals also exhibit
similarities to their corresponding marsupial. Example: placental wolf
and the Tasmanian wolf
When more than one adaptive radiation occurs in an isolated
geographical area, the phenomenon is called convergent evolution.
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Darwin did not quite explain how evolution gave rise to different
species of the same organism.
Mendel mentioned about inheritable factors, which influenced the
phenotype of an organism.
Hugo de Vries based on his work on evening primrose suggested that
variations occurred due to mutations.
Mutations are random and directionless while the variations that
Darwin talked about were small and directional. Hugo de Vries gave
the name saltation (single step large mutation) to the mutations
which brought about speciation.
Hardy-Weinberg Principle
The frequency of occurrence of alleles of a gene in a population
remains constant through generations unless disturbances such as
mutations, non-random mating, etc. are introduced.
Genetic equilibrium (gene pool remains constant) is a state which
provides a baseline to measure genetic change.
Sum total of all allelic frequencies is 1.
Individual frequencies are represented as p and q such as in a
diploid, where p and q represent the frequency of allele A and a.
The frequency of AA is p2, that of aa is q2, and that of Aa is 2pq.
Hence, p2 + 2pq + q2 = 1, which is the expansion of (p + q)2.
When the frequency measured is different from that expected, it is
indicative of evolutionary change.
Hardy-Weinberg equilibrium is affected by
gene flow or gene migration
genetic drift (changes occurring by chance)
mutation
genetic recombination
natural selection
Sometimes, the change in allele frequency is so prominent in the new
sample of population that they become a different species and the
original drifted population becomes the founder. This effect is called
founder effect.
The advantageous mutations that help in natural selection over the
generations give rise to new phenotypes and result in speciation.
Evolution of Plants and Animals
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Evolution of Plants
Cellular life forms occurred on earth about 2000 million years ago.
Some of these cells had the ability to produce oxygen through
reactions similar to photosynthesis.
Slowly, single-celled organisms became multicellular.
Seaweeds and some plants probably existed around 320 million
years ago.
Evolution of Animals
Animals evolved about 500 million years ago. The first of them to
evolve were invertebrates.
Jawless fishes evolved around 350 million years ago.
Some of the fishes could go on land, and then come back to water.
These were the first amphibians. In 1938, a fish Coelacanth, which
was thought to be extinct, was caught in South Africa. This variety of
fish, called lobefins, is believed to have evolved into the first
amphibians.
Amphibians evolved into reptiles. In the next 200 million years,
reptiles of different sizes dominated the earth. However, about 65
million years ago, some of them such as dinosaurs disappeared.
The first among the mammals were small shrew-like mammals.
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Evolution
Characteristics
15 million
years ago
34
million
years ago
Man-like primates
2 million
years ago
Australopithecines, also
called Homo habilis,lived in
East Africa
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Homo erectus
1,000 40,
000 years
ago
Neanderthal man
75, 000
10, 000
years ago
Homo sapiens
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Elephantiasis (filariasis)
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Immunity
What is immunity?
The ability of body to fight the disease-causing organisms is called
immunity.
Types of immunity
Immunity is of two types innate immunity and acquired immunity.
Innate immunity It is present from the time of birth. It is nonspecific. It consists of 4 kinds of barriers.
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Now when the pathogen again attacks the person, memory B and Tcells generate a massive immune response and the pathogen is
killed.
Problems of immune system
Allergies
o Exaggerated immune response to certain antigens present in
environment
o Allergens Substances in response to which allergy is
produced
E.g., dust, pollen, etc.
o Antibodies involved IgE type
o During allergic reactions, chemicals such as histamines and
serotonins are released.
o Symptoms Sneezing, watery eyes, difficulty in breathing, etc.
o Allergy test Patient is injected with small doses of allergens to
monitor his response.
o Antihistamines, adrenalins, and steroids may be given so that
the symptoms of allergy subside.
Autoimmunity
o In autoimmunity, body generates immune response against its
own cells.
o Reasons Genetic and other unknown reasons
o Example Rheumatoid arthritis is an autoimmune disease.
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Cocaine
Source: Coca plant Erythroxylum coca, found in South America
Consumed by: Snorting
Mode of action: Interference with transfer of neurotransmitter,
dopamine
Effect: Stimulates the CNS, producing a sense of euphoria and
increased energy; excessive dosages cause hallucination
Drugs Normally Used as Medicines
Drugs like barbiturates, amphetamines, benzodiazepines, LSD
(Lysergic acid diethyl amides) are used as medicines to help patients
with mental illness and insomnia.
Morphine: It is a pain killer which is used for patients who have
undergone surgery, but it is also abused.
Nicotine
Present in tobacco, which is smoked, chewed or snuffed
Mode of action: Stimulates the adrenal gland to release adrenaline
and nor-adrenaline
Effect: Increases blood pressure and heart rate
Ill Effects of Smoking
Increased risk of diseases like bronchitis, emphysema, coronary
heart disease, gastric ulcer and cancer (throat, lung and urinary
bladder)
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Farm Management
Dairy Farm Management
o Milk yield is dependent upon the quality of breed selected.
Quality encompasses yielding potential and disease resistance.
o Care of cattle Proper accommodation, adequate water,
feeding in a scientific manner (quality of fodder), hygiene, visits
by a veterinary doctor
o All these processes nowadays have become mechanised and
proper record keeping is followed.
Dairy Farm Management
o Poultry includes meat from birds such as chicken, ducks, and
turkey.
o The main emphasis in poultry farming is selection of a diseasefree and healthy breed.
o Safe farm conditions, proper feed, water, and hygiene are also
necessary.
Animal Breeding
Breed A group of animals related by descent and similar in most
characters such as general appearance, features, size, etc.
Aims of breeding:
o To increase yield of animals
o To improve desirable qualities in produce
Breeding is of two types inbreeding and out-breeding.
Inbreeding
o Mating of more closely related individuals of same breed for
four generations
o Superior females and superior males are identified and mated.
o Superior females Produce more milk per lactation
o Superior males Give rise to a superior progeny
o Inbreeding increases homozygosity. It evolves a pure line.
o It accumulates superior genes, but also threatens to
accumulate harmful recessive genes
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o Pathogen resistance
o Tolerance to insects and pests
Steps Involved in Breeding a New Genetic Variety of a Crop
Collection of genetic variability
o Genetic variability is availed from the wild relatives of the crop.
o Hence, all the wild varieties and relatives of the crop are
collected and preserved.
o The natural genes available in a population are utilised by this
method.
o Entire collection of plants/seeds (wild types/relatives) of the
given crop, which has all the diverse alleles for all genes, is
called germplasm collection.
Evaluation and selection of parents
o From the available genetic variability, the germplasm is
analysed and evaluated to identify the plants with desirable
traits.
Crop hybridisation among selected parents
o Two selected parents are crossed (hybridised). This facilitates
the combination of desired traits from two different plants.
o Pollen grains from one plant are dusted over the stigma of the
other plant.
Selection of superior recombinants
o Among the hybrid progeny, those plants are selected which
have the desired character combination.
o Careful scientific evaluation of progeny is required for selection.
o This step yields the plant that is superior to both the parents.
Testing, release and commercialisation
o Selected yields are evaluated for traits like quality, disease
resistance, insect resistance, etc.
o These crops are grown in research fields and their performance
is recorded under ideal conditions.
o This crop is then grown by farmers at several locations, for at
least three growing seasons.
o The crop is evaluated by comparing with the best available
local crop cultivar (which acts as a reference).
Indian Hybrid Crops
Wheat and Rice
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Substance produced
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Fungus Aspergillus
niger
Citric acid
Bacterium
Acetobacter aceti
Acetic acid
Bacterium
Clostridium butylicum
Butyric acid
Bacterium
Lactobacillus
Lactic acid
Yeast S.cerevisiae
Ethanol
Bacterium
Streptococcus
Fungus Trichoderma
polysporum
Yeast Monascus
purpureus
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The introduced piece of DNA does not replicate in the host unless it is
integrated with the chromosome of host.
For getting replicated, the foreign DNA must integrate into the host
DNA sequence having origin of replication. When this integration
occurs, foreign DNA is replicated and many copies are formed. This
process is called cloning (the process of formation of multiple
identical copies of DNA).
Construction of a Recombinant DNA
Plasmid (autonomously replicating, circular, extra-chromosomal DNA)
is isolated.
Plasmid DNA acts asa vector since it is used to transfer the piece of
DNA attached to it to the host.
Plasmid DNA also contains genes responsible for providing antibiotic
resistance to the bacteria.
Plasmid DNA was cut with a specific restriction enzyme (molecular
scissors that cut a DNA at specific locations).
The DNA of interest (to be inserted) was also cut with the same
restriction enzyme.
The DNA of interest is hybridised with the plasmid with the help of
DNA ligase to form a Recombinant DNA.
Recombinant DNA is then transferred to a host such as E.coli, where
it replicates by using the hosts replicating machinery.
When E.coli is cultured in a medium containing antibiotic, only cells
containing recombinant DNA will be able to survive due to antibiotic
resistance genes and one will be able to isolate the recombinants.
Restriction Enzymes as Tools of RDT
Restriction enzymes are specialised enzymes that recognise and cut
a particular sequence of DNA.
Nucleases are of two types:
o Endonucleases Cut the DNA at specific positions within the
DNA
o Exonucleases Cut the DNA at the ends (Remove the
nucleotides at the ends of the DNA)
Every restriction enzyme identifies different sequences (Recognition
sequences). Over 900 restriction enzymes have been isolated, all of
which recognise different sequences.
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These bands are cut from the agarose gel and extracted from the gel
piece (elution).
DNA fragments are purified and these purified DNA fragments are
used in constructing recombinant DNAs.
Cloning vectors & host as tools of RDT
Cloning Vectors
Plasmids and bacteriophages are commonly used as cloning vectors.
Both of these have the ability to replicate within the bacterial cells
independent of the chromosomal DNA.
Bacteriophages Have high copy number (of genome) within the
bacterial cell
Plasmids May have 1 2 copy number to 15 100 copy number
per cell
If foreign DNA is linked to these vectors, then it is multiplied to the
number equal to the copy number of vector.
Features present in the vector itself help in the easy isolation of
recombinants from the non-recombinants.
Components of a plasmid cloning vector
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Ecosystem
Ecosystem is the interaction of living things among themselves and
with their surrounding environment.
There are two basic ecosystems terrestrial and aquatic.
Structure of Ecosystem
The interactions between the various biotic and abiotic factors of an
ecosystem lead to the maintenance of the ecosystem.
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o Pyramid of biomass
o Pyramid of energy
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Nutrients are never lost from the ecosystem. They are only recycled
from one state to another.
The movement of nutrients through the various components of the
ecosystem is called nutrient cycling or biogeochemical cycles. They
are of two types:
o Gaseous Reservoir for these types of cycles exist in the
atmosphere.
o Sedimentary Reservoir for these types of cycles exist in the
earths crust.
Carbon Cycle
About 49% of the dry weight of living organisms is made up of
carbon.
The ocean reserves and fossil fuels regulate the amount of CO 2 in the
atmosphere.
Plants absorb CO2 from the atmosphere for photosynthesis, of which
a certain amount is released back through respiratory activities.
A major amount of CO2 is contributed by the decomposers who
contribute to the CO2 pool by processing dead and decaying matter.
The amount of CO2 in the atmosphere has been increased
considerably by human activities such as burning of fossil fuels,
deforestation.
Phosphorus Cycle
Phosphorus is an important constituent of cell membranes, nucleic
acids, and cellular energy transfer systems.
Rocks contain phosphorus in the form of phosphate.
When rocks are weathered, some of the phosphate gets dissolved in
the soil solution and is absorbed by plants.
The consumers get their phosphorus from the plants.
Phosphorus returns back to the soil by the action of phosphatesolubilising bacteria on dead organisms.
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Environmental Issues
Pollution is the undesirable change brought about by chemical, particulate
matter, or biological materials to air, water, or soil.
Air Pollution
Air is a complex, dynamic natural entity, which is essential for
supporting life on earth.
Air pollutant is a substance that causes harm to the humans and
other living organisms.
Some of the common pollutants of air:
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o Nitrogen dioxide
o Sulphur dioxide
o Carbon monoxide and carbon dioxide
o Volatile organic compounds
o Particulate matter
Control of Air Pollution
Air pollution causes severe respiratory disorders in humans and other
animals and also affects plants. It can be controlled by the following
ways:
o Fitting smokestacks and smelters, with filters to separate
pollutants from the harmless gases
o Particulate matter can be removed by using an electrostatic
precipitator. It contains electrode wires maintained at several
thousand volts, which produce electrons. These electrons cling
on to dust particles and give them a net negative charge and
are attracted by collecting plates, which are grounded. The
velocity of air passing through the plates should be low enough
to allow the dust to fall.
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