KURSUS :

SUL1015
INDUSTRIAL TRANING

INTERNSHIP REPORT

DISEDIAKAN OLEH :
NAMA PELAJAR

NO. MATRIK

MOHD ADAM BIN YEGGE

E20122005498
PROGRAM :

A2003-DIPLOMA TEKNOLOGI MAKMAL

PENYELIA:
PROF. MADYA. DR. YAYA RUKAYADI
PENSYARAH
LABORATORI HASILAN SEMULA JADI
INSTITUT BIOSAINS
UNIVERSITI PUTRA MALAYSIA

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CONTENT
CHAPTER 1: ACKNOWLEGMENT

CHAPTER 2: INTRODUCTION

CHAPTER 3: BACKGROUND OF INDUSTRY

4-6

CHAPTER 4: INTERNSHIP SUMMARY

CHAPTER 5: INDUSTRIAL TRAINING

8-11

5.1 Project
5.2 Introduction of Project
5.3 Materials and Method
5.3.1 Chemical Reagent
5.3.2 Equipments and Tools
5.3.3 Method
5.4 Result
CHAPTER 6: SUGGESTIONS

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CHAPTER 7: CONCLUSION

12

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CHAPTER 1: ACKNOWLEGMENT
I would like to express my greatest thanks and appreciation to my research supervisor Assoc.
Prof. Dr. Yaya Rukayadi for his invaluable guidance, understanding, patience and constant
encouragement throughout the course of my study.
Special thanks to IBS students, the staff and lab assistants at Institute of Bioscience, UPM
who gave me support and ensure that my laboratory lab work done under good and safe
conditions.
Last but not least, I am greatly indebted to my beloved family for their wise guidance,
endurance, understanding, encouragement and support throughout my study life also not be
forgotten my internship mate, Amir Hamzah.

CHAPTER 2: INTRODUCTION
Most of the third year students are taking the SUL1015. The purpose of taking the subject of
Industrial Training, SUL1015 is to expose students with the experience of working. Besides,
student can apply what we have learnt in class during the internship. Other than that, we can
learn something new and grab more knowledge in an industrial work.

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CHAPTER 3: BACKGROUND OF INDUSTRY

The Institute of Bioscience (IBS), Universiti Putra Malaysia was established on 1st of August
1996 and was the first research institute founded in UPM. IBS was established to boost
Research and Development (R&D) and postgraduate training in various fields of biological
sciences. The emphasis on research was given to strengthen the scientific and technological
capabilities of the country as well as to develop relevant human capital to support the
Industrial and Agricultural Master Plan. Since its inception, IBS areas of research have
grown and it is gradually developing into a leading research centre in UPM.
Institute of Bioscience is recognized as a Higher Institution Centre of Excellence (HICoE). It
was announced by YAB Dato' Sri Mohd. Najib Tun Haji Abdul Razak, Malaysia Prime
Minister during the launching of the National Professors Association (NPA) and the Higher
Education Centre of Excellence (HICoE) on 1st April 2010 at Putrajaya International
Convention Center (PICC).
On 9 February, 1995, the Senate, supported the idea of establishing Universiti Pertanian
Malaysia Research Institute with the aim that it will organise research and development
programmes in line with the corporate plan of Universiti Pertanian Malaysia. The proposed
Institute was conceived as a central research centre in UPM for researchers from various
faculties and centres to conduct strategic and multidisciplinary research in a conducive and
world class research environment.
The proposal on the establishment of the Institute was presented to the Universiti Pertanian
Malaysia Board of Directors on 1 April, 1995 which agreed in principle to the proposal and
requested a detailed proposal paper. The name Universiti Pertanian Malaysia Research
Institute was revised and the new name, Institute of Bioscience (IBS), was accepted by the

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Senate. In its 120 th Meeting on 9 September, 1995, the Board of Directors agreed to the
formation of the Institute.
The Committee on Education Planning under the Ministry of Education Malaysia in its 131 st
Meeting held on 27 April, 1996, approved the proposal for the establishment of Institute of
Bioscience, UPM.
Following that, the then Vice Chancellor of Universiti Pertanian Malaysia, Y. Bhg. Prof. Tan
Sri Dr. Syed Jalaluddin Syed Salim announced the establishment of the Institute of
Bioscience and appointed the Director and Deputy Director to head and develop the Institute.
Prof. Dr. Abdul Rani Bahaman had been appointed as the first Director of IBS and Prof. Dr.
Ho Yin Wan as the Deputy Director.
Over the past 17 years, the Institute of Bioscience has rearranged and developed the existing
units into 5 laboratories to ensure the smoothness and effectiveness of administration as well
as resource and facilities management. Each laboratory has 2 main functions, i.e. doing
researches and provides the Service and Facility Unit. These 5 laboratories are:

Laboratory of Natural Products

Laboratory of Vaccines and Immunotherapeutics

Laboratory of Molecular Biomedicine

Laboratory of Biotechnology Marine

Laboratory of Cancer Research UPM-MAKNA

Each research project is headed by Associate Researcher invited as integrated Institute

researchers and are assisted by Science Officers, Research Officer, Laboratory Assistants and

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other supporting staffs. Research programs conducted at the Institute of Bioscience are
potentially competitive to generate knowledge in biology science by focusing on integrated
and multidiscipline research involving the faculties in UPM.
Human resource development in IBS continued to expand in line with the development of its
infrastructure and facilities to accommodate the needs of its staff when conducting research.
Presently, IBS has 119 permanent and contract staff consisting of 50 Officers from the
Professional and Management Group and 69 supporting staff under its administration.

Institute of Bioscience (IBS)

Laboratory of Natural Products (LHS)

Division of Microbial Laboratory

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CHAPTER 4: INTERNSHIP SUMMARY

The diploma internship program, SUL1015 supports students from Faculty of Sciences in
UPSI to pursue careers in chemistry industry and research. Through this program,
undergraduate students from Department of Chemistry are introduced and briefed to the field
of this internship with Assoc. Prof. Dr. Saripah Salbiah Syed Abdul Aziz as the coordinator.
Students are required to work for 10 weeks during the semester start from 8th of June 2014
until 21st of August 2014. The internship may include laboratory experiments, and data
collection. The internship students were placed at the Natural Product Laboratory, Institute Of
Bioscience, Universiti Putra Malaysia.. I was placed at LHS division of Microbial Laboratory
under supervision Assoc. Prof. Dr. Yaya Rukayadi. The title of my project is Determination
of percentages yield of several medicinal plants using ethanol absolute as a solvent.

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CHAPTER 5: INDUSTRIAL TRAINING

5.1 Project
I get the project of determine the percentages of yield of several medicinal plants
using Ethanol absolute as a solvent.
5.2 Introduction of Project
Rotary evaporators (also called "rotavaps") are used to remove solvents from reaction
mixtures and can accommodate volumes as large as 3 litres. They are found in almost
every organic laboratory, since they allow performing this task very quickly. A typical
rotary evaporator has a water bath that can be heated in either a metal container or
crystallization dish. This keeps the solvent from freezing during the evaporation
process. The solvent is removed under vacuum, is trapped by a condenser and is
collected for easy reuse or disposal. Most labs use a simple water aspirator vacuum on
their rotavaps, so a rotavap cannot be used for air and water-sensitive materials unless
special precautions are taken i.e. additional traps are used.
Since the flask is rotated during the evaporation process, the surface area is larger than
normal which increases the evaporation rate significantly. The solvent is collected in a
flask and can properly be disposed off afterwards (organic solvent waste). In addition,
this method also avoids overheating of the target compound i.e. oxidation because
lower temperatures are used. The same rules like for vacuum filtrations apply here in
terms of the glassware and other precautions i.e no cracks on the flask, etc.

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5.3 Material and Method

5.3.1 Chemical Reagent
Chemical reagent used in this study are Ethanol absolute 100% (v v).
5.3.2 Equipment and Tools
Instrument used in this study were rotary vacuum evaporator, incubator, and
analytical balance. Tools used in this study included schott glass bottles,
graduated cylinder, filter funnel, bchner flask, Bchner funnel, rubber bung,
and round bottom flask.

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5.3.3 Method
Extraction
Dried plants or seeds were ground in 100gram. The sample was soaked for 4
days at room temperature by using 400mL of 100% (v/v) Ethanol. Filter the
plants or seeds extracts using Whatman filter paper. Collect the filtrate. After
filter, the remaining extracts were dissolve again in 400mL of solvent for
second times. The filtration were concentrated with a rotary vacuum
evaporator at 50 and dried below 60C in oven overnight. Move the extract
to sample a bottle. The yield percentage of each concentrated were calculated.

(Soaking)

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( Filtration)

Extraction using rotavapor

5.4 Result

Mass of concentrated
Herbs
extract (g),x

Percentage of yield,
=

x
100 g

Curcuma aeruginosa Roxb.

Centella asiatica (L.) Urban
Tinospora crispa (L.) Hook F. & T
Merremia mammosa Chois.
Curcuma xanthorrhiza Roxb.
Boesenbergia pandurata (Roxb.)

10.44
4.07
2.54
10.15
3.02
8.95

10.44
4.07
2.54
10.15
3.02
8.95

Schlecht
Syzygium polyanthum Wigh Walp

6.50

6.50

CHAPTER 6.0: Suggestions

Regarding any inconveniences relating to the internship, there was only one minor
inconvenience that I can recall. Since I needed to gain access to the Microbial lab, it was
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necessary for me to have a key to unlock the door. I generally needed to find postgraduate
students or staffs that had a key which required me to interrupt them to have the room
unlocked. While they were always helpful in doing this, it would have been less disruptive
and efficient if I could have had a key to the lab. I also suggest that the duration for this
internship should be longer in order to give students enough time to learn and trained more in
chemistry industry.

CHAPTER 7.0: Conclusion

Overall, I enjoyed the internship. I feel that this internship here in IBS gives students
a great opportunity to learn more about the equipment used in the lab such as a rotavapor . It
provided me a deeper knowledge of lab equipment and practices. Besides, it is an advantage
when using this equipment in the workforce. This internship gave me the opportunities to
coordinate the lab requirements and learn how to meet the expectations of diverse personnel,
which will prepare me for a real world work experience. It also taught me to first understand
how equipment functions and then take this understanding and figure out the solution for any
problem that might occur.

REFERENCE

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Ekta Menghani, Sita kumara sharma (2012). Antimicrobial Efficacy of Piper cubeba and
Tribulus terrestis, World Journal of Pharmacy and Pharmaceutical Sciences, Volume 1,
Issue 1, 273-279.
Elin Yulinah Sukandar, Nethiyakalyani Sunderam and Irda Fidrianny (2013). Activity of
Kaemferia Pandurata (Roxb.) Rhizome Ethanol Againts MRSA, MRCNS, MSSA,
Bacillus subtilis and Salmonella typhi, Pakistan Journal of Biological Sciences.
Kyung-Min Park, Jeong Han Choo, Jong-Hee Sohn, Sun-Hee Lee and Jae-Kwan Hwang
(2005). Antibacterial Activity of Panduratin A Isolated from Kaempfreria Pandurata
againts Phorphyromonas gingivalis, Food Sci. Biotechnol., Vol. 14, No. 2, pp 286-289.
Mulyadi Tanjung, Tjitjik Srie Tjahjandarie, Mulya Hadi Sentosa (2013). Antioxidant and
Cytotoxic Agent from The Rhizomes of Kaempfreria pandurata, Asian Pasific Journal
of Tropical Disease, 3 (5): 401-404.
Yanti, Yaya Rukayadi, Kwan-Hyoung Lee and Jae-Kwan Hwang (2009). Activity of
Panduratin A Isolated from Kaempfreria pandurata Roxb. Against Multi-species Oral
Biofilms In Vitro,Vol. 51, No. 1, 87-95.

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