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in order to promote transparency and accountability in the working of every public authority,
and whereas the attached publication of the Bureau of Indian Standards is of particular interest
to the public, particularly disadvantaged communities and those engaged in the pursuit of
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timely dissemination of this information in an accurate manner to the public.
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Jawaharlal Nehru

IS 5055 (1996): Lecithin, Food Grade [FAD 8: Food

Additives]

! $ ' +-
Satyanarayan Gangaram Pitroda

Invent a New India Using Knowledge

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Knowledge is such a treasure which cannot be stolen

IS 5055 : 1996

Indian Standard
LECITHIN, FOOD GRADE - SPECIFICATION
( First Revision)

ICS 6722020

e BIS 1996
BUREAU OF INDIAN STANDARDS
MANAK BHA VAN, 9 BAHADUR SHAH ZAF AR MARG
NEW DELHI 110002

September 1996

Price Group 4

AMENDMENT NO. I FEBRUARY 2006

TO
IS 5055 : 1996

LECITHIN, FOOD GRADE-SPECIFICAnON

( First Revision)

I Page I. Table I, S'/ No

(viu), coI3]- Substitute '10' [or '100'

(FADlI)

Reprography Unit, BIS, New Deihl, Indra

Food Additives Sectional Committee, FAD 8

FOREWORD
This Indian Standard ( First Revrsion ) was adopted by the Bureau of Indian Standards, after the draft finahzed
by the Food Additives Sectional Committee had been approved by the Food and Agnculture DIVISIon Council

With the Increased production of processed foods, manufacturers have started adding a large number of substances, generally In small quantities, to Improve the appearance, flavour, texture or storage properties of the
processed foods As certain impunues In these substances could be harmful, It IS necessary to have a strict
quahty control of these food additrves A senes of standards was, therefore, prepared by this Institution to cover
punty and rdennfication of these substances These standards would help In checking purity, which requires to
be checked at the stage of manufacture, for It IS extremely difficult (and In many cases impossible) to detect the
Igtpunty once these substances have been added to the processed foods Besides, these standards are Intended to
guide the indigenous manufacturers In making their product conform to specificanons that are accepted by
SCientists, health authonnes and international bodies, and the consumer mdustnes to use them Within the quantity permitted by the health authonties
Lecithin, WIdely used as ann-oxidant and emulsifler, IS permitted under the Prevention of Food Adulteration
Rules, 1955, as well as the Fruu Product Order, 1955
This standard was first ISSUed In 1969 It IS being revised to make the followmg changes/additions
a) To upgrade the standard by increasing the punty limit
b) To provide a separate clause for descnpuon including the solubrhty property to keep It mime with
food chemical codex NRC
c) To Incorporate the amendment No I Issued to earher version
d) To include the requirements for heavy metals and peroxide value, and their methods of test
e) To provide mformation as to whether It IS of arurnal ongm or vegetable ongm or both and the
expiry date under marking clause
Cbemical Names and Formulae
The recogmzed chemical names are lecnhm. phospholutem, phosphandes, and phosphohpids Food grade leerthm IS a complex mixture of acetone Insoluble phosphatides consistmg chiefly of phosphaudly choline, phosphaudyl ethanolamme, phosphatidic acid, and phosphatidyl mosrtol combined With various amounts of other
substances, such as tnglvcendes, fatty acid and carbohydrates Formulae for vanous phosphatrdes are given
below

CH20COR

CH20COR

I
CHOCOR

CHOCOR

1\

I o~

CH2~C~CH2 N ICH]I]

CH20POCH2CH2 NH2

OH

PHOSPHAl'IDYl CHOLINE
(LECITHIN FRACTION)

PHOSPHATIDYl ETHANOLAMINE
(CEPHALIN FRACTION)
CH20COR

I
CHOCOR
1

o~

CH2- j P - O
OH

PHOSPHATIDIC

ACID

0
HO

OH

HO

OH

PHOSPHATIDYL INOSITOL

= vanous saturated and unsaturated fatty acids groups

( Continued on third cover page )

( Connnued from second cover page)

In the preparatIon of dus standard considerable amount of assistance has been denved from the Food Chemical
Codex, Thud Bdmon, National Academy of SCiences, National Research Council, Washmgton DC, USA
For the purpose of deciding whether a particular requirement of this standard IS complied with the final value
observed or calculated, expressmg the result of a test or analysis, shall be rounded off In accordance with IS 2 1960
'Rules for rounding off numencal values ( revised)' The number of sigmflcant places retained In the rounded off
value should be the same as that of the specified value III this standard

IS 5055 : 1996

Indian Standard
LECITHIN, FOOD GRADE -- SPECIFICATION
( First Revision)
1 SCOPE
This standard prescnbes the requirements and the
methods of samphng and tests for lecnhm, food grade
2 REFERENCFS

IS No

sodium III a soft glass tube, and heat to redness Plunge

while hot into about 10 mI of disnlled water, heat to
boihng and filter Add a few crystals of ferrous sulphate
to the filtrate, bod and add dilute sulphunc acid until
Just aCIdIC Allow to stand for 15 mmutes, filter and
wash A blue precipitate shall be formed

71tle

4.1.3 Reflux I g of'lecitlun for I hour WIth 25 011 of 0 5 N

1070 1992 Reagent grade water t tlurd revrston v
1699 1995 Methods of sampltng and test for
syntheuc food colours ( vecond re~r"on)
5106 1996 Sodiurn carboxymethyl cellulose, food
grade ( second revtsum s
3 DESCRIPTION

3, I The matenal is a VISCOUSsemr-hquid With a charactcrtstic odour It IS light yellow to brown depending
upon whether It ISbleached or unbleached Lectthm IS
obtained from egg or edible vegetable oilseeds by
SUItable dehydration or solvent extraction usmg food
grade solvents It may also be obtained from arumal
sources Fdible drlucnts, such as cocoa buller and
vegetable OIls may be added to Improve functional and
flavour charactensucs
3,2 The matenal is insoluble In water but charctensncally
hydrated WIth swellmg It IS Insoluble III acetone but
50luble In chloroform and benzene The 'Iccithm fraction' IS soluble while ccplialin fraction IS insoluble in
ethanol
NO I J[he solubslrty I'" mtended only av mformauon
regarding approximate ..olubihty and 1\ not to be
considered a" a quality requuement and IS 01 mmor srgruficance 8!1. a means of tdenuf....atron (If detcmunanon of
puntv and dependence must he placed on other ..pecrticalion"

4 REQUIREMENTS

ethanohc potassium hydroxide When cooled to O'C

precrprtatc of potassium soap shall be obtained

.i

4.2 Gossypol Te~t

The total gossypol content III cottonseed lccitlun shall
1I0texceed 'i percent by mass The method for detemunauon of gossypol IS given III Annex A
4.3 The material shall also conform to the reqiurements
giveu m Table I
Table 1 Requirements for Lecithin, Food Grade
51

Characterasbc

No

Requirement

Methud of Test, Ref to

Annex uJ

thl<
Standard
(2)

(t)

r)

Purity, as acetoneInsoluble
residue,
percent by mass, Min

II)

Morsture, percent hv
rna...... Max

III)

Benzene
insoluble
matter, percent by
mass. Max

tv)

Acrd value, Max

v)

Ar~n1c

(3)

(4)

62"

13

Clauve ut

IS 1699
1995
(5)

c
03

35
15

( "-_ As ),

mglkg.Max
VI)

Lead (a, Pb), mg/kg,

15

10

Max

4,1 Identifieatiod

VII)

4.1.1 Yellow Precipitate WIth AmmOnium Molybdate

VIII)

Igmte I g ofthe matenal With 2 g of anhydrous sodium

carbonate Cool and dissolve the residue In 5 ml of
water and 5 ml of nunc acid Add 5 ml of ammoruurn
molybdate and heat to boiling A yellow prccpnate
shall be formed

4.1.2 Blue Prectpttate with Ferrous Sulphate

Fuse about 0 5 g of the material WIth about 0 05 g of

Heavy metals .., (Pb)

mglkg,Max

40

Peroxide value Max

100

(i

II

"Eqervalents to 2 2 percent of phovphornus when deter

mined, by the method given In Annex J)

5 PACKING AND MARKING

5.1 Packing
The matenal shall be securely packed in well-filled
containers WIth nummum access to hght and arr The

IS 5055: 1996
5.2. tHIS ( erufic alum Marking

containers shall be such as to preclude contamination

of the contents WIth metals or other rmpunnes
5.2

The product may also be marked with the Standard

Malk

Markin~

5.2.1.1 The use of the Standard Mark IS governed by

the provrsions of the Bureau ofIndian Standard, Act,
1986 and the Rules and Regulations made thereunder
The details of condiuons under whtch the licence for
the usc of the Standard Mark may be granted 10
manufacturers or producers may be obtained from Ihe
Bureau of Indian Standards

Each container shall be legibly and indelibly marked

WIththe following mfonnauon
a) Name ofthe matenal mcludingthe words 'Food
Grade'
b) Name and address ofthe manufacturer,
c) Mrmmum net content,

6 SAMPLING

d) Batch or code number,

Representative samples of the matenal shall be drawn

accordrug to the mel hod prcscnbed 111 4 of IS 1699
I')')'i

c) Whether made from vcgctable ongm or animal

ongm or both,
l)

7 QUALrrv OF REAGENTS

ExpIrydate, and

Unlcss specified otherwise, pure chemicals and distilled

water (lee IS 1070 1992) shall be cmployed In tests

g) Any other requircrncnts as specified under the

Standards of WelKhl' and
Meavures
i Packuged Commodrue-q Ruin, 1977/l'revenII'm oj Food Adulterauon Ruin, 1955

NO II
Pure chemu.al-, vhall mean chermcals that du
not cuntatn tmpuntrc-, which Kneel the experimental
TL"UIt...

ANNEX A
( ( 'Iausc 4 2 )
DETERMINATION OF TOTAL GOSSYPOL
A-I PRINCIPLE

keeping the water at a gentle bot! The water-bath should

be equipped WIth clamps 10 securely hold the volumetnc flasks IlIIlIIer'iC<! 111water

The term 'total gossypol' designates 'free gossvpol

'bound gOSSYPOl' and closely related pigments which
after hvdrclvsi and reaction wrth an orgamc anunc
(p-ll!llSldme or amhnc ) give a product idenncal speclrophotomctncally WIth that obtamed from pure gossypol and the sallie reagenr In this method, total gossvpol is completely removed from lccuhm m a 10-mmute
cxtracuon dunng which gossypol IScomplexed With ncutrnhzcd I-olll1lno-l-propanol m dunethvl fonnanudc The
difference III absorption of aliquot portions of the
extract before and after reaction With amlmc SCIVCS as a
measure of the total gossvpol content. and allows proper
correction for the background absorption of the extracts

A-3 REAGENTS
A-3.1 j"oPropyl Alcohol- Hexane Mixture
MIX(,0 volumes 01 reagent grade I\opropyl alcohol and
40 volumes of commercial hexane
A-J.2

A-2 APPARATlJS

NOTE ~ ,-ammu-i-prupanol may be redrstrlled if coloured

Its boiling stam. I" 1RR"C, and It may be conveniently
distilled under water pump vacuum

A-2.1 Photoelectric Colorimeter - With a filter

havmg a maxunum transmutance m the VICllllty of 440
nm or ,I spectrophotometer rsolanng a band at 440 nm
A-2.2

Pillette~,

Volumetric

A-3.3 Anilinc

1,2,4,6, I! and III 1111

A-2.J Flasks, Volumetric - 25.50, and

J()()

Reagent grade, redrsnlled over LInC dust Store III a

refrigerator and redrstil when the absorbance of the
reagent blank exceeds 0 022

ml

A-2.4 Insulated Wah:r Bath

I'hcnnostancallv controlled 10

le.

Cllmplexin~Reagent

Pipette 2 Ill] of1-arruno-l-propanol and 10 ml of glacial

aceuc acid mto a 100-ntl volumetric flask, cool to room
temperature, and dilute to volume with dimethyl
fonnanudc ( N-N dimethyl formarrude, redistilled
between] 'i2to 151C) TIllS reagent IS stable for one
week after preparation

and eapable of

A4 STANDARD GOSSYPOL SOLUTIONS

2

IS 5055 : 19%
A-4 STANDARD GOSSYPOL SOLUTIONS

shall probably vary With each concentration of

gossypol m which case a cahbranon curve should be
plotted and used

A-4.t Weigh accurately 25 rng of pure gossypol or

27 9 mg of pure gossypol acetate, dissolve m and make
up to 50 ml volume WIth the complexmg reagent If
exactly 25 mg of pure gossypol IS used, the solution
shall contarn 0 5 mg/ml

A-5 SAMPLE PREPARATION

Heat the sample to approximately 50"C, nux well and
filter Store at O'C, If analysis ISnot done rmmcdratelv
The analytical sample should contam from I to 5 mg of
gossypol For maximum precision. the aliquot used
should contain 0 I mg of gossypol

A-4.2 Calibration Curve

Pipette 2, 4,6, 8 and 10 mI of standard gossypol solution
into 50-m1 volumetric flasks

A.() PROCEDURE

A-4.J To each standard add sufficient complexmg

reagent 10make up the total volume to 101111 Use 10 ml
of the complexmg reagent as a blank

A-6.t Weigh sufficient quanutv of the sample matenaJ

as directed 111 A-5.1 into a 50-1ll1 volumetnc flask Add
IIIml of the complexmg reagent

A-4.4 Heat the flask contanung the standards and the

blank m a boihng water bath (100" C) for 30 nunutcs,
cool and dilute to volume With the I vopropyl alcohol-

A-6.2 Use to ml of the cornplexmg reagent as the

reagent blank

hexanenuxture

A-6.J Heat both the sample and the blank III a borlmg
water-bath for 30 minutes, cool to room temperature,
dilute to volume with Isopropyl alcohol hexane mixture
and mix Filter through Whatman No, I or equrvalem
filter paper and collect the filtrate 111 a small glass stoppered flask

A-4.5 PIpette in duplicate 2 1111 ahquots of each diluted

standard and of the blank mto 25-ml voluiuetnc flaks
DIlute one set of ahquots to volume With the Isopropyl
alcohol-hexane mixture and reserve as reference
\DIU/IOn,

A-6.4 PIpette duphcate ahquots (see A-5.1) of the

filtered extract and of the reagent blank into 25-mJ
volumetric flasks

A-4.6 To the other set of ahquots, add 2 ml of atuhne.

heat in aborhng water-bath for 30 mmutes, cool to room
temperature and dilute to volume With Isopropyl
alcohol-hexane mixture Allow the llask to stand at room
temperature for one hour after drlunon and nuvmg

A-6.5 Dilute one of the ahquots to volume With the

Isopropyl alcohol hexane mixture and reserve as reference solutions

A-4.7 With a spectrophotometer, deternunc the

absorbance of the reagent blank at 440 nm usmg the
dilute blank aliquot Without am line as a reference solution

A-6.6 To the other aliquot, add 2 ml of'amlmc, develop

the colour and determine the corrected absorbance oil
440 lUll a" outlined in A-4. 7 and A-4.8.

A-4.S Deterrmne the absorbance of each gossypol

standard reacted with amline, usmg the appropriate
diluted stand..rd as a reference soluuon Subtract the
absorbance of the reagent blank from that of each
standard to obtam the corrected absorbance

A-7 CALCULATION
Dctcrnune the gossypol (111 milhgrams) In the sample
aliquot by means of the calibration curve on the
calibration factor

A-4.9 Calculate the cahbratron factor by dividmg the

number of'rmlhgrams of gossypol in the 2 OIlaliquot of
each standard by the appropnate corrected absorbance
Average the factors for all the gossvpol standards
When a photo-elccmc colonmeter IS used, the factors

Total gossypol,
percent by
mass

mg gossypolm sample aliquot

Mass of sample in g

Volume of
ahquot used
for analysis

IS 5055 : 1996

ANNEXB
[ Table 1, Sl No (I)]
DETERMINATION OF PURl1Y
nuxmre rn a water bath until the lecithm melts, bul avotd
evaporation of the acetone SUr unul the sample IS
completely dismtegrated and dispersed, and then
transfer the tube mto an Ice bath, dull for S rmnutes,
remove from the Ice bath, and add about one half of the
required volume of phosphatide acetone solution, previously chilled for S minutes In an ICe bath Stir the
mixture to complete dispersion of the sample, dilute to
40 ml with chilled phosphatide acetone solution (S"C),
agam stir and return the tube and contents to the Ice
bath for 15 minutes Attheendofthe 15 mmutes chill109 penod sur agam wlule stili m the Ice bath, remove
the snrnng rod, temporarily supporting It III a vertical
UPSide down posmon,and centrifuge the mixtureImmediately at about 2 000 rev/nun for 5 minutes Decant the
supernatant liqUid from the centrifuge tube, crush the
centnfuged solids With the same snrrmg rod previously
used, and refill the tube to the 40-ml mark With chilled
(S"C) phosphatide acetone solunon, and repeat the chillmg, snrnng, centnfugation, and decantation procedure
previously followed After the second cenmfugauon
and decantation ofthe supernatant acetone, agam crush
the solids With the assigned surnng rod, and place the
tube and ItS contents m a honzontal positron at room
temperature unul the excess acetone has evaporated
MIX the residue agam dry the ceutnfuge tube and ItS
contents at lOi"C for 4~ nun III a forced draft oven, cool
and weigh

B-t REAGENTS
B-I.l Purification of Phosphatide!
Dissolve ~ g of phosphaudes from previous acetone
insoluble maucr deternunanon in 10 ml of petroleum
ether and add 25 ml of acetone 10 the soluuon Transfer
approxnnatcly equal portions ofthc precipitate to each
of two 40-ml centnfuge tubes using addtUonai portions
01 acetone to facrlrtate the transfer SUr thoroughly,
dllulcl040 ml wnh acetone sur agam.cfull for I ~ nurun
au tee-bath stir agam, and then ccntnfuge for 5 nun
Decant the acetone crush the solids With a stimng rod,
rcfill the tube WIth acetone, stir, chill, centrifuge. and
decant ,IS before The solids after the second centnfugallon require no further punficauon and may be used
for prepanng the phosphatide acetone solution Five
grams of the punfied phosphaudes are required to
saturate about 16 lures of acetone
B-I.2 Phosphande Acetone Solution
Add a quanntv of punficd phosphaudes to sufficient
acetone previously cooled to a temperature of about

'Ie

to form a saturated solution. and mamtam the

mixture at this temperature for 2 hours, shakmg rt
\ igorously at IS mmutcs intervals Decant the soluuon
through ,I rapid filter paper avoidmg the transfer of any
undissolved solids to the paper and conducting the
filtr.uron undcr rctngcr.ued condiuons (nol above ~'C)

B-3 CALCULATION

8-2 PROCEDURE

Acetone msoluble residue

percent by mass

,",oUln,1 portion ofthe matcnal bv warnung II III a water

b.nh at ,I temperature not cvcccdmg 60 C and then
nuxing 1\ thoroughlv Transfer 2 g of a well-mixedsample
accurately weighed IIIto a 40-m! centrifuge tube
previously tared With a glass snrnng rod Add IS 011 of
phosphatide acetone soluuon from a burette Warm the

(1 00 R!,~)

where

R = mass ofthe residue,

S= mass of the sample, and
B = benzene msoluble matter (see E-2.1 )

ANNEXC
[ 7able 1, Sl No. (II) ]
DETERMINATION OF MOISnJRE

c-i

sample as evenly as practicable to a depth of about

5 rnm Place the bottle contammg thc sample
(uncovered) in the oven mamtamed at lOS" :t I"C
Remove the bottle from the oven after one hour, close
the bottle promptly and allow It to come to room
temperature 10 a desiccator Weigh It

APPARATUS

C-1.1 Oven -- mamtamed alI05":t I"C

C-1.2 Weighing Bottle -

glass stoppered, shallow

C-2 PROCEDURE
C-2.1 Weigh accurately about 10 g of the well mixed
sample m the tared werghmg bottle Drstnbute the

C-2.2 Calculate loss on drying, percent by mass

IS 5055 . 1996

ANNEXD
(Table I)
DETERMINATION OF PHOSPHORUS
1>-1 REAGEN'IS

1>-2 PROCEDURE

D-I.1 AmmoniaSolution -25 percent (wlv)

MIx about 0 5 g, accurately-weighed sample WIth ~ g of

a mixture of equal parts of anhydrous sodium carbonate
and potassium rutnte Place the matenal in d crucible
LOverIt with a layer of anhv drous sodium carbonate
and igrutc Dissolve the residue III dilute rutne acid and
make It alkaltne WIthammoma solution a nd add a slight
excess of solution of magnesium ammo sulphate dud
filter Wash thc precrpttatc with arnmoma solution (2 '5
percent lillY), igrute and weigh the residue of magncsium pyrophosphate (Mg,P,o,) One gram ofthe iesrdue IS equivalent to 0 278 6 g of phosphorus

1>-1.2 Potas~ium Nitrite

0-1.3 Sodium Carbonate - anhydrous

I>- 1.4 Magnesium Amino Sulphate

DIssolve 10 g ofmagnesmm sulphate and 20 g of ammonium chloride In !\O ml of water and 42 mI of dilute
ammoma solution set aside for a few days In a wellclosed bottle decant and filter

0-1.5 Nitnc Acid - Dtlute

ANNEXE
[ Table 1, Sl No (Ill)]
DETERMINATION OF BENZENE INSOUlBLE MATTER

Eo. PROCEDURE

1O,"C for an hour cooled m a desiccator and weighed

Wash the flask with two successive 2,-ml portions of
benzene and pass the washings through the filter
Dry the funnel at 105'C for an hour cool to room
temperature III a desiccator. and weigh

Soften a poruon of the matenal by warrmng It at a temperature not exceeding 60'C and then nux it thoroughlv
Weigh 109 of a previously well nu xed sample mto a
1 ,O-ml wide-mouth Erlenmeyer flask add Ino m1 ofbenzene and shake until the lecrthm IS dtssolved Frlter the
solunon through a 30 ml Conung C' porosityor equivalent filtenng funnel whichpreviously has been dned at

E-2 CALCULATION

From the gam m mass of the funnel calculate the

percent of the benzene insoluble matter in the sample

ANNEXF

[ Table 1. Sl No (IV)]
DETERMINATION OF ACID VALUE

F-l PROCEDURE

hydroxide to a pink end point which persists for

5 seconds

Soften a portion ofthe matenal by wamung It in a water

bath at a temperature not exceedtng 60'C and then nux II
thorougWy Transfer about 2 g of the well-rruxed sample
mto a 250-00 WIde-mouth Erlenmeyer flask, and
dissolve It m 50 ml of petroleum ether To tlus solution,
add 50 ml of alcohol, previously neutrahzed to phenolphthalein WIth 0 I N sodium hydroxide, and mix well
Add phenolphthalein and titrate WIth 0 I N sodium

F-2 CALCUlATION
Calculate the number of rmlhgrarns of potassium
hydroxide required to neutrahze the acids III one gram
of the sample by mulnplymg the number of rrulhhtrcs of
o I N sodium hydroxide consumed III the utrauon b) , 0
and dividmg the result by the weight of the sample

IS 5055: '996

ANNEXG
[ 7ahle 1, Sf No (VII) 1
DETERMINATION OF HEAVY METAlli
G-' Proceed as given In Annex F of IS
except that F-2 3 shall be as follows

~106

1996

To each tube add 10 ml of freshly prepared

hydrogen sulphide, mix and allow to stand for 5

nun and view over a white surface The colour of
Solunon B shall not be darker than that of
Solution A

ANNEXH
[ 7able 1, st No (VIII) 1
DETERMINATION OF THE PEROXIDE VALUE

H-4I PRINOPLE
OXidation of potassium Iodide by the peroxides of
lecuhm and utranon of the iodine hberated usmg
standard sodium thiosulphate solution
H-I APPARATUS
H-1.1 Analytical Balance
H-I.2 Apparatus - As shown in Fig

boilmg for three to four nunutes Stop heatmg and

immcdrately disconnect the condenser Quickly add 50
rnl of water through the glass tube Remove the glass
tube and cool the flask to room temperature under the
water tap Titrate With sodium throsulphate until the
aqueous layer becomes pale yellow Add I ml of starch
solution and continue the titration until the blue colour
IS discharged Shake the flask well dunng the titration
to ensure the complete extraction of iodme from the
non-aqueous layer

H-2 REAGENTS

H-2.2 Chlorofonn

H-3.4 Obtam a blank turanon value bv repeanng the

complete procedure (H-3.1 to H-3.3) but WIthout addmg the sample

H-2.3 Potassnnn Iodide

H-4 CALCULATION

H-2.4 Sodium Thiosulphate (0 I molll or 00 Imol/l )

The Perovidc value IU the sample, III rrulllrequrvalents

per kilogram IS given by

H-2.1 ALctic Acid GI.lcidl

0-2.5 Starch Solution (approximately I percent m/v)

H-3 PROCEDURE
H-3. t Place 10 ml of glacial acetic acid and 10 ml of
chloroform In the IOO-ml flask Fit the glass tube and
reflux condenser and gently boll the mixture for two
minutes to expel all dissolved au Dissolve I g of potassrum iodide in I 3 ml of water and add this solution to
the nuxture in the flask taking care that the boihng IS not
interrupted

H-3.2 If a yellow colour appears at this stage the

determmatmn must be rejected and repeated using fresh
reagents

IOOOxaXU,-V,)

I',

volume rn rrulhhtrcs oftluosulphate solution

required for the titration of the sample

V, = volume III rrnlhhtres ofthiosulphate solution

required for the titration at blank.

a = concentration

of sodium
solution m molll, and

/n,

H-3.3 Accurately weigh, to the nearest mg, about I g of

the sample and, after a further two mmutes of boihng,
add the weighed sample to the contents of the flask
again taking care that the boihng remains continuous
For this purpose the sample should be contained m a
nucrobeaker wluch may be lowered through the glass
tube With a glass rod. the bottom ot wluch has been
SUItably shaped as shown ID the diagram The
condenser may be removed for a short I.tme Continue

/no

where

tluosulphate

= uuual mass m grams ofthe sample taken

NOIL~

J The choice of the concentranon of the sodium

throsulphate used depends on the anucipated IJITalJon value
II less Ihan 0 3 ml of 0 1 molll sodium thiosulphate
" required, repeal the deterrnmalJon usmg 0 0 I mol/l
sodium thlo.ulphale

2 The determmanon should

.1rong hght

not

be earned oul

In

IS SUSS: 1996

REFLUX CONDENSER

GLASS

MICRO BEAKER
- EXT OIA 20mm
-HEIGHT.
35m SOmm

TUBE

- LENGTH 250 m m

-10 22mm

ROUND RonOM
FLASI< 1100m\)

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Bureau of Indlu Studard.

BIS ISa statutory msUtutlon established under the Bureau oflndion Standards Act. 1986 to promote harmonIous
development of the acUVlUes of standardIzation, markmg and qualIty certification of gooda and attendIng to
connected matters in the country

Copyrlpt
BIS has the copynght oeall ItSpubllcauons No part of these publications may be reproduced In any form WIthOUt
the pnor permrssicn 10 wnung of BIS Tlus docs not preclude the free use, 10 the course of rmplementmg the
standard, of necessary detaJls, such as symbols and SIzes, type or grade desrgnauons Enqumes relatmg to
copynght be addressed to the Director (Pubhcanons), SIS
RevIew of Indian Standard.
Amendments are Issued to standards as the need anses on the baSISof comments Standards are also reviewed
penodtcally, a standard along with amendments 15 reaffirmed when such review indrcates that no changes arc
needed, If the review 10dlcates that changes arc needed, It IS taken up for revisron Users of Indian Standards
should ascertalA that they are m possessron of the latest amendments or edmon by referrmg to the latest Issue
of 'SIS Handbook' and 'Standards Monthly Addmons'
ThIS Indian Standard has been developed from Doc

No FAD 8 (467)

Amendment. baued Since Publication

Amend No

Date of Issue

BUREAU

OF

INDIAN

Text Affected

STANDARDS

Headquarters
Manak Bhavan, 9 Bahadur Shah Zafar Marg, New Deihl 110002
Telephones 323 0 I 31, 323 94 02, 323 83 75

Telegrams Manaksanstha
(Common to
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Regional Offices

Telephone

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{ 3233841

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NEW DELHI 110002

Eastern

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CALCUTIA 700054
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3378499, 3378561
{ 3378626, 337 8662
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{ 602025

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23502 16,2350442
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Western Manakalaya, E9 MIDC, Marol, Andhen (East)

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PnnlOd II NewIndsoPrant. .

""'II,

Khw)1, Inc'"