Efek Minyak Atsiri Daun Nilam (Pogostemon cablin Benth) terhadap Kadar IL-6 Serum dari Tikus

Wistar yang Diinduksi Metanil Yellow

Yuliana Ayu Rahayu Manik, Helmin Elyani, Doti Wahyuningsih
Fakultas Kedokteran Universitas Islam Malang
email: ayuanamanik@yahoo.com
ABSTRAK
Pendahuluan Penyalahgunaan Metanil Yellow pada makanan, menyebabkan nitroanilin masuk tubuh dan
meningkatkan radikal bebas. Hal ini akan mengaktivasi sekresi Interleukin 6 (IL-6) serum akibat adanya
inflamasi. Minyak atsiri daun nilam (Pogostemon cablin Benth) memiliki efek antioksidan. Penelitian ini
dilakukan untuk membuktikan efek minyak atsiri daun nilam terhadap IL-6 pada tikus wistar yang diinduksi
Metanil Yellow.
Metode Penelitian eksperimental laboratorium post test group only design pada tikus strain wistar jantan yang
dibagi menjadi lima kelompok; kontrol negatif, kontrol positif (Metanil Yellow 80 mg/kgBB/hari) dan kelompok
diinduksi personde minyak atsiri daun nilam selama 5 hari dengan dosis konsentrasi 3%, 6%, dan 12%. Kadar
IL-6 diukur secara ELISA (Enzyme Linked Immunsorbent Assay) indirect. Analisis data menggunakan uji one
way ANOVA dan dilanjutkan post hoc test Least Significant Difference (LSD), bermakna bila p<0.05.
Hasil Induksi Metanil Yellow meningkatkan 41% kadar IL-6 serum (p<0,05). Pemberian minyak atsiri daun
nilam dosis konsentrasi 3% mampu menurunkan 15,58% dan dosis konsentrasi 6% mampu menurunkan
39,02% kadar IL-6 serum dibanding kontrol positif (KP) (p<0,05). Pemberian dosis konsentrasi 12%
mengakibatkan kematian pada hewan coba.
Kesimpulan Pemberian minyak atsiri daun nilam (Pogostemon cablin Benth) konsentrasi 3% dan 6% mampu
menurunkan kadar IL-6 serum tikus wistar yang diinduksi Metanil Yellow. Pada konsentrasi 12% merupakan
dosis toksik.
Kata Kunci Minyak atsiri daun nilam, kadar IL-6 serum, Metanil Yellow.

PATCHOULI LEAVES ESSENTIAL OIL EFFECT ON INTERLEUKIN 6 SERUM LEVEL IN

METANIL YELLOW- INDUCED WISTAR RAT
Yuliana Ayu Rahayu Manik, Erna Sulistyowati, Doti Wahyuningsih
Faculty of Medicine Malang Islamic University
E-mail: ayuanamanik@yahoo.co.id
ABSTRACT

Introduction Metanil Yellow (MY) ion foods that were misused, make nitroanilin came into
the body and was to increase free radical. This mechanism will increase the number of IL-6
serum due to inflamation. Essential oil of patchouli (Pogostemon cablin Benth) leaf has an
active antioxidant substances that can prevent the formation of ROS. The researchers' goal
was to determine the effect of patchouli leaf essential oils on the number of IL-6 serum in
acutely induced Metanil Yellow by male wistar rats.
Methods Using experimental laboratory research of post test group only design, the Wistar
rats were divided into five groups: negative control, positive control (Metanil yellow 80
mg/kgWeight/day) and treatment groups of Patchouli essential oil which are induced by 3%,
6%, and 12% dosages respectivety for 5 days via sonde. IL-6 level were measured with
indirect ELISA (Enzyme Linked Immunsorbent Assay). The data were analysed using oneway ANOVA assessment, followed by post hoc test of the Least Significant Difference (LSD).
The result is significant when p0.05.
Results Induction of Metanil Yellow can significantly increase the number of serum IL-6
serum up to 41%. The supplementation of patchouli essential oil concentration of 3% could
reduce 15,5% and concentration of 6% could reduce 39% the number of IL-6 compared to
the positive (KP). The supplementation concentration 12% resulting in death.
Conclusion The supplementation of patchouli leaf essential oil (Pogostemon cablin Benth)
concentration of 3% and 6% could reduce the number of IL-6 serum in acutely induced
Metanil Yellow by wistar rats. Concentration 12% is dosage toxic.
Keywords patchouli leaf essential oil, IL-6 Serum Level, Metanil Yellow