Professional Documents
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Microbiology
By Arcibel B. Bautista
Introduction
Microbiology the study of very small living
organisms
Kinds of Microbes
Bacteria
Viruses
Microbes
Fungi
Protozoa
Algae
Branches of Microbiology
study of microbiology broadened and became
more specialized
Clavaria: a fungi
bacteriology
study of bacteria
mycology
study of fungi
parasitology
study of protozoa and parasitic worms
immunology
study of immunity
virology
study of viruses
Areas of Microbiology
Bacteriology
Phycology
Virology
Basic
Mycology
Parasitology
Protozoology
Areas of Microbiology
Agricultural
Veterinary
Sanitary
Industrial
Applied
Clinical
Environmental
Medical
Food
Nature of Microorganisms
4.5 billion years ago, Earth was formed.
Nature of Microorganisms
PIONEER IN MICROBIOLOGY
PIONEER IN MICROBIOLOGY
Robert Hooke: 1635 1703
discovered & described aslittle
boxes on a thin slice of cork
came to be known as cells, the
worlds smallest structural unit
beginning of the cell theory:
all living things are
composed of cells
Cell Theory
All living organisms are composed
of cells. (They may be unicellular or
multicellular.)
The cell is the basic unit of life.
Cells arise from pre-existing cells.
PIONEER IN MICROBIOLOGY
First microscope
PIONEER IN MICROBIOLOGY
3. Louis Pasteur
discovered alcohol
fermentation and
Pasteurization
Louis Pasteur
Spontaneous Generation
proved that spontaneous is a result
of the presence of microorganisms in
the air or the fluids themselves
PIONEER IN MICROBIOLOGY
4. Robert Koch
Germ Theory of Disease
Kochs Postulate
Kochs bacilli
Robert Koch
Kochs Postulate
1. The bacteria must be present in every
case of the disease.
2. The bacteria must be isolated from the
host with the disease and grown in pure
culture.
3. The specific disease must be
reproduced when a pure culture of the
bacteria is inoculated into a healthy
susceptible host.
4. The bacteria must be recoverable from
the experimentally infected host
(http://www.medterms.com/script/mai
n/art.asp?articlekey=7105)
Kochs Postulate
Aseptic Technique
basis of aseptic
technique
techniques used to
prevent
contamination by
unwanted
microorganisms
standard
laboratory practice
fermentation and
pasteurization
germ theory of
disease
Vaccination
Edward Jenner developed the first vaccine in 1798 for
smallpox
In 1880 Pasteur discovered the use of a virulent
bacteria for a vaccine against fowl cholera and
coined the term vaccine
Edward Jenner
Chemotherapeutic Agents
synthetic drugs
chemically prepared in the lab
antibiotics
substances produced naturally by
bacteria and fungus
both synthetic drugs and antibiotics
inhibit the growth or kill other
microorganisms
Paul Ehrlich
first use of a
chemotherapeutic
agent in 1910
used an arsenic
containing compound
to treat syphilis
repeated his
experiments with minor
changes 605 times
before he found a
concentration that
worked
Paul Ehrlich
Penicillin
Alexander Fleming accidentally discovered that
mold (Penicillium) inhibited the growth of bacterial
cultures in his lab in 1928
usefulness of penicillin was not apparent until the
1940s
clinically tested and as a result of the test, it was
mass produced
World War II spurred on the production of
penicillin as it was initially used for the war effort
Modern Microbiology
new and different directions that the
study of microbiology is going
towards
drug resistance
Drug Resistance
result of genetic changes
in microbes
production of microbial
enzymes that inactivate
antibiotics
surface changes in
microbes
disallowing antibiotics
from attaching to it
preventing antibiotics
from entering the
microbe
New Vaccines
as new diseases emerge,
microbiologists strive to find cures
new vaccines appear to be the
best hope as microorganisms
continue to develop drug resistance
Relevance of
Microbiology
Indigenous microflora
Aid in digestion
Nutrient cycles
Food chain - planktons
Decomposers or Saprophytes
Bioremediation
Antibiotic production
Cell models E. coli O157:H7
Genetic Engineering
Beneficial Uses of
Microorganisms
Environmental
make nutrients from non-living sources
for plants and animals (saprophytes)
provides energy to our ecosystem
helps in nutrient cycle
planktons helps in food chain
killed more
people than
WW1, WW2,
Korea, and
Vietnam
combined
Plague bacteria
Medical Microbiology
resurgence of new diseases is due to any or all of
the following
resistance to antibiotics
increase in foreign travel
increase in foreign visitors
parents becoming lax on childhood vaccinations
Medical Microbiology
emerging diseases due to
changing lifestyles
Smallpox virus
Microbiology Successes
potato famine in Ireland
1850s
late blight fungus
Blight fungi