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Institute of Food Science and Technology (IFST), Bangladesh Council of Scientific and Industrial
Research (BCSIR), Dhaka, Bangladesh.
2
BCSIR Laboratories Chittagong, Bangladesh Council of Scientific and Industrial Research (BCSIR),
Bangladesh.
3
Enteric & Food Microbiology Laboratory, Laboratory Sciences Division, ICDDR,B. Dhaka, Bangladesh.
ABSTRACT
Nucleic acid sequence-based amplification (NASBA) is a sensitive, isothermal, transcription-based
amplification system specifically designed for the detection of RNA targets. In some NASBA systems, DNA
can also be amplified. This amplification system uses a battery of three enzymes (avian myeloblastosis virus
reverse transcriptase, RNase H and T7 RNA polymerase) leading to main amplification product of singlestranded RNA. Expensive equipments are not necessary to acquire a high level of precision. NASBA is an
established diagnostic tool in clinical use, with a theoretically bigger analytical sensitivity than reverse
transcription-polymerase chain reaction (RT-PCR) for pathogen detection. It has a potential for detection of
viable cells through selective amplification of messenger RNA, even in a background of genomic DNA, which
PCR does not possess. In the future, NASBA could be used to identify and subsequently quantify
microorganisms (even those which cannot be readily cultured) and would be very efficient as routine diagnostic
procedures.
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(Blank et al., 2002). Additionally, a multiplex realtime NASBA was described for the simultaneous
detection and quantification of CMV encoded IE 1
and pp67 mRNA (Greijer et al., 2002).
9.2.4. Flavivirus Genomic RNA
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12.0 CONCLUSION
NASBA method is a powerful innovative gene
amplification technique emerging as a simple rapid
diagnostic tool for early detection and identification
of microbial diseases. The use of NASBA could
contribute to the development of an affordable,
portable and easy to use pathogen detection kit,
suitable even for less well equipped laboratories.
13.0 ACKNOWLEDGEMENT
Authors are indebted to Bangladesh Council of
Scientific and Industrial Research (BCSIR) for
providing an excellent job environment for study and
research.
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