Professional Documents
Culture Documents
Original Article
INTRODUCTION
North East Indian orchids occupy a place of pride in
floriculture for their aesthetic values with most
beautiful and long lasting quality. This region is one
of the richest regions in orchid diversity with 876
species under 150 genera, comprising of more than
50% of the total Indian orchids, out of total 85,000
species and 800 genera all over the world [2]. Several
species of Indian orchids, like Cymbidium, Dendrobium, Paphiopedillum and Vanda, available in
Northeast India, are of great demand in the country
and abroad [6]. Rhyncostylis retusa locally known
as Kopou phul in Assam is invariably adorned by
ladies during the famous Bihu festival for
ornamentation on their head. This glamorous orchid
has immense importance not only in the cultural front
of Assamese society but also has an impact on the
psyche of the population. Some other rare and showy
species like Coelogyne, Cymbidium, Dendrobium,
Paphiopedillum, Phaius, Rananthera, Vanda etc
of this region, have been listed as threatened and
1585
Knudson
Burgeff
Phytamax
Dendrobium aphyllum
76-100
14
1-25
35
26-50
14
26-50
14
Rhyncostlis retusa
76-100
14
1-25
35
1-25
14
1-25
14
Aerides odoratum
1-25
35
Nil
Nil
1-25
42
1-25
42
76-100
35
Nil
Nil
26-50
35
1-25
35
Cymbidium Longifolium
Knudson
Burgeff
Burgeff + coconut
water* + banana
extract**
Orchid species
Shoot
Root Shoot
Root
Dendrobium aphyllum
77
105
70
Rhyncostylis retusa
77
105
70
Aerides odoratum
98
119
84
Cymbidium longifolium
77
126
70
119
1586
98
Root
84
112
77
105
98
98
119
84
105
105
105
119
98
112
98
126
84
119
Das et al.
Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 1: Protocorm
Fig. 2: Shoot formation
Fig. 3 In vitro hardening
Fig. 4: Hardening in pots
Fig. 5: In vitro plants in field
Fig. 5
1587
REFERENCES
[1] Bhojwani, S.S. and Razdan, M.K. : Plant Tissue
Culture, Theory & Practical, Elsvier Science
publication company Inc New York, USA (1983).
[2] Bose, T.K., Bhattacharjee, S.K., Das, P. and Basak,
K.C.: Orchids of India, Naya Prakashan, Calcutta, India
(1993).
[3] Chin C. and Doris Chi-Ning Chang. : Botan. Bull. Acad.
Sinica, 45: 143-147 (2004).
[4] Devi, J., Nath, M., Devi, M. and Deka, P.C.: National
Seminar on Current Research Trend in Indian
Orchids with Special Reference to Tissue Culture.
NEHU, Orchid Society of India, Shillong, pp 13 (1988).
[5] Hazarika, R.B. and Sharma, C.M. : J. Orchid Soc. of
India, 9(1-2): 51-54 (1995).
[6] Hegde, S.N.: Orchids of Arunachal Pradesh, Forest
Department, Arunachal Pradesh, Itanagar (1984).
[7] Johnson, T.R. and Kane, M.E. : Plant cell, Tissue and
organ culture, 91 (3), 251-261 (2007).
[8] Johnson, T.R., Stewart, Scott L., Dutra, Daniel, Kane,
Michael E. and Richardson, L.: Plant cell, Tissue and
organ culture, 90, 313-323 (2007).
[9] Morel, G.M. : Am. Orchid Society, Bull. 29: 495-497
(1960).
[10] Stewart, S. L. and Kane, M.E.: Plant cell, Tissue and
organ culture, 86: 147-159 (2006).
ACKNOWLEDGEMENTS
The authors are thankful to Director, Defence
Research Laboratory, Tezpur (Assam) and former
Director Dr. S. N. Dube for their constant guidance
and support to carry out the research work.
1588