Initial

Final
Volume of
Temperat
burette
burette
base
ure (oC
Trial
reading
reading
required
Average
o
3
3
3
0.5 C)
(cm 0.05 (cm 0.05
(cm 0.1
cm3)
cm3)
cm3)
1
0.00
12.00
12.0
25
2
12.50
24.70
12.2
12.1
3
24.70
37.00
12.3
1
0.00
9.40
9.4
50
2
9.40
18.70
9.3
9.37
3
18.70
28.10
9.4
1
0.00
6.80
6.8
75
2
6.80
13.30
6.5
6.70
3
13.30
20.10
6.8
1
37.00
39.00
2.0
100
2
39.00
41.10
2.1
2.07
3
41.10
43.20
2.1
3
Table 1: Titration raw data of 50cm water samples against sodium
thiosulfate
Calculating the uncertainty of 0.005M sodium thiosulfate solution used for
titration

Mass of sodium thiosulfate used

uncertainty: 2.53%)

Molar mass

Mole

Volume

158.11

0.40 g

( 0)

mass
0.395
=
=0.0025
molar mass 158.11
500

( 0.01) (Percentage

( 0.001)

cm3 ( 0.25)

0.5 dm3 (Percentage uncertainty: 0.05%)

mole
0.0025
=
=0.005
volume
0.5

[MnSO4]

Percentage uncertainty:

Absolute uncertainty:

moldm-3 ( 0.000129)

2.58

0.005

2.58
=0.000129
100

Data processing
Overview

Volume of thiosulfate solution used: 12.17 cm3 (0.15) (Percentage

uncertainty: 1.23%)

Moles of thiosulfate used can be calculated by the following equation.

Moles of thiosulfate used =
Volume of thiosulfate solution used concentration of thiosulfate solution
1000

The ratio of of dissolved oxygen to thiosulfate is 1:4 according to the

equation of the Winklers method, 4 moles of S2O32- are used for every
1 mole of O2 in the water sample.
2Mn2+(aq) + O2(g) + 4OH-(aq) 2MnO2(s) + 2H2O(l)
MnO2(s) + 2I-(aq) + 4H+(aq) Mn2+(aq) + I2(aq) + 2H2O(l)
2S2O32-(aq) + I2(aq) 2I-(aq) + S4O62-(aq)
Hence, the moles of dissolved oxygen in water sample =
moles of thiosulfate used
4

The volume of water sample used for titration is 50 cm3 (0.06)

(Percentage uncertainty: 0.12%). Hence, the mol/cm3 of dissolved
oxygen found in a water sample could be calculated as follow.
moles of dissolved oxygen a water sample
mol /cm3 of dissolved oxygen=
50

The mol/L of dissolved oxygen is thus =

mol /cm3 of dissolved oxygen 1000

The concentration of dissolved oxygen in a water sample can be

calculated as below.
Concentration( g /L)=mol / Lof dissolved oxygen Ar of O 2

Since the amount of dissolved oxygen in g/L is too small, parts per
million (ppm) should be used.
Concentration( ppm)=concentration(g/ L) 1000

Sample calculation (25oC)

Volume of thiosulfate solution used: 12.17 cm3 (0.15) (Percentage

uncertainty: 1.23%)
12.17 0.005

Moles of thiosulfate used =

1000
= 6.085 105 mol
( 2.32 106 ) ( 3.81 )

Moles of dissolved oxygen in water sample =

6 . 085 105
4
= 1. 521 105 mol

( 5.80 107 ) ( 3.81 )

Volume of water sample: 50 cm3 (0.06) (Percentage uncertainty:

0.12%)

Mole per cm3 of dissolved oxygen =

1.521 105
50
=

3.04 3 107 mol /cm3 ( 1.20 108 )

Mole per liter of dissolved oxygen =

3.043 10 1000
=

3.04 3 104 mol / L(1.20 105)

Concentration (g/L) of dissolved oxygen = 3.043 104 32

=
3
4
9.736 10 g/ L ( 3.84 10 )
Concentration of dissolved oxygen in ppm =

9.736 10 1000
= 9.736 ppm

Presentation of calculations
Temperat
ure of
water

Mean
volume of
thiosulfate

Moles of
thiosulfat
e used

Moles of
dissolved
oxygen

Mole per
liter of
dissolved

Concentrat
ion of
dissolved

sample
(oC
0.5oC)

used
(0.15cm3
)

25

12.17

50

9.36

75

6.70

100

2.07

6.085
10-5
4.680
10-5
3.350
10-5
1.035
10-5

x
x
x
x

present

oxygen
present

1.521
10-5
1.170
10-5
8.375
10-6
2.588
10-6

3.043
10-4
2.340
10-4
1.675
10-4
5.175
10-5

x
x
x
x

x
x
x
x

oxygen
present
(ppm)
9.736
7.488
5.360
1.656