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(INTRODUCTION: ANALYTICAL
CHEMISTRY)
Lesson Outcomes:
Differentiate between qualitative & quantitative
analysis
Explain the steps involve in quantitative analysis
Calculate the concentration of solution
Explain the preparation of solution
Types of Analysis
Analytical chemists perform two main tasks:
Qualitative
Quantitative
Steps in Analysis
Defining the problem
Eliminating interferences
Performing the Measurement
1. Defining Problems
The goal of every chemical analysis is to obtain the
required information within a period of time
acceptable to the customer.
Many problem do not require complete
identification and many cases require only a general
classification.
It is important for the analyst to determine the
information required by the client.
For example, in water analysis, only the total
hardness is required rather than the concentration
of individual Ca2+ and Mg2+ ion concentration is not
necessary.
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Questions to be considered:
What is the concentration range (detection limit)
What accuracy is required (depends on the
problem)
What elements or compounds are involved
How complex is the mixture (selectivity,
separation)
How many samples are involved (analysis time)
What methods are available to you !!!
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5. Eliminating Interferences
Impurities may contribute to (or decrease) the
spectroscopic signal used to calculate the
concentration e.g. Si produces the same blue colour
as PO43- with molybdenate reagent
These interferences must be identified and their
effect eliminated which can present a difficult
problem
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During pretreatment:
reduce and remove interferences
adjust analyte concentrations to a range suitable for
measurement
produce species from analyte that have
quantitatively measurable properties
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Moles =
grams
formula weight (g/mol)
Milimoles =
miligrams
formula weight (mg/mmol)
1 mole = 1000 milimoles
Sometimes it is more convenient to make calculations
with milimoles (mmol) rather than moles, where the
1 milimole = 0.001 mole
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Example:
Calculate the number of moles in 500 mg Na2WO4
Solution:
Moles =
grams
formula weight
500 mg/1000
293.8
= 0.0017 mol
1 g = 1000 mg
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Example:
How many mg are in 0.250 mmol Fe2O3?
Solution:
Milimoles =
miligrams
formula weight (mg/mmol)
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Example:
How many moles and milimoles of benzoic acid
(Molar mass =122.1 g/mol) are contained in 2g of
pure acid?
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Solution:
Mole = mass (g)/molar mass (g/mol)
= 2g of benzoic acid x 1 mol benzoic acid
122.1 g benzoic acid
1 mole = 1000
= 0.0164 mol benzoic acid
milimole
Milimole benzoic acid
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Exercises:
1. Calculate the number of moles for 3.4 g of carbon
atoms.
2. What is the mass of 0.30 moles of CH4?
3. How many moles of Cl- is present in 0.34 moles of
CaCl2?
4. What is the mass of H in 2.5 moles of H2O?
5. What is the mass of O in 3.0 moles of CO2?
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Example:
What is the molarity of a solution of 0.60 g NaCl in
100 mL of solution? Given, MW NaCl = 58.5 g/mol
Solution:
No. of mol =
0.60 g
58.5 g/mol
= 0.0102 mol
Molarity
= 0.0102 mol
0.1 L
= 0.102 M
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Example:
If you have 10.0 grams of Br2 and dissolve it in 1.00 L of
cyclohexane, what is the molality of the solution? The
density of cyclohexane is 0.779 kg/l at room
temperature.
Solution:
Mole of Br2
10 g / (159.8 g/mole) = 0.063 moles Br2
Convert the volume of solvent to the weight of solvent
using the density
1.00 L x 0.779 kg/l = 0.779 kg
Divide the two to get the molality 0.063 moles Br2/ 0.779
kg cyclohexane
= 0.080 molal
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Example:
Calculate the molarity of a potassium hydroxide
(KOH) solution having a density of 1.28 g/mL and
containing 25 percent (by weight) of the dissolved
compound.
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Solution:
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Example:
As a student, you are given copper (II) sulphate pentahydrate
(CuSO4.5H2O, MW 249.69 gmol-1). Describe how you can
prepare a 500 mL standard solution of 10.00 mM Cu2+ from
the reagent.
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Exercise:
How many milliliters of concentrated sulfuric acid,
94.0% (g/100 g solution), density 1.831 g/cm3, are
required to prepare 1 L of 0.100 M solution?
Answer: 5.71 mL
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Other Representations of
Concentration
Percent Concentration:
Weight % (w/w) = Weight solute (g)
x 100
Weight of solution (g)
Volume % (v/v) = Volume solute (mL) x 100
Volume of solution (mL)
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the
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Example:
Briefly explain how to prepare 1.0 L 10.50% (w/v)
aqueous CH3CH2CH2OH
Solution:
%w/v =
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Parts per
million
Parts per
billion
ppm
ppb
Units
w/w
w/v
v/v
g/g
g/mL
nL/mL
mg/kg
mg/L
L/L
ng/g
ng/mL
nL/L
g/kg
g/L
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Example:
What is the molarity of K+ in a solution that
contains 63.3 ppm of K3Fe(CN)6 (329.3 g/mol)?
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Solution:
Because the solution is so dilute, assume its density = 1.00
g/mL. So,
63.3 ppm K3Fe(CN)6 = 63.3 mg K3Fe(CN)6 /L
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Exercise:
250 mL 50 ppm of Ca2+ from the solid chemical
Ca(NO3)2
Answer: 0.0513 g
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Dilution
Dilute solution (with low concentration) can be
prepared from a more concentrated solution
A known volume of the concentrated solution
can be transferred into a new flask and diluted to
the required volume or weight
M1V1 = M2V2
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Example:
The laboratory grade concentrated HCl has a
concentration of 12.1 M. What a volume of the
concentrated acid is necessary to prepare 500 mL of 0.100
M HCl?
Solution:
M1V1 = M2V2
(12.1 M) x V1 = (0.100 M) x 500 mL
V1 = (50.0) / 12.1 = 4.13 mL.
Procedures:
1. Dilute 4.13 mL of concentrated acid in a 500-mL VF
2.Dilute with distilled water to the 500-mL mark
3.Invert the flask several times to ensure complete mixing.