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Study Guide B
Answer Key
7. The sugar-phosphate backbone is like the
twisting handrails of the staircase, and the
nitrogen-containing bases are like the steps
that connect the railings to each other.
8. Because A only pairs with T and C only
pairs with G, the amount of A will be equal
to the amount of T, and the amount of C will
be equal to the amount of G.
7.
8.
9.
10.
11.
Mice lived
Mice died
Mice lived
Mice died
the S form
Griffith concluded that a transforming
principle had been transferred from the heatkilled S bacteria to the live R bacteria.
Result: DNA is present, but protein is not.
Averys Question: What is the chemical
makeup of the transforming principle?
Result: enzymes that destroy DNA
sulfur, phosphorus
phosphorus, sulfur
Experiment 1: sulfur; radioactivity absent.
Experiment 2: phosphorus; radioactivity
present
A bacteriophage is a virus that infects
bacteria. It consists of a DNA molecule
surrounded by a protein coat.
1.
2.
3.
4.
5.
6.
9.
10.
11.
12.
13.
SECTION 5. TRANSLATION
replication (nucleus)
transcription (nucleus)
translation (cytoplasm)
Contains the sugar ribose
Has the bases A, C, G, and T
Typically single-stranded
RNA polymerase
A large transcription complex, including
RNA polymerase and other proteins,
assembles at the start of a gene and begins to
unwind the DNA. Using one strand of the
DNA as a template, RNA polymerase
strings together a complementary strand of
RNA. The RNA strand detaches from the
DNA as it is transcribed, and the DNA zips
back together.
mRNA: intermediate message that is
translated to form a protein; rRNA: forms
part of ribosomes; tRNA: brings amino
acids from the cytoplasm to a ribosome to
help make the growing protein
Both occur within the nucleus of eukaryotic
cells, are catalyzed by large enzymes,
involve unwinding of the DNA double helix,
involve complementary base pairing of the
DNA strand, and are highly regulated by
the cell.
Replication occurs only once during each
round of the cell cycle and makes a doublestranded copy of all the DNA in a cell.
Transcription occurs repeatedly throughout
the cell cycle to make proteins, rRNAs, and
tRNAs as needed by a cell. Transcription
makes a single-stranded complement of only
a particular DNA sequence.
mRNA is a form of the DNA message that
tells the cell what type of protein to make.
rRNA is a key component of ribosomes.
tRNA transfers, or carries, amino acids from
the cytoplasm to the ribosome.
the process of copying a sequence of DNA
to produce a complementary strand of RNA
ii
SECTION 7. MUTATIONS
1. point mutation/substitution; frameshift
mutation
2. gene duplication; translocation
3. chromosomal mutation
4. unequal crossing over
5. the attachment of a piece of one
chromosome to a nonhomologous
chromosome
6. Answers will vary.
7. Answers will vary.
8. noncoding regions
9. premature stop codon
10. no change
11. lack of regulation
12. altered splice site
13. germ cells/gametes
14. no
15. an agent in the environment that can change
DNA
16. UV light can cause neighboring thymine
nucleotides to break their hydrogen bonds to
adenine and bond with each other instead.
17. a change in an organisms DNA
18. a frameshift mutation
iii
Study Guide B
KEY CONCEPT
bacteriophage
MAIN IDEA: Griffith finds a transforming principle.
Write the results of Griffiths experiments in the boxes below.
Experiments
Results
Found live S
bacteria in the
mices blood
7. Avery and his team isolated Griffiths transforming principle and performed
three tests to learn if it was DNA or protein. In the table below, summarize
Averys work by writing the question he was asking or the results of his
experiment.
Averys Question
Results
Bacteriophage
Bacteria
Experiment 1
Experiment 2
Vocabulary Check
11. Explain what a bacteriophage is and describe or sketch its structure.
____________________________________________________________
Study Guide B
KEY CONCEPT
nucleotide
double helix
MAIN IDEA: DNA is composed of four types of nucleotides.
In the space below, draw a nucleotide and label its three parts using words and
arrows.
Vocabulary Check
7. Explain how the DNA double helix is similar to a spiral staircase.
____________________________________________________________
____________________________________________________________
8. How do the base pairing rules relate to Chargaff s rules?
____________________________________________________________
____________________________________________________________
Study Guide B
KEY CONCEPT
replication
DNA polymerase
10.
11.
_______________
_______________
12.
_______________
_______________
_______________
_______________
Vocabulary Check
15. Explain what DNA polymerase is by breaking the word into its parts.
____________________________________________________________
16. Write a short analogy to explain what replication is.
____________________________________________________________
Be Creative
17. People sometimes like to display bumper stickers that relate to their trade or
field of study. For example, a chemist may have a bumper sticker that says It
takes alkynes to make the world. Then, chemists or other people who know
that an alkyne is a molecule that contains carbon atoms joined by a triple bond
get a nice little chuckle out of the play on words. Use your knowledge of DNA
replication to write a bumper sticker.
____________________________________________________________
____________________________________________________________
Section 4: Transcription
Study Guide B
KEY CONCEPT
central dogma
RNA
transcription
RNA polymerase
MAIN IDEA: RNA carries DNAs instructions.
Label each of the processes represented by the arrows in the diagram below.
Write where each of these processes takes place in a eukaryotic cell in
parentheses.
1. _______________
DNA
RNA
2. _______________
_______________
Proteins
3. _______________
_______________
DNA
RNA
6. Typically double-stranded
Function
mRNA
rRNA
tRNA
10. List two ways that the processes of transcription and replication are similar.
____________________________________________________________
11. List two ways that the end results of transcription and replication differ.
____________________________________________________________
Vocabulary Check
12. How does the name of each type of RNA tell what it does?
____________________________________________________________
____________________________________________________________
____________________________________________________________
13. What is transcription?
____________________________________________________________
Houghton Mifflin Harcourt Publishing Company
Section 5: Translation
Study Guide B
KEY CONCEPT
translation
stop codon
codon
start codon
anticodon
1. What is translation?
____________________________________________________________
2. What is a codon?
____________________________________________________________
3. Would the codons in Figure 5.1 be found in a strand of DNA or RNA?
____________________________________________________________
4. What is a reading frame?
____________________________________________________________
Refer to Figure 5.1 to complete the table below.
Codon
5. AGA
6. UAG
7.
tryptophan (Trp)
8. GGA
MAIN IDEA: Amino acids are linked to become a protein.
Ribosome assembles on
start codon of mRNA
strand.
A.
B.
C.
Vocabulary Check
13. What are AGG, GCA, and GUU examples of?
____________________________________________________________
14. What is a set of three nucleotides on a tRNA molecule that is complementary
to an mRNA codon?
____________________________________________________________
15. What do codons code for in addition to amino acids?
____________________________________________________________
10
Study Guide B
KEY CONCEPT
promoter
exon
operon
intron
MAIN IDEA: Prokaryotic cells turn genes on and off by controlling transcription.
The
repressor
medium
continues
without lactose to bind to
added
the
operator.
5.
6.
8.
The
resulting
transcript is
translated
into 3
enzymes.
Bacteria
growing
in culture
7.
medium
with lactose
added
9.
11
10. Why do the cells in your body differ from each other?
____________________________________________________________
11. What role do transcription factors play in a cell?
____________________________________________________________
12. What is a TATA box?
____________________________________________________________
13. What is sonic hedgehog an example of?
____________________________________________________________
The diagrams below represent unprocessed and processed mRNA in a eukaryotic
cell. Using the diagrams as a reference, fill in the legend with the corresponding
element: cap, exon, intron, tail.
Legend
Unprocessed mRNA
Processed mRNA
Vocabulary Check
14. What is the difference between an exon and an intron?
____________________________________________________________
____________________________________________________________
15. Make an analogy to help you remember what a promoter is.
____________________________________________________________
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Section 7: Mutations
Study Guide B
KEY CONCEPT
Mutations are changes in DNA that may or may not affect phenotype.
VOCABULARY
mutation
frameshift mutation
point mutation
mutagen
MAIN IDEA: Some mutations affect a single gene, while others affect an entire
chromosome.
AGGCGTCCATGA
6.
7.
13
10.
12.
Name _____________________________ Class _________________ Date _________________
nonfunctional protein
coding regions
Point
mutations
result
in
9.
10.
may
occur
in
11.
result
in
8.
12.
no change
13. For a mutation to be passed to offspring, in what type of cell must it occur?
____________________________________________________________
MAIN IDEA: Mutations can be caused by several factors.
14. Can DNA polymerase catch and correct every replication error?
____________________________________________________________
15. What is a mutagen?
____________________________________________________________
16. How does UV light damage the DNA strand?
____________________________________________________________
____________________________________________________________
Vocabulary Check
17. What is a mutation?
____________________________________________________________
18. If a nucleotide is deleted from a strand of DNA, what type of mutation has
occurred?
____________________________________________________________
Houghton Mifflin Harcourt Publishing Company
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