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IFCC
Cat.No
142 016
142 000
142 001
142 002
142 003
142 008
142 004
142 007
142 025
Package Size
7 x 10 mL
5 x 20 mL
2 x 50 mL
4 x 100 mL
4 x 50 mL
4 x 80 mL
4 x 100 mL
4 x 70 mL
5 x 20 mL
(Hit I)
(Hit II)
(Hit II)
(AU)
(LW)
2 x 7 mL
2 x 10 mL
1 x 20 mL
4 x 20 mL
2 x 20 mL
4 x 16 mL
4 x 20 mL
3 x 20 mL
2 x 10 mL
METHOD
PRINCIPLE
Kinetic determination of Alaninaminotransferase
(ALAT) based upon IFCC recommendations:
Pyruvat + NADH + H+
PROCEDURES
Reagents can be used manually (see method below) and
on most analyzers. Applications are available on request.
Wavelength
:
Temperature
:
Cuvette
:
Read against air.
L-Alanin+ 2-Oxoglutarat
ALAT = Alaninaminotransferase
LDH = Lactate dehydrogenase
REAGENTS COMPOSITION
R1:
100 mmol/l
R2:
L-Alanine
LDH ( Lactatdehydrogenase)
2-Oxoglutarate
500 mmol/l
1200 U/l
15 mmol/l
NADH
0,18 mmol/l
- PRECAUTIONS
- For in vitro diagnostic use only.
- The reagents contain < 0,95g/L sodium azide.
Avoid contact with skin and/or mucous
membranes !
- Use clean or single use glass/plastic material
only to avoid contaminations.
STABILITY OF REAGENTS
When stored at 2 8 C and protected from light,
the reagents are stable until the expiry date printed
on the labels.
PREPARATION OF WORKING REAGENT
- For Start Reagent-procedure
reagents R1 and R2 are ready for use
- For Sample Start-procedure (monoreagent)
mix R1 + R2 = 5 + 1
Stability : 1 month at 2 8 C
5 days at 18 25 C
37C,
1 cm light path
1 mL
100 L
1 mL
100 L
R2
:
200 L
Mix and after a 1 minute incubation, measure the
change of absorbance per minute (A/min) during
3 minutes.
CALCULATION FACTORS
Monoreagent
340 nm : Activity (U/L) = A/min. x 1 745
334 nm : Activity (U/L) = A/min. x 1 780
365 nm : Activity (U/L) = A/min. x 3 235
Start reagent
2064
2103
3824
SAMPLES
Serum free of hemolysis , plasma (EDTA/heparin)
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BIBLIOGRAPHY
- Sensitivity
The sensitivity / lower detection limit is < 1U/L
3.
1.
2.
- Precision
Within-run reproducibility
Within series
Mean
n = 11
Value
[U/L]
Sample 1
38,4
Sample 2
143
Sample 3
111
Standarddeviation
[U/L]
0,51
1,95
1,27
Between-run reproducibility
Day to day
Mean
n = 11
Value
[U/L]
Sample 1
36,0
Sample 2
146
Sample 3
109
Standarddeviation
[U/L]
0,78
0,94
1,40
CV
[%]
1,32
1,36
1,14
4.
CV
[%]
2,15
0,65
1,28
SYMBOLS USED
- Correlation
A comparative study has been perfo ssion are as
follows:rmed between the Greiner method and
another commercial reagent on 30 human serum
samples. The parameters of linear regre
Correlation coefficient
Linear regression:
Use by
r = 0.998
y = 0,967x + 3,365 U/L
Batch Code
Temperature limitation
INTERFERENCES
- Ascorbic Acid: no interference until 100 mg/L
- Triglycerides: no interference until 1000 mg/dL
- Bilirubin:
no interference up to 40 mg/dL
- Hemoglobin:
no interference up to 400 mg/dL
06/2013//rev03/2014
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