QUESTION BANK FOR GENETIC

ENGINEERING
Short Answers questions
1. Define Mushroom?
2. Define macrofungal and its applications?
3. Write any two-mycorrhizal species with eg?
4. What is meant by plasmogamy and karyogamy?
5. Explain homothallism and heterothallism?
6. Write short notes on mushroom nutritive value with percentage.?
7. Which mushroom controls blood pressue and numbness of hands?
8. Define Phanerochaete. Chrysosporium?
9. Define Phanerochaete flavido-alba?
10. Define Trametes versicolor?
11. Define Pleurotus ostreatus?
12. Define Lentinus squarrosulus?
13. Define Pleurotus pulmonarius?
14. Explain the major phases of mushroom cultivation?
15. What is meant by MEA?
16. Define Spawn substrate?
17. What is meant by multiplication of cultures?
18. What is meant by spawn quantity?
19. Write any two commercial spawn supplies?
20. What is meant by composting? With eg.
21. Explain spent compost?
22. Write short notes on spawn running?
23. Define management of fruiting?
24. Explain any three achievement on composting?
25. Write short notes two different types of composting in
A.bisporus?
26. Write short notes on Lentinula edodes?

Paragraph questions
1. Write any five advantages of mushroom cultivation in
a details manner?
2. Explain the four different types of mushroom and
their values?
3. Explain the morphology and anatomy of Agaricus
bisporus?with neat diagram?
4. Explain the morphology and anatomy of Volvariella
volvacea with neat diagram?
5. Explain the morphology and anatomy of Pleurotus
with neat diagram?
6. Give a detailed account on mushroom genetics and
breeding with neat diagram?
7. Explain with neat diagram mushroom life cycle
(theory explanation is mandate)
8. Explain homothallism and heterothallism and
pseudothallism with neat diagram?
9. Write any five steps involved in strain improvement
and their detailed explanation?
10. Explain the bed method in mushroom
production?
11. Explain the polytene bag method in mushroom
production?
12. Explain any Eight-medicinal value of
mushrooms?
13. Write any five mushrooms bioremediation?
14. Explain any three different sources of mushroom
culture?
15. Write any two media preparation for growing
mushroom in lab conditions?
16. Write the steps involved in preparation of mother
spawn preparation?
17. Write the steps involved in preparation of
planting spawn preparation?
18. Explain any ten cleanliness which is followed in
mushroom cultivation?
19. Write any five steps followed in mushroom
spawn handling?
20. Explain in detail mushroom substrate compost
for (i) Agaricus bisporus
(ii) volvariella volvacea (iii)Lentinula edodes (iv)
pleurotus spp.

21. Give a detailed account mushroom disease and

their control methods at least five ?
22. Explain any five rememberable points on post
harvest handling?
 23. What are different phases of mushroom
production followed in A.bisporus?
24. Explain the different formula of spawn
substrate(I) mother spawning
(ii) Planting spawn
25. Give a detailed account (i) cottage scale
cultivation and (ii) commercial scale cultivation in
lentinula edodes.
26. Explain pleurotus and volvariella cultivation
methods?

AKSUM UNIVERSITY
COLLEGE OF NATURAL AND COMPUTATIONAL SCIENCES
DEPARTMENT OF BIOTECHNOLOGY
MUSHROOM PRODUCTION AND TECHNOLOGY FINAL
EXAMINATION
4TH YEAR B.SC BIOTECHNOLOGY

STUDENT NAME_______________________________

ID.NO: __________________
***************************************************************
****
INSTRUCTIONS
1. SWITCH OFF YOUR MOBILE PHONES INSIDE THE
EXAMINATION HALL
2. No Discussion inside the examination hall
3. Any kind of malpractices will nullify your result
4. Dont open your question paper until the examiner
told to do so.
5. Check your question paper totally contains part-I to
part

Exam committee members

Instructors Name Signature
1.
2.
3.

Dept Seal.
Part-I Choose the correct answer.
1. Winter mushroom is otherwise known as_________________
A) Lentinus edodes B) A.bisporus (C)Pleurotus D)
Flammulina

2. The mushroom which can decolorize olive oil mill waster

water is ___________________
A)Phanerochaete chrysosporium B) P.ostreatus C)
P.Flavido alba
D) Pleurotus pulmonarius.

3. Which mushrooms is used to mineralize soil contamination

of crude oil __________________
A) P.pulmonarius B) Lentinus edodes C) L.squarrosulus.

4. Which type of mushroom is considered as poisonous

mushroom
A) P.pulmonarius B) L.edodes C) Amanitia
muscaria
D) Amanitia phalloides
5. Mushrooms contains about ______________ % carbohydrate and
_______% Fat
A) 6.2 & 0.3% B) 4.2 &1.2 C) 4.2& 0.3% D) None
of the above.

6. Which type of mushroom has antiviral and antitumour

property
A) L.edodes B) amanitia muscarica C) A.bisporous D)
All are correct

7. Agaricus bisporus grows on fermented compost which is traditionally

developed from ______________ with ____________
A) Cotton husk & Water B) soil & straw ( C) Wheat straw & cow dung
D) Wheat straw and horse manure.

8. Inky caps a type of mushroom disease caused

by______________________

A) Dactylium B) Papulospora C) Coprinus D) Virus.

9. How to control Dry bubble disease in

mushroom____________________
A) Pasteurized at 60 o c B) Autoclave at 60oc C) Hot air oven
at 60oc D) None of the above
10. Brown blotch is a disease caused by ___________
A) Fungi B) Virus C) bacteria D) none of the
above.
PART-II FILL UP THE BLANKS

1. Drippy gill disease caused by ___________________

2. Mummy disease in mushroom caused by
________________
3. Composting achievement ______________ and
_______________
4. Agaricus composting types_______________ and
__________________
5. Truffle disease caused by____________________
6. Infection of mushroom in wet bubble leads to
mushroom _______________
7. If nematodes affect mushroom ________________ and
_____________ is used to reduce nematode infection .
8. Champignon mushroom is otherwise known as________________
9. Which mushroom grows on dead trees and
trunks______________
10. The optimum temperature for spore germination
in L.edodedis ____________.

Part-III Short answers

(Each question carries 2 points)

1. Write short notes on mushroom nutritive value with

percentage?

2. Define Trametes versicolor?

3. Explain spent compost?

4. Define Pleurotus pulmonarius?

5. What is meant by composting with eg?

Part-IV Paragraph Answers (Each question carries 5

points)(Answer any four questions only)

1. Explain with neat diagram Mushroom Life cycle

(theory explanation is mandate)
Part-IV Paragraph Answers (Each question carries 5
points) Answer any four questions only)

2. Give a detailed account on cottage scale cultivation

and commercial scale cultivation in L.edodes?
Part-IV Paragraph Answers (Each question carries 5
points) Answer any four questions only)

3. Explain homothallism and heterothallism and

pseudothallism with neat diagram?
Part-IV Paragraph Answers (Each question carries 5
points) Answer any four questions only)

4. Explain any ten cleanliness which is followed in

mushroom cultivation?
Part-IV Paragraph Answers (Each question carries 5
points) Answer any four questions only)
5. Write the steps involved in mother spawn
preparation (composition should be exact)?