This document appears to be a quiz for a cell and molecular biology course. It contains 10 multiple choice questions covering various topics related to gene expression and regulation in both prokaryotes and eukaryotes. The questions assess students' understanding of concepts like promoter and terminator structures, transcriptional and translational processes, and the impact of mutations on gene function. The document includes sections for students to print their name, student number, and sign a pledge affirming their own work.
This document appears to be a quiz for a cell and molecular biology course. It contains 10 multiple choice questions covering various topics related to gene expression and regulation in both prokaryotes and eukaryotes. The questions assess students' understanding of concepts like promoter and terminator structures, transcriptional and translational processes, and the impact of mutations on gene function. The document includes sections for students to print their name, student number, and sign a pledge affirming their own work.
This document appears to be a quiz for a cell and molecular biology course. It contains 10 multiple choice questions covering various topics related to gene expression and regulation in both prokaryotes and eukaryotes. The questions assess students' understanding of concepts like promoter and terminator structures, transcriptional and translational processes, and the impact of mutations on gene function. The document includes sections for students to print their name, student number, and sign a pledge affirming their own work.
CELL & MOLECULAR BIOLOGY: Quiz No. 2, 30 September 2014
Name
(print)
_________________________
Student
Number
_____________________
I
pledge
that
the
answers
on
this
quiz
are
the
sole
efforts
of
myself.
Violations
of
this
pledge
will
be
adjudicated
by
the
Virginia
Tech
Honor
System.
Include
on
Opscan!
Test
Version
A
Signature
_______________________________
Please
provide
the
most
accurate
answer
for
the
following
questions
and
statements.
Jim Tokuhisa 9/26/2015 15:15 Comment [1]: This
quiz
was
pretty
1. Which
of
the
following
RNAs
has
a
translator
activity?
challenging
with
many
questions
involving
A. mRNA
applications
of
concepts.
B. miRNA
C. rRNA
D. siRNA
E. tRNA
2. If
a
gene
has
a
TATA
box,
would
you
expect
the
promoter
to
bind
a
sigma
factor?
Jim Tokuhisa 9/26/2015 15:04 A. Yes
Comment [2]: I
like
to
compare
processes
B. No
between
prokaryotes
and
eukaryotes.
3. You
have
sequenced
the
DNA
of
a
virus
that
is
pathogenic
to
humans.
In
the
DNA
you
find
several
genes
that
have
the
promoter
and
terminator
structure
of
a
eukaryotic
gene.
Is
this
a
real
finding
or
is
the
virus
DNA
contaminated
with
human
genes?
What
do
you
do?
Jim Tokuhisa 9/26/2015 15:09 A. Redo
the
genome
sequencing
with
a
new
batch
of
DNA
because
the
Comment [3]: Recall
and
apply
the
viral
first
DNA
preparation
was
contaminated
with
eukaryotic
DNA.
reproductive
cycle
and
behavior.
B. Try
to
relate
the
gene
sequence
to
the
pathogenicity
of
the
virus.
4. Which
feature
in
eukaryotic
gene
expression
definitively
prevents
translation
occurring
simultaneously
with
transcription?
A. a
nuclear
envelope
B. a
ribosome
binding
site
C. a
TATA
box
D. introns
E. initiation
factors
F. stop
codon
BIOL 2104 (CRN 81024) Fall 2014 CELL & MOLECULAR BIOLOGY: Quiz No. 2, 30 September 2014
5. What
can
some
eukaryotic
transcriptional
regulators
do
that
prokaryotic
regulators
cannot
do?
A. bind
DNA
B. function
as
repressors
C. interact
with
chromatin
D. interact
with
RNA
polymerase
6. Based
on
germ
line
cell
formation,
higher
plants
are
(A)
more
likely
than
(B)
less
likely
than
or
(C)
equally
likely
as
metazoan
organisms
to
pass
environmentally-induced
mutations
to
their
progeny.
7. Mitochondria
and
chloroplasts
have
there
own
DNA
genomes.
Based
on
their
internal
organization
they
are
predicted
to
have
a
(A)
prokaryotic
or
(B)
eukaryotic
type
of
translation.
8. Mutations
in
the
coding
region
of
a
gene
are
most
likely
to
alter
(A)
levels
of
gene
expression,
(B)
location
of
gene
expression,
or
(C)
specific
activity
of
the
gene
product.
9. If
a
mutation
that
causes
a
neutral
genetic
change
is
very
close,
say
2000
base
pairs,
away
from
a
mutation
that
causes
reduced
fitness,
the
frequency
of
the
neutral
genetic
change
will
most
likely
(A)
increase,
(B)
decrease,
or
(C)
remain
the
same
in
a
breeding
population.
10. Which
enzyme
allows
researchers
to
cut
double-stranded
DNA
at
precise
sequence-specific
locations?
A. DNA
ligase
B. restriction
nuclease
C. reverse
transcriptase
D. Taq
polymerase
BIOL 2104 (CRN 81024) Fall 2014 CELL & MOLECULAR BIOLOGY: Quiz No. 2, 30 September 2014
Name
(print)
_________________________
Student
Number
_____________________
I
pledge
that
the
answers
on
this
quiz
are
the
sole
efforts
of
myself.
Violations
of
this
pledge
will
be
adjudicated
by
the
Virginia
Tech
Honor
System.
Include
on
Opscan!
Test
Version
B
Signature
_______________________________
Please
provide
the
most
accurate
answer
for
the
following
questions
and
statements.
1. Which
of
the
following
RNAs
has
a
translator
activity?
A. tRNA
B. siRNA
C. rRNA
D. mRNA
E. miRNA
2. If
a
gene
has
a
polyadenylation
signal,
would
you
expect
the
promoter
to
bind
a
sigma
factor?
A. Yes
B. No
3. You
have
sequenced
the
DNA
of
a
virus
that
is
pathogenic
to
humans.
In
the
DNA
you
find
several
genes
that
have
the
promoter
and
terminator
structure
of
a
eukaryotic
gene.
What
do
you
do?
A. Redo
the
genome
sequencing
with
a
new
batch
of
DNA
because
the
first
DNA
preparation
was
contaminated
with
eukaryotic
DNA.
B. Try
to
relate
the
gene
sequence
to
the
pathogenicity
of
the
virus.
4. Which
property
of
eukaryotic
genes
prevents
translation
occurring
simultaneously
with
transcription?
A. stop
codon
B. introns
C. initiation
factors
D. a
TATA
box
E. a
ribosome
binding
site
BIOL 2104 (CRN 81024) Fall 2014 CELL & MOLECULAR BIOLOGY: Quiz No. 2, 30 September 2014
5. What
can
some
eukaryotic
transcriptional
regulators
do
that
prokaryotic
regulators
cannot
do?
A. interact
with
RNA
polymerase
B. interact
with
chromatin
C. function
as
repressors
D. bind
DNA
6. Based
on
germ
line
cell
formation,
higher
plants
are
(A)
less
likely
than
(B)
more
likely
than
or
(C)
equally
likely
as
metazoan
organisms
to
pass
environmentally-induced
mutations
to
their
progeny?
7. Mitochondria
and
chloroplasts
have
there
own
DNA
genomes.
Based
on
their
internal
organization
they
are
predicted
to
have
a
(A)
eukaryotic
or
(B)
prokaryotic
type
of
translation.
8. Mutations
in
the
coding
region
of
a
gene
are
most
likely
to
alter
(A)
location
of
gene
expression,
(B)
levels
of
gene
expression,
or
(C)
specific
activity
of
the
gene
product.
9. If
a
mutation
that
causes
a
neutral
genetic
change
is
very
close,
say
2000
base
pairs,
away
from
a
mutation
that
causes
reduced
fitness,
the
frequency
of
the
neutral
genetic
change
will
(A)
decrease
(B)
increase,
or
(C)
remain
the
same
in
a
breeding
population.
10. Which
enzyme
allows
researchers
to
cut
double-stranded
DNA
at
precise
sequence-specific
locations?
A. Taq
polymerase
B. reverse
transcriptase
C. restriction
nuclease
D. DNA
ligase