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Hajime Q. Nakaegawa, Maria Elijah A. Natanawan, Jarvin E. Nipales, Jodine P. Novilla, Jonel G. Nuevo
Group 6, 2A Biochemistry, Faculty of Pharmacy, University of Santo Thomas
ABSTRACT
Phosphoric acid is a weak acid. It is also a polyprotic acid, which means it will give more than one proton (H +) in
solution, each having different equivalence points. The principles behind the Acid-Base Titration were incorporated
during the determination. The experiment was divided into three parts, (1) preparation of the cola sample, (2)
preparation and standardization of the NaOH solution, and (3) the phosphoric acid determination, where its objective
is to determine the phosphoric acid concentration in cola. The equivalence point was determined approximately using
graphical method, including the first and second derivative plot, where the group retrieved 4.50mL as its equivalence
point. The concentration of the phosphoric acid obtained from the experiment was 2.02 x10 -3 M, inferring that the cola
contains only a small amount of phosphoric acid, which should be limited because of its harmful effects to the body.